Semi-automated hydrophobic interaction chromatography column scouting used in the two-step purification of recombinant green fluorescent protein

Hydrophobic interaction chromatography (HIC) most commonly requires experimental determination (i.e., scouting) in order to select an optimal chromatographic medium for purifying a given target protein. Neither a two-step purification of untagged green fluorescent protein (GFP) from crude bacterial...

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Veröffentlicht in:	PloS one 2014-09, Vol.9 (9), p.e108611-e108611
Hauptverfasser:	Stone, Orrin J, Biette, Kelly M, Murphy, Patrick J M
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Ammonium phosphates Automation Bacteria Biochemistry Biology and Life Sciences Chromatography Chromatography - methods Chromatography, Gel Chromatography, Liquid E coli Elution Escherichia Fluorescence Fluorimetry Gel electrophoresis Green fluorescent protein Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - isolation & purification Health sciences Hydrophobic and Hydrophilic Interactions Hydrophobic surfaces Hydrophobicity Interdisciplinary aspects Laboratories Ligands Mathematical analysis Matrix methods Molecular biology Nursing pH effects Phosphates Protein purification Proteins Purification Recombinant Proteins Research and Analysis Methods Salts Science Policy Size exclusion chromatography Sodium lauryl sulfate Spectrophotometry
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