iBrick: a new standard for iterative assembly of biological parts with homing endonucleases

The BioBricks standard has made the construction of DNA modules easier, quicker and cheaper. So far, over 100 BioBricks assembly schemes have been developed and many of them, including the original standard of BBF RFC 10, are now widely used. However, because the restriction endonucleases employed b...

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Veröffentlicht in:	PloS one 2014-10, Vol.9 (10), p.e110852-e110852
Hauptverfasser:	Liu, Jia-Kun, Chen, Wei-Hua, Ren, Shuang-Xi, Zhao, Guo-Ping, Wang, Jin
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinorhodin Anthraquinones Antibiotics Assembly Biology and Life Sciences Cloning Cloning, Molecular - methods Clusters Construction Construction standards Deoxyribonucleic acid DNA DNA sequencing DNA, Bacterial - genetics E coli Endonucleases - chemistry Escherichia coli Escherichia coli - genetics Gene sequencing Genes Genes, Bacterial Genetic engineering Genomes Genomics Homing Laboratories Multigene Family Nomenclature Nucleotide sequence Nucleotide sequencing Physiology Plasmids Proteins Research parks Streptomyces Streptomyces - genetics Synthetic biology Vectors (Biology)
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