A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema
A suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. In this study, we explore whether zebrafish could be a model organism for carrageenan-induced...
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| creator | Huang, Shi-Ying Feng, Chien-Wei Hung, Han-Chun Chakraborty, Chiranjib Chen, Chun-Hong Chen, Wu-Fu Jean, Yen-Hsuan Wang, Hui-Min David Sung, Chun-Sung Sun, Yu-Min Wu, Chang-Yi Liu, Wangta Hsiao, Chung-Der Wen, Zhi-Hong |
| description | A suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. In this study, we explore whether zebrafish could be a model organism for carrageenan-induced abdominal edema. The research results showed that intraperitoneal (i.p.) administration of 1.5% λ-carrageenan in a volume of 20 µL significantly increased abdominal edema in adult zebrafish. Levels of the proinflammatory proteins tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) were increased in carrageenan-injected adult zebrafish during the development of abdominal edema. An associated enhancement was also observed in the leukocyte marker, myeloperoxidase (MPO). To support these results, we further observed that i.p. methylprednisolone (MP; 1 µg), a positive control, significantly inhibited carrageenan-induced inflammation 24 h after carrageenan administration. Furthermore, i.p. pretreatment with either an anti-TNF-α antibody (1∶5 dilution in a volume of 20 µL) or the iNOS-selective inhibitor aminoguanidine (AG; 1 µg) inhibited carrageenan-induced abdominal edema in adult zebrafish. This new animal model is uncomplicated, easy to develop, and involves a straightforward inducement of inflammatory edema for the evaluation of small volumes of drugs or test compounds. |
| doi_str_mv | 10.1371/journal.pone.0104414 |
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In this study, we explore whether zebrafish could be a model organism for carrageenan-induced abdominal edema. The research results showed that intraperitoneal (i.p.) administration of 1.5% λ-carrageenan in a volume of 20 µL significantly increased abdominal edema in adult zebrafish. Levels of the proinflammatory proteins tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) were increased in carrageenan-injected adult zebrafish during the development of abdominal edema. An associated enhancement was also observed in the leukocyte marker, myeloperoxidase (MPO). To support these results, we further observed that i.p. methylprednisolone (MP; 1 µg), a positive control, significantly inhibited carrageenan-induced inflammation 24 h after carrageenan administration. Furthermore, i.p. pretreatment with either an anti-TNF-α antibody (1∶5 dilution in a volume of 20 µL) or the iNOS-selective inhibitor aminoguanidine (AG; 1 µg) inhibited carrageenan-induced abdominal edema in adult zebrafish. This new animal model is uncomplicated, easy to develop, and involves a straightforward inducement of inflammatory edema for the evaluation of small volumes of drugs or test compounds.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0104414</identifier><identifier>PMID: 25141004</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abdomen ; Animals ; Anti-Inflammatory Agents - therapeutic use ; Biology and Life Sciences ; Carrageenan ; Cyprinus carpio ; Danio rerio ; Dilution ; Disease Models, Animal ; Drug development ; Drugs ; Edema ; Edema - chemically induced ; Edema - drug therapy ; Edema - metabolism ; Evaluation ; Inflammation ; Inflammation - chemically induced ; Inflammation - drug therapy ; Inflammation - metabolism ; Leukocytes ; Male ; Medicine and Health Sciences ; Methylprednisolone ; Methylprednisolone - therapeutic use ; Natural products ; Nitric oxide ; Nitric Oxide Synthase Type II - metabolism ; Nitric-oxide synthase ; Peroxidase ; Peroxidase - metabolism ; Pleuronectes platessa ; Proteins ; Research and Analysis Methods ; Rodents ; Studies ; Tumor Necrosis Factor-alpha - metabolism ; Tumor necrosis factor-α ; Zebrafish</subject><ispartof>PloS one, 2014-08, Vol.9 (8), p.e104414</ispartof><rights>2014 Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Huang et al 2014 Huang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-d66c90b963cf64de2941717e4ae84896cde085dbcff7d3f0a46dfe7e5d5c6ac93</citedby><cites>FETCH-LOGICAL-c526t-d66c90b963cf64de2941717e4ae84896cde085dbcff7d3f0a46dfe7e5d5c6ac93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139260/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139260/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,2106,2932,23875,27933,27934,53800,53802</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25141004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gong, Zhiyuan</contributor><creatorcontrib>Huang, Shi-Ying</creatorcontrib><creatorcontrib>Feng, Chien-Wei</creatorcontrib><creatorcontrib>Hung, Han-Chun</creatorcontrib><creatorcontrib>Chakraborty, Chiranjib</creatorcontrib><creatorcontrib>Chen, Chun-Hong</creatorcontrib><creatorcontrib>Chen, Wu-Fu</creatorcontrib><creatorcontrib>Jean, Yen-Hsuan</creatorcontrib><creatorcontrib>Wang, Hui-Min David</creatorcontrib><creatorcontrib>Sung, Chun-Sung</creatorcontrib><creatorcontrib>Sun, Yu-Min</creatorcontrib><creatorcontrib>Wu, Chang-Yi</creatorcontrib><creatorcontrib>Liu, Wangta</creatorcontrib><creatorcontrib>Hsiao, Chung-Der</creatorcontrib><creatorcontrib>Wen, Zhi-Hong</creatorcontrib><title>A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A suitable small animal model may help in the screening and evaluation of new drugs, especially those from natural products, which can be administered at lower dosages, fulfilling an urgent worldwide need. 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In this study, we explore whether zebrafish could be a model organism for carrageenan-induced abdominal edema. The research results showed that intraperitoneal (i.p.) administration of 1.5% λ-carrageenan in a volume of 20 µL significantly increased abdominal edema in adult zebrafish. Levels of the proinflammatory proteins tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) were increased in carrageenan-injected adult zebrafish during the development of abdominal edema. An associated enhancement was also observed in the leukocyte marker, myeloperoxidase (MPO). To support these results, we further observed that i.p. methylprednisolone (MP; 1 µg), a positive control, significantly inhibited carrageenan-induced inflammation 24 h after carrageenan administration. Furthermore, i.p. pretreatment with either an anti-TNF-α antibody (1∶5 dilution in a volume of 20 µL) or the iNOS-selective inhibitor aminoguanidine (AG; 1 µg) inhibited carrageenan-induced abdominal edema in adult zebrafish. This new animal model is uncomplicated, easy to develop, and involves a straightforward inducement of inflammatory edema for the evaluation of small volumes of drugs or test compounds.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25141004</pmid><doi>10.1371/journal.pone.0104414</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2014-08, Vol.9 (8), p.e104414 |
| issn | 1932-6203 1932-6203 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abdomen Animals Anti-Inflammatory Agents - therapeutic use Biology and Life Sciences Carrageenan Cyprinus carpio Danio rerio Dilution Disease Models, Animal Drug development Drugs Edema Edema - chemically induced Edema - drug therapy Edema - metabolism Evaluation Inflammation Inflammation - chemically induced Inflammation - drug therapy Inflammation - metabolism Leukocytes Male Medicine and Health Sciences Methylprednisolone Methylprednisolone - therapeutic use Natural products Nitric oxide Nitric Oxide Synthase Type II - metabolism Nitric-oxide synthase Peroxidase Peroxidase - metabolism Pleuronectes platessa Proteins Research and Analysis Methods Rodents Studies Tumor Necrosis Factor-alpha - metabolism Tumor necrosis factor-α Zebrafish |
| title | A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-03T13%3A40%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20zebrafish%20model%20to%20provide%20mechanistic%20insights%20into%20the%20inflammatory%20events%20in%20carrageenan-induced%20abdominal%20edema&rft.jtitle=PloS%20one&rft.au=Huang,%20Shi-Ying&rft.date=2014-08-20&rft.volume=9&rft.issue=8&rft.spage=e104414&rft.pages=e104414-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0104414&rft_dat=%3Cproquest_plos_%3E3408988971%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1554982628&rft_id=info:pmid/25141004&rft_doaj_id=oai_doaj_org_article_5114e674de8d4106a6f85a85f907faee&rfr_iscdi=true |