Atherosclerosis-related circulating miRNAs as novel and sensitive predictors for acute myocardial infarction
The dysregulated expressions of circulating miRNAs have been detected in various cardiovascular diseases. In our previous experiments, the altered expressions of circulating miRNA-21-5p, miRNA-361-5p and miRNA-519e-5p were confirmed in patients with coronary atherosclerosis by miRNA microarrays. How...
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| description | The dysregulated expressions of circulating miRNAs have been detected in various cardiovascular diseases. In our previous experiments, the altered expressions of circulating miRNA-21-5p, miRNA-361-5p and miRNA-519e-5p were confirmed in patients with coronary atherosclerosis by miRNA microarrays. However, the expression levels of these circulating miRNAs in the early phase of acute myocardial infarction (AMI) are still unknown. In the present study, our aims were to examine the expressions of circulating miR-21-5p, miR-361-5p and miR-519e-5p in AMI patients, and assess their clinical applications for diagnosing and monitoring AMI.
Two different cohorts were enrolled in this study. The first cohort included 17 AMI patients and 28 healthy volunteers, and the second cohort included 9 AMI patients, 9 ischemic stroke patients, 8 patients with pulmonary embolism, and 12 healthy volunteers. Quantitative real-time PCR and ELISA assays were preformed to detect the concentrations of plasma miRNAs and cardiac troponin I (cTnI), respectively. The results showed that the plasma levels of miR-21-5p and miR-361-5p were significantly increased in AMI patients, whereas the concentration of circulating miR-519e-5p was reduced. Interestingly, the levels of these circulating miRNAs correlated with the concentrations of plasma cTnI. Receiver operating characteristic (ROC) analysis revealed that these three circulating miRNAs had considerable diagnostic accuracy for AMI with high values of area under ROC curve (AUC). Importantly, combining the three miRNAs significantly increased the diagnostic accuracy. Furthermore, cell experiments demonstrated that these plasma miRNAs may originate from injured cardiomyocytes induced by hypoxia. In addition, the levels of all the three circulating miRNAs in ischemic stroke (IS) and pulmonary embolism (PE) were elevated, whereas the decreased level of plasma miR-519e-5p was only detected in AMI. ROC analysis demonstrated that circulating miR-519e-5p may be a useful biomarker for distinguishing AMI from other ischemic diseases.
Circulating miRNAs may be novel and powerful biomarkers for AMI and they could be potential diagnostic tool for AMI. |
| doi_str_mv | 10.1371/journal.pone.0105734 |
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Two different cohorts were enrolled in this study. The first cohort included 17 AMI patients and 28 healthy volunteers, and the second cohort included 9 AMI patients, 9 ischemic stroke patients, 8 patients with pulmonary embolism, and 12 healthy volunteers. Quantitative real-time PCR and ELISA assays were preformed to detect the concentrations of plasma miRNAs and cardiac troponin I (cTnI), respectively. The results showed that the plasma levels of miR-21-5p and miR-361-5p were significantly increased in AMI patients, whereas the concentration of circulating miR-519e-5p was reduced. Interestingly, the levels of these circulating miRNAs correlated with the concentrations of plasma cTnI. Receiver operating characteristic (ROC) analysis revealed that these three circulating miRNAs had considerable diagnostic accuracy for AMI with high values of area under ROC curve (AUC). Importantly, combining the three miRNAs significantly increased the diagnostic accuracy. Furthermore, cell experiments demonstrated that these plasma miRNAs may originate from injured cardiomyocytes induced by hypoxia. In addition, the levels of all the three circulating miRNAs in ischemic stroke (IS) and pulmonary embolism (PE) were elevated, whereas the decreased level of plasma miR-519e-5p was only detected in AMI. ROC analysis demonstrated that circulating miR-519e-5p may be a useful biomarker for distinguishing AMI from other ischemic diseases.
Circulating miRNAs may be novel and powerful biomarkers for AMI and they could be potential diagnostic tool for AMI.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0105734</identifier><identifier>PMID: 25184815</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Anticoagulants ; Area Under Curve ; Arteriosclerosis ; Atherosclerosis ; Bioindicators ; Biomarkers ; Biomarkers - blood ; Brain Ischemia - blood ; Brain Ischemia - diagnosis ; Brain Ischemia - genetics ; Calcium-binding protein ; Cardiomyocytes ; Cardiovascular disease ; Cardiovascular diseases ; Case-Control Studies ; Cell adhesion & migration ; Cell Hypoxia ; Cerebral infarction ; Diagnostic software ; Diagnostic systems ; Electrocardiography ; Embolism ; Embolisms ; Enzyme-linked immunosorbent assay ; Female ; Gene expression ; Heart attacks ; Heart diseases ; Hospitals ; Humans ; Hypertension ; Hypoxia ; Infarction ; Internal medicine ; Ischemia ; Kinases ; Male ; Medicine ; Medicine and Health Sciences ; MicroRNAs ; MicroRNAs - blood ; MicroRNAs - genetics ; Middle Aged ; miRNA ; Mortality ; Myocardial infarction ; Myocardial Infarction - blood ; Myocardial Infarction - diagnosis ; Myocardial Infarction - genetics ; Myocytes, Cardiac - metabolism ; Myocytes, Cardiac - pathology ; Patients ; Plasma ; Plasma levels ; Prostate cancer ; Pulmonary Embolism - blood ; Pulmonary Embolism - diagnosis ; Pulmonary Embolism - genetics ; ROC Curve ; Science ; Stroke ; Stroke - blood ; Stroke - diagnosis ; Stroke - genetics ; Therapeutic applications ; Troponin ; Troponin I ; Troponin I - blood ; Troponin I - genetics</subject><ispartof>PloS one, 2014-09, Vol.9 (9), p.e105734</ispartof><rights>2014 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Wang et al 2014 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-5ca32d7fcbd1611b2896d6fc51db03a9245b61730b2aa505101322db2642dc053</citedby><cites>FETCH-LOGICAL-c592t-5ca32d7fcbd1611b2896d6fc51db03a9245b61730b2aa505101322db2642dc053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153586/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4153586/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25184815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Zirlik, Andreas</contributor><creatorcontrib>Wang, Feng</creatorcontrib><creatorcontrib>Long, Guangwen</creatorcontrib><creatorcontrib>Zhao, Chunxia</creatorcontrib><creatorcontrib>Li, Huaping</creatorcontrib><creatorcontrib>Chaugai, Sandip</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Chen, Chen</creatorcontrib><creatorcontrib>Wang, Dao Wen</creatorcontrib><title>Atherosclerosis-related circulating miRNAs as novel and sensitive predictors for acute myocardial infarction</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The dysregulated expressions of circulating miRNAs have been detected in various cardiovascular diseases. In our previous experiments, the altered expressions of circulating miRNA-21-5p, miRNA-361-5p and miRNA-519e-5p were confirmed in patients with coronary atherosclerosis by miRNA microarrays. However, the expression levels of these circulating miRNAs in the early phase of acute myocardial infarction (AMI) are still unknown. In the present study, our aims were to examine the expressions of circulating miR-21-5p, miR-361-5p and miR-519e-5p in AMI patients, and assess their clinical applications for diagnosing and monitoring AMI.
Two different cohorts were enrolled in this study. The first cohort included 17 AMI patients and 28 healthy volunteers, and the second cohort included 9 AMI patients, 9 ischemic stroke patients, 8 patients with pulmonary embolism, and 12 healthy volunteers. Quantitative real-time PCR and ELISA assays were preformed to detect the concentrations of plasma miRNAs and cardiac troponin I (cTnI), respectively. The results showed that the plasma levels of miR-21-5p and miR-361-5p were significantly increased in AMI patients, whereas the concentration of circulating miR-519e-5p was reduced. Interestingly, the levels of these circulating miRNAs correlated with the concentrations of plasma cTnI. Receiver operating characteristic (ROC) analysis revealed that these three circulating miRNAs had considerable diagnostic accuracy for AMI with high values of area under ROC curve (AUC). Importantly, combining the three miRNAs significantly increased the diagnostic accuracy. Furthermore, cell experiments demonstrated that these plasma miRNAs may originate from injured cardiomyocytes induced by hypoxia. In addition, the levels of all the three circulating miRNAs in ischemic stroke (IS) and pulmonary embolism (PE) were elevated, whereas the decreased level of plasma miR-519e-5p was only detected in AMI. ROC analysis demonstrated that circulating miR-519e-5p may be a useful biomarker for distinguishing AMI from other ischemic diseases.
Circulating miRNAs may be novel and powerful biomarkers for AMI and they could be potential diagnostic tool for AMI.</description><subject>Adult</subject><subject>Aged</subject><subject>Anticoagulants</subject><subject>Area Under Curve</subject><subject>Arteriosclerosis</subject><subject>Atherosclerosis</subject><subject>Bioindicators</subject><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>Brain Ischemia - blood</subject><subject>Brain Ischemia - diagnosis</subject><subject>Brain Ischemia - genetics</subject><subject>Calcium-binding protein</subject><subject>Cardiomyocytes</subject><subject>Cardiovascular disease</subject><subject>Cardiovascular diseases</subject><subject>Case-Control Studies</subject><subject>Cell adhesion & migration</subject><subject>Cell Hypoxia</subject><subject>Cerebral infarction</subject><subject>Diagnostic software</subject><subject>Diagnostic systems</subject><subject>Electrocardiography</subject><subject>Embolism</subject><subject>Embolisms</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Gene expression</subject><subject>Heart attacks</subject><subject>Heart diseases</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Hypertension</subject><subject>Hypoxia</subject><subject>Infarction</subject><subject>Internal medicine</subject><subject>Ischemia</subject><subject>Kinases</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>MicroRNAs</subject><subject>MicroRNAs - blood</subject><subject>MicroRNAs - genetics</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>Mortality</subject><subject>Myocardial infarction</subject><subject>Myocardial Infarction - blood</subject><subject>Myocardial Infarction - diagnosis</subject><subject>Myocardial Infarction - genetics</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Myocytes, Cardiac - pathology</subject><subject>Patients</subject><subject>Plasma</subject><subject>Plasma levels</subject><subject>Prostate cancer</subject><subject>Pulmonary Embolism - blood</subject><subject>Pulmonary Embolism - diagnosis</subject><subject>Pulmonary Embolism - genetics</subject><subject>ROC Curve</subject><subject>Science</subject><subject>Stroke</subject><subject>Stroke - blood</subject><subject>Stroke - diagnosis</subject><subject>Stroke - genetics</subject><subject>Therapeutic applications</subject><subject>Troponin</subject><subject>Troponin I</subject><subject>Troponin I - blood</subject><subject>Troponin I - 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blood</topic><topic>MicroRNAs - genetics</topic><topic>Middle Aged</topic><topic>miRNA</topic><topic>Mortality</topic><topic>Myocardial infarction</topic><topic>Myocardial Infarction - blood</topic><topic>Myocardial Infarction - diagnosis</topic><topic>Myocardial Infarction - genetics</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Myocytes, Cardiac - pathology</topic><topic>Patients</topic><topic>Plasma</topic><topic>Plasma levels</topic><topic>Prostate cancer</topic><topic>Pulmonary Embolism - blood</topic><topic>Pulmonary Embolism - diagnosis</topic><topic>Pulmonary Embolism - genetics</topic><topic>ROC Curve</topic><topic>Science</topic><topic>Stroke</topic><topic>Stroke - blood</topic><topic>Stroke - diagnosis</topic><topic>Stroke - genetics</topic><topic>Therapeutic applications</topic><topic>Troponin</topic><topic>Troponin I</topic><topic>Troponin I - blood</topic><topic>Troponin I - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Feng</creatorcontrib><creatorcontrib>Long, Guangwen</creatorcontrib><creatorcontrib>Zhao, Chunxia</creatorcontrib><creatorcontrib>Li, Huaping</creatorcontrib><creatorcontrib>Chaugai, Sandip</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Chen, Chen</creatorcontrib><creatorcontrib>Wang, Dao Wen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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In our previous experiments, the altered expressions of circulating miRNA-21-5p, miRNA-361-5p and miRNA-519e-5p were confirmed in patients with coronary atherosclerosis by miRNA microarrays. However, the expression levels of these circulating miRNAs in the early phase of acute myocardial infarction (AMI) are still unknown. In the present study, our aims were to examine the expressions of circulating miR-21-5p, miR-361-5p and miR-519e-5p in AMI patients, and assess their clinical applications for diagnosing and monitoring AMI.
Two different cohorts were enrolled in this study. The first cohort included 17 AMI patients and 28 healthy volunteers, and the second cohort included 9 AMI patients, 9 ischemic stroke patients, 8 patients with pulmonary embolism, and 12 healthy volunteers. Quantitative real-time PCR and ELISA assays were preformed to detect the concentrations of plasma miRNAs and cardiac troponin I (cTnI), respectively. The results showed that the plasma levels of miR-21-5p and miR-361-5p were significantly increased in AMI patients, whereas the concentration of circulating miR-519e-5p was reduced. Interestingly, the levels of these circulating miRNAs correlated with the concentrations of plasma cTnI. Receiver operating characteristic (ROC) analysis revealed that these three circulating miRNAs had considerable diagnostic accuracy for AMI with high values of area under ROC curve (AUC). Importantly, combining the three miRNAs significantly increased the diagnostic accuracy. Furthermore, cell experiments demonstrated that these plasma miRNAs may originate from injured cardiomyocytes induced by hypoxia. In addition, the levels of all the three circulating miRNAs in ischemic stroke (IS) and pulmonary embolism (PE) were elevated, whereas the decreased level of plasma miR-519e-5p was only detected in AMI. ROC analysis demonstrated that circulating miR-519e-5p may be a useful biomarker for distinguishing AMI from other ischemic diseases.
Circulating miRNAs may be novel and powerful biomarkers for AMI and they could be potential diagnostic tool for AMI.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25184815</pmid><doi>10.1371/journal.pone.0105734</doi><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1559779938 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Adult Aged Anticoagulants Area Under Curve Arteriosclerosis Atherosclerosis Bioindicators Biomarkers Biomarkers - blood Brain Ischemia - blood Brain Ischemia - diagnosis Brain Ischemia - genetics Calcium-binding protein Cardiomyocytes Cardiovascular disease Cardiovascular diseases Case-Control Studies Cell adhesion & migration Cell Hypoxia Cerebral infarction Diagnostic software Diagnostic systems Electrocardiography Embolism Embolisms Enzyme-linked immunosorbent assay Female Gene expression Heart attacks Heart diseases Hospitals Humans Hypertension Hypoxia Infarction Internal medicine Ischemia Kinases Male Medicine Medicine and Health Sciences MicroRNAs MicroRNAs - blood MicroRNAs - genetics Middle Aged miRNA Mortality Myocardial infarction Myocardial Infarction - blood Myocardial Infarction - diagnosis Myocardial Infarction - genetics Myocytes, Cardiac - metabolism Myocytes, Cardiac - pathology Patients Plasma Plasma levels Prostate cancer Pulmonary Embolism - blood Pulmonary Embolism - diagnosis Pulmonary Embolism - genetics ROC Curve Science Stroke Stroke - blood Stroke - diagnosis Stroke - genetics Therapeutic applications Troponin Troponin I Troponin I - blood Troponin I - genetics |
| title | Atherosclerosis-related circulating miRNAs as novel and sensitive predictors for acute myocardial infarction |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T07%3A05%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Atherosclerosis-related%20circulating%20miRNAs%20as%20novel%20and%20sensitive%20predictors%20for%20acute%20myocardial%20infarction&rft.jtitle=PloS%20one&rft.au=Wang,%20Feng&rft.date=2014-09-03&rft.volume=9&rft.issue=9&rft.spage=e105734&rft.pages=e105734-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0105734&rft_dat=%3Cproquest_plos_%3E3421634651%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1559779938&rft_id=info:pmid/25184815&rft_doaj_id=oai_doaj_org_article_49526c518756438c851b31c7f12bd8b5&rfr_iscdi=true |