Guidance of mesenchymal stem cells on fibronectin structured hydrogel films
Designing of implant surfaces using a suitable ligand for cell adhesion to stimulate specific biological responses of stem cells will boost the application of regenerative implants. For example, materials that facilitate rapid and guided migration of stem cells would promote tissue regeneration. Whe...
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| Veröffentlicht in: | PloS one 2014-10, Vol.9 (10), p.e109411-e109411 |
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| creator | Kasten, Annika Naser, Tamara Brüllhoff, Kristina Fiedler, Jörg Müller, Petra Möller, Martin Rychly, Joachim Groll, Jürgen Brenner, Rolf E |
| description | Designing of implant surfaces using a suitable ligand for cell adhesion to stimulate specific biological responses of stem cells will boost the application of regenerative implants. For example, materials that facilitate rapid and guided migration of stem cells would promote tissue regeneration. When seeded on fibronectin (FN) that was homogeneously immmobilized to NCO-sP(EO-stat-PO), which otherwise prevents protein binding and cell adhesion, human mesenchymal stem cells (MSC) revealed a faster migration, increased spreading and a more rapid organization of different cellular components for cell adhesion on fibronectin than on a glass surface. To further explore, how a structural organization of FN controls the behavior of MSC, adhesive lines of FN with varying width between 10 µm and 80 µm and spacings between 5 µm and 20 µm that did not allow cell adhesion were generated. In dependance on both line width and gaps, cells formed adjacent cell contacts, were individually organized in lines, or bridged the lines. With decreasing sizes of FN lines, speed and directionality of cell migration increased, which correlated with organization of the actin cytoskeleton, size and shape of the nuclei as well as of focal adhesions. Together, defined FN lines and gaps enabled a fine tuning of the structural organization of cellular components and migration. Microstructured adhesive substrates can mimic the extracellular matrix in vivo and stimulate cellular mechanisms which play a role in tissue regeneration. |
| doi_str_mv | 10.1371/journal.pone.0109411 |
| format | Article |
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For example, materials that facilitate rapid and guided migration of stem cells would promote tissue regeneration. When seeded on fibronectin (FN) that was homogeneously immmobilized to NCO-sP(EO-stat-PO), which otherwise prevents protein binding and cell adhesion, human mesenchymal stem cells (MSC) revealed a faster migration, increased spreading and a more rapid organization of different cellular components for cell adhesion on fibronectin than on a glass surface. To further explore, how a structural organization of FN controls the behavior of MSC, adhesive lines of FN with varying width between 10 µm and 80 µm and spacings between 5 µm and 20 µm that did not allow cell adhesion were generated. In dependance on both line width and gaps, cells formed adjacent cell contacts, were individually organized in lines, or bridged the lines. With decreasing sizes of FN lines, speed and directionality of cell migration increased, which correlated with organization of the actin cytoskeleton, size and shape of the nuclei as well as of focal adhesions. Together, defined FN lines and gaps enabled a fine tuning of the structural organization of cellular components and migration. 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Microstructured adhesive substrates can mimic the extracellular matrix in vivo and stimulate cellular mechanisms which play a role in tissue regeneration.</description><subject>Actin</subject><subject>Actins - metabolism</subject><subject>Adhesion</subject><subject>Adhesives</subject><subject>Biochemistry</subject><subject>Biology</subject><subject>Biology and Life Sciences</subject><subject>Cell adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Line</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Cellular structure</subject><subject>Cytoskeleton</subject><subject>Cytoskeleton - drug effects</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - drug effects</subject><subject>Extracellular Matrix - physiology</subject><subject>Fibronectin</subject><subject>Fibronectins</subject><subject>Fibronectins - pharmacology</subject><subject>Gene expression</subject><subject>Glass substrates</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Laboratories</subject><subject>Ligands</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kasten, Annika</au><au>Naser, Tamara</au><au>Brüllhoff, Kristina</au><au>Fiedler, Jörg</au><au>Müller, Petra</au><au>Möller, Martin</au><au>Rychly, Joachim</au><au>Groll, Jürgen</au><au>Brenner, Rolf E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Guidance of mesenchymal stem cells on fibronectin structured hydrogel films</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-10-15</date><risdate>2014</risdate><volume>9</volume><issue>10</issue><spage>e109411</spage><epage>e109411</epage><pages>e109411-e109411</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Designing of implant surfaces using a suitable ligand for cell adhesion to stimulate specific biological responses of stem cells will boost the application of regenerative implants. For example, materials that facilitate rapid and guided migration of stem cells would promote tissue regeneration. When seeded on fibronectin (FN) that was homogeneously immmobilized to NCO-sP(EO-stat-PO), which otherwise prevents protein binding and cell adhesion, human mesenchymal stem cells (MSC) revealed a faster migration, increased spreading and a more rapid organization of different cellular components for cell adhesion on fibronectin than on a glass surface. To further explore, how a structural organization of FN controls the behavior of MSC, adhesive lines of FN with varying width between 10 µm and 80 µm and spacings between 5 µm and 20 µm that did not allow cell adhesion were generated. In dependance on both line width and gaps, cells formed adjacent cell contacts, were individually organized in lines, or bridged the lines. With decreasing sizes of FN lines, speed and directionality of cell migration increased, which correlated with organization of the actin cytoskeleton, size and shape of the nuclei as well as of focal adhesions. Together, defined FN lines and gaps enabled a fine tuning of the structural organization of cellular components and migration. Microstructured adhesive substrates can mimic the extracellular matrix in vivo and stimulate cellular mechanisms which play a role in tissue regeneration.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25329487</pmid><doi>10.1371/journal.pone.0109411</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Actin Actins - metabolism Adhesion Adhesives Biochemistry Biology Biology and Life Sciences Cell adhesion Cell adhesion & migration Cell Adhesion - drug effects Cell Line Cell migration Cell Movement - drug effects Cellular structure Cytoskeleton Cytoskeleton - drug effects Extracellular matrix Extracellular Matrix - drug effects Extracellular Matrix - physiology Fibronectin Fibronectins Fibronectins - pharmacology Gene expression Glass substrates Humans Hydrogels Laboratories Ligands Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Mesenchyme Military leaders Muscle proteins Nuclei Protein binding Proteins Regeneration Stem cells Substrates Surgery Surgical implants Tissue engineering |
| title | Guidance of mesenchymal stem cells on fibronectin structured hydrogel films |
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