Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix
Vacuum-assisted closure (VAC) negative pressure wound therapy (NPWT) has been proven to be an effective therapeutic method for the treatment of recalcitrant wounds. However, its role in bone healing remains to be unclear. Here, we investigated the effects of NPWT on rat periosteum-derived mesenchyma...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-09, Vol.9 (9), p.e107339-e107339 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e107339 |
|---|---|
| container_issue | 9 |
| container_start_page | e107339 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Zhu, Jin Yu, Aixi Qi, Baiwen Li, Zonghuan Hu, Xiang |
| description | Vacuum-assisted closure (VAC) negative pressure wound therapy (NPWT) has been proven to be an effective therapeutic method for the treatment of recalcitrant wounds. However, its role in bone healing remains to be unclear. Here, we investigated the effects of NPWT on rat periosteum-derived mesenchymal stem cells (P-MSCs) proliferation and osteoblastic differentiation in a 3D fibrin matrix. P-MSCs underwent primary culture for three passages before being used to construct cell clots. The fibrin clots were incubated with NPWT under continuous suction at -125 mmHg in a subatmospheric perfusion bioreactor. Clots exposed to atmospheric pressure served as the static control. Compared to the control group, cell proliferation significantly increased in NPWT group after incubation for 3 days. There was no statistical difference in apoptosis rate between two groups. The ALP activity and mineralization of P-MSCs all increased under continuous suction. The expressions of collagen type 1 and transcription factor Cbfa-1 were higher at the 1-, 3-, and 7-day timepoints and the expressions of osteocalcin and integrin β5 were higher at the 3-, and 7-day timepoints in the NPWT group. These results indicate that a short time treatment with NPWT, applied with continuous suction at -125 mmHg, can enhance cellular proliferation of P-MSCs and induce the differentiation toward an osteogenic phenotype. The mechanotransduction molecule integrin β5 was found to be highly expressed after NPWT treatment, which indicates that NPWT may play a positive role in fracture healing through enhance bone formation and decrease bone resorption. |
| doi_str_mv | 10.1371/journal.pone.0107339 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1561634702</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_feb9d4519f91446594a2208faa57624c</doaj_id><sourcerecordid>3430848581</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-5e366c243b40c843fda3508e375d242848c56a679f0b0dbc95d26075d0c32a143</originalsourceid><addsrcrecordid>eNptUk1v1DAQjRCIlsI_QGCJC5dd_J3kgoSqApUqcYGz5TjjXa8Se7Gdwt77w-t006pFnDzye2_mzehV1VuC14TV5NMuTNHrYb0PHtaY4Jqx9ll1SlpGV5Ji9vxRfVK9SmmHsWCNlC-rEyookaShp9XNhbVgckLBIg8bnd01oH2ElKYI6E-YfI_yFqLeH1DwaIQE3mwPox5QyjAiA8OQiiAMzhZWdoWkiyYUNGzAO4N6V0ZE8NkdYVcYyLoulmLUObq_r6sXVg8J3izvWfXr68XP8--rqx_fLs-_XK2MoDKvBDApDeWs49g0nNleM4EbYLXoKacNb4yQWtatxR3uO9OWb4kLiA2jmnB2Vr0_9t0PIanlgEkRIYlkvMa0MC6PjD7ondpHN-p4UEE7dfcR4kbpmJ0ZQFno2p4L0tqWcC5FyzWluLFai1pSbkqvz8u0qRuhN-UCUQ9Pmj5FvNuqTbhWnEgqmtnux6VBDL8nSFmNLs0H1x7CdOe7rF1zOfv-8A_1_9vxI8vEkFIE-2CGYDWH6l6l5lCpJVRF9u7xIg-i-xSxW5ZzzK4</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1561634702</pqid></control><display><type>article</type><title>Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Zhu, Jin ; Yu, Aixi ; Qi, Baiwen ; Li, Zonghuan ; Hu, Xiang</creator><contributor>Matsumoto, Takuya</contributor><creatorcontrib>Zhu, Jin ; Yu, Aixi ; Qi, Baiwen ; Li, Zonghuan ; Hu, Xiang ; Matsumoto, Takuya</creatorcontrib><description>Vacuum-assisted closure (VAC) negative pressure wound therapy (NPWT) has been proven to be an effective therapeutic method for the treatment of recalcitrant wounds. However, its role in bone healing remains to be unclear. Here, we investigated the effects of NPWT on rat periosteum-derived mesenchymal stem cells (P-MSCs) proliferation and osteoblastic differentiation in a 3D fibrin matrix. P-MSCs underwent primary culture for three passages before being used to construct cell clots. The fibrin clots were incubated with NPWT under continuous suction at -125 mmHg in a subatmospheric perfusion bioreactor. Clots exposed to atmospheric pressure served as the static control. Compared to the control group, cell proliferation significantly increased in NPWT group after incubation for 3 days. There was no statistical difference in apoptosis rate between two groups. The ALP activity and mineralization of P-MSCs all increased under continuous suction. The expressions of collagen type 1 and transcription factor Cbfa-1 were higher at the 1-, 3-, and 7-day timepoints and the expressions of osteocalcin and integrin β5 were higher at the 3-, and 7-day timepoints in the NPWT group. These results indicate that a short time treatment with NPWT, applied with continuous suction at -125 mmHg, can enhance cellular proliferation of P-MSCs and induce the differentiation toward an osteogenic phenotype. The mechanotransduction molecule integrin β5 was found to be highly expressed after NPWT treatment, which indicates that NPWT may play a positive role in fracture healing through enhance bone formation and decrease bone resorption.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0107339</identifier><identifier>PMID: 25216182</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Apoptosis ; Atmospheric pressure ; Biocompatibility ; Biology and Life Sciences ; Bioreactors ; Bone growth ; Bone healing ; Bone marrow ; Bone resorption ; Cbfa-1 protein ; Cell culture ; Cell Differentiation - physiology ; Cell Line ; Cell proliferation ; Cell Proliferation - physiology ; Collagen (type I) ; Differentiation (biology) ; Fibrin ; Fractures ; Gene Expression ; Healing ; Humans ; Integrin beta Chains - biosynthesis ; Mechanotransduction ; Medicine and Health Sciences ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - physiology ; Mesenchyme ; Mineralization ; Molecular chains ; Negative-Pressure Wound Therapy ; Orthopedics ; Osteoblastogenesis ; Osteoblasts ; Osteocalcin ; Osteogenesis ; Osteogenesis - physiology ; Perfusion ; Periosteum ; Phenotypes ; Pressure effects ; Pressure ulcers ; Rats ; Rodents ; Stem cell transplantation ; Stem cells ; Suction ; Therapy ; Transcription factors ; Wound healing ; Wounds</subject><ispartof>PloS one, 2014-09, Vol.9 (9), p.e107339-e107339</ispartof><rights>2014 Zhu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Zhu et al 2014 Zhu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-5e366c243b40c843fda3508e375d242848c56a679f0b0dbc95d26075d0c32a143</citedby><cites>FETCH-LOGICAL-c526t-5e366c243b40c843fda3508e375d242848c56a679f0b0dbc95d26075d0c32a143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162584/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4162584/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25216182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Matsumoto, Takuya</contributor><creatorcontrib>Zhu, Jin</creatorcontrib><creatorcontrib>Yu, Aixi</creatorcontrib><creatorcontrib>Qi, Baiwen</creatorcontrib><creatorcontrib>Li, Zonghuan</creatorcontrib><creatorcontrib>Hu, Xiang</creatorcontrib><title>Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Vacuum-assisted closure (VAC) negative pressure wound therapy (NPWT) has been proven to be an effective therapeutic method for the treatment of recalcitrant wounds. However, its role in bone healing remains to be unclear. Here, we investigated the effects of NPWT on rat periosteum-derived mesenchymal stem cells (P-MSCs) proliferation and osteoblastic differentiation in a 3D fibrin matrix. P-MSCs underwent primary culture for three passages before being used to construct cell clots. The fibrin clots were incubated with NPWT under continuous suction at -125 mmHg in a subatmospheric perfusion bioreactor. Clots exposed to atmospheric pressure served as the static control. Compared to the control group, cell proliferation significantly increased in NPWT group after incubation for 3 days. There was no statistical difference in apoptosis rate between two groups. The ALP activity and mineralization of P-MSCs all increased under continuous suction. The expressions of collagen type 1 and transcription factor Cbfa-1 were higher at the 1-, 3-, and 7-day timepoints and the expressions of osteocalcin and integrin β5 were higher at the 3-, and 7-day timepoints in the NPWT group. These results indicate that a short time treatment with NPWT, applied with continuous suction at -125 mmHg, can enhance cellular proliferation of P-MSCs and induce the differentiation toward an osteogenic phenotype. The mechanotransduction molecule integrin β5 was found to be highly expressed after NPWT treatment, which indicates that NPWT may play a positive role in fracture healing through enhance bone formation and decrease bone resorption.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Atmospheric pressure</subject><subject>Biocompatibility</subject><subject>Biology and Life Sciences</subject><subject>Bioreactors</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone marrow</subject><subject>Bone resorption</subject><subject>Cbfa-1 protein</subject><subject>Cell culture</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - physiology</subject><subject>Collagen (type I)</subject><subject>Differentiation (biology)</subject><subject>Fibrin</subject><subject>Fractures</subject><subject>Gene Expression</subject><subject>Healing</subject><subject>Humans</subject><subject>Integrin beta Chains - biosynthesis</subject><subject>Mechanotransduction</subject><subject>Medicine and Health Sciences</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Mesenchyme</subject><subject>Mineralization</subject><subject>Molecular chains</subject><subject>Negative-Pressure Wound Therapy</subject><subject>Orthopedics</subject><subject>Osteoblastogenesis</subject><subject>Osteoblasts</subject><subject>Osteocalcin</subject><subject>Osteogenesis</subject><subject>Osteogenesis - physiology</subject><subject>Perfusion</subject><subject>Periosteum</subject><subject>Phenotypes</subject><subject>Pressure effects</subject><subject>Pressure ulcers</subject><subject>Rats</subject><subject>Rodents</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Suction</subject><subject>Therapy</subject><subject>Transcription factors</subject><subject>Wound healing</subject><subject>Wounds</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1v1DAQjRCIlsI_QGCJC5dd_J3kgoSqApUqcYGz5TjjXa8Se7Gdwt77w-t006pFnDzye2_mzehV1VuC14TV5NMuTNHrYb0PHtaY4Jqx9ll1SlpGV5Ji9vxRfVK9SmmHsWCNlC-rEyookaShp9XNhbVgckLBIg8bnd01oH2ElKYI6E-YfI_yFqLeH1DwaIQE3mwPox5QyjAiA8OQiiAMzhZWdoWkiyYUNGzAO4N6V0ZE8NkdYVcYyLoulmLUObq_r6sXVg8J3izvWfXr68XP8--rqx_fLs-_XK2MoDKvBDApDeWs49g0nNleM4EbYLXoKacNb4yQWtatxR3uO9OWb4kLiA2jmnB2Vr0_9t0PIanlgEkRIYlkvMa0MC6PjD7ondpHN-p4UEE7dfcR4kbpmJ0ZQFno2p4L0tqWcC5FyzWluLFai1pSbkqvz8u0qRuhN-UCUQ9Pmj5FvNuqTbhWnEgqmtnux6VBDL8nSFmNLs0H1x7CdOe7rF1zOfv-8A_1_9vxI8vEkFIE-2CGYDWH6l6l5lCpJVRF9u7xIg-i-xSxW5ZzzK4</recordid><startdate>20140912</startdate><enddate>20140912</enddate><creator>Zhu, Jin</creator><creator>Yu, Aixi</creator><creator>Qi, Baiwen</creator><creator>Li, Zonghuan</creator><creator>Hu, Xiang</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140912</creationdate><title>Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix</title><author>Zhu, Jin ; Yu, Aixi ; Qi, Baiwen ; Li, Zonghuan ; Hu, Xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-5e366c243b40c843fda3508e375d242848c56a679f0b0dbc95d26075d0c32a143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Atmospheric pressure</topic><topic>Biocompatibility</topic><topic>Biology and Life Sciences</topic><topic>Bioreactors</topic><topic>Bone growth</topic><topic>Bone healing</topic><topic>Bone marrow</topic><topic>Bone resorption</topic><topic>Cbfa-1 protein</topic><topic>Cell culture</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - physiology</topic><topic>Collagen (type I)</topic><topic>Differentiation (biology)</topic><topic>Fibrin</topic><topic>Fractures</topic><topic>Gene Expression</topic><topic>Healing</topic><topic>Humans</topic><topic>Integrin beta Chains - biosynthesis</topic><topic>Mechanotransduction</topic><topic>Medicine and Health Sciences</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Mesenchyme</topic><topic>Mineralization</topic><topic>Molecular chains</topic><topic>Negative-Pressure Wound Therapy</topic><topic>Orthopedics</topic><topic>Osteoblastogenesis</topic><topic>Osteoblasts</topic><topic>Osteocalcin</topic><topic>Osteogenesis</topic><topic>Osteogenesis - physiology</topic><topic>Perfusion</topic><topic>Periosteum</topic><topic>Phenotypes</topic><topic>Pressure effects</topic><topic>Pressure ulcers</topic><topic>Rats</topic><topic>Rodents</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Suction</topic><topic>Therapy</topic><topic>Transcription factors</topic><topic>Wound healing</topic><topic>Wounds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Jin</creatorcontrib><creatorcontrib>Yu, Aixi</creatorcontrib><creatorcontrib>Qi, Baiwen</creatorcontrib><creatorcontrib>Li, Zonghuan</creatorcontrib><creatorcontrib>Hu, Xiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Jin</au><au>Yu, Aixi</au><au>Qi, Baiwen</au><au>Li, Zonghuan</au><au>Hu, Xiang</au><au>Matsumoto, Takuya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-09-12</date><risdate>2014</risdate><volume>9</volume><issue>9</issue><spage>e107339</spage><epage>e107339</epage><pages>e107339-e107339</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Vacuum-assisted closure (VAC) negative pressure wound therapy (NPWT) has been proven to be an effective therapeutic method for the treatment of recalcitrant wounds. However, its role in bone healing remains to be unclear. Here, we investigated the effects of NPWT on rat periosteum-derived mesenchymal stem cells (P-MSCs) proliferation and osteoblastic differentiation in a 3D fibrin matrix. P-MSCs underwent primary culture for three passages before being used to construct cell clots. The fibrin clots were incubated with NPWT under continuous suction at -125 mmHg in a subatmospheric perfusion bioreactor. Clots exposed to atmospheric pressure served as the static control. Compared to the control group, cell proliferation significantly increased in NPWT group after incubation for 3 days. There was no statistical difference in apoptosis rate between two groups. The ALP activity and mineralization of P-MSCs all increased under continuous suction. The expressions of collagen type 1 and transcription factor Cbfa-1 were higher at the 1-, 3-, and 7-day timepoints and the expressions of osteocalcin and integrin β5 were higher at the 3-, and 7-day timepoints in the NPWT group. These results indicate that a short time treatment with NPWT, applied with continuous suction at -125 mmHg, can enhance cellular proliferation of P-MSCs and induce the differentiation toward an osteogenic phenotype. The mechanotransduction molecule integrin β5 was found to be highly expressed after NPWT treatment, which indicates that NPWT may play a positive role in fracture healing through enhance bone formation and decrease bone resorption.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25216182</pmid><doi>10.1371/journal.pone.0107339</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-09, Vol.9 (9), p.e107339-e107339 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1561634702 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Apoptosis Atmospheric pressure Biocompatibility Biology and Life Sciences Bioreactors Bone growth Bone healing Bone marrow Bone resorption Cbfa-1 protein Cell culture Cell Differentiation - physiology Cell Line Cell proliferation Cell Proliferation - physiology Collagen (type I) Differentiation (biology) Fibrin Fractures Gene Expression Healing Humans Integrin beta Chains - biosynthesis Mechanotransduction Medicine and Health Sciences Mesenchymal stem cells Mesenchymal Stromal Cells - physiology Mesenchyme Mineralization Molecular chains Negative-Pressure Wound Therapy Orthopedics Osteoblastogenesis Osteoblasts Osteocalcin Osteogenesis Osteogenesis - physiology Perfusion Periosteum Phenotypes Pressure effects Pressure ulcers Rats Rodents Stem cell transplantation Stem cells Suction Therapy Transcription factors Wound healing Wounds |
| title | Effects of negative pressure wound therapy on mesenchymal stem cells proliferation and osteogenic differentiation in a fibrin matrix |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T23%3A34%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20negative%20pressure%20wound%20therapy%20on%20mesenchymal%20stem%20cells%20proliferation%20and%20osteogenic%20differentiation%20in%20a%20fibrin%20matrix&rft.jtitle=PloS%20one&rft.au=Zhu,%20Jin&rft.date=2014-09-12&rft.volume=9&rft.issue=9&rft.spage=e107339&rft.epage=e107339&rft.pages=e107339-e107339&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0107339&rft_dat=%3Cproquest_plos_%3E3430848581%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1561634702&rft_id=info:pmid/25216182&rft_doaj_id=oai_doaj_org_article_feb9d4519f91446594a2208faa57624c&rfr_iscdi=true |