Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability
Vascular calcification, recapitulating bone formation, has a profound impact on plaque stability. The aim of the present study was to determine the influence of bone-like vascular calcification (named osteoid metaplasia = OM) and of osteoprotegerin on plaque stability. Tissue from carotid endarterec...
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| creator | Davaine, Jean-Michel Quillard, Thibaut Brion, Régis Lapérine, Olivier Guyomarch, Béatrice Merlini, Thierry Chatelais, Mathias Guilbaud, Florian Brennan, Meadhbh Áine Charrier, Céline Heymann, Dominique Gouëffic, Yann Heymann, Marie-Françoise |
| description | Vascular calcification, recapitulating bone formation, has a profound impact on plaque stability. The aim of the present study was to determine the influence of bone-like vascular calcification (named osteoid metaplasia = OM) and of osteoprotegerin on plaque stability.
Tissue from carotid endarterectomies were analysed for the presence of calcification and signs of vulnerability according to AHA grading system. Osteoprotegerin (OPG), pericytes and endothelial cells were sought using immuno-histochemistry. Symptoms and preoperative imaging findings (CT-scan, MRI and Doppler-scan) were analyzed. Human pericytes were cultured to evaluate their ability to secrete OPG and to influence mineralization in the plaque.
Seventy-three carotid plaques (49 asymptomatic and 24 symptomatic) were harvested. A significantly higher presence of OM (18.4% vs 0%, p |
| doi_str_mv | 10.1371/journal.pone.0107642 |
| format | Article |
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Tissue from carotid endarterectomies were analysed for the presence of calcification and signs of vulnerability according to AHA grading system. Osteoprotegerin (OPG), pericytes and endothelial cells were sought using immuno-histochemistry. Symptoms and preoperative imaging findings (CT-scan, MRI and Doppler-scan) were analyzed. Human pericytes were cultured to evaluate their ability to secrete OPG and to influence mineralization in the plaque.
Seventy-three carotid plaques (49 asymptomatic and 24 symptomatic) were harvested. A significantly higher presence of OM (18.4% vs 0%, p<0.01), OPG (10.2% of ROI vs 3.4% of ROI, p<0.05) and pericytes (19% of ROI vs 3.8% of ROI, p<0.05) were noted in asymptomatic compared to symptomatic plaques. Consistently, circulating OPG levels were higher in the plasma of asymptomatic patients (3.2 ng/mL vs 2.5 ng/mL, p = 0.05). In vitro, human vascular pericytes secreted considerable amounts of OPG and underwent osteoblastic differentiation. Pericytes also inhibited the osteoclastic differentiation of CD14+ cells through their secretion of OPG.
OPG (intraplaque an plasmatic) and OM are associated with carotid plaque stability. Pericytes may be involved in the secretion of intraplaque OPG and in the formation of OM.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0107642</identifier><identifier>PMID: 25259713</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Atherosclerosis ; Biocompatibility ; Biology and Life Sciences ; Biomedical materials ; Bone growth ; Bones ; Calcification ; Calcification (ectopic) ; Cardiovascular disease ; Carotid Stenosis - diagnosis ; Carotid Stenosis - metabolism ; Carotid Stenosis - pathology ; CD14 antigen ; CD146 Antigen - metabolism ; Cell Differentiation ; Computed tomography ; Cytokines ; Differentiation ; Endothelial cells ; Evaluation ; Family medical history ; Histochemistry ; Homeostasis ; Humans ; Ligands ; Magnetic resonance imaging ; Medicine and Health Sciences ; Metaplasia ; Mineralization ; Mortality ; Osteoblastogenesis ; Osteoblasts ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Osteoclastogenesis ; Osteoclasts ; Osteogenesis ; Osteoid ; Osteoprotegerin ; Osteoprotegerin - blood ; Osteoprotegerin - metabolism ; Patients ; Pericytes ; Pericytes - cytology ; Pericytes - metabolism ; Plaque, Atherosclerotic - metabolism ; Plaque, Atherosclerotic - pathology ; Plaques ; RANK Ligand - metabolism ; Rodents ; Stability analysis ; Stroke ; Studies ; Surgery ; Tumor necrosis factor-TNF ; Vascular Calcification</subject><ispartof>PloS one, 2014-09, Vol.9 (9), p.e107642-e107642</ispartof><rights>2014 Davaine et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Davaine et al 2014 Davaine et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-1e9e412e34d0b7839192795d52fcef4b89828812ac2329d7ec4cf0df082e92ca3</citedby><cites>FETCH-LOGICAL-c596t-1e9e412e34d0b7839192795d52fcef4b89828812ac2329d7ec4cf0df082e92ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178031/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178031/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25259713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Davaine, Jean-Michel</creatorcontrib><creatorcontrib>Quillard, Thibaut</creatorcontrib><creatorcontrib>Brion, Régis</creatorcontrib><creatorcontrib>Lapérine, Olivier</creatorcontrib><creatorcontrib>Guyomarch, Béatrice</creatorcontrib><creatorcontrib>Merlini, Thierry</creatorcontrib><creatorcontrib>Chatelais, Mathias</creatorcontrib><creatorcontrib>Guilbaud, Florian</creatorcontrib><creatorcontrib>Brennan, Meadhbh Áine</creatorcontrib><creatorcontrib>Charrier, Céline</creatorcontrib><creatorcontrib>Heymann, Dominique</creatorcontrib><creatorcontrib>Gouëffic, Yann</creatorcontrib><creatorcontrib>Heymann, Marie-Françoise</creatorcontrib><title>Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Vascular calcification, recapitulating bone formation, has a profound impact on plaque stability. The aim of the present study was to determine the influence of bone-like vascular calcification (named osteoid metaplasia = OM) and of osteoprotegerin on plaque stability.
Tissue from carotid endarterectomies were analysed for the presence of calcification and signs of vulnerability according to AHA grading system. Osteoprotegerin (OPG), pericytes and endothelial cells were sought using immuno-histochemistry. Symptoms and preoperative imaging findings (CT-scan, MRI and Doppler-scan) were analyzed. Human pericytes were cultured to evaluate their ability to secrete OPG and to influence mineralization in the plaque.
Seventy-three carotid plaques (49 asymptomatic and 24 symptomatic) were harvested. A significantly higher presence of OM (18.4% vs 0%, p<0.01), OPG (10.2% of ROI vs 3.4% of ROI, p<0.05) and pericytes (19% of ROI vs 3.8% of ROI, p<0.05) were noted in asymptomatic compared to symptomatic plaques. Consistently, circulating OPG levels were higher in the plasma of asymptomatic patients (3.2 ng/mL vs 2.5 ng/mL, p = 0.05). In vitro, human vascular pericytes secreted considerable amounts of OPG and underwent osteoblastic differentiation. Pericytes also inhibited the osteoclastic differentiation of CD14+ cells through their secretion of OPG.
OPG (intraplaque an plasmatic) and OM are associated with carotid plaque stability. Pericytes may be involved in the secretion of intraplaque OPG and in the formation of OM.</description><subject>Atherosclerosis</subject><subject>Biocompatibility</subject><subject>Biology and Life Sciences</subject><subject>Biomedical materials</subject><subject>Bone growth</subject><subject>Bones</subject><subject>Calcification</subject><subject>Calcification (ectopic)</subject><subject>Cardiovascular disease</subject><subject>Carotid Stenosis - diagnosis</subject><subject>Carotid Stenosis - metabolism</subject><subject>Carotid Stenosis - pathology</subject><subject>CD14 antigen</subject><subject>CD146 Antigen - metabolism</subject><subject>Cell Differentiation</subject><subject>Computed tomography</subject><subject>Cytokines</subject><subject>Differentiation</subject><subject>Endothelial cells</subject><subject>Evaluation</subject><subject>Family medical history</subject><subject>Histochemistry</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Ligands</subject><subject>Magnetic resonance imaging</subject><subject>Medicine and Health Sciences</subject><subject>Metaplasia</subject><subject>Mineralization</subject><subject>Mortality</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteoclastogenesis</subject><subject>Osteoclasts</subject><subject>Osteogenesis</subject><subject>Osteoid</subject><subject>Osteoprotegerin</subject><subject>Osteoprotegerin - blood</subject><subject>Osteoprotegerin - metabolism</subject><subject>Patients</subject><subject>Pericytes</subject><subject>Pericytes - cytology</subject><subject>Pericytes - metabolism</subject><subject>Plaque, Atherosclerotic - metabolism</subject><subject>Plaque, Atherosclerotic - pathology</subject><subject>Plaques</subject><subject>RANK Ligand - metabolism</subject><subject>Rodents</subject><subject>Stability analysis</subject><subject>Stroke</subject><subject>Studies</subject><subject>Surgery</subject><subject>Tumor necrosis factor-TNF</subject><subject>Vascular Calcification</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptkktv1DAUhSMEoqXwDxBEYsOiGfyKY2-QUAW0UqVuYG3dODdTD5442J6i-fe4nbRqEStb9jnffehU1VtKVpR39NMm7OIEfjWHCVeEkk4K9qw6ppqzRjLCnz-6H1WvUtoQ0nIl5cvqiLWs1R3lx5W_ShnDHEPGNUY3ndZzOew-Y6phGuq-0BvvfmF9A8nuPMTagrdudBayC1MNEWtIKVgHGYf6j8vXRVF4bqhnD793WKcMvfMu719XL0bwCd8s50n189vXH2fnzeXV94uzL5eNbbXMDUWNgjLkYiB9p7immnW6HVo2WhxFr7RiSlEGlnGmhw6tsCMZRqIYamaBn1TvD9zZh2SWRSVDW9lKTYqpKC4OiiHAxszRbSHuTQBn7h5CXBuI2VmPRvQCyKjF0GklWgSFXI69ZHxklBOpC-vzUm3Xb3GwOOUI_gn06c_krs063BhBO0U4LYCPCyCGsq-UzdYli97DhGF317dUpZgURfrhH-n_pxMHlY0hpYjjQzOUmNvw3LvMbXjMEp5ie_d4kAfTfVr4X-fcxGI</recordid><startdate>20140926</startdate><enddate>20140926</enddate><creator>Davaine, Jean-Michel</creator><creator>Quillard, Thibaut</creator><creator>Brion, Régis</creator><creator>Lapérine, Olivier</creator><creator>Guyomarch, Béatrice</creator><creator>Merlini, Thierry</creator><creator>Chatelais, Mathias</creator><creator>Guilbaud, Florian</creator><creator>Brennan, Meadhbh Áine</creator><creator>Charrier, Céline</creator><creator>Heymann, Dominique</creator><creator>Gouëffic, Yann</creator><creator>Heymann, Marie-Françoise</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140926</creationdate><title>Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability</title><author>Davaine, Jean-Michel ; Quillard, Thibaut ; Brion, Régis ; Lapérine, Olivier ; Guyomarch, Béatrice ; Merlini, Thierry ; Chatelais, Mathias ; Guilbaud, Florian ; Brennan, Meadhbh Áine ; Charrier, Céline ; Heymann, Dominique ; Gouëffic, Yann ; Heymann, Marie-Françoise</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-1e9e412e34d0b7839192795d52fcef4b89828812ac2329d7ec4cf0df082e92ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Atherosclerosis</topic><topic>Biocompatibility</topic><topic>Biology and Life Sciences</topic><topic>Biomedical materials</topic><topic>Bone growth</topic><topic>Bones</topic><topic>Calcification</topic><topic>Calcification (ectopic)</topic><topic>Cardiovascular disease</topic><topic>Carotid Stenosis - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davaine, Jean-Michel</au><au>Quillard, Thibaut</au><au>Brion, Régis</au><au>Lapérine, Olivier</au><au>Guyomarch, Béatrice</au><au>Merlini, Thierry</au><au>Chatelais, Mathias</au><au>Guilbaud, Florian</au><au>Brennan, Meadhbh Áine</au><au>Charrier, Céline</au><au>Heymann, Dominique</au><au>Gouëffic, Yann</au><au>Heymann, Marie-Françoise</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-09-26</date><risdate>2014</risdate><volume>9</volume><issue>9</issue><spage>e107642</spage><epage>e107642</epage><pages>e107642-e107642</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Vascular calcification, recapitulating bone formation, has a profound impact on plaque stability. The aim of the present study was to determine the influence of bone-like vascular calcification (named osteoid metaplasia = OM) and of osteoprotegerin on plaque stability.
Tissue from carotid endarterectomies were analysed for the presence of calcification and signs of vulnerability according to AHA grading system. Osteoprotegerin (OPG), pericytes and endothelial cells were sought using immuno-histochemistry. Symptoms and preoperative imaging findings (CT-scan, MRI and Doppler-scan) were analyzed. Human pericytes were cultured to evaluate their ability to secrete OPG and to influence mineralization in the plaque.
Seventy-three carotid plaques (49 asymptomatic and 24 symptomatic) were harvested. A significantly higher presence of OM (18.4% vs 0%, p<0.01), OPG (10.2% of ROI vs 3.4% of ROI, p<0.05) and pericytes (19% of ROI vs 3.8% of ROI, p<0.05) were noted in asymptomatic compared to symptomatic plaques. Consistently, circulating OPG levels were higher in the plasma of asymptomatic patients (3.2 ng/mL vs 2.5 ng/mL, p = 0.05). In vitro, human vascular pericytes secreted considerable amounts of OPG and underwent osteoblastic differentiation. Pericytes also inhibited the osteoclastic differentiation of CD14+ cells through their secretion of OPG.
OPG (intraplaque an plasmatic) and OM are associated with carotid plaque stability. Pericytes may be involved in the secretion of intraplaque OPG and in the formation of OM.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25259713</pmid><doi>10.1371/journal.pone.0107642</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1565690232 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Atherosclerosis Biocompatibility Biology and Life Sciences Biomedical materials Bone growth Bones Calcification Calcification (ectopic) Cardiovascular disease Carotid Stenosis - diagnosis Carotid Stenosis - metabolism Carotid Stenosis - pathology CD14 antigen CD146 Antigen - metabolism Cell Differentiation Computed tomography Cytokines Differentiation Endothelial cells Evaluation Family medical history Histochemistry Homeostasis Humans Ligands Magnetic resonance imaging Medicine and Health Sciences Metaplasia Mineralization Mortality Osteoblastogenesis Osteoblasts Osteoblasts - cytology Osteoblasts - metabolism Osteoclastogenesis Osteoclasts Osteogenesis Osteoid Osteoprotegerin Osteoprotegerin - blood Osteoprotegerin - metabolism Patients Pericytes Pericytes - cytology Pericytes - metabolism Plaque, Atherosclerotic - metabolism Plaque, Atherosclerotic - pathology Plaques RANK Ligand - metabolism Rodents Stability analysis Stroke Studies Surgery Tumor necrosis factor-TNF Vascular Calcification |
| title | Osteoprotegerin, pericytes and bone-like vascular calcification are associated with carotid plaque stability |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T04%3A27%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Osteoprotegerin,%20pericytes%20and%20bone-like%20vascular%20calcification%20are%20associated%20with%20carotid%20plaque%20stability&rft.jtitle=PloS%20one&rft.au=Davaine,%20Jean-Michel&rft.date=2014-09-26&rft.volume=9&rft.issue=9&rft.spage=e107642&rft.epage=e107642&rft.pages=e107642-e107642&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0107642&rft_dat=%3Cproquest_plos_%3E3444418051%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1565690232&rft_id=info:pmid/25259713&rft_doaj_id=oai_doaj_org_article_4b4a0f94d79845ea8e36fb623f213069&rfr_iscdi=true |