Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China
Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period...
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| creator | Zhang, Lei Ye, Ling Ojcius, David M Lou, Xiuyu Wang, Chengwei feng, Cen Sun, Yi Wang, Zhongfa Li, Shibo Zhang, Yanjun |
| description | Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes. |
| doi_str_mv | 10.1371/journal.pone.0111127 |
| format | Article |
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A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0111127</identifier><identifier>PMID: 25356556</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Amino Acid Sequence ; Analysis ; Antibodies ; Antigenic determinants ; Bunyaviridae Infections - epidemiology ; Bunyaviridae Infections - virology ; Cell culture ; China - epidemiology ; Clusters ; Disease control ; Disease prevention ; Epidemiological Monitoring ; Epidemiology ; Epitopes ; Epitopes, B-Lymphocyte - chemistry ; Epitopes, B-Lymphocyte - immunology ; Fatalities ; Fever ; Fever - complications ; Fever - epidemiology ; Fever - virology ; Genetic diversity ; Genotypes ; Health aspects ; Homology ; Humans ; Immunoglobulins ; Infection ; Infections ; Lymphocytes B ; Medicine and Health Sciences ; Middle Aged ; Molecular Sequence Data ; Mutation ; Nucleocapsid Proteins - genetics ; Nucleocapsids ; Outbreaks ; Phlebovirus - genetics ; Phylogenetics ; Phylogeny ; Proteins ; RNA polymerase ; Rural areas ; Rural Population - statistics & numerical data ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Seroepidemiologic Studies ; Strains (organisms) ; Studies ; Surveillance ; Syndrome ; Thrombocytopenia ; Thrombocytopenia - complications ; Thrombocytopenia - epidemiology ; Thrombocytopenia - virology ; Viruses ; Zhang, Ling</subject><ispartof>PloS one, 2014-10, Vol.9 (10), p.e111127</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Zhang et al 2014 Zhang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-d9abf728f38987b3483ac15a53954cbae300aa452d100ca01b880da498aa2e8d3</citedby><cites>FETCH-LOGICAL-c692t-d9abf728f38987b3483ac15a53954cbae300aa452d100ca01b880da498aa2e8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214719/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214719/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,2914,23846,27903,27904,53770,53772,79347,79348</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25356556$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yu, Xue-jie</contributor><creatorcontrib>Zhang, Lei</creatorcontrib><creatorcontrib>Ye, Ling</creatorcontrib><creatorcontrib>Ojcius, David M</creatorcontrib><creatorcontrib>Lou, Xiuyu</creatorcontrib><creatorcontrib>Wang, Chengwei</creatorcontrib><creatorcontrib>feng, Cen</creatorcontrib><creatorcontrib>Sun, Yi</creatorcontrib><creatorcontrib>Wang, Zhongfa</creatorcontrib><creatorcontrib>Li, Shibo</creatorcontrib><creatorcontrib>Zhang, Yanjun</creatorcontrib><title>Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Severe fever with thrombocytopenia syndrome virus (SFTSV) infections have recently been found in rural regions of Zhejiang. A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amino Acid Sequence</subject><subject>Analysis</subject><subject>Antibodies</subject><subject>Antigenic determinants</subject><subject>Bunyaviridae Infections - epidemiology</subject><subject>Bunyaviridae Infections - virology</subject><subject>Cell culture</subject><subject>China - epidemiology</subject><subject>Clusters</subject><subject>Disease control</subject><subject>Disease prevention</subject><subject>Epidemiological Monitoring</subject><subject>Epidemiology</subject><subject>Epitopes</subject><subject>Epitopes, B-Lymphocyte - chemistry</subject><subject>Epitopes, B-Lymphocyte - immunology</subject><subject>Fatalities</subject><subject>Fever</subject><subject>Fever - complications</subject><subject>Fever - epidemiology</subject><subject>Fever - virology</subject><subject>Genetic diversity</subject><subject>Genotypes</subject><subject>Health aspects</subject><subject>Homology</subject><subject>Humans</subject><subject>Immunoglobulins</subject><subject>Infection</subject><subject>Infections</subject><subject>Lymphocytes B</subject><subject>Medicine and Health Sciences</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nucleocapsid Proteins - genetics</subject><subject>Nucleocapsids</subject><subject>Outbreaks</subject><subject>Phlebovirus - genetics</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>RNA polymerase</subject><subject>Rural areas</subject><subject>Rural Population - statistics & numerical data</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Seroepidemiologic Studies</subject><subject>Strains (organisms)</subject><subject>Studies</subject><subject>Surveillance</subject><subject>Syndrome</subject><subject>Thrombocytopenia</subject><subject>Thrombocytopenia - complications</subject><subject>Thrombocytopenia - epidemiology</subject><subject>Thrombocytopenia - virology</subject><subject>Viruses</subject><subject>Zhang, Ling</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7jr6D0QLgiA4Y9I0bXojLIMfAwsLfl14E07T0zZDp5lN0tXx15s63WUKCrYXCSfPe3ry9o2ip5SsKMvpm60ZbA_dam96XBEaniS_F53TgiXLLCHs_sn-LHrk3JYQzkSWPYzOEs54xnl2Hql1CxaUR6t_gdemj00dO7xBi3E9LvEP7dvYt9bsSqMO3uyx1xC7Q1-FEsa6j-1goYstNkHuRv33Frca-uZ1vG51D4-jBzV0Dp9M6yL6-v7dl_XH5eXVh8364nKpsiLxy6qAss4TUTNRiLxkqWCgKAfOCp6qEpARApDypKKEKCC0FIJUkBYCIEFRsUX0_Nh33xknJ3-cpBkVYrxvHojNkagMbOXe6h3YgzSg5Z-CsY0E67XqUNY5LwqeCMSUpVmuCoVhCJoKgZDxYuz1dvraUO6wUtj7YMOs6fyk161szI1ME5rm4dcsohdTA2uuB3T-HyNPVANhKt3XJjRTO-2UvEhpTlNOKAvU6i9UeCvcaRUSUutQnwlezQSB8fjTNzA4JzefP_0_e_Vtzr48YVuEzrfOdMMYLTcH0yOorHHOYn3nHCVyDPitG3IMuJwCHmTPTl2_E90mmv0Gmpb2YA</recordid><startdate>20141030</startdate><enddate>20141030</enddate><creator>Zhang, Lei</creator><creator>Ye, Ling</creator><creator>Ojcius, David M</creator><creator>Lou, Xiuyu</creator><creator>Wang, Chengwei</creator><creator>feng, Cen</creator><creator>Sun, Yi</creator><creator>Wang, Zhongfa</creator><creator>Li, Shibo</creator><creator>Zhang, Yanjun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141030</creationdate><title>Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China</title><author>Zhang, Lei ; Ye, Ling ; Ojcius, David M ; Lou, Xiuyu ; Wang, Chengwei ; feng, Cen ; Sun, Yi ; Wang, Zhongfa ; Li, Shibo ; Zhang, Yanjun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-d9abf728f38987b3483ac15a53954cbae300aa452d100ca01b880da498aa2e8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Amino Acid Sequence</topic><topic>Analysis</topic><topic>Antibodies</topic><topic>Antigenic determinants</topic><topic>Bunyaviridae Infections - 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A severe fever with thrombocytopenia syndrome (SFTS) surveillance and sero-epidemiological investigation was conducted in the districts with outbreaks. During the study period of 2011-2014, a total of 51 SFTSV infection cases were identified and the case fatality rate was 12% (6/51). Ninety two percent of the patients (47/51) were over 50 years of age, and 63% (32/51) of laboratory confirmed cases occurred from May to July. Nine percent (11/120) of the serum samples from local healthy people without symptoms were found to be positive for antibodies to the SFTS virus. SFTSV strains were isolated by culture using Vero, and the whole genomic sequences of two SFTSV strains (01 and Zhao) were sequenced and submitted to the GenBank. Homology analysis showed that the similarity of the target nucleocapsid gene from the SFTSV strains from different geographic areas was 94.2-100%. From the constructed phylogenetic tree, it was found that all the SFTSV strains diverged into two main clusters. Only the SFTSV strains from the Zhejiang (Daishan) region of China and the Yamaguchi, Miyazakj regions of Japan, were clustered into lineage II, consistent with both of these regions being isolated areas with similar geographic features. Two out of eight predicted linear B cell epitopes from the nucleocapsid protein showed mutations between the SFTSV strains of different clusters, but did not contribute to the binding ability of the specific SFTSV antibodies. This study confirmed that SFTSV has been circulating naturally and can cause a seasonal prevalence in Daishan, China. The results also suggest that the molecular characteristics of SFTSV are associated with the geographic region and all SFTSV strains can be divided into two genotypes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25356556</pmid><doi>10.1371/journal.pone.0111127</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-10, Vol.9 (10), p.e111127 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1618835657 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adult Aged Aged, 80 and over Amino Acid Sequence Analysis Antibodies Antigenic determinants Bunyaviridae Infections - epidemiology Bunyaviridae Infections - virology Cell culture China - epidemiology Clusters Disease control Disease prevention Epidemiological Monitoring Epidemiology Epitopes Epitopes, B-Lymphocyte - chemistry Epitopes, B-Lymphocyte - immunology Fatalities Fever Fever - complications Fever - epidemiology Fever - virology Genetic diversity Genotypes Health aspects Homology Humans Immunoglobulins Infection Infections Lymphocytes B Medicine and Health Sciences Middle Aged Molecular Sequence Data Mutation Nucleocapsid Proteins - genetics Nucleocapsids Outbreaks Phlebovirus - genetics Phylogenetics Phylogeny Proteins RNA polymerase Rural areas Rural Population - statistics & numerical data Sequence Analysis, DNA Sequence Homology, Nucleic Acid Seroepidemiologic Studies Strains (organisms) Studies Surveillance Syndrome Thrombocytopenia Thrombocytopenia - complications Thrombocytopenia - epidemiology Thrombocytopenia - virology Viruses Zhang, Ling |
| title | Characterization of severe fever with thrombocytopenia syndrome in rural regions of Zhejiang, China |
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