Genotypes and transmitted drug resistance among treatment-naive HIV-1-infected patients in a northwestern province, China: trends from 2003 to 2013
Transmitted drug resistance (TDR) reduces the efficacy of initial antiretroviral treatment and has become a public health concern. Little information is available regarding the genetic diversity of HIV-1 and the prevalence of TDR among treatment-naïve patients in a northwestern province of China sin...
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| Veröffentlicht in: | PloS one 2014-10, Vol.9 (10), p.e109821-e109821 |
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| creator | Zhao, Ke Kang, Wenzhen Liu, Qingquan Li, Yuan Liu, Qing Jiang, Wei Zhuang, Yan Guo, Zisheng Yu, Zhuoran Li, Xinhong Wang, Chunfu Yao, Na Sun, Yongtao |
| description | Transmitted drug resistance (TDR) reduces the efficacy of initial antiretroviral treatment and has become a public health concern. Little information is available regarding the genetic diversity of HIV-1 and the prevalence of TDR among treatment-naïve patients in a northwestern province of China since the implementation of national free antiretroviral therapy (ART).
Blood samples from 372 HIV-1 treatment-naive patients were collected between 2003 and 2013 in Shaanxi province. Viral RNA was extracted for nested PCR, and phylogenetic reconstruction and recombination analyses were performed to characterize patterns of the HIV-1 subtypes. Genotypic drug resistance testing was performed using an in-house assay to determine trends in the prevalence of HIV-1 transmitted drug resistance.
Multiple genotypes were identified among the patients in Shaanxi, including B (25.0%), C (0.3%), G (0.3%), and CRF01_AE (39.2%), CRF07_BC (32.7%), CRF08_BC (0.8%), CRF55_01B (1.1%), and URFs (0.6%). The subtypes were associated with the transmission routes (χ2 = 77.113, p < 0.01). In this study, a low baseline CD4+ T cell count and a high viral load were found among CRF01_AE-infected patients compared with patients who were infected with non-CRF01_AE (p |
| doi_str_mv | 10.1371/journal.pone.0109821 |
| format | Article |
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Blood samples from 372 HIV-1 treatment-naive patients were collected between 2003 and 2013 in Shaanxi province. Viral RNA was extracted for nested PCR, and phylogenetic reconstruction and recombination analyses were performed to characterize patterns of the HIV-1 subtypes. Genotypic drug resistance testing was performed using an in-house assay to determine trends in the prevalence of HIV-1 transmitted drug resistance.
Multiple genotypes were identified among the patients in Shaanxi, including B (25.0%), C (0.3%), G (0.3%), and CRF01_AE (39.2%), CRF07_BC (32.7%), CRF08_BC (0.8%), CRF55_01B (1.1%), and URFs (0.6%). The subtypes were associated with the transmission routes (χ2 = 77.113, p < 0.01). In this study, a low baseline CD4+ T cell count and a high viral load were found among CRF01_AE-infected patients compared with patients who were infected with non-CRF01_AE (p<0.01) through sexual transmission; however, the CRF01_AE subtype was not associated with a low baseline CD4+ T cell count or a high viral load in Chinese patients infected through blood transmission (p = 0.249). The overall TDR rate in this population was 4.4% between 2003 and 2013. A univariate logistic regression model revealed that a low CD4 T cell count (≤ 100 cells/µL) was associated with the development of drug-resistant strains.
Our work revealed diverse HIV-1 subtype distributions in Shaanxi province. We identified a low and stable TDR time trend among ART-naive patients. These findings enhance our understanding of HIV-1 genetic diversity and provide some guidelines for the improvement and implementation of a comprehensive public health strategy of HIV-1 TDR prevention.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0109821</identifier><identifier>PMID: 25333965</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acquired immune deficiency syndrome ; Adult ; AIDS ; Analysis ; Anti-Retroviral Agents - therapeutic use ; Antiretroviral agents ; Antiretroviral drugs ; Antiretroviral therapy ; Biology and Life Sciences ; Blood ; CD4 antigen ; CD4-Positive T-Lymphocytes - virology ; China ; Disease transmission ; Drug resistance ; Drug Resistance, Viral - genetics ; Drug therapy ; Female ; Genetic diversity ; Genotype ; Genotypes ; Highly active antiretroviral therapy ; HIV ; HIV infections ; HIV Infections - drug therapy ; HIV Infections - genetics ; HIV-1 - genetics ; Human immunodeficiency virus ; Humans ; Lymphocytes T ; Male ; Medicine and health sciences ; Middle Aged ; Occupational health ; Patients ; Phylogeny ; Public health ; Recombination ; Regression analysis ; Regression models ; Ribonucleic acid ; RNA ; RNA, Viral ; Sampling methods ; Sexual transmission ; Sexually transmitted diseases ; STD ; T cells ; Trends ; Young Adult</subject><ispartof>PloS one, 2014-10, Vol.9 (10), p.e109821-e109821</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Zhao et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Zhao et al 2014 Zhao et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-4b30721bff2ba24858616f0d51fe6b24f908ef29c710483e01f70dd4716f08e13</citedby><cites>FETCH-LOGICAL-c692t-4b30721bff2ba24858616f0d51fe6b24f908ef29c710483e01f70dd4716f08e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198111/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198111/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25333965$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Paraskevis, Dimitrios</contributor><creatorcontrib>Zhao, Ke</creatorcontrib><creatorcontrib>Kang, Wenzhen</creatorcontrib><creatorcontrib>Liu, Qingquan</creatorcontrib><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Liu, Qing</creatorcontrib><creatorcontrib>Jiang, Wei</creatorcontrib><creatorcontrib>Zhuang, Yan</creatorcontrib><creatorcontrib>Guo, Zisheng</creatorcontrib><creatorcontrib>Yu, Zhuoran</creatorcontrib><creatorcontrib>Li, Xinhong</creatorcontrib><creatorcontrib>Wang, Chunfu</creatorcontrib><creatorcontrib>Yao, Na</creatorcontrib><creatorcontrib>Sun, Yongtao</creatorcontrib><title>Genotypes and transmitted drug resistance among treatment-naive HIV-1-infected patients in a northwestern province, China: trends from 2003 to 2013</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Transmitted drug resistance (TDR) reduces the efficacy of initial antiretroviral treatment and has become a public health concern. Little information is available regarding the genetic diversity of HIV-1 and the prevalence of TDR among treatment-naïve patients in a northwestern province of China since the implementation of national free antiretroviral therapy (ART).
Blood samples from 372 HIV-1 treatment-naive patients were collected between 2003 and 2013 in Shaanxi province. Viral RNA was extracted for nested PCR, and phylogenetic reconstruction and recombination analyses were performed to characterize patterns of the HIV-1 subtypes. Genotypic drug resistance testing was performed using an in-house assay to determine trends in the prevalence of HIV-1 transmitted drug resistance.
Multiple genotypes were identified among the patients in Shaanxi, including B (25.0%), C (0.3%), G (0.3%), and CRF01_AE (39.2%), CRF07_BC (32.7%), CRF08_BC (0.8%), CRF55_01B (1.1%), and URFs (0.6%). The subtypes were associated with the transmission routes (χ2 = 77.113, p < 0.01). In this study, a low baseline CD4+ T cell count and a high viral load were found among CRF01_AE-infected patients compared with patients who were infected with non-CRF01_AE (p<0.01) through sexual transmission; however, the CRF01_AE subtype was not associated with a low baseline CD4+ T cell count or a high viral load in Chinese patients infected through blood transmission (p = 0.249). The overall TDR rate in this population was 4.4% between 2003 and 2013. A univariate logistic regression model revealed that a low CD4 T cell count (≤ 100 cells/µL) was associated with the development of drug-resistant strains.
Our work revealed diverse HIV-1 subtype distributions in Shaanxi province. We identified a low and stable TDR time trend among ART-naive patients. These findings enhance our understanding of HIV-1 genetic diversity and provide some guidelines for the improvement and implementation of a comprehensive public health strategy of HIV-1 TDR prevention.</description><subject>Acquired immune deficiency syndrome</subject><subject>Adult</subject><subject>AIDS</subject><subject>Analysis</subject><subject>Anti-Retroviral Agents - therapeutic use</subject><subject>Antiretroviral agents</subject><subject>Antiretroviral drugs</subject><subject>Antiretroviral therapy</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>CD4 antigen</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>China</subject><subject>Disease transmission</subject><subject>Drug resistance</subject><subject>Drug Resistance, Viral - genetics</subject><subject>Drug therapy</subject><subject>Female</subject><subject>Genetic diversity</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Highly active antiretroviral therapy</subject><subject>HIV</subject><subject>HIV infections</subject><subject>HIV Infections - drug therapy</subject><subject>HIV Infections - genetics</subject><subject>HIV-1 - genetics</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Medicine and health sciences</subject><subject>Middle Aged</subject><subject>Occupational health</subject><subject>Patients</subject><subject>Phylogeny</subject><subject>Public health</subject><subject>Recombination</subject><subject>Regression analysis</subject><subject>Regression models</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Viral</subject><subject>Sampling methods</subject><subject>Sexual transmission</subject><subject>Sexually transmitted diseases</subject><subject>STD</subject><subject>T cells</subject><subject>Trends</subject><subject>Young 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and transmitted drug resistance among treatment-naive HIV-1-infected patients in a northwestern province, China: trends from 2003 to 2013</title><author>Zhao, Ke ; Kang, Wenzhen ; Liu, Qingquan ; Li, Yuan ; Liu, Qing ; Jiang, Wei ; Zhuang, Yan ; Guo, Zisheng ; Yu, Zhuoran ; Li, Xinhong ; Wang, Chunfu ; Yao, Na ; Sun, Yongtao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-4b30721bff2ba24858616f0d51fe6b24f908ef29c710483e01f70dd4716f08e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acquired immune deficiency syndrome</topic><topic>Adult</topic><topic>AIDS</topic><topic>Analysis</topic><topic>Anti-Retroviral Agents - therapeutic use</topic><topic>Antiretroviral agents</topic><topic>Antiretroviral drugs</topic><topic>Antiretroviral therapy</topic><topic>Biology and Life Sciences</topic><topic>Blood</topic><topic>CD4 antigen</topic><topic>CD4-Positive T-Lymphocytes 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treatment-naive HIV-1-infected patients in a northwestern province, China: trends from 2003 to 2013</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-10-15</date><risdate>2014</risdate><volume>9</volume><issue>10</issue><spage>e109821</spage><epage>e109821</epage><pages>e109821-e109821</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Transmitted drug resistance (TDR) reduces the efficacy of initial antiretroviral treatment and has become a public health concern. Little information is available regarding the genetic diversity of HIV-1 and the prevalence of TDR among treatment-naïve patients in a northwestern province of China since the implementation of national free antiretroviral therapy (ART).
Blood samples from 372 HIV-1 treatment-naive patients were collected between 2003 and 2013 in Shaanxi province. Viral RNA was extracted for nested PCR, and phylogenetic reconstruction and recombination analyses were performed to characterize patterns of the HIV-1 subtypes. Genotypic drug resistance testing was performed using an in-house assay to determine trends in the prevalence of HIV-1 transmitted drug resistance.
Multiple genotypes were identified among the patients in Shaanxi, including B (25.0%), C (0.3%), G (0.3%), and CRF01_AE (39.2%), CRF07_BC (32.7%), CRF08_BC (0.8%), CRF55_01B (1.1%), and URFs (0.6%). The subtypes were associated with the transmission routes (χ2 = 77.113, p < 0.01). In this study, a low baseline CD4+ T cell count and a high viral load were found among CRF01_AE-infected patients compared with patients who were infected with non-CRF01_AE (p<0.01) through sexual transmission; however, the CRF01_AE subtype was not associated with a low baseline CD4+ T cell count or a high viral load in Chinese patients infected through blood transmission (p = 0.249). The overall TDR rate in this population was 4.4% between 2003 and 2013. A univariate logistic regression model revealed that a low CD4 T cell count (≤ 100 cells/µL) was associated with the development of drug-resistant strains.
Our work revealed diverse HIV-1 subtype distributions in Shaanxi province. We identified a low and stable TDR time trend among ART-naive patients. These findings enhance our understanding of HIV-1 genetic diversity and provide some guidelines for the improvement and implementation of a comprehensive public health strategy of HIV-1 TDR prevention.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25333965</pmid><doi>10.1371/journal.pone.0109821</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-10, Vol.9 (10), p.e109821-e109821 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1612298746 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acquired immune deficiency syndrome Adult AIDS Analysis Anti-Retroviral Agents - therapeutic use Antiretroviral agents Antiretroviral drugs Antiretroviral therapy Biology and Life Sciences Blood CD4 antigen CD4-Positive T-Lymphocytes - virology China Disease transmission Drug resistance Drug Resistance, Viral - genetics Drug therapy Female Genetic diversity Genotype Genotypes Highly active antiretroviral therapy HIV HIV infections HIV Infections - drug therapy HIV Infections - genetics HIV-1 - genetics Human immunodeficiency virus Humans Lymphocytes T Male Medicine and health sciences Middle Aged Occupational health Patients Phylogeny Public health Recombination Regression analysis Regression models Ribonucleic acid RNA RNA, Viral Sampling methods Sexual transmission Sexually transmitted diseases STD T cells Trends Young Adult |
| title | Genotypes and transmitted drug resistance among treatment-naive HIV-1-infected patients in a northwestern province, China: trends from 2003 to 2013 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T14%3A16%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genotypes%20and%20transmitted%20drug%20resistance%20among%20treatment-naive%20HIV-1-infected%20patients%20in%20a%20northwestern%20province,%20China:%20trends%20from%202003%20to%202013&rft.jtitle=PloS%20one&rft.au=Zhao,%20Ke&rft.date=2014-10-15&rft.volume=9&rft.issue=10&rft.spage=e109821&rft.epage=e109821&rft.pages=e109821-e109821&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0109821&rft_dat=%3Cgale_plos_%3EA418530142%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1612298746&rft_id=info:pmid/25333965&rft_galeid=A418530142&rft_doaj_id=oai_doaj_org_article_9ea8f73f83224e6a8d9c4ace9d669ad9&rfr_iscdi=true |