Preliminary validation of direct detection of foot-and-mouth disease virus within clinical samples using reverse transcription loop-mediated isothermal amplification coupled with a simple lateral flow device for detection

Rapid, field-based diagnostic assays are desirable tools for the control of foot-and-mouth disease (FMD). Current approaches involve either; 1) Detection of FMD virus (FMDV) with immuochromatographic antigen lateral flow devices (LFD), which have relatively low analytical sensitivity, or 2) portable...

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Veröffentlicht in:	PloS one 2014-08, Vol.9 (8), p.e105630-e105630
Hauptverfasser:	Waters, Ryan A, Fowler, Veronica L, Armson, Bryony, Nelson, Noel, Gloster, John, Paton, David J, King, Donald P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens Assaying Biology and Life Sciences Cattle Diagnostic software Diagnostic systems Disease control Electronic components Foot & mouth disease Foot and mouth disease Foot-and-Mouth Disease - diagnosis Foot-and-Mouth Disease - virology Foot-and-Mouth Disease Virus - isolation & purification Infections Laboratories Livestock Mathematical analysis Molecular Diagnostic Techniques - instrumentation Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - instrumentation Nucleic Acid Amplification Techniques - methods Portable equipment Real-Time Polymerase Chain Reaction - instrumentation Real-Time Polymerase Chain Reaction - methods Research and Analysis Methods Reverse transcription Ribonucleic acid RNA Sensitivity Sensitivity analysis Sensitivity and Specificity Serotypes Sheep Swine Transcription (Genetics) Veterinary medicine Viruses
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