Comparative evaluation of methods for estimating retinal ganglion cell loss in retinal sections and wholemounts

To investigate the reliability of different methods of quantifying retinal ganglion cells (RGCs) in rat retinal sections and wholemounts from eyes with either intact optic nerves or those axotomised after optic nerve crush (ONC). Adult rats received a unilateral ONC and after 21 days the numbers of...

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Veröffentlicht in:	PloS one 2014-10, Vol.9 (10), p.e110612-e110612
Hauptverfasser:	Mead, Ben, Thompson, Adam, Scheven, Ben A, Logan, Ann, Berry, Martin, Leadbeater, Wendy
Format:	Artikel
Sprache:	eng
Schlagworte:	Albinism Amacrine cells Animals Astrocytes Biology and Life Sciences Brn-3 protein Cell Count Cell Culture Techniques - methods Cell Death Comparative analysis Dentistry Diagnosis Eye Eye (anatomy) Female Ganglion cysts Gene expression Glaucoma Harvesting Hypertension Image detection Islet-1 protein LIM-Homeodomain Proteins - metabolism Macrophages Medicine Medicine and Health Sciences Methods Microglia Microscopy - methods Nerves Neurobiology Neurosciences Optic nerve Rats Rats, Sprague-Dawley Research and Analysis Methods Retina Retinal ganglion cells Retinal Ganglion Cells - cytology Rodents Staining and Labeling Transcription Factor Brn-3A - metabolism Transcription Factors - metabolism Tubulin Tubulin - metabolism
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