Generation and behavior characterization of CaMKIIβ knockout mice
The calcium/calmodulin-dependent protein kinase II (CaMKII) is abundant in the brain, where it makes important contributions to synaptic organization and homeostasis, including playing an essential role in synaptic plasticity and memory. Four genes encode isoforms of CaMKII (α, β, δ, γ), with CaMKII...
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| creator | Bachstetter, Adam D Webster, Scott J Tu, Tao Goulding, Danielle S Haiech, Jacques Watterson, D Martin Van Eldik, Linda J |
| description | The calcium/calmodulin-dependent protein kinase II (CaMKII) is abundant in the brain, where it makes important contributions to synaptic organization and homeostasis, including playing an essential role in synaptic plasticity and memory. Four genes encode isoforms of CaMKII (α, β, δ, γ), with CaMKIIα and CaMKIIβ highly expressed in the brain. Decades of molecular and cellular research, as well as the use of a large number of CaMKIIα mutant mouse lines, have provided insight into the pivotal roles of CaMKIIα in brain plasticity and cognition. However, less is known about the CaMKIIβ isoform. We report the development and extensive behavioral and phenotypic characterization of a CaMKIIβ knockout (KO) mouse. The CaMKIIβ KO mouse was found to be smaller at weaning, with an altered body mass composition. The CaMKIIβ KO mouse showed ataxia, impaired forelimb grip strength, and deficits in the rotorod, balance beam and running wheel tasks. Interestingly, the CaMKIIβ KO mouse exhibited reduced anxiety in the elevated plus maze and open field tests. The CaMKIIβ KO mouse also showed cognitive impairment in the novel object recognition task. Our results provide a comprehensive behavioral characterization of mice deficient in the β isoform of CaMKII. The neurologic phenotypes and the construction of the genotype suggest the utility of this KO mouse strain for future studies of CaMKIIβ in brain structure, function and development. |
| doi_str_mv | 10.1371/journal.pone.0105191 |
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C.</contributor><creatorcontrib>Bachstetter, Adam D ; Webster, Scott J ; Tu, Tao ; Goulding, Danielle S ; Haiech, Jacques ; Watterson, D Martin ; Van Eldik, Linda J ; Skoulakis, Efthimios M. C.</creatorcontrib><description>The calcium/calmodulin-dependent protein kinase II (CaMKII) is abundant in the brain, where it makes important contributions to synaptic organization and homeostasis, including playing an essential role in synaptic plasticity and memory. Four genes encode isoforms of CaMKII (α, β, δ, γ), with CaMKIIα and CaMKIIβ highly expressed in the brain. Decades of molecular and cellular research, as well as the use of a large number of CaMKIIα mutant mouse lines, have provided insight into the pivotal roles of CaMKIIα in brain plasticity and cognition. However, less is known about the CaMKIIβ isoform. We report the development and extensive behavioral and phenotypic characterization of a CaMKIIβ knockout (KO) mouse. The CaMKIIβ KO mouse was found to be smaller at weaning, with an altered body mass composition. The CaMKIIβ KO mouse showed ataxia, impaired forelimb grip strength, and deficits in the rotorod, balance beam and running wheel tasks. Interestingly, the CaMKIIβ KO mouse exhibited reduced anxiety in the elevated plus maze and open field tests. The CaMKIIβ KO mouse also showed cognitive impairment in the novel object recognition task. Our results provide a comprehensive behavioral characterization of mice deficient in the β isoform of CaMKII. The neurologic phenotypes and the construction of the genotype suggest the utility of this KO mouse strain for future studies of CaMKIIβ in brain structure, function and development.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0105191</identifier><identifier>PMID: 25127391</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aging ; Alzheimer's disease ; Alzheimers disease ; Animals ; Anxiety ; Anxiety - enzymology ; Ataxia ; Ataxia - enzymology ; Behavior ; Behavioral plasticity ; Biology and Life Sciences ; Body Composition ; Body mass ; Brain ; Ca2+/calmodulin-dependent protein kinase II ; Calcium ; Calcium-binding protein ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism ; Calmodulin ; Cloning ; Cognition ; Cognition Disorders - enzymology ; Cognitive ability ; Field tests ; Functional anatomy ; Genotypes ; Grip strength ; Homeostasis ; Isoforms ; Kinases ; Laboratory animals ; Male ; Memory ; Mice, Inbred C57BL ; Mice, Knockout ; Muscle Strength ; Nesting Behavior ; Neurosciences ; Object recognition ; Pattern recognition ; Pattern Recognition, Physiological ; Plasticity ; Proteins ; Research and Analysis Methods ; Rodents ; Rotarod Performance Test ; Structure-function relationships ; Synaptic plasticity ; Transgenic animals ; Weaning ; Weight Gain ; Wheel running</subject><ispartof>PloS one, 2014-08, Vol.9 (8), p.e105191-e105191</ispartof><rights>2014 Bachstetter et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Bachstetter et al 2014 Bachstetter et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-a05e1b4a328fd58ac55badecacc0a792c0b89374b6206d5ef3b020fe82ec72013</citedby><cites>FETCH-LOGICAL-c526t-a05e1b4a328fd58ac55badecacc0a792c0b89374b6206d5ef3b020fe82ec72013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134274/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134274/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25127391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Skoulakis, Efthimios M. C.</contributor><creatorcontrib>Bachstetter, Adam D</creatorcontrib><creatorcontrib>Webster, Scott J</creatorcontrib><creatorcontrib>Tu, Tao</creatorcontrib><creatorcontrib>Goulding, Danielle S</creatorcontrib><creatorcontrib>Haiech, Jacques</creatorcontrib><creatorcontrib>Watterson, D Martin</creatorcontrib><creatorcontrib>Van Eldik, Linda J</creatorcontrib><title>Generation and behavior characterization of CaMKIIβ knockout mice</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The calcium/calmodulin-dependent protein kinase II (CaMKII) is abundant in the brain, where it makes important contributions to synaptic organization and homeostasis, including playing an essential role in synaptic plasticity and memory. Four genes encode isoforms of CaMKII (α, β, δ, γ), with CaMKIIα and CaMKIIβ highly expressed in the brain. Decades of molecular and cellular research, as well as the use of a large number of CaMKIIα mutant mouse lines, have provided insight into the pivotal roles of CaMKIIα in brain plasticity and cognition. However, less is known about the CaMKIIβ isoform. We report the development and extensive behavioral and phenotypic characterization of a CaMKIIβ knockout (KO) mouse. The CaMKIIβ KO mouse was found to be smaller at weaning, with an altered body mass composition. The CaMKIIβ KO mouse showed ataxia, impaired forelimb grip strength, and deficits in the rotorod, balance beam and running wheel tasks. Interestingly, the CaMKIIβ KO mouse exhibited reduced anxiety in the elevated plus maze and open field tests. The CaMKIIβ KO mouse also showed cognitive impairment in the novel object recognition task. Our results provide a comprehensive behavioral characterization of mice deficient in the β isoform of CaMKII. The neurologic phenotypes and the construction of the genotype suggest the utility of this KO mouse strain for future studies of CaMKIIβ in brain structure, function and development.</description><subject>Aging</subject><subject>Alzheimer's disease</subject><subject>Alzheimers disease</subject><subject>Animals</subject><subject>Anxiety</subject><subject>Anxiety - enzymology</subject><subject>Ataxia</subject><subject>Ataxia - enzymology</subject><subject>Behavior</subject><subject>Behavioral plasticity</subject><subject>Biology and Life Sciences</subject><subject>Body Composition</subject><subject>Body mass</subject><subject>Brain</subject><subject>Ca2+/calmodulin-dependent protein kinase II</subject><subject>Calcium</subject><subject>Calcium-binding protein</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism</subject><subject>Calmodulin</subject><subject>Cloning</subject><subject>Cognition</subject><subject>Cognition Disorders - enzymology</subject><subject>Cognitive ability</subject><subject>Field tests</subject><subject>Functional anatomy</subject><subject>Genotypes</subject><subject>Grip strength</subject><subject>Homeostasis</subject><subject>Isoforms</subject><subject>Kinases</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Memory</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Muscle Strength</subject><subject>Nesting Behavior</subject><subject>Neurosciences</subject><subject>Object recognition</subject><subject>Pattern recognition</subject><subject>Pattern Recognition, Physiological</subject><subject>Plasticity</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Rodents</subject><subject>Rotarod Performance Test</subject><subject>Structure-function relationships</subject><subject>Synaptic plasticity</subject><subject>Transgenic animals</subject><subject>Weaning</subject><subject>Weight Gain</subject><subject>Wheel running</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1v1DAUtBAVLYV_gCBSL73s4s_EviDBqrQrirjA2Xp2nrvZZu3FSSqVn8UP4TeR7aZVizg9672Z8Yw0hLxhdM5Exd6v05AjtPNtijinjCpm2DNyxIzgs5JT8fzR-5C87Lo1pUrosnxBDrlivBKGHZFP5xgxQ9-kWECsC4cruGlSLvwKMvgec_Nrf02hWMDXL8vln9_FdUz-Og19sWk8viIHAdoOX0_zmPz4fPZ9cTG7_Ha-XHy8nHnFy34GVCFzEgTXoVYavFIOavTgPYXKcE-dNqKSbjRc1gqDcJTTgJqjrzhl4pi82-tu29TZKX5nmVJCCW5YOSKWe0SdYG23udlAvrUJGnu3SPnKQu4b36KVVRmc9sYppWVQqIOotdcOudaahZ3Wh-m3wW2w9hj7DO0T0aeX2KzsVbqxkgnJKzkKnE4COf0csOvtpuk8ti1ETMOdbykrIY0ZoSf_QP-fTu5RPqeuyxgezDBqd5W4Z9ldJexUiZH29nGQB9J9B8Rf0uO1Ww</recordid><startdate>20140815</startdate><enddate>20140815</enddate><creator>Bachstetter, Adam D</creator><creator>Webster, Scott J</creator><creator>Tu, Tao</creator><creator>Goulding, Danielle S</creator><creator>Haiech, Jacques</creator><creator>Watterson, D Martin</creator><creator>Van Eldik, Linda J</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140815</creationdate><title>Generation and behavior characterization of CaMKIIβ knockout mice</title><author>Bachstetter, Adam D ; Webster, Scott J ; Tu, Tao ; Goulding, Danielle S ; Haiech, Jacques ; Watterson, D Martin ; Van Eldik, Linda J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-a05e1b4a328fd58ac55badecacc0a792c0b89374b6206d5ef3b020fe82ec72013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aging</topic><topic>Alzheimer's disease</topic><topic>Alzheimers disease</topic><topic>Animals</topic><topic>Anxiety</topic><topic>Anxiety - enzymology</topic><topic>Ataxia</topic><topic>Ataxia - enzymology</topic><topic>Behavior</topic><topic>Behavioral plasticity</topic><topic>Biology and Life Sciences</topic><topic>Body Composition</topic><topic>Body mass</topic><topic>Brain</topic><topic>Ca2+/calmodulin-dependent protein kinase II</topic><topic>Calcium</topic><topic>Calcium-binding protein</topic><topic>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics</topic><topic>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism</topic><topic>Calmodulin</topic><topic>Cloning</topic><topic>Cognition</topic><topic>Cognition Disorders - enzymology</topic><topic>Cognitive ability</topic><topic>Field tests</topic><topic>Functional anatomy</topic><topic>Genotypes</topic><topic>Grip strength</topic><topic>Homeostasis</topic><topic>Isoforms</topic><topic>Kinases</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Memory</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Muscle Strength</topic><topic>Nesting Behavior</topic><topic>Neurosciences</topic><topic>Object recognition</topic><topic>Pattern recognition</topic><topic>Pattern Recognition, Physiological</topic><topic>Plasticity</topic><topic>Proteins</topic><topic>Research and Analysis Methods</topic><topic>Rodents</topic><topic>Rotarod Performance Test</topic><topic>Structure-function relationships</topic><topic>Synaptic plasticity</topic><topic>Transgenic animals</topic><topic>Weaning</topic><topic>Weight Gain</topic><topic>Wheel running</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bachstetter, Adam D</creatorcontrib><creatorcontrib>Webster, Scott J</creatorcontrib><creatorcontrib>Tu, Tao</creatorcontrib><creatorcontrib>Goulding, Danielle S</creatorcontrib><creatorcontrib>Haiech, Jacques</creatorcontrib><creatorcontrib>Watterson, D Martin</creatorcontrib><creatorcontrib>Van Eldik, Linda J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation and behavior characterization of CaMKIIβ knockout mice</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-08-15</date><risdate>2014</risdate><volume>9</volume><issue>8</issue><spage>e105191</spage><epage>e105191</epage><pages>e105191-e105191</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The calcium/calmodulin-dependent protein kinase II (CaMKII) is abundant in the brain, where it makes important contributions to synaptic organization and homeostasis, including playing an essential role in synaptic plasticity and memory. Four genes encode isoforms of CaMKII (α, β, δ, γ), with CaMKIIα and CaMKIIβ highly expressed in the brain. Decades of molecular and cellular research, as well as the use of a large number of CaMKIIα mutant mouse lines, have provided insight into the pivotal roles of CaMKIIα in brain plasticity and cognition. However, less is known about the CaMKIIβ isoform. We report the development and extensive behavioral and phenotypic characterization of a CaMKIIβ knockout (KO) mouse. The CaMKIIβ KO mouse was found to be smaller at weaning, with an altered body mass composition. The CaMKIIβ KO mouse showed ataxia, impaired forelimb grip strength, and deficits in the rotorod, balance beam and running wheel tasks. Interestingly, the CaMKIIβ KO mouse exhibited reduced anxiety in the elevated plus maze and open field tests. The CaMKIIβ KO mouse also showed cognitive impairment in the novel object recognition task. Our results provide a comprehensive behavioral characterization of mice deficient in the β isoform of CaMKII. The neurologic phenotypes and the construction of the genotype suggest the utility of this KO mouse strain for future studies of CaMKIIβ in brain structure, function and development.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25127391</pmid><doi>10.1371/journal.pone.0105191</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Aging Alzheimer's disease Alzheimers disease Animals Anxiety Anxiety - enzymology Ataxia Ataxia - enzymology Behavior Behavioral plasticity Biology and Life Sciences Body Composition Body mass Brain Ca2+/calmodulin-dependent protein kinase II Calcium Calcium-binding protein Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism Calmodulin Cloning Cognition Cognition Disorders - enzymology Cognitive ability Field tests Functional anatomy Genotypes Grip strength Homeostasis Isoforms Kinases Laboratory animals Male Memory Mice, Inbred C57BL Mice, Knockout Muscle Strength Nesting Behavior Neurosciences Object recognition Pattern recognition Pattern Recognition, Physiological Plasticity Proteins Research and Analysis Methods Rodents Rotarod Performance Test Structure-function relationships Synaptic plasticity Transgenic animals Weaning Weight Gain Wheel running |
| title | Generation and behavior characterization of CaMKIIβ knockout mice |
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