Electron tomography of cryo-immobilized plant tissue: a novel approach to studying 3D macromolecular architecture of mature plant cell walls in situ

Cost-effective production of lignocellulosic biofuel requires efficient breakdown of cell walls present in plant biomass to retrieve the wall polysaccharides for fermentation. In-depth knowledge of plant cell wall composition is therefore essential for improving the fuel production process. The prec...

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Veröffentlicht in:	PloS one 2014-09, Vol.9 (9), p.e106928-e106928
Hauptverfasser:	Sarkar, Purbasha, Bosneaga, Elena, Yap, Jr, Edgar G, Das, Jyotirmoy, Tsai, Wen-Ting, Cabal, Angelo, Neuhaus, Erica, Maji, Dolonchampa, Kumar, Shailabh, Joo, Michael, Yakovlev, Sergey, Csencsits, Roseann, Yu, Zeyun, Bajaj, Chandrajit, Downing, Kenneth H, Auer, Manfred
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis - cytology Arabidopsis - genetics Arabidopsis - metabolism arabidopsis thaliana Architecture BASIC BIOLOGICAL SCIENCES Biofuels Biology and Life Sciences Biomass capillary tubes Cell culture Cell Wall - genetics Cell Wall - metabolism Cell Wall - ultrastructure Cell walls Cells, Immobilized - ultrastructure Cellulose Cellulose - metabolism Chemical extraction Cryopreservation Cryoultramicrotomy Deconstruction Electron Microscope Tomography - methods Electrons Embedding Energy Engineering and Technology Enzymes Fermentation Freeze Substitution Fuel production Glycoproteins Hemicellulose Hydrogen bonds Image resolution Imaging, Three-Dimensional - methods Laboratories Life sciences Lignin Lignocellulose Macromolecules Microfibrils Microscopy Mutation Parenchyma Pectin Plant biomass plant cells walls Plant tissues Polysaccharides primary cells Qualitative analysis Research and Analysis Methods Saccharides secondary cells Sectioning Stain Temperature Three dimensional models Tissue Embedding Tomography Walls
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