Differential responses of the antioxidant system of ametryn and clomazone tolerant bacteria
The herbicides ametryn and clomazone are widely used in sugarcane cultivation, and following microbial degradation are considered as soil and water contaminants. The exposure of microorganisms to pesticides can result in oxidative damage due to an increase in the production of reactive oxygen specie...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-11, Vol.9 (11), p.e112271 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 11 |
| container_start_page | e112271 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Peters, Leila Priscila Carvalho, Giselle Martins, Paula Fabiane Dourado, Manuella Nóbrega Vilhena, Milca Bartz Pileggi, Marcos Azevedo, Ricardo Antunes |
| description | The herbicides ametryn and clomazone are widely used in sugarcane cultivation, and following microbial degradation are considered as soil and water contaminants. The exposure of microorganisms to pesticides can result in oxidative damage due to an increase in the production of reactive oxygen species (ROS). This study investigated the response of the antioxidant systems of two bacterial strains tolerant to the herbicides ametryn and clomazone. Bacteria were isolated from soil with a long history of ametryn and clomazone application. Comparative analyses based on 16S rRNA gene sequences revealed that strain CC07 is phylogenetically related to Pseudomonas aeruginosa and strain 4C07 to P. fulva. The two bacterial strains were grown for 14 h in the presence of separate and combined herbicides. Lipid peroxidation, reduced glutathione content (GSH) and antioxidant enzymes activities were evaluated. The overall results indicated that strain 4C07 formed an efficient mechanism to maintain the cellular redox balance by producing reactive oxygen species (ROS) and subsequently scavenging ROS in the presence of the herbicides. The growth of bacterium strain 4C07 was inhibited in the presence of clomazone alone, or in combination with ametryn, but increased glutathione reductase (GR) and glutathione S-transferase (GST) activities, and a higher GSH concentration were detected. Meanwhile, reduced superoxide dismutase (SOD), catalase (CAT) and GST activities and a lower concentration of GSH were detected in the bacterium strain CC07, which was able to achieve better growth in the presence of the herbicides. The results suggest that the two bacterial strains tolerate the ametryn and clomazone herbicides with distinctly different responses of the antioxidant systems. |
| doi_str_mv | 10.1371/journal.pone.0112271 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1621822188</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A418708221</galeid><doaj_id>oai_doaj_org_article_ea3878009d0247d09b7ffd5404c6f538</doaj_id><sourcerecordid>A418708221</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-a54560997dfac787d4294b5f1357a1dcc0773a7cd12d5cde2daf1d81285e214b3</originalsourceid><addsrcrecordid>eNqNkl2L1DAUhoso7jr6D0QLguDFjEmaNu2NsKxfAwsLft14Ec7kYyZD24xJRnb89Z463WUKClJKwjnPeXt4-2bZU0oWtBD09dbvQw_tYud7syCUMiboveycNgWbV4wU90_uZ9mjGLeElEVdVQ-zM4YXQgt2nn1_66w1wfTJQZsHE1Eumph7m6eNyQHr_sZpPPN4iMl0Qwc6k8Khx67OVes7-IU75Mm3JgzgClQywcHj7IGFNpon4znLvr5_9-Xy4_zq-sPy8uJqrqqGpTmUvKxI0whtQYlaaM4aviotLUoBVCtFhChAKE2ZLpU2TIOluqasLg2jfFXMsudH3V3roxx9iZJWjNYM3xqJ5ZHQHrZyF1wH4SA9OPmn4MNaQkhOtUYaKGpRE9JowrjQpFkJa3XJCVeVRdtQ6834tf2qM1qhdQHaiei007uNXPufkjPGOTo_y16MAsH_2JuY_rHySK0Bt3K99SimOheVvOC0FmTgkFr8hcJHm84p_CvWYX0y8GoygEwyN2kN-xjl8vOn_2evv03ZlyfsxkCbNtG3e4xPH6cgP4Iq-BiDsXfOUSKHYN-6IYdgyzHYOPbs1PW7odskF78BO3Pzog</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1621822188</pqid></control><display><type>article</type><title>Differential responses of the antioxidant system of ametryn and clomazone tolerant bacteria</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Peters, Leila Priscila ; Carvalho, Giselle ; Martins, Paula Fabiane ; Dourado, Manuella Nóbrega ; Vilhena, Milca Bartz ; Pileggi, Marcos ; Azevedo, Ricardo Antunes</creator><contributor>Lluch, Guillermo López</contributor><creatorcontrib>Peters, Leila Priscila ; Carvalho, Giselle ; Martins, Paula Fabiane ; Dourado, Manuella Nóbrega ; Vilhena, Milca Bartz ; Pileggi, Marcos ; Azevedo, Ricardo Antunes ; Lluch, Guillermo López</creatorcontrib><description>The herbicides ametryn and clomazone are widely used in sugarcane cultivation, and following microbial degradation are considered as soil and water contaminants. The exposure of microorganisms to pesticides can result in oxidative damage due to an increase in the production of reactive oxygen species (ROS). This study investigated the response of the antioxidant systems of two bacterial strains tolerant to the herbicides ametryn and clomazone. Bacteria were isolated from soil with a long history of ametryn and clomazone application. Comparative analyses based on 16S rRNA gene sequences revealed that strain CC07 is phylogenetically related to Pseudomonas aeruginosa and strain 4C07 to P. fulva. The two bacterial strains were grown for 14 h in the presence of separate and combined herbicides. Lipid peroxidation, reduced glutathione content (GSH) and antioxidant enzymes activities were evaluated. The overall results indicated that strain 4C07 formed an efficient mechanism to maintain the cellular redox balance by producing reactive oxygen species (ROS) and subsequently scavenging ROS in the presence of the herbicides. The growth of bacterium strain 4C07 was inhibited in the presence of clomazone alone, or in combination with ametryn, but increased glutathione reductase (GR) and glutathione S-transferase (GST) activities, and a higher GSH concentration were detected. Meanwhile, reduced superoxide dismutase (SOD), catalase (CAT) and GST activities and a lower concentration of GSH were detected in the bacterium strain CC07, which was able to achieve better growth in the presence of the herbicides. The results suggest that the two bacterial strains tolerate the ametryn and clomazone herbicides with distinctly different responses of the antioxidant systems.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0112271</identifier><identifier>PMID: 25380132</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agrochemicals ; Analysis ; Antioxidants ; Antioxidants (Nutrients) ; Antioxidants - metabolism ; Bacteria ; Biochemistry ; Biodegradation ; Biology and Life Sciences ; Catalase ; Catalase - metabolism ; Contaminants ; Cultivation ; Environmental degradation ; Enzymes ; Gene sequencing ; Glutathione ; Glutathione - metabolism ; Glutathione Peroxidase - metabolism ; Glutathione reductase ; Glutathione Reductase - metabolism ; Glutathione transferase ; Glutathione Transferase - metabolism ; Herbicides ; Herbicides - metabolism ; Hydrogen peroxide ; Isoxazoles - metabolism ; Lipid peroxidation ; Lipid Peroxidation - drug effects ; Metabolism ; Microbial degradation ; Microorganisms ; Oxazolidinones - metabolism ; Oxidative stress ; Oxidative Stress - drug effects ; Oxygen ; Peroxidation ; Pesticides ; Phylogeny ; Pseudomonas - drug effects ; Pseudomonas - enzymology ; Pseudomonas - genetics ; Pseudomonas - metabolism ; Pseudomonas aeruginosa ; Pseudomonas fulva ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; RNA ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sediment pollution ; Soil bacteria ; Soil contamination ; Soil degradation ; Soil Microbiology ; Soil Pollutants - metabolism ; Soil pollution ; Soil water ; Strains (organisms) ; Sugarcane ; Superoxide dismutase ; Superoxide Dismutase - metabolism ; Superoxides ; Triazines - metabolism ; Water pollution ; Weeds</subject><ispartof>PloS one, 2014-11, Vol.9 (11), p.e112271</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Peters et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Peters et al 2014 Peters et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a54560997dfac787d4294b5f1357a1dcc0773a7cd12d5cde2daf1d81285e214b3</citedby><cites>FETCH-LOGICAL-c692t-a54560997dfac787d4294b5f1357a1dcc0773a7cd12d5cde2daf1d81285e214b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4224425/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4224425/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25380132$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lluch, Guillermo López</contributor><creatorcontrib>Peters, Leila Priscila</creatorcontrib><creatorcontrib>Carvalho, Giselle</creatorcontrib><creatorcontrib>Martins, Paula Fabiane</creatorcontrib><creatorcontrib>Dourado, Manuella Nóbrega</creatorcontrib><creatorcontrib>Vilhena, Milca Bartz</creatorcontrib><creatorcontrib>Pileggi, Marcos</creatorcontrib><creatorcontrib>Azevedo, Ricardo Antunes</creatorcontrib><title>Differential responses of the antioxidant system of ametryn and clomazone tolerant bacteria</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The herbicides ametryn and clomazone are widely used in sugarcane cultivation, and following microbial degradation are considered as soil and water contaminants. The exposure of microorganisms to pesticides can result in oxidative damage due to an increase in the production of reactive oxygen species (ROS). This study investigated the response of the antioxidant systems of two bacterial strains tolerant to the herbicides ametryn and clomazone. Bacteria were isolated from soil with a long history of ametryn and clomazone application. Comparative analyses based on 16S rRNA gene sequences revealed that strain CC07 is phylogenetically related to Pseudomonas aeruginosa and strain 4C07 to P. fulva. The two bacterial strains were grown for 14 h in the presence of separate and combined herbicides. Lipid peroxidation, reduced glutathione content (GSH) and antioxidant enzymes activities were evaluated. The overall results indicated that strain 4C07 formed an efficient mechanism to maintain the cellular redox balance by producing reactive oxygen species (ROS) and subsequently scavenging ROS in the presence of the herbicides. The growth of bacterium strain 4C07 was inhibited in the presence of clomazone alone, or in combination with ametryn, but increased glutathione reductase (GR) and glutathione S-transferase (GST) activities, and a higher GSH concentration were detected. Meanwhile, reduced superoxide dismutase (SOD), catalase (CAT) and GST activities and a lower concentration of GSH were detected in the bacterium strain CC07, which was able to achieve better growth in the presence of the herbicides. The results suggest that the two bacterial strains tolerate the ametryn and clomazone herbicides with distinctly different responses of the antioxidant systems.</description><subject>Agrochemicals</subject><subject>Analysis</subject><subject>Antioxidants</subject><subject>Antioxidants (Nutrients)</subject><subject>Antioxidants - metabolism</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biodegradation</subject><subject>Biology and Life Sciences</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Contaminants</subject><subject>Cultivation</subject><subject>Environmental degradation</subject><subject>Enzymes</subject><subject>Gene sequencing</subject><subject>Glutathione</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Glutathione reductase</subject><subject>Glutathione Reductase - metabolism</subject><subject>Glutathione transferase</subject><subject>Glutathione Transferase - metabolism</subject><subject>Herbicides</subject><subject>Herbicides - metabolism</subject><subject>Hydrogen peroxide</subject><subject>Isoxazoles - metabolism</subject><subject>Lipid peroxidation</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Metabolism</subject><subject>Microbial degradation</subject><subject>Microorganisms</subject><subject>Oxazolidinones - metabolism</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Oxygen</subject><subject>Peroxidation</subject><subject>Pesticides</subject><subject>Phylogeny</subject><subject>Pseudomonas - drug effects</subject><subject>Pseudomonas - enzymology</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - metabolism</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas fulva</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Sediment pollution</subject><subject>Soil bacteria</subject><subject>Soil contamination</subject><subject>Soil degradation</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants - metabolism</subject><subject>Soil pollution</subject><subject>Soil water</subject><subject>Strains (organisms)</subject><subject>Sugarcane</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Superoxides</subject><subject>Triazines - metabolism</subject><subject>Water pollution</subject><subject>Weeds</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7jr6D0QLguDFjEmaNu2NsKxfAwsLft14Ec7kYyZD24xJRnb89Z463WUKClJKwjnPeXt4-2bZU0oWtBD09dbvQw_tYud7syCUMiboveycNgWbV4wU90_uZ9mjGLeElEVdVQ-zM4YXQgt2nn1_66w1wfTJQZsHE1Eumph7m6eNyQHr_sZpPPN4iMl0Qwc6k8Khx67OVes7-IU75Mm3JgzgClQywcHj7IGFNpon4znLvr5_9-Xy4_zq-sPy8uJqrqqGpTmUvKxI0whtQYlaaM4aviotLUoBVCtFhChAKE2ZLpU2TIOluqasLg2jfFXMsudH3V3roxx9iZJWjNYM3xqJ5ZHQHrZyF1wH4SA9OPmn4MNaQkhOtUYaKGpRE9JowrjQpFkJa3XJCVeVRdtQ6834tf2qM1qhdQHaiei007uNXPufkjPGOTo_y16MAsH_2JuY_rHySK0Bt3K99SimOheVvOC0FmTgkFr8hcJHm84p_CvWYX0y8GoygEwyN2kN-xjl8vOn_2evv03ZlyfsxkCbNtG3e4xPH6cgP4Iq-BiDsXfOUSKHYN-6IYdgyzHYOPbs1PW7odskF78BO3Pzog</recordid><startdate>20141107</startdate><enddate>20141107</enddate><creator>Peters, Leila Priscila</creator><creator>Carvalho, Giselle</creator><creator>Martins, Paula Fabiane</creator><creator>Dourado, Manuella Nóbrega</creator><creator>Vilhena, Milca Bartz</creator><creator>Pileggi, Marcos</creator><creator>Azevedo, Ricardo Antunes</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141107</creationdate><title>Differential responses of the antioxidant system of ametryn and clomazone tolerant bacteria</title><author>Peters, Leila Priscila ; Carvalho, Giselle ; Martins, Paula Fabiane ; Dourado, Manuella Nóbrega ; Vilhena, Milca Bartz ; Pileggi, Marcos ; Azevedo, Ricardo Antunes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-a54560997dfac787d4294b5f1357a1dcc0773a7cd12d5cde2daf1d81285e214b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Agrochemicals</topic><topic>Analysis</topic><topic>Antioxidants</topic><topic>Antioxidants (Nutrients)</topic><topic>Antioxidants - metabolism</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biodegradation</topic><topic>Biology and Life Sciences</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Contaminants</topic><topic>Cultivation</topic><topic>Environmental degradation</topic><topic>Enzymes</topic><topic>Gene sequencing</topic><topic>Glutathione</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Glutathione reductase</topic><topic>Glutathione Reductase - metabolism</topic><topic>Glutathione transferase</topic><topic>Glutathione Transferase - metabolism</topic><topic>Herbicides</topic><topic>Herbicides - metabolism</topic><topic>Hydrogen peroxide</topic><topic>Isoxazoles - metabolism</topic><topic>Lipid peroxidation</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Metabolism</topic><topic>Microbial degradation</topic><topic>Microorganisms</topic><topic>Oxazolidinones - metabolism</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Oxygen</topic><topic>Peroxidation</topic><topic>Pesticides</topic><topic>Phylogeny</topic><topic>Pseudomonas - drug effects</topic><topic>Pseudomonas - enzymology</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - metabolism</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas fulva</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Sediment pollution</topic><topic>Soil bacteria</topic><topic>Soil contamination</topic><topic>Soil degradation</topic><topic>Soil Microbiology</topic><topic>Soil Pollutants - metabolism</topic><topic>Soil pollution</topic><topic>Soil water</topic><topic>Strains (organisms)</topic><topic>Sugarcane</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Superoxides</topic><topic>Triazines - metabolism</topic><topic>Water pollution</topic><topic>Weeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peters, Leila Priscila</creatorcontrib><creatorcontrib>Carvalho, Giselle</creatorcontrib><creatorcontrib>Martins, Paula Fabiane</creatorcontrib><creatorcontrib>Dourado, Manuella Nóbrega</creatorcontrib><creatorcontrib>Vilhena, Milca Bartz</creatorcontrib><creatorcontrib>Pileggi, Marcos</creatorcontrib><creatorcontrib>Azevedo, Ricardo Antunes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peters, Leila Priscila</au><au>Carvalho, Giselle</au><au>Martins, Paula Fabiane</au><au>Dourado, Manuella Nóbrega</au><au>Vilhena, Milca Bartz</au><au>Pileggi, Marcos</au><au>Azevedo, Ricardo Antunes</au><au>Lluch, Guillermo López</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential responses of the antioxidant system of ametryn and clomazone tolerant bacteria</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-11-07</date><risdate>2014</risdate><volume>9</volume><issue>11</issue><spage>e112271</spage><pages>e112271-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The herbicides ametryn and clomazone are widely used in sugarcane cultivation, and following microbial degradation are considered as soil and water contaminants. The exposure of microorganisms to pesticides can result in oxidative damage due to an increase in the production of reactive oxygen species (ROS). This study investigated the response of the antioxidant systems of two bacterial strains tolerant to the herbicides ametryn and clomazone. Bacteria were isolated from soil with a long history of ametryn and clomazone application. Comparative analyses based on 16S rRNA gene sequences revealed that strain CC07 is phylogenetically related to Pseudomonas aeruginosa and strain 4C07 to P. fulva. The two bacterial strains were grown for 14 h in the presence of separate and combined herbicides. Lipid peroxidation, reduced glutathione content (GSH) and antioxidant enzymes activities were evaluated. The overall results indicated that strain 4C07 formed an efficient mechanism to maintain the cellular redox balance by producing reactive oxygen species (ROS) and subsequently scavenging ROS in the presence of the herbicides. The growth of bacterium strain 4C07 was inhibited in the presence of clomazone alone, or in combination with ametryn, but increased glutathione reductase (GR) and glutathione S-transferase (GST) activities, and a higher GSH concentration were detected. Meanwhile, reduced superoxide dismutase (SOD), catalase (CAT) and GST activities and a lower concentration of GSH were detected in the bacterium strain CC07, which was able to achieve better growth in the presence of the herbicides. The results suggest that the two bacterial strains tolerate the ametryn and clomazone herbicides with distinctly different responses of the antioxidant systems.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25380132</pmid><doi>10.1371/journal.pone.0112271</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-11, Vol.9 (11), p.e112271 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1621822188 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agrochemicals Analysis Antioxidants Antioxidants (Nutrients) Antioxidants - metabolism Bacteria Biochemistry Biodegradation Biology and Life Sciences Catalase Catalase - metabolism Contaminants Cultivation Environmental degradation Enzymes Gene sequencing Glutathione Glutathione - metabolism Glutathione Peroxidase - metabolism Glutathione reductase Glutathione Reductase - metabolism Glutathione transferase Glutathione Transferase - metabolism Herbicides Herbicides - metabolism Hydrogen peroxide Isoxazoles - metabolism Lipid peroxidation Lipid Peroxidation - drug effects Metabolism Microbial degradation Microorganisms Oxazolidinones - metabolism Oxidative stress Oxidative Stress - drug effects Oxygen Peroxidation Pesticides Phylogeny Pseudomonas - drug effects Pseudomonas - enzymology Pseudomonas - genetics Pseudomonas - metabolism Pseudomonas aeruginosa Pseudomonas fulva Reactive oxygen species Reactive Oxygen Species - metabolism RNA RNA, Ribosomal, 16S - genetics rRNA 16S Sediment pollution Soil bacteria Soil contamination Soil degradation Soil Microbiology Soil Pollutants - metabolism Soil pollution Soil water Strains (organisms) Sugarcane Superoxide dismutase Superoxide Dismutase - metabolism Superoxides Triazines - metabolism Water pollution Weeds |
| title | Differential responses of the antioxidant system of ametryn and clomazone tolerant bacteria |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T22%3A36%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20responses%20of%20the%20antioxidant%20system%20of%20ametryn%20and%20clomazone%20tolerant%20bacteria&rft.jtitle=PloS%20one&rft.au=Peters,%20Leila%20Priscila&rft.date=2014-11-07&rft.volume=9&rft.issue=11&rft.spage=e112271&rft.pages=e112271-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0112271&rft_dat=%3Cgale_plos_%3EA418708221%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1621822188&rft_id=info:pmid/25380132&rft_galeid=A418708221&rft_doaj_id=oai_doaj_org_article_ea3878009d0247d09b7ffd5404c6f538&rfr_iscdi=true |