Establishment and clinical applications of a portable system for capturing influenza viruses released through coughing
Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equ...
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| creator | Hatagishi, Etsuko Okamoto, Michiko Ohmiya, Suguru Yano, Hisakazu Hori, Toru Saito, Wakana Miki, Hiroshi Suzuki, Yasushi Saito, Reiko Yamamoto, Taro Shoji, Makoto Morisaki, Yoshihisa Sakata, Soichiro Nishimura, Hidekazu |
| description | Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equipped with an air sampler to draw air and pass it through a gelatin membrane filter connected to a cone-shaped, megaphone-like device to guide the cough airflow to the membrane. The membrane was dissolved in a medium, and the viral load was measured using quantitative real-time reverse transcriptase-polymerase chain reaction and a plaque assay. The approximate viral recovery rate of this system was 10% in simulation experiments to collect and quantify the viral particles aerosolized by a nebulizer. Using this system, cough samples were collected from 56 influenza A patients. The total viral detection rate was 41% (23/56), and the viral loads varied significantly (from |
| doi_str_mv | 10.1371/journal.pone.0103560 |
| format | Article |
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We developed a portable airborne particle-collection system to measure the viral load; it is equipped with an air sampler to draw air and pass it through a gelatin membrane filter connected to a cone-shaped, megaphone-like device to guide the cough airflow to the membrane. The membrane was dissolved in a medium, and the viral load was measured using quantitative real-time reverse transcriptase-polymerase chain reaction and a plaque assay. The approximate viral recovery rate of this system was 10% in simulation experiments to collect and quantify the viral particles aerosolized by a nebulizer. Using this system, cough samples were collected from 56 influenza A patients. The total viral detection rate was 41% (23/56), and the viral loads varied significantly (from <10, less than the detection limit, to 2240 viral gene copies/cough). Viable viruses were detected from 3 samples with ≤18 plaque forming units per cough sample. The virus detection rates were similar among different groups of patients infected with different viral subtypes and during different influenza seasons. Among patients who did not receive antiviral treatment, viruses were detected in one of six cases in the vaccinated group and four of six cases in the unvaccinated group. We found cases with high viral titers in throat swabs or oral secretions but very low or undetectable in coughs and vice versa suggesting other possible anatomical sites where the viruses might be mixed into the cough. Our system is easy to operate, appropriate for bedside use, and is useful for comparing the viral load in cough samples from influenza patients under various conditions and settings. However, further large-scale studies are warranted to validate our results.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0103560</identifier><identifier>PMID: 25083787</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aerosols ; Air flow ; Airborne sensing ; Airflow ; Animals ; Antiviral agents ; Biology and Life Sciences ; Cell Line ; Collection ; Cough ; Cough - virology ; Disease control ; Disease transmission ; DNA polymerases ; Dogs ; Female ; Gelatin ; Hospitals ; Humans ; Infections ; Infectious diseases ; Influenza ; Influenza A ; Influenza viruses ; Influenza, Human - transmission ; Male ; Medical research ; Medicine ; Medicine and Health Sciences ; Membrane filters ; Middle Aged ; Orthomyxoviridae - genetics ; Orthomyxoviridae - isolation & purification ; Patients ; Pharynx ; Plaque assay ; Polymerase chain reaction ; Public health ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; RNA-directed DNA polymerase ; Secretions ; Studies ; Therapeutic applications ; Vaccination ; Viral Load ; Viruses ; Young Adult</subject><ispartof>PloS one, 2014-08, Vol.9 (8), p.e103560-e103560</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Hatagishi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Hatagishi et al 2014 Hatagishi et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c831t-c4e16b37893977d972264c2b8b29eabb0a7ed3e92fbd1322d72f33027a283d383</citedby><cites>FETCH-LOGICAL-c831t-c4e16b37893977d972264c2b8b29eabb0a7ed3e92fbd1322d72f33027a283d383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118893/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4118893/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25083787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Yen, Hui-Ling</contributor><creatorcontrib>Hatagishi, Etsuko</creatorcontrib><creatorcontrib>Okamoto, Michiko</creatorcontrib><creatorcontrib>Ohmiya, Suguru</creatorcontrib><creatorcontrib>Yano, Hisakazu</creatorcontrib><creatorcontrib>Hori, Toru</creatorcontrib><creatorcontrib>Saito, Wakana</creatorcontrib><creatorcontrib>Miki, Hiroshi</creatorcontrib><creatorcontrib>Suzuki, Yasushi</creatorcontrib><creatorcontrib>Saito, Reiko</creatorcontrib><creatorcontrib>Yamamoto, Taro</creatorcontrib><creatorcontrib>Shoji, Makoto</creatorcontrib><creatorcontrib>Morisaki, Yoshihisa</creatorcontrib><creatorcontrib>Sakata, Soichiro</creatorcontrib><creatorcontrib>Nishimura, Hidekazu</creatorcontrib><title>Establishment and clinical applications of a portable system for capturing influenza viruses released through coughing</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equipped with an air sampler to draw air and pass it through a gelatin membrane filter connected to a cone-shaped, megaphone-like device to guide the cough airflow to the membrane. The membrane was dissolved in a medium, and the viral load was measured using quantitative real-time reverse transcriptase-polymerase chain reaction and a plaque assay. The approximate viral recovery rate of this system was 10% in simulation experiments to collect and quantify the viral particles aerosolized by a nebulizer. Using this system, cough samples were collected from 56 influenza A patients. The total viral detection rate was 41% (23/56), and the viral loads varied significantly (from <10, less than the detection limit, to 2240 viral gene copies/cough). Viable viruses were detected from 3 samples with ≤18 plaque forming units per cough sample. The virus detection rates were similar among different groups of patients infected with different viral subtypes and during different influenza seasons. Among patients who did not receive antiviral treatment, viruses were detected in one of six cases in the vaccinated group and four of six cases in the unvaccinated group. We found cases with high viral titers in throat swabs or oral secretions but very low or undetectable in coughs and vice versa suggesting other possible anatomical sites where the viruses might be mixed into the cough. Our system is easy to operate, appropriate for bedside use, and is useful for comparing the viral load in cough samples from influenza patients under various conditions and settings. However, further large-scale studies are warranted to validate our results.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aerosols</subject><subject>Air flow</subject><subject>Airborne sensing</subject><subject>Airflow</subject><subject>Animals</subject><subject>Antiviral agents</subject><subject>Biology and Life Sciences</subject><subject>Cell Line</subject><subject>Collection</subject><subject>Cough</subject><subject>Cough - virology</subject><subject>Disease control</subject><subject>Disease transmission</subject><subject>DNA polymerases</subject><subject>Dogs</subject><subject>Female</subject><subject>Gelatin</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza viruses</subject><subject>Influenza, Human - transmission</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Membrane filters</subject><subject>Middle Aged</subject><subject>Orthomyxoviridae - genetics</subject><subject>Orthomyxoviridae - isolation & purification</subject><subject>Patients</subject><subject>Pharynx</subject><subject>Plaque assay</subject><subject>Polymerase chain reaction</subject><subject>Public health</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA-directed DNA polymerase</subject><subject>Secretions</subject><subject>Studies</subject><subject>Therapeutic applications</subject><subject>Vaccination</subject><subject>Viral Load</subject><subject>Viruses</subject><subject>Young 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Toru</au><au>Saito, Wakana</au><au>Miki, Hiroshi</au><au>Suzuki, Yasushi</au><au>Saito, Reiko</au><au>Yamamoto, Taro</au><au>Shoji, Makoto</au><au>Morisaki, Yoshihisa</au><au>Sakata, Soichiro</au><au>Nishimura, Hidekazu</au><au>Yen, Hui-Ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment and clinical applications of a portable system for capturing influenza viruses released through coughing</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>9</volume><issue>8</issue><spage>e103560</spage><epage>e103560</epage><pages>e103560-e103560</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Coughing plays an important role in influenza transmission; however, there is insufficient information regarding the viral load in cough because of the lack of convenient and reliable collection methods. We developed a portable airborne particle-collection system to measure the viral load; it is equipped with an air sampler to draw air and pass it through a gelatin membrane filter connected to a cone-shaped, megaphone-like device to guide the cough airflow to the membrane. The membrane was dissolved in a medium, and the viral load was measured using quantitative real-time reverse transcriptase-polymerase chain reaction and a plaque assay. The approximate viral recovery rate of this system was 10% in simulation experiments to collect and quantify the viral particles aerosolized by a nebulizer. Using this system, cough samples were collected from 56 influenza A patients. The total viral detection rate was 41% (23/56), and the viral loads varied significantly (from <10, less than the detection limit, to 2240 viral gene copies/cough). Viable viruses were detected from 3 samples with ≤18 plaque forming units per cough sample. The virus detection rates were similar among different groups of patients infected with different viral subtypes and during different influenza seasons. Among patients who did not receive antiviral treatment, viruses were detected in one of six cases in the vaccinated group and four of six cases in the unvaccinated group. We found cases with high viral titers in throat swabs or oral secretions but very low or undetectable in coughs and vice versa suggesting other possible anatomical sites where the viruses might be mixed into the cough. Our system is easy to operate, appropriate for bedside use, and is useful for comparing the viral load in cough samples from influenza patients under various conditions and settings. However, further large-scale studies are warranted to validate our results.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25083787</pmid><doi>10.1371/journal.pone.0103560</doi><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_plos_journals_1550514186 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; PMC (PubMed Central); DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Adolescent Adult Aerosols Air flow Airborne sensing Airflow Animals Antiviral agents Biology and Life Sciences Cell Line Collection Cough Cough - virology Disease control Disease transmission DNA polymerases Dogs Female Gelatin Hospitals Humans Infections Infectious diseases Influenza Influenza A Influenza viruses Influenza, Human - transmission Male Medical research Medicine Medicine and Health Sciences Membrane filters Middle Aged Orthomyxoviridae - genetics Orthomyxoviridae - isolation & purification Patients Pharynx Plaque assay Polymerase chain reaction Public health Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA-directed DNA polymerase Secretions Studies Therapeutic applications Vaccination Viral Load Viruses Young Adult |
| title | Establishment and clinical applications of a portable system for capturing influenza viruses released through coughing |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T00%3A29%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Establishment%20and%20clinical%20applications%20of%20a%20portable%20system%20for%20capturing%20influenza%20viruses%20released%20through%20coughing&rft.jtitle=PloS%20one&rft.au=Hatagishi,%20Etsuko&rft.date=2014-08-01&rft.volume=9&rft.issue=8&rft.spage=e103560&rft.epage=e103560&rft.pages=e103560-e103560&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0103560&rft_dat=%3Cgale_plos_%3EA416683367%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1550514186&rft_id=info:pmid/25083787&rft_galeid=A416683367&rft_doaj_id=oai_doaj_org_article_c2d9c11dd3e34040b4e87ffa02846d3b&rfr_iscdi=true |