Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the defence activity of Radix lagotis (Lymnaeidae) Haemocytes
Radix lagotis is an intermediate snail host of the nasal bird schistosome Trichobilharzia regenti. Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms an...
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| description | Radix lagotis is an intermediate snail host of the nasal bird schistosome Trichobilharzia regenti. Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms and determine the extent to which they are modulated by T. regenti. Histological observations of R. lagotis infected with T. regenti revealed that early phases of infection were accompanied by haemocyte accumulation around the developing larvae 2-36 h post exposure (p.e.) to the parasite. At later time points, 44-92 h p.e., no haemocytes were observed around T. regenti. Additionally, microtubular aggregates likely corresponding to phagocytosed ciliary plates of T. regenti miracidia were observed within haemocytes by use of transmission electron microscopy. When the infection was in the patent phase, haemocyte phagocytic activity and hydrogen peroxide production were significantly reduced in infected R. lagotis when compared to uninfected counterparts, whereas haemocyte abundance increased in infected snails. At a molecular level, protein kinase C (PKC) and extracellular-signal regulated kinase (ERK) were found to play an important role in regulating these defence reactions in R. lagotis. Moreover, haemocytes from snails with patent infection displayed lower PKC and ERK activity in cell adhesion assays when compared to those from uninfected snails, which may therefore be related to the reduced defence activities of these cells. These data provide the first integrated insight into the immunobiology of R. lagotis and demonstrate modulation of haemocyte-mediated responses in patent T. regenti infected snails. Given that immunomodulation occurs during patency, interference of snail-host defence by T. regenti might be important for the sustained production and/or release of infective cercariae. |
| doi_str_mv | 10.1371/journal.pone.0111696 |
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Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms and determine the extent to which they are modulated by T. regenti. Histological observations of R. lagotis infected with T. regenti revealed that early phases of infection were accompanied by haemocyte accumulation around the developing larvae 2-36 h post exposure (p.e.) to the parasite. At later time points, 44-92 h p.e., no haemocytes were observed around T. regenti. Additionally, microtubular aggregates likely corresponding to phagocytosed ciliary plates of T. regenti miracidia were observed within haemocytes by use of transmission electron microscopy. When the infection was in the patent phase, haemocyte phagocytic activity and hydrogen peroxide production were significantly reduced in infected R. lagotis when compared to uninfected counterparts, whereas haemocyte abundance increased in infected snails. At a molecular level, protein kinase C (PKC) and extracellular-signal regulated kinase (ERK) were found to play an important role in regulating these defence reactions in R. lagotis. Moreover, haemocytes from snails with patent infection displayed lower PKC and ERK activity in cell adhesion assays when compared to those from uninfected snails, which may therefore be related to the reduced defence activities of these cells. These data provide the first integrated insight into the immunobiology of R. lagotis and demonstrate modulation of haemocyte-mediated responses in patent T. regenti infected snails. Given that immunomodulation occurs during patency, interference of snail-host defence by T. regenti might be important for the sustained production and/or release of infective cercariae.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0111696</identifier><identifier>PMID: 25372492</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Biology and Life Sciences ; Biomphalaria glabrata ; Birds ; Cell adhesion ; Drinking water ; Echinostoma ; Electron microscopy ; Extracellular signal-regulated kinase ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Health aspects ; Hemocytes ; Hemocytes - immunology ; Hemocytes - metabolism ; Hemocytes - parasitology ; Host-Parasite Interactions - immunology ; Hydrogen ; Hydrogen peroxide ; Hydrogen Peroxide - metabolism ; Immunology ; Immunomodulation ; Infection ; Infections ; Kinases ; Laboratories ; Larvae ; Littorina littorea ; Lymnaea - immunology ; Lymnaea - metabolism ; Lymnaea - parasitology ; Lymnaea stagnalis ; Mollusks ; Parasites ; Parasitology ; Phagocytes ; Phagocytosis - immunology ; Protein kinase C ; Protein Kinase C - metabolism ; Proteins ; Radix lagotis ; Schistosoma mansoni ; Schistosomatidae - ultrastructure ; Science ; Signal transduction ; Snails ; Transmission electron microscopy ; Trichobilharzia regenti</subject><ispartof>PloS one, 2014-11, Vol.9 (11), p.e111696</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Skála et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Skála et al 2014 Skála et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-2b484024cc6b4ce6824695aac774802062185943da9f5acf5ca282ec8ba342593</citedby><cites>FETCH-LOGICAL-c557t-2b484024cc6b4ce6824695aac774802062185943da9f5acf5ca282ec8ba342593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221104/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221104/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23853,27911,27912,53778,53780,79355,79356</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25372492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Doucet, Daniel</contributor><creatorcontrib>Skála, Vladimír</creatorcontrib><creatorcontrib>Černíková, Alena</creatorcontrib><creatorcontrib>Jindrová, Zuzana</creatorcontrib><creatorcontrib>Kašný, Martin</creatorcontrib><creatorcontrib>Vostrý, Martin</creatorcontrib><creatorcontrib>Walker, Anthony J</creatorcontrib><creatorcontrib>Horák, Petr</creatorcontrib><title>Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the defence activity of Radix lagotis (Lymnaeidae) Haemocytes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Radix lagotis is an intermediate snail host of the nasal bird schistosome Trichobilharzia regenti. Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms and determine the extent to which they are modulated by T. regenti. Histological observations of R. lagotis infected with T. regenti revealed that early phases of infection were accompanied by haemocyte accumulation around the developing larvae 2-36 h post exposure (p.e.) to the parasite. At later time points, 44-92 h p.e., no haemocytes were observed around T. regenti. Additionally, microtubular aggregates likely corresponding to phagocytosed ciliary plates of T. regenti miracidia were observed within haemocytes by use of transmission electron microscopy. When the infection was in the patent phase, haemocyte phagocytic activity and hydrogen peroxide production were significantly reduced in infected R. lagotis when compared to uninfected counterparts, whereas haemocyte abundance increased in infected snails. At a molecular level, protein kinase C (PKC) and extracellular-signal regulated kinase (ERK) were found to play an important role in regulating these defence reactions in R. lagotis. Moreover, haemocytes from snails with patent infection displayed lower PKC and ERK activity in cell adhesion assays when compared to those from uninfected snails, which may therefore be related to the reduced defence activities of these cells. These data provide the first integrated insight into the immunobiology of R. lagotis and demonstrate modulation of haemocyte-mediated responses in patent T. regenti infected snails. Given that immunomodulation occurs during patency, interference of snail-host defence by T. regenti might be important for the sustained production and/or release of infective cercariae.</description><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Biomphalaria glabrata</subject><subject>Birds</subject><subject>Cell adhesion</subject><subject>Drinking water</subject><subject>Echinostoma</subject><subject>Electron microscopy</subject><subject>Extracellular signal-regulated kinase</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Health aspects</subject><subject>Hemocytes</subject><subject>Hemocytes - immunology</subject><subject>Hemocytes - metabolism</subject><subject>Hemocytes - parasitology</subject><subject>Host-Parasite Interactions - immunology</subject><subject>Hydrogen</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Immunology</subject><subject>Immunomodulation</subject><subject>Infection</subject><subject>Infections</subject><subject>Kinases</subject><subject>Laboratories</subject><subject>Larvae</subject><subject>Littorina littorea</subject><subject>Lymnaea - immunology</subject><subject>Lymnaea - metabolism</subject><subject>Lymnaea - parasitology</subject><subject>Lymnaea stagnalis</subject><subject>Mollusks</subject><subject>Parasites</subject><subject>Parasitology</subject><subject>Phagocytes</subject><subject>Phagocytosis - immunology</subject><subject>Protein kinase C</subject><subject>Protein Kinase C - metabolism</subject><subject>Proteins</subject><subject>Radix lagotis</subject><subject>Schistosoma mansoni</subject><subject>Schistosomatidae - ultrastructure</subject><subject>Science</subject><subject>Signal transduction</subject><subject>Snails</subject><subject>Transmission electron microscopy</subject><subject>Trichobilharzia regenti</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNp1kk9v1DAQxSMEoqXwDRBEQkLtYRf_d8wBqSqUrrQSEpSzNXGcxKtsvNhOxXLjm5Nt0qo9cPLIfvOb8dPLstcYLTGV-MPGD6GHbrnzvV0ijLFQ4kl2jBUlC0EQffqgPspexLhBiNNCiOfZEeFUEqbIcfZ31dfdYHtjc1_n18GZ1peuayH8cZAH29g-ufz0sxsLCx_zH6Z1Mfnot5BcBfYs932eWptXtr6lgEnuxqX9AfcdKvc776DxycX8dL3f9mCnriuwW2_2ycaX2bMaumhfzedJ9vPyy_XF1WL97evq4ny9MJzLtCAlKxgizBhRMmNFQZhQHMBIyQpEkCC44IrRClTNwdTcACmINUUJlBGu6En2duLuOh_17F7UePSHK44LMipWk6LysNG74LYQ9tqD07cXPjQaQnKms1qUkgKlhtRVyShWyhCpsJAIqCgQNyPr0zxtKLe2MqONAbpH0McvvWt14280IwRjxEbAuxkQ_K_BxvSfld9PqgbGrVoLXWqj74bkfB_1OcOFRJJzMQrZJDTBxxhsfb8KRvqQpzu8PuRJz3ka2948_MZ9012A6D_Ttsj8</recordid><startdate>20141105</startdate><enddate>20141105</enddate><creator>Skála, Vladimír</creator><creator>Černíková, Alena</creator><creator>Jindrová, Zuzana</creator><creator>Kašný, Martin</creator><creator>Vostrý, Martin</creator><creator>Walker, Anthony J</creator><creator>Horák, Petr</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141105</creationdate><title>Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the defence activity of Radix lagotis (Lymnaeidae) Haemocytes</title><author>Skála, Vladimír ; Černíková, Alena ; Jindrová, Zuzana ; Kašný, Martin ; Vostrý, Martin ; Walker, Anthony J ; Horák, Petr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-2b484024cc6b4ce6824695aac774802062185943da9f5acf5ca282ec8ba342593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Biology and Life Sciences</topic><topic>Biomphalaria glabrata</topic><topic>Birds</topic><topic>Cell adhesion</topic><topic>Drinking water</topic><topic>Echinostoma</topic><topic>Electron microscopy</topic><topic>Extracellular signal-regulated kinase</topic><topic>Extracellular Signal-Regulated MAP Kinases - 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Changes in defence responses in infected snails that might be related to host-parasite compatibility are not known. This study therefore aimed to characterize R. lagotis haemocyte defence mechanisms and determine the extent to which they are modulated by T. regenti. Histological observations of R. lagotis infected with T. regenti revealed that early phases of infection were accompanied by haemocyte accumulation around the developing larvae 2-36 h post exposure (p.e.) to the parasite. At later time points, 44-92 h p.e., no haemocytes were observed around T. regenti. Additionally, microtubular aggregates likely corresponding to phagocytosed ciliary plates of T. regenti miracidia were observed within haemocytes by use of transmission electron microscopy. When the infection was in the patent phase, haemocyte phagocytic activity and hydrogen peroxide production were significantly reduced in infected R. lagotis when compared to uninfected counterparts, whereas haemocyte abundance increased in infected snails. At a molecular level, protein kinase C (PKC) and extracellular-signal regulated kinase (ERK) were found to play an important role in regulating these defence reactions in R. lagotis. Moreover, haemocytes from snails with patent infection displayed lower PKC and ERK activity in cell adhesion assays when compared to those from uninfected snails, which may therefore be related to the reduced defence activities of these cells. These data provide the first integrated insight into the immunobiology of R. lagotis and demonstrate modulation of haemocyte-mediated responses in patent T. regenti infected snails. Given that immunomodulation occurs during patency, interference of snail-host defence by T. regenti might be important for the sustained production and/or release of infective cercariae.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25372492</pmid><doi>10.1371/journal.pone.0111696</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Biology and Life Sciences Biomphalaria glabrata Birds Cell adhesion Drinking water Echinostoma Electron microscopy Extracellular signal-regulated kinase Extracellular Signal-Regulated MAP Kinases - metabolism Health aspects Hemocytes Hemocytes - immunology Hemocytes - metabolism Hemocytes - parasitology Host-Parasite Interactions - immunology Hydrogen Hydrogen peroxide Hydrogen Peroxide - metabolism Immunology Immunomodulation Infection Infections Kinases Laboratories Larvae Littorina littorea Lymnaea - immunology Lymnaea - metabolism Lymnaea - parasitology Lymnaea stagnalis Mollusks Parasites Parasitology Phagocytes Phagocytosis - immunology Protein kinase C Protein Kinase C - metabolism Proteins Radix lagotis Schistosoma mansoni Schistosomatidae - ultrastructure Science Signal transduction Snails Transmission electron microscopy Trichobilharzia regenti |
| title | Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the defence activity of Radix lagotis (Lymnaeidae) Haemocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T00%3A35%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20Trichobilharzia%20regenti%20(Digenea:%20Schistosomatidae)%20on%20the%20defence%20activity%20of%20Radix%20lagotis%20(Lymnaeidae)%20Haemocytes&rft.jtitle=PloS%20one&rft.au=Sk%C3%A1la,%20Vladim%C3%ADr&rft.date=2014-11-05&rft.volume=9&rft.issue=11&rft.spage=e111696&rft.pages=e111696-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0111696&rft_dat=%3Cgale_plos_%3EA418707556%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1620595182&rft_id=info:pmid/25372492&rft_galeid=A418707556&rft_doaj_id=oai_doaj_org_article_6b73a33c2fdb43199c2791670a36805c&rfr_iscdi=true |