Transcriptome-wide mapping of pseudouridines: pseudouridine synthases modify specific mRNAs in S. cerevisiae

We developed a novel technique, called pseudouridine site identification sequencing (PSI-seq), for the transcriptome-wide mapping of pseudouridylation sites with single-base resolution from cellular RNAs based on the induced termination of reverse transcription specifically at pseudouridines followi...

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Veröffentlicht in:	PloS one 2014-10, Vol.9 (10), p.e110799-e110799
Hauptverfasser:	Lovejoy, Alexander F, Riordan, Daniel P, Brown, Patrick O
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Baking yeast Biochemistry Biology and life sciences Gene expression Gene Expression Profiling - methods Intramolecular Transferases - genetics Intramolecular Transferases - metabolism Mapping Medicine Pseudouridine - analysis Pseudouridine synthase Pseudouridylation Research and Analysis Methods Reverse transcription Ribonucleic acid RNA RNA modification RNA Processing, Post-Transcriptional RNA, Fungal - chemistry RNA, Fungal - metabolism RNA, Messenger - chemistry RNA, Messenger - metabolism RNA, Ribosomal - chemistry RNA, Ribosomal - metabolism rRNA 18S rRNA 25S Saccharomyces cerevisiae Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Sequence Analysis, RNA - methods Transcription termination Yeast
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