SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins
The aim of this study was to explore the role of SecDF in protein secretion in Bacillus cereus ATCC 14579 by in-depth characterization of a markerless secDF knock out mutant. Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology,...
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| Veröffentlicht in: | PloS one 2014-08, Vol.9 (8), p.e103326 |
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| creator | Vörös, Aniko Simm, Roger Slamti, Leyla McKay, Matthew J Hegna, Ida K Nielsen-LeRoux, Christina Hassan, Karl A Paulsen, Ian T Lereclus, Didier Økstad, Ole Andreas Molloy, Mark P Kolstø, Anne-Brit |
| description | The aim of this study was to explore the role of SecDF in protein secretion in Bacillus cereus ATCC 14579 by in-depth characterization of a markerless secDF knock out mutant. Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. This study shows that SecDF is an important part of the Sec-translocase mediating efficient secretion of virulence factors in the Gram-positive opportunistic pathogen B. cereus, and further supports the notion that B. cereus enterotoxins are secreted by the Sec-system. |
| doi_str_mv | 10.1371/journal.pone.0103326 |
| format | Article |
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Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. This study shows that SecDF is an important part of the Sec-translocase mediating efficient secretion of virulence factors in the Gram-positive opportunistic pathogen B. cereus, and further supports the notion that B. cereus enterotoxins are secreted by the Sec-system.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0103326</identifier><identifier>PMID: 25083861</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Bacillus cereus ; Bacillus cereus - drug effects ; Bacillus cereus - physiology ; Bacillus cereus - ultrastructure ; Bacillus subtilis ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Toxins - biosynthesis ; Biological Transport ; Biology and Life Sciences ; Cell division ; Cell morphology ; Cell Wall - metabolism ; Cell walls ; Clonal deletion ; Cloning ; Cytology ; Drug resistance ; Drug Resistance, Bacterial - genetics ; E coli ; Enterotoxins ; Escherichia coli ; Experiments ; Food contamination ; Food poisoning ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Gene Knockout Techniques ; Glucose ; Growth rate ; Homeostasis ; Laboratories ; Life Sciences ; Mass spectrometry ; Mass spectroscopy ; Medicine and Health Sciences ; Moths - microbiology ; Mutation ; Opportunist infection ; Pharmaceuticals ; Pharmacy ; Phospholipase ; Phospholipase C ; Proteins ; Proteomics ; Secretion ; Secretome ; Staphylococcus aureus ; Stress, Physiological ; Temperature ; Toxins ; Transcription ; Translocase ; Virulence ; Virulence (Microbiology) ; Virulence - genetics ; Virulence factors ; Xenobiotics ; Xenobiotics - pharmacology</subject><ispartof>PloS one, 2014-08, Vol.9 (8), p.e103326</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Vörös et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. 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genetics</subject><subject>E coli</subject><subject>Enterotoxins</subject><subject>Escherichia coli</subject><subject>Experiments</subject><subject>Food contamination</subject><subject>Food poisoning</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Knockout Techniques</subject><subject>Glucose</subject><subject>Growth rate</subject><subject>Homeostasis</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicine and Health Sciences</subject><subject>Moths - microbiology</subject><subject>Mutation</subject><subject>Opportunist infection</subject><subject>Pharmaceuticals</subject><subject>Pharmacy</subject><subject>Phospholipase</subject><subject>Phospholipase C</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Secretion</subject><subject>Secretome</subject><subject>Staphylococcus aureus</subject><subject>Stress, Physiological</subject><subject>Temperature</subject><subject>Toxins</subject><subject>Transcription</subject><subject>Translocase</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><subject>Virulence - 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Deletion of secDF resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. Most toxins, including food poisoning-associated enterotoxins Nhe, Hbl, and cytotoxin K, as well as phospholipase C were less abundant in the secretome of the ΔsecDF mutant as determined by label-free mass spectrometry. Global transcriptome studies revealed profound transcriptional changes upon deletion of secDF indicating cell envelope stress. Interestingly, the addition of glucose enhanced the described phenotypes. This study shows that SecDF is an important part of the Sec-translocase mediating efficient secretion of virulence factors in the Gram-positive opportunistic pathogen B. cereus, and further supports the notion that B. cereus enterotoxins are secreted by the Sec-system.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25083861</pmid><doi>10.1371/journal.pone.0103326</doi><orcidid>https://orcid.org/0000-0003-0275-8336</orcidid><orcidid>https://orcid.org/0000-0001-9047-9104</orcidid><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1550514239 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Bacillus cereus Bacillus cereus - drug effects Bacillus cereus - physiology Bacillus cereus - ultrastructure Bacillus subtilis Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins - biosynthesis Biological Transport Biology and Life Sciences Cell division Cell morphology Cell Wall - metabolism Cell walls Clonal deletion Cloning Cytology Drug resistance Drug Resistance, Bacterial - genetics E coli Enterotoxins Escherichia coli Experiments Food contamination Food poisoning Gene expression Gene Expression Profiling Gene Expression Regulation, Bacterial Gene Knockout Techniques Glucose Growth rate Homeostasis Laboratories Life Sciences Mass spectrometry Mass spectroscopy Medicine and Health Sciences Moths - microbiology Mutation Opportunist infection Pharmaceuticals Pharmacy Phospholipase Phospholipase C Proteins Proteomics Secretion Secretome Staphylococcus aureus Stress, Physiological Temperature Toxins Transcription Translocase Virulence Virulence (Microbiology) Virulence - genetics Virulence factors Xenobiotics Xenobiotics - pharmacology |
| title | SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T17%3A18%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=SecDF%20as%20part%20of%20the%20Sec-translocase%20facilitates%20efficient%20secretion%20of%20Bacillus%20cereus%20toxins%20and%20cell%20wall-associated%20proteins&rft.jtitle=PloS%20one&rft.au=V%C3%B6r%C3%B6s,%20Aniko&rft.date=2014-08-01&rft.volume=9&rft.issue=8&rft.spage=e103326&rft.pages=e103326-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0103326&rft_dat=%3Cgale_plos_%3EA416683547%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1550514239&rft_id=info:pmid/25083861&rft_galeid=A416683547&rft_doaj_id=oai_doaj_org_article_fe3badada9cc40dca2083f4b3520283b&rfr_iscdi=true |