Gene expression pattern after insertion of dexamethasone-eluting electrode into the guinea pig cochlea
A cochlear implant is an indispensable apparatus for a profound hearing loss patient. But insertion of the electrode entails a great deal of stress to the cochlea, and may cause irreversible damage to hair cells and related nerve structure. Although damage prevention effects of dexamethasone have be...
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| creator | Takumi, Yutaka Nishio, Shin-ya Mugridge, Kenneth Oguchi, Tomohiro Hashimoto, Shigenari Suzuki, Nobuyoshi Iwasaki, Satoshi Jolly, Claude Usami, Shin-ichi |
| description | A cochlear implant is an indispensable apparatus for a profound hearing loss patient. But insertion of the electrode entails a great deal of stress to the cochlea, and may cause irreversible damage to hair cells and related nerve structure. Although damage prevention effects of dexamethasone have been reported, long-term administration is difficult. In this study, we used a dexamethasone-eluting electrode in the guinea pig cochlea, and compared the gene expression after 7 days insertion with that of a normal electrode and non-surgically treated control by microarray. 40 genes were up-regulated 2-fold or more in the normal electrode group compared to the non-surgically treated group. Most of the up-regulated genes were associated with immune response and inflammation. In the dexamethasone-eluting group, compared to the normal electrode group, 7 of the 40 genes were further up-regulated, while 12 of them were down-regulated and there was a tendency to return to the non-surgical condition. 9 genes were down-regulated 2-fold or less with normal electrode insertion, and 4 of the 9 tended to return to the non-surgical condition in the dexamethasone-eluting group. These genes are certainly involved in the maintenance of the physiological functions of the cochlea. Our results indicate that the dexamethasone-eluting electrode will have an effect on the normalization of homeostasis in the cochlea. |
| doi_str_mv | 10.1371/journal.pone.0110238 |
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But insertion of the electrode entails a great deal of stress to the cochlea, and may cause irreversible damage to hair cells and related nerve structure. Although damage prevention effects of dexamethasone have been reported, long-term administration is difficult. In this study, we used a dexamethasone-eluting electrode in the guinea pig cochlea, and compared the gene expression after 7 days insertion with that of a normal electrode and non-surgically treated control by microarray. 40 genes were up-regulated 2-fold or more in the normal electrode group compared to the non-surgically treated group. Most of the up-regulated genes were associated with immune response and inflammation. In the dexamethasone-eluting group, compared to the normal electrode group, 7 of the 40 genes were further up-regulated, while 12 of them were down-regulated and there was a tendency to return to the non-surgical condition. 9 genes were down-regulated 2-fold or less with normal electrode insertion, and 4 of the 9 tended to return to the non-surgical condition in the dexamethasone-eluting group. These genes are certainly involved in the maintenance of the physiological functions of the cochlea. Our results indicate that the dexamethasone-eluting electrode will have an effect on the normalization of homeostasis in the cochlea.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0110238</identifier><identifier>PMID: 25329543</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Central nervous system depressants ; Chemokines ; Cochlea ; Cochlea - drug effects ; Cochlea - metabolism ; Cochlea - surgery ; Cochlear Implantation - adverse effects ; Cochlear Implantation - instrumentation ; Cochlear implants ; Comparative analysis ; Cytokines ; Damage prevention ; Deafness ; Dexamethasone ; Dexamethasone - pharmacology ; DNA microarrays ; Electrodes ; Electrodes - adverse effects ; Gene expression ; Genes ; Guinea Pigs ; Hair cells ; Hearing loss ; Homeostasis ; Immune response ; Immune system ; Insertion ; Male ; Medicine ; Medicine and Health Sciences ; Otolaryngology ; Proteins ; Steroids ; Surgery ; Transcriptome - drug effects ; Transplants & implants ; Trauma</subject><ispartof>PloS one, 2014-10, Vol.9 (10), p.e110238</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Takumi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Takumi et al 2014 Takumi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-3a7bee900b4eb8bb5ee551bc42d66c02a7364241c654a563a566e0b43075de1c3</citedby><cites>FETCH-LOGICAL-c692t-3a7bee900b4eb8bb5ee551bc42d66c02a7364241c654a563a566e0b43075de1c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203767/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4203767/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25329543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zeng, Fan-Gang</contributor><creatorcontrib>Takumi, Yutaka</creatorcontrib><creatorcontrib>Nishio, Shin-ya</creatorcontrib><creatorcontrib>Mugridge, Kenneth</creatorcontrib><creatorcontrib>Oguchi, Tomohiro</creatorcontrib><creatorcontrib>Hashimoto, Shigenari</creatorcontrib><creatorcontrib>Suzuki, Nobuyoshi</creatorcontrib><creatorcontrib>Iwasaki, Satoshi</creatorcontrib><creatorcontrib>Jolly, Claude</creatorcontrib><creatorcontrib>Usami, Shin-ichi</creatorcontrib><title>Gene expression pattern after insertion of dexamethasone-eluting electrode into the guinea pig cochlea</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A cochlear implant is an indispensable apparatus for a profound hearing loss patient. But insertion of the electrode entails a great deal of stress to the cochlea, and may cause irreversible damage to hair cells and related nerve structure. Although damage prevention effects of dexamethasone have been reported, long-term administration is difficult. In this study, we used a dexamethasone-eluting electrode in the guinea pig cochlea, and compared the gene expression after 7 days insertion with that of a normal electrode and non-surgically treated control by microarray. 40 genes were up-regulated 2-fold or more in the normal electrode group compared to the non-surgically treated group. Most of the up-regulated genes were associated with immune response and inflammation. In the dexamethasone-eluting group, compared to the normal electrode group, 7 of the 40 genes were further up-regulated, while 12 of them were down-regulated and there was a tendency to return to the non-surgical condition. 9 genes were down-regulated 2-fold or less with normal electrode insertion, and 4 of the 9 tended to return to the non-surgical condition in the dexamethasone-eluting group. These genes are certainly involved in the maintenance of the physiological functions of the cochlea. Our results indicate that the dexamethasone-eluting electrode will have an effect on the normalization of homeostasis in the cochlea.</description><subject>Animals</subject><subject>Central nervous system depressants</subject><subject>Chemokines</subject><subject>Cochlea</subject><subject>Cochlea - drug effects</subject><subject>Cochlea - metabolism</subject><subject>Cochlea - surgery</subject><subject>Cochlear Implantation - adverse effects</subject><subject>Cochlear Implantation - instrumentation</subject><subject>Cochlear implants</subject><subject>Comparative analysis</subject><subject>Cytokines</subject><subject>Damage prevention</subject><subject>Deafness</subject><subject>Dexamethasone</subject><subject>Dexamethasone - pharmacology</subject><subject>DNA microarrays</subject><subject>Electrodes</subject><subject>Electrodes - adverse effects</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Guinea Pigs</subject><subject>Hair cells</subject><subject>Hearing loss</subject><subject>Homeostasis</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Insertion</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Otolaryngology</subject><subject>Proteins</subject><subject>Steroids</subject><subject>Surgery</subject><subject>Transcriptome - 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But insertion of the electrode entails a great deal of stress to the cochlea, and may cause irreversible damage to hair cells and related nerve structure. Although damage prevention effects of dexamethasone have been reported, long-term administration is difficult. In this study, we used a dexamethasone-eluting electrode in the guinea pig cochlea, and compared the gene expression after 7 days insertion with that of a normal electrode and non-surgically treated control by microarray. 40 genes were up-regulated 2-fold or more in the normal electrode group compared to the non-surgically treated group. Most of the up-regulated genes were associated with immune response and inflammation. In the dexamethasone-eluting group, compared to the normal electrode group, 7 of the 40 genes were further up-regulated, while 12 of them were down-regulated and there was a tendency to return to the non-surgical condition. 9 genes were down-regulated 2-fold or less with normal electrode insertion, and 4 of the 9 tended to return to the non-surgical condition in the dexamethasone-eluting group. These genes are certainly involved in the maintenance of the physiological functions of the cochlea. Our results indicate that the dexamethasone-eluting electrode will have an effect on the normalization of homeostasis in the cochlea.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25329543</pmid><doi>10.1371/journal.pone.0110238</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Central nervous system depressants Chemokines Cochlea Cochlea - drug effects Cochlea - metabolism Cochlea - surgery Cochlear Implantation - adverse effects Cochlear Implantation - instrumentation Cochlear implants Comparative analysis Cytokines Damage prevention Deafness Dexamethasone Dexamethasone - pharmacology DNA microarrays Electrodes Electrodes - adverse effects Gene expression Genes Guinea Pigs Hair cells Hearing loss Homeostasis Immune response Immune system Insertion Male Medicine Medicine and Health Sciences Otolaryngology Proteins Steroids Surgery Transcriptome - drug effects Transplants & implants Trauma |
| title | Gene expression pattern after insertion of dexamethasone-eluting electrode into the guinea pig cochlea |
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