Mycobacterium tuberculosis DosR is required for activity of the PmbtB and PmbtI promoters under hypoxia
Mycobacterium tuberculosis has the ability to survive for extended periods of time under conditions of low oxygen, low pH, low iron and low nutrients. The mycobactins (M. tuberculosis siderophores) play a key role in scavenging iron from the environment and are induced in response to low iron in an...
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| Veröffentlicht in: | PloS one 2014-09, Vol.9 (9), p.e107283-e107283 |
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| description | Mycobacterium tuberculosis has the ability to survive for extended periods of time under conditions of low oxygen, low pH, low iron and low nutrients. The mycobactins (M. tuberculosis siderophores) play a key role in scavenging iron from the environment and are induced in response to low iron in an IdeR-regulated manner. We demonstrate that the promoters of two mycobactin gene (mbt) operons are also expressed during adaptation to low oxygen, and that this expression is dependent on the DosR regulator. Up-regulation of mbt operons induced by low iron was not DosR-dependent. DosR is a member of a two component regulatory system which responds to oxygen availability. Deletion of the DosR regulator led to increased expression of bacterioferritin and increased capacity to grow under iron depletion. These data provide a link between the mycobacterial response to two conditions likely to be encountered in vivo, low iron and low oxygen. |
| doi_str_mv | 10.1371/journal.pone.0107283 |
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The mycobactins (M. tuberculosis siderophores) play a key role in scavenging iron from the environment and are induced in response to low iron in an IdeR-regulated manner. We demonstrate that the promoters of two mycobactin gene (mbt) operons are also expressed during adaptation to low oxygen, and that this expression is dependent on the DosR regulator. Up-regulation of mbt operons induced by low iron was not DosR-dependent. DosR is a member of a two component regulatory system which responds to oxygen availability. Deletion of the DosR regulator led to increased expression of bacterioferritin and increased capacity to grow under iron depletion. These data provide a link between the mycobacterial response to two conditions likely to be encountered in vivo, low iron and low oxygen.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0107283</identifier><identifier>PMID: 25211224</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptation ; Adaptation, Physiological ; Bacterial Proteins - physiology ; Bacterioferritin ; Biology and Life Sciences ; Dentistry ; DNA-Binding Proteins ; Gene Expression ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Hypoxia ; Infections ; Iron ; Iron - metabolism ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - metabolism ; Nutrients ; Operon ; Operons ; Oxygen ; Oxygen - metabolism ; Promoter Regions, Genetic ; Promoters ; Protein Kinases - physiology ; Siderophores ; Tuberculosis</subject><ispartof>PloS one, 2014-09, Vol.9 (9), p.e107283-e107283</ispartof><rights>2014 Schreuder, Parish. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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The mycobactins (M. tuberculosis siderophores) play a key role in scavenging iron from the environment and are induced in response to low iron in an IdeR-regulated manner. We demonstrate that the promoters of two mycobactin gene (mbt) operons are also expressed during adaptation to low oxygen, and that this expression is dependent on the DosR regulator. Up-regulation of mbt operons induced by low iron was not DosR-dependent. DosR is a member of a two component regulatory system which responds to oxygen availability. Deletion of the DosR regulator led to increased expression of bacterioferritin and increased capacity to grow under iron depletion. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schreuder, Lise J</au><au>Parish, Tanya</au><au>Cardona, Pere-Joan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mycobacterium tuberculosis DosR is required for activity of the PmbtB and PmbtI promoters under hypoxia</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>9</volume><issue>9</issue><spage>e107283</spage><epage>e107283</epage><pages>e107283-e107283</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mycobacterium tuberculosis has the ability to survive for extended periods of time under conditions of low oxygen, low pH, low iron and low nutrients. 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| subjects | Adaptation Adaptation, Physiological Bacterial Proteins - physiology Bacterioferritin Biology and Life Sciences Dentistry DNA-Binding Proteins Gene Expression Gene Expression Regulation, Bacterial Genes, Bacterial Hypoxia Infections Iron Iron - metabolism Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - metabolism Nutrients Operon Operons Oxygen Oxygen - metabolism Promoter Regions, Genetic Promoters Protein Kinases - physiology Siderophores Tuberculosis |
| title | Mycobacterium tuberculosis DosR is required for activity of the PmbtB and PmbtI promoters under hypoxia |
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