Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo
Genetically-encoded calcium indicators (GECIs) facilitate imaging activity of genetically defined neuronal populations in vivo. The high intracellular GECI concentrations required for in vivo imaging are usually achieved by viral gene transfer using adeno-associated viruses. Transgenic expression of...
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| Veröffentlicht in: | PloS one 2014-09, Vol.9 (9), p.e108697-e108697 |
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| creator | Dana, Hod Chen, Tsai-Wen Hu, Amy Shields, Brenda C Guo, Caiying Looger, Loren L Kim, Douglas S Svoboda, Karel |
| description | Genetically-encoded calcium indicators (GECIs) facilitate imaging activity of genetically defined neuronal populations in vivo. The high intracellular GECI concentrations required for in vivo imaging are usually achieved by viral gene transfer using adeno-associated viruses. Transgenic expression of GECIs promises important advantages, including homogeneous, repeatable, and stable expression without the need for invasive virus injections. Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. Furthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice. |
| doi_str_mv | 10.1371/journal.pone.0108697 |
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The high intracellular GECI concentrations required for in vivo imaging are usually achieved by viral gene transfer using adeno-associated viruses. Transgenic expression of GECIs promises important advantages, including homogeneous, repeatable, and stable expression without the need for invasive virus injections. Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. Furthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0108697</identifier><identifier>PMID: 25250714</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Behavior, Animal ; Biology and Life Sciences ; Brain ; Brain research ; Calcium ; Calcium - metabolism ; Calcium imaging ; Farm buildings ; Gene expression ; Gene therapy ; Gene transfer ; Hepatitis ; Imaging ; Laboratories ; Mice ; Mice, Transgenic ; Microscopy ; Neuroimaging ; Neurons ; Neurons - cytology ; Neurons - metabolism ; Neurosciences ; Population genetics ; Protein expression ; Proteins ; Rodents ; Transgenic animals ; Transgenic mice ; Viral infections ; Viruses ; Visual cortex ; Visual Cortex - cytology ; Visual Cortex - physiology</subject><ispartof>PloS one, 2014-09, Vol.9 (9), p.e108697-e108697</ispartof><rights>2014 Dana et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Dana et al 2014 Dana et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5077-dc020d982df78ebd9cc755ece71359c4aea2fdb7ec17946150a8c1a7350947c53</citedby><cites>FETCH-LOGICAL-c5077-dc020d982df78ebd9cc755ece71359c4aea2fdb7ec17946150a8c1a7350947c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177405/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177405/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25250714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Arenkiel, Benjamin</contributor><creatorcontrib>Dana, Hod</creatorcontrib><creatorcontrib>Chen, Tsai-Wen</creatorcontrib><creatorcontrib>Hu, Amy</creatorcontrib><creatorcontrib>Shields, Brenda C</creatorcontrib><creatorcontrib>Guo, Caiying</creatorcontrib><creatorcontrib>Looger, Loren L</creatorcontrib><creatorcontrib>Kim, Douglas S</creatorcontrib><creatorcontrib>Svoboda, Karel</creatorcontrib><title>Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Genetically-encoded calcium indicators (GECIs) facilitate imaging activity of genetically defined neuronal populations in vivo. The high intracellular GECI concentrations required for in vivo imaging are usually achieved by viral gene transfer using adeno-associated viruses. Transgenic expression of GECIs promises important advantages, including homogeneous, repeatable, and stable expression without the need for invasive virus injections. Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dana, Hod</au><au>Chen, Tsai-Wen</au><au>Hu, Amy</au><au>Shields, Brenda C</au><au>Guo, Caiying</au><au>Looger, Loren L</au><au>Kim, Douglas S</au><au>Svoboda, Karel</au><au>Arenkiel, Benjamin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-09-24</date><risdate>2014</risdate><volume>9</volume><issue>9</issue><spage>e108697</spage><epage>e108697</epage><pages>e108697-e108697</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Genetically-encoded calcium indicators (GECIs) facilitate imaging activity of genetically defined neuronal populations in vivo. The high intracellular GECI concentrations required for in vivo imaging are usually achieved by viral gene transfer using adeno-associated viruses. Transgenic expression of GECIs promises important advantages, including homogeneous, repeatable, and stable expression without the need for invasive virus injections. Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. Furthermore, we show that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25250714</pmid><doi>10.1371/journal.pone.0108697</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Behavior, Animal Biology and Life Sciences Brain Brain research Calcium Calcium - metabolism Calcium imaging Farm buildings Gene expression Gene therapy Gene transfer Hepatitis Imaging Laboratories Mice Mice, Transgenic Microscopy Neuroimaging Neurons Neurons - cytology Neurons - metabolism Neurosciences Population genetics Protein expression Proteins Rodents Transgenic animals Transgenic mice Viral infections Viruses Visual cortex Visual Cortex - cytology Visual Cortex - physiology |
| title | Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo |
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