RASSF1A promoter hypermethylation is a strong biomarker of poor survival in patients with salivary adenoid cystic carcinoma in a Chinese population

In addition to the clinicopathological parameters, molecular biomarkers are becoming increasingly important in the prognostic evaluation of cancer patients. This study aimed to determine the molecular alterations in the RAS association domain family protein1A gene (RASSF1A) in salivary adenoid cysti...

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Veröffentlicht in:PloS one 2014-10, Vol.9 (10), p.e110159-e110159
Hauptverfasser: Zhang, Chun-Ye, Zhao, Yang-Xing, Xia, Rong-Hui, Han, Jing, Wang, Bing-Shun, Tian, Zhen, Wang, Li-Zhen, Hu, Yu-Hua, Li, Jiang
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container_title PloS one
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creator Zhang, Chun-Ye
Zhao, Yang-Xing
Xia, Rong-Hui
Han, Jing
Wang, Bing-Shun
Tian, Zhen
Wang, Li-Zhen
Hu, Yu-Hua
Li, Jiang
description In addition to the clinicopathological parameters, molecular biomarkers are becoming increasingly important in the prognostic evaluation of cancer patients. This study aimed to determine the molecular alterations in the RAS association domain family protein1A gene (RASSF1A) in salivary adenoid cystic carcinoma (ACC) and to evaluate the potential of such alterations as prognostic markers. One hundred and sixty-seven ACC tumor tissues and 50 samples of matched normal salivary gland tissues from the same patients were analyzed for RASSF1A promoter methylation status by bisulfite sequencing PCR (BSP) and/or methylation-specific PCR (MSP). Fifty ACC tumor tissues and matched normal salivary gland tissues were analyzed for loss of heterozygosity (LOH) by examining two microsatellite markers (D3S1478, D3S1621) at 3p21. RASSF1A gene mutations were detected by direct sequencing of all six exons in 50 tumor and normal tissue specimens. Over-all, RASSF1A promoter hypermethylation was detected in 35.3% (59/167) of ACC tissues and was associated with histologically solid tumor pattern (P = 0.002) and advanced TNM stage (P = 0.014). RASSF1A LOH was observed in 18.0% (9/50) of cases, and no somatic mutation of RASSF1A was detected in any cases. RASSF1A promoter methylation was associated with the poor over-all survival (Log-rank test, P
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This study aimed to determine the molecular alterations in the RAS association domain family protein1A gene (RASSF1A) in salivary adenoid cystic carcinoma (ACC) and to evaluate the potential of such alterations as prognostic markers. One hundred and sixty-seven ACC tumor tissues and 50 samples of matched normal salivary gland tissues from the same patients were analyzed for RASSF1A promoter methylation status by bisulfite sequencing PCR (BSP) and/or methylation-specific PCR (MSP). Fifty ACC tumor tissues and matched normal salivary gland tissues were analyzed for loss of heterozygosity (LOH) by examining two microsatellite markers (D3S1478, D3S1621) at 3p21. RASSF1A gene mutations were detected by direct sequencing of all six exons in 50 tumor and normal tissue specimens. Over-all, RASSF1A promoter hypermethylation was detected in 35.3% (59/167) of ACC tissues and was associated with histologically solid tumor pattern (P = 0.002) and advanced TNM stage (P = 0.014). RASSF1A LOH was observed in 18.0% (9/50) of cases, and no somatic mutation of RASSF1A was detected in any cases. RASSF1A promoter methylation was associated with the poor over-all survival (Log-rank test, P &lt;0.001) and disease-free survival (Log-rank test, P &lt;0.001) and identified as an independent predicator of over-all patient survival (P = 0.009) and disease-free survival (P &lt;0.001). It was concluded that RASSF1A methylation is involved in the development, differentiation and progression of ACC and is a strong independent biomarker of poor survival in ACC patients in a Chinese population.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0110159</identifier><identifier>PMID: 25302792</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenoid ; Adult ; Aged ; Aged, 80 and over ; Analysis ; Asian Continental Ancestry Group - genetics ; Bioindicators ; Biomarkers ; Biomarkers, Tumor ; Bisulfite ; Breast cancer ; Carcinoma ; Carcinoma, Adenoid Cystic - genetics ; Carcinoma, Adenoid Cystic - mortality ; Carcinoma, Adenoid Cystic - pathology ; Care and treatment ; China ; Chromosome 3 ; DNA Methylation ; DNA Mutational Analysis ; Exons ; Female ; Gene sequencing ; Genetic markers ; Heterozygosity ; Humans ; Kaplan-Meier Estimate ; Kinases ; Loss of Heterozygosity ; Male ; Medicine and Health Sciences ; Methylation ; Microsatellites ; Middle Aged ; Mutation ; Neoplasm Staging ; Oral cancer ; Patient outcomes ; Patients ; Prognosis ; Promoter Regions, Genetic ; Proportional Hazards Models ; Salivary gland ; Salivary Gland Neoplasms - genetics ; Salivary Gland Neoplasms - mortality ; Salivary Gland Neoplasms - pathology ; Solid tumors ; Survival ; Tissues ; Tumor Suppressor Proteins - genetics</subject><ispartof>PloS one, 2014-10, Vol.9 (10), p.e110159-e110159</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Zhang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Zhang et al 2014 Zhang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-a9d54c077426d36b4af62faa74cd65551f96a6770351fb6c5616528c4a1ec86c3</citedby><cites>FETCH-LOGICAL-c692t-a9d54c077426d36b4af62faa74cd65551f96a6770351fb6c5616528c4a1ec86c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193867/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193867/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25302792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lo, Anthony W.I.</contributor><creatorcontrib>Zhang, Chun-Ye</creatorcontrib><creatorcontrib>Zhao, Yang-Xing</creatorcontrib><creatorcontrib>Xia, Rong-Hui</creatorcontrib><creatorcontrib>Han, Jing</creatorcontrib><creatorcontrib>Wang, Bing-Shun</creatorcontrib><creatorcontrib>Tian, Zhen</creatorcontrib><creatorcontrib>Wang, Li-Zhen</creatorcontrib><creatorcontrib>Hu, Yu-Hua</creatorcontrib><creatorcontrib>Li, Jiang</creatorcontrib><title>RASSF1A promoter hypermethylation is a strong biomarker of poor survival in patients with salivary adenoid cystic carcinoma in a Chinese population</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In addition to the clinicopathological parameters, molecular biomarkers are becoming increasingly important in the prognostic evaluation of cancer patients. This study aimed to determine the molecular alterations in the RAS association domain family protein1A gene (RASSF1A) in salivary adenoid cystic carcinoma (ACC) and to evaluate the potential of such alterations as prognostic markers. One hundred and sixty-seven ACC tumor tissues and 50 samples of matched normal salivary gland tissues from the same patients were analyzed for RASSF1A promoter methylation status by bisulfite sequencing PCR (BSP) and/or methylation-specific PCR (MSP). Fifty ACC tumor tissues and matched normal salivary gland tissues were analyzed for loss of heterozygosity (LOH) by examining two microsatellite markers (D3S1478, D3S1621) at 3p21. RASSF1A gene mutations were detected by direct sequencing of all six exons in 50 tumor and normal tissue specimens. Over-all, RASSF1A promoter hypermethylation was detected in 35.3% (59/167) of ACC tissues and was associated with histologically solid tumor pattern (P = 0.002) and advanced TNM stage (P = 0.014). RASSF1A LOH was observed in 18.0% (9/50) of cases, and no somatic mutation of RASSF1A was detected in any cases. RASSF1A promoter methylation was associated with the poor over-all survival (Log-rank test, P &lt;0.001) and disease-free survival (Log-rank test, P &lt;0.001) and identified as an independent predicator of over-all patient survival (P = 0.009) and disease-free survival (P &lt;0.001). It was concluded that RASSF1A methylation is involved in the development, differentiation and progression of ACC and is a strong independent biomarker of poor survival in ACC patients in a Chinese population.</description><subject>Adenoid</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Analysis</subject><subject>Asian Continental Ancestry Group - genetics</subject><subject>Bioindicators</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor</subject><subject>Bisulfite</subject><subject>Breast cancer</subject><subject>Carcinoma</subject><subject>Carcinoma, Adenoid Cystic - genetics</subject><subject>Carcinoma, Adenoid Cystic - mortality</subject><subject>Carcinoma, Adenoid Cystic - pathology</subject><subject>Care and treatment</subject><subject>China</subject><subject>Chromosome 3</subject><subject>DNA Methylation</subject><subject>DNA Mutational Analysis</subject><subject>Exons</subject><subject>Female</subject><subject>Gene sequencing</subject><subject>Genetic markers</subject><subject>Heterozygosity</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Kinases</subject><subject>Loss of Heterozygosity</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Methylation</subject><subject>Microsatellites</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Neoplasm Staging</subject><subject>Oral cancer</subject><subject>Patient outcomes</subject><subject>Patients</subject><subject>Prognosis</subject><subject>Promoter Regions, Genetic</subject><subject>Proportional Hazards Models</subject><subject>Salivary gland</subject><subject>Salivary Gland Neoplasms - genetics</subject><subject>Salivary Gland Neoplasms - mortality</subject><subject>Salivary Gland Neoplasms - pathology</subject><subject>Solid tumors</subject><subject>Survival</subject><subject>Tissues</subject><subject>Tumor Suppressor Proteins - genetics</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99u0zAUxiMEYmPwBggsISG4aPGfxElukKqKQaVJk1bg1jp1nMbFjYPtFPocvDDumk0N2gXyhS37933H59gnSV4SPCUsJx82tnctmGlnWzXFhGCSlY-Sc1IyOuEUs8cn67PkmfcbjDNWcP40OaMZwzQv6Xny52a2XF6SGeqc3dqgHGr2nXJbFZq9gaBti7RHgHxwtl2jlbZbcD8iZmvUWeuQ791O78Ag3aIuClQbPPqlQ4M8mHjg9ggq1VpdIbn3QUskwUndRp-DBNC80a3yKrp1_THi8-RJDcarF8N8kXy7_PR1_mVydf15MZ9dTSQvaZhAWWWpxHmeUl4xvkqh5rQGyFNZ8SzLSF1y4HmOWVyuuMw44RktZApEyYJLdpG8Pvp2xnox1NMLwmMtGcvSMhKLI1FZ2IjO6Zj8XljQ4nbDurUAF3MySsi8rnGpCpnjImUVlBRSpSper1RGecGi18chWr_aqkrGQjkwI9PxSasbsbY7kcZnLHgeDd4NBs7-7JUPYqu9VMZAq2x_e29Ki4JhHNE3_6APZzdQa4gJ6La2Ma48mIpZSgrOshynkZo-QMVRqa2W8fPVOu6PBO9HgsgE9TusofdeLJY3_89efx-zb0_YRoEJjbemP3wZPwbTIyid9d6p-r7IBItD79xVQxx6Rwy9E2WvTh_oXnTXLOwvjGkWRw</recordid><startdate>20141010</startdate><enddate>20141010</enddate><creator>Zhang, Chun-Ye</creator><creator>Zhao, Yang-Xing</creator><creator>Xia, Rong-Hui</creator><creator>Han, Jing</creator><creator>Wang, Bing-Shun</creator><creator>Tian, Zhen</creator><creator>Wang, Li-Zhen</creator><creator>Hu, Yu-Hua</creator><creator>Li, Jiang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20141010</creationdate><title>RASSF1A promoter hypermethylation is a strong biomarker of poor survival in patients with salivary adenoid cystic carcinoma in a Chinese population</title><author>Zhang, Chun-Ye ; Zhao, Yang-Xing ; Xia, Rong-Hui ; Han, Jing ; Wang, Bing-Shun ; Tian, Zhen ; Wang, Li-Zhen ; Hu, Yu-Hua ; Li, Jiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-a9d54c077426d36b4af62faa74cd65551f96a6770351fb6c5616528c4a1ec86c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenoid</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Analysis</topic><topic>Asian Continental Ancestry Group - genetics</topic><topic>Bioindicators</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor</topic><topic>Bisulfite</topic><topic>Breast cancer</topic><topic>Carcinoma</topic><topic>Carcinoma, Adenoid Cystic - genetics</topic><topic>Carcinoma, Adenoid Cystic - mortality</topic><topic>Carcinoma, Adenoid Cystic - pathology</topic><topic>Care and treatment</topic><topic>China</topic><topic>Chromosome 3</topic><topic>DNA Methylation</topic><topic>DNA Mutational Analysis</topic><topic>Exons</topic><topic>Female</topic><topic>Gene sequencing</topic><topic>Genetic markers</topic><topic>Heterozygosity</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Kinases</topic><topic>Loss of Heterozygosity</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Methylation</topic><topic>Microsatellites</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>Neoplasm Staging</topic><topic>Oral cancer</topic><topic>Patient outcomes</topic><topic>Patients</topic><topic>Prognosis</topic><topic>Promoter Regions, Genetic</topic><topic>Proportional Hazards Models</topic><topic>Salivary gland</topic><topic>Salivary Gland Neoplasms - genetics</topic><topic>Salivary Gland Neoplasms - mortality</topic><topic>Salivary Gland Neoplasms - pathology</topic><topic>Solid tumors</topic><topic>Survival</topic><topic>Tissues</topic><topic>Tumor Suppressor Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Chun-Ye</creatorcontrib><creatorcontrib>Zhao, Yang-Xing</creatorcontrib><creatorcontrib>Xia, Rong-Hui</creatorcontrib><creatorcontrib>Han, Jing</creatorcontrib><creatorcontrib>Wang, Bing-Shun</creatorcontrib><creatorcontrib>Tian, Zhen</creatorcontrib><creatorcontrib>Wang, Li-Zhen</creatorcontrib><creatorcontrib>Hu, Yu-Hua</creatorcontrib><creatorcontrib>Li, Jiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Chun-Ye</au><au>Zhao, Yang-Xing</au><au>Xia, Rong-Hui</au><au>Han, Jing</au><au>Wang, Bing-Shun</au><au>Tian, Zhen</au><au>Wang, Li-Zhen</au><au>Hu, Yu-Hua</au><au>Li, Jiang</au><au>Lo, Anthony W.I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RASSF1A promoter hypermethylation is a strong biomarker of poor survival in patients with salivary adenoid cystic carcinoma in a Chinese population</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-10-10</date><risdate>2014</risdate><volume>9</volume><issue>10</issue><spage>e110159</spage><epage>e110159</epage><pages>e110159-e110159</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>In addition to the clinicopathological parameters, molecular biomarkers are becoming increasingly important in the prognostic evaluation of cancer patients. This study aimed to determine the molecular alterations in the RAS association domain family protein1A gene (RASSF1A) in salivary adenoid cystic carcinoma (ACC) and to evaluate the potential of such alterations as prognostic markers. One hundred and sixty-seven ACC tumor tissues and 50 samples of matched normal salivary gland tissues from the same patients were analyzed for RASSF1A promoter methylation status by bisulfite sequencing PCR (BSP) and/or methylation-specific PCR (MSP). Fifty ACC tumor tissues and matched normal salivary gland tissues were analyzed for loss of heterozygosity (LOH) by examining two microsatellite markers (D3S1478, D3S1621) at 3p21. RASSF1A gene mutations were detected by direct sequencing of all six exons in 50 tumor and normal tissue specimens. Over-all, RASSF1A promoter hypermethylation was detected in 35.3% (59/167) of ACC tissues and was associated with histologically solid tumor pattern (P = 0.002) and advanced TNM stage (P = 0.014). RASSF1A LOH was observed in 18.0% (9/50) of cases, and no somatic mutation of RASSF1A was detected in any cases. RASSF1A promoter methylation was associated with the poor over-all survival (Log-rank test, P &lt;0.001) and disease-free survival (Log-rank test, P &lt;0.001) and identified as an independent predicator of over-all patient survival (P = 0.009) and disease-free survival (P &lt;0.001). It was concluded that RASSF1A methylation is involved in the development, differentiation and progression of ACC and is a strong independent biomarker of poor survival in ACC patients in a Chinese population.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25302792</pmid><doi>10.1371/journal.pone.0110159</doi><oa>free_for_read</oa></addata></record>
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ispartof PloS one, 2014-10, Vol.9 (10), p.e110159-e110159
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1932-6203
language eng
recordid cdi_plos_journals_1610133549
source MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry
subjects Adenoid
Adult
Aged
Aged, 80 and over
Analysis
Asian Continental Ancestry Group - genetics
Bioindicators
Biomarkers
Biomarkers, Tumor
Bisulfite
Breast cancer
Carcinoma
Carcinoma, Adenoid Cystic - genetics
Carcinoma, Adenoid Cystic - mortality
Carcinoma, Adenoid Cystic - pathology
Care and treatment
China
Chromosome 3
DNA Methylation
DNA Mutational Analysis
Exons
Female
Gene sequencing
Genetic markers
Heterozygosity
Humans
Kaplan-Meier Estimate
Kinases
Loss of Heterozygosity
Male
Medicine and Health Sciences
Methylation
Microsatellites
Middle Aged
Mutation
Neoplasm Staging
Oral cancer
Patient outcomes
Patients
Prognosis
Promoter Regions, Genetic
Proportional Hazards Models
Salivary gland
Salivary Gland Neoplasms - genetics
Salivary Gland Neoplasms - mortality
Salivary Gland Neoplasms - pathology
Solid tumors
Survival
Tissues
Tumor Suppressor Proteins - genetics
title RASSF1A promoter hypermethylation is a strong biomarker of poor survival in patients with salivary adenoid cystic carcinoma in a Chinese population
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