Verifying the stability of selected genes for normalization in Q PCR experiments of Spodoptera frugiperda cells during AcMNPV infection

It is challenging to find genes with stable transcripts for use as reference genes for quantitative realtime polymerase chain reaction (qRT-PCR) during viral infection. Autographa californica nucleopolyhedrovirus (AcMNPV) is known to globally shut off host gene transcription in Sf21 cells and to mod...

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Veröffentlicht in:	PloS one 2014-10, Vol.9 (10), p.e108516-e108516
Hauptverfasser:	Salem, Tamer Z, Allam, Walaa R, Thiem, Suzanne M
Format:	Artikel
Sprache:	eng
Schlagworte:	Actin Actins - genetics Actins - metabolism Animals Autographa californica Biology and Life Sciences Cell cycle Cytoskeleton Data analysis Data processing DNA microarrays Gene expression Genes Genetic engineering Genetic Vectors - metabolism Glyceraldehyde Glyceraldehyde 3-phosphate Glyceraldehyde 3-Phosphate - genetics Glyceraldehyde 3-Phosphate - metabolism Glyceraldehyde-3-phosphate dehydrogenase Infections Myosin Myosins - genetics Myosins - metabolism Nucleopolyhedroviruses - genetics Polymerase chain reaction Real-Time Polymerase Chain Reaction Ribonucleic acid Ribosomal protein L35 RNA RNA, Ribosomal, 28S - metabolism rRNA 18S rRNA 28S Science Sf9 Cells Spodoptera Spodoptera frugiperda Transcription Transcriptome Transfection Tubulin Tubulin - genetics Tubulin - metabolism Viral infections Viruses
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