Visualized gene network reveals the novel target transcripts Sox2 and Pax6 of neuronal development in trans-placental exposure to bisphenol A
Bisphenol A (BPA) is a ubiquitous endocrine disrupting chemical in our daily life, and its health effect in response to prenatal exposure is still controversial. Early-life BPA exposure may impact brain development and contribute to childhood neurological disorders. The aim of the present study was...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-07, Vol.9 (7), p.e100576 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 7 |
| container_start_page | e100576 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Yang, Chung-Wei Chou, Wei-Chun Chen, Kuan-Hsueh Cheng, An-Lin Mao, I-Fang Chao, How-Ran Chuang, Chun-Yu |
| description | Bisphenol A (BPA) is a ubiquitous endocrine disrupting chemical in our daily life, and its health effect in response to prenatal exposure is still controversial. Early-life BPA exposure may impact brain development and contribute to childhood neurological disorders. The aim of the present study was to investigate molecular target genes of neuronal development in trans-placental exposure to BPA.
A meta-analysis of three public microarray datasets was performed to screen for differentially expressed genes (DEGs) in exposure to BPA. The candidate genes of neuronal development were identified from gene ontology analysis in a reconstructed neuronal sub-network, and their gene expressions were determined using real-time PCR in 20 umbilical cord blood samples dichotomized into high and low BPA level groups upon the median 16.8 nM.
Among 36 neuronal transcripts sorted from DAVID ontology clusters of 457 DEGs using the analysis of Bioconductor limma package, we found two neuronal genes, sex determining region Y-box 2 (Sox2) and paired box 6 (Pax6), had preferentially down-regulated expression (Bonferroni correction p-value |
| doi_str_mv | 10.1371/journal.pone.0100576 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1547695967</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A418126780</galeid><doaj_id>oai_doaj_org_article_311ac940b28b41f1988fbe6d32f20635</doaj_id><sourcerecordid>A418126780</sourcerecordid><originalsourceid>FETCH-LOGICAL-c758t-1f010cea681f85b5764ae032dbca30bbcd9b18bc81cfb626874ea7a5457817013</originalsourceid><addsrcrecordid>eNqNk22L1DAQx4so3nn6DUQDguCLXZM-pNk3wnL4sHBw4um9DWk67ebsJjVJ19Xv4Hd21u0dW1CQvkiZ_P7_zAwzSfKU0TnLSvb6xg3eqm7eOwtzyigtSn4vOWWLLJ3xlGb3j_5Pkkch3CCSCc4fJidpQQuGgtPk17UJg-rMT6hJCxaIhfjd-a_EwxZUF0hcY8xtoSNR-RYiiV7ZoL3pYyBXbpcSZWvyUe04cQ2qB-8wK1KjvHP9Bmwkxh5Es75TGgN4DbvehcEDiY5UJvRrsK4jy8fJgwYfhSfjeZZ8eff28_mH2cXl-9X58mKmy0LEGWuwXg2KC9aIosLCcwU0S-tKq4xWla4XFROVFkw3FU-5KHNQpSryohSspCw7S54ffPvOBTl2MkhW5CVfFAteIrE6ELVTN7L3ZqP8D-mUkX8CzrdS-Wh0BzJjTOlFTqtUVDlr2EKIpgJeZ2mTUp4V6PVmfG2oNlDvW-BVNzGd3lizlq3bypxRjgWjwYvRwLtvA4T4j5RHqlWYlbGNQzO9MUHLZc4ES3kpKFLzv1D41bAxGmepMRifCF5NBMhE2MVWDSHI1dWn_2cvr6fsyyN2jcMW18F1QzTOhimYH0DtXQgemrvOMSr3q3DbDblfBTmuAsqeHXf9TnQ7-9lvdCYGEw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1547695967</pqid></control><display><type>article</type><title>Visualized gene network reveals the novel target transcripts Sox2 and Pax6 of neuronal development in trans-placental exposure to bisphenol A</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Yang, Chung-Wei ; Chou, Wei-Chun ; Chen, Kuan-Hsueh ; Cheng, An-Lin ; Mao, I-Fang ; Chao, How-Ran ; Chuang, Chun-Yu</creator><contributor>Chadwick, Brian P.</contributor><creatorcontrib>Yang, Chung-Wei ; Chou, Wei-Chun ; Chen, Kuan-Hsueh ; Cheng, An-Lin ; Mao, I-Fang ; Chao, How-Ran ; Chuang, Chun-Yu ; Chadwick, Brian P.</creatorcontrib><description>Bisphenol A (BPA) is a ubiquitous endocrine disrupting chemical in our daily life, and its health effect in response to prenatal exposure is still controversial. Early-life BPA exposure may impact brain development and contribute to childhood neurological disorders. The aim of the present study was to investigate molecular target genes of neuronal development in trans-placental exposure to BPA.
A meta-analysis of three public microarray datasets was performed to screen for differentially expressed genes (DEGs) in exposure to BPA. The candidate genes of neuronal development were identified from gene ontology analysis in a reconstructed neuronal sub-network, and their gene expressions were determined using real-time PCR in 20 umbilical cord blood samples dichotomized into high and low BPA level groups upon the median 16.8 nM.
Among 36 neuronal transcripts sorted from DAVID ontology clusters of 457 DEGs using the analysis of Bioconductor limma package, we found two neuronal genes, sex determining region Y-box 2 (Sox2) and paired box 6 (Pax6), had preferentially down-regulated expression (Bonferroni correction p-value <10(-4) and log2-transformed fold change ≤-1.2) in response to BPA exposure. Fetal cord blood samples had the obviously attenuated gene expression of Sox2 and Pax6 in high BPA group referred to low BPA group. Visualized gene network of Cytoscape analysis showed that Sox2 and Pax6 which were contributed to neural precursor cell proliferation and neuronal differentiation might be down-regulated through sonic hedgehog (Shh), vascular endothelial growth factor A (VEGFA) and Notch signaling.
These results indicated that trans-placental BPA exposure down-regulated gene expression of Sox2 and Pax6 potentially underlying the adverse effect on childhood neuronal development.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0100576</identifier><identifier>PMID: 25051057</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Alcohol ; Analysis ; Benzhydryl Compounds - toxicity ; Biology and Life Sciences ; Biomedical engineering ; Bisphenol A ; Blood ; Brain ; Cell proliferation ; Central Nervous System - drug effects ; Central Nervous System - embryology ; Children ; Cluster analysis ; Cord blood ; DNA methylation ; DNA microarrays ; Endocrine disruptors ; Endocrine Disruptors - toxicity ; Endothelial growth factors ; Engineering ; Environmental Exposure ; Environmental science ; Epigenetics ; Exposure ; Eye Proteins - genetics ; Eye Proteins - metabolism ; Female ; Fetuses ; Gene expression ; Gene Ontology ; Gene Regulatory Networks ; Genes ; Health aspects ; Hedgehog protein ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Hypothesis testing ; Insulin ; Insulin-like growth factors ; Kinases ; Maternal-Fetal Exchange ; Medicine and Health Sciences ; Nervous system diseases ; Neurological diseases ; Neurons ; Notch protein ; Paired Box Transcription Factors - genetics ; Paired Box Transcription Factors - metabolism ; Pax6 protein ; PAX6 Transcription Factor ; Phenols ; Phenols (Class of compounds) ; Phenols - toxicity ; Placenta ; Pregnancy ; Pregnant women ; Prenatal experience ; Prenatal exposure ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Rodents ; Signaling ; SOXB1 Transcription Factors - genetics ; SOXB1 Transcription Factors - metabolism ; Spinal cord ; Stem cells ; Transcriptome - drug effects ; Umbilical cord ; Vascular endothelial growth factor</subject><ispartof>PloS one, 2014-07, Vol.9 (7), p.e100576</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Yang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Yang et al 2014 Yang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-1f010cea681f85b5764ae032dbca30bbcd9b18bc81cfb626874ea7a5457817013</citedby><cites>FETCH-LOGICAL-c758t-1f010cea681f85b5764ae032dbca30bbcd9b18bc81cfb626874ea7a5457817013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106758/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106758/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25051057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Chadwick, Brian P.</contributor><creatorcontrib>Yang, Chung-Wei</creatorcontrib><creatorcontrib>Chou, Wei-Chun</creatorcontrib><creatorcontrib>Chen, Kuan-Hsueh</creatorcontrib><creatorcontrib>Cheng, An-Lin</creatorcontrib><creatorcontrib>Mao, I-Fang</creatorcontrib><creatorcontrib>Chao, How-Ran</creatorcontrib><creatorcontrib>Chuang, Chun-Yu</creatorcontrib><title>Visualized gene network reveals the novel target transcripts Sox2 and Pax6 of neuronal development in trans-placental exposure to bisphenol A</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Bisphenol A (BPA) is a ubiquitous endocrine disrupting chemical in our daily life, and its health effect in response to prenatal exposure is still controversial. Early-life BPA exposure may impact brain development and contribute to childhood neurological disorders. The aim of the present study was to investigate molecular target genes of neuronal development in trans-placental exposure to BPA.
A meta-analysis of three public microarray datasets was performed to screen for differentially expressed genes (DEGs) in exposure to BPA. The candidate genes of neuronal development were identified from gene ontology analysis in a reconstructed neuronal sub-network, and their gene expressions were determined using real-time PCR in 20 umbilical cord blood samples dichotomized into high and low BPA level groups upon the median 16.8 nM.
Among 36 neuronal transcripts sorted from DAVID ontology clusters of 457 DEGs using the analysis of Bioconductor limma package, we found two neuronal genes, sex determining region Y-box 2 (Sox2) and paired box 6 (Pax6), had preferentially down-regulated expression (Bonferroni correction p-value <10(-4) and log2-transformed fold change ≤-1.2) in response to BPA exposure. Fetal cord blood samples had the obviously attenuated gene expression of Sox2 and Pax6 in high BPA group referred to low BPA group. Visualized gene network of Cytoscape analysis showed that Sox2 and Pax6 which were contributed to neural precursor cell proliferation and neuronal differentiation might be down-regulated through sonic hedgehog (Shh), vascular endothelial growth factor A (VEGFA) and Notch signaling.
These results indicated that trans-placental BPA exposure down-regulated gene expression of Sox2 and Pax6 potentially underlying the adverse effect on childhood neuronal development.</description><subject>Alcohol</subject><subject>Analysis</subject><subject>Benzhydryl Compounds - toxicity</subject><subject>Biology and Life Sciences</subject><subject>Biomedical engineering</subject><subject>Bisphenol A</subject><subject>Blood</subject><subject>Brain</subject><subject>Cell proliferation</subject><subject>Central Nervous System - drug effects</subject><subject>Central Nervous System - embryology</subject><subject>Children</subject><subject>Cluster analysis</subject><subject>Cord blood</subject><subject>DNA methylation</subject><subject>DNA microarrays</subject><subject>Endocrine disruptors</subject><subject>Endocrine Disruptors - toxicity</subject><subject>Endothelial growth factors</subject><subject>Engineering</subject><subject>Environmental Exposure</subject><subject>Environmental science</subject><subject>Epigenetics</subject><subject>Exposure</subject><subject>Eye Proteins - genetics</subject><subject>Eye Proteins - metabolism</subject><subject>Female</subject><subject>Fetuses</subject><subject>Gene expression</subject><subject>Gene Ontology</subject><subject>Gene Regulatory Networks</subject><subject>Genes</subject><subject>Health aspects</subject><subject>Hedgehog protein</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Hypothesis testing</subject><subject>Insulin</subject><subject>Insulin-like growth factors</subject><subject>Kinases</subject><subject>Maternal-Fetal Exchange</subject><subject>Medicine and Health Sciences</subject><subject>Nervous system diseases</subject><subject>Neurological diseases</subject><subject>Neurons</subject><subject>Notch protein</subject><subject>Paired Box Transcription Factors - genetics</subject><subject>Paired Box Transcription Factors - metabolism</subject><subject>Pax6 protein</subject><subject>PAX6 Transcription Factor</subject><subject>Phenols</subject><subject>Phenols (Class of compounds)</subject><subject>Phenols - toxicity</subject><subject>Placenta</subject><subject>Pregnancy</subject><subject>Pregnant women</subject><subject>Prenatal experience</subject><subject>Prenatal exposure</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Rodents</subject><subject>Signaling</subject><subject>SOXB1 Transcription Factors - genetics</subject><subject>SOXB1 Transcription Factors - metabolism</subject><subject>Spinal cord</subject><subject>Stem cells</subject><subject>Transcriptome - drug effects</subject><subject>Umbilical cord</subject><subject>Vascular endothelial growth factor</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk22L1DAQx4so3nn6DUQDguCLXZM-pNk3wnL4sHBw4um9DWk67ebsJjVJ19Xv4Hd21u0dW1CQvkiZ_P7_zAwzSfKU0TnLSvb6xg3eqm7eOwtzyigtSn4vOWWLLJ3xlGb3j_5Pkkch3CCSCc4fJidpQQuGgtPk17UJg-rMT6hJCxaIhfjd-a_EwxZUF0hcY8xtoSNR-RYiiV7ZoL3pYyBXbpcSZWvyUe04cQ2qB-8wK1KjvHP9Bmwkxh5Es75TGgN4DbvehcEDiY5UJvRrsK4jy8fJgwYfhSfjeZZ8eff28_mH2cXl-9X58mKmy0LEGWuwXg2KC9aIosLCcwU0S-tKq4xWla4XFROVFkw3FU-5KHNQpSryohSspCw7S54ffPvOBTl2MkhW5CVfFAteIrE6ELVTN7L3ZqP8D-mUkX8CzrdS-Wh0BzJjTOlFTqtUVDlr2EKIpgJeZ2mTUp4V6PVmfG2oNlDvW-BVNzGd3lizlq3bypxRjgWjwYvRwLtvA4T4j5RHqlWYlbGNQzO9MUHLZc4ES3kpKFLzv1D41bAxGmepMRifCF5NBMhE2MVWDSHI1dWn_2cvr6fsyyN2jcMW18F1QzTOhimYH0DtXQgemrvOMSr3q3DbDblfBTmuAsqeHXf9TnQ7-9lvdCYGEw</recordid><startdate>20140722</startdate><enddate>20140722</enddate><creator>Yang, Chung-Wei</creator><creator>Chou, Wei-Chun</creator><creator>Chen, Kuan-Hsueh</creator><creator>Cheng, An-Lin</creator><creator>Mao, I-Fang</creator><creator>Chao, How-Ran</creator><creator>Chuang, Chun-Yu</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140722</creationdate><title>Visualized gene network reveals the novel target transcripts Sox2 and Pax6 of neuronal development in trans-placental exposure to bisphenol A</title><author>Yang, Chung-Wei ; Chou, Wei-Chun ; Chen, Kuan-Hsueh ; Cheng, An-Lin ; Mao, I-Fang ; Chao, How-Ran ; Chuang, Chun-Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-1f010cea681f85b5764ae032dbca30bbcd9b18bc81cfb626874ea7a5457817013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Alcohol</topic><topic>Analysis</topic><topic>Benzhydryl Compounds - toxicity</topic><topic>Biology and Life Sciences</topic><topic>Biomedical engineering</topic><topic>Bisphenol A</topic><topic>Blood</topic><topic>Brain</topic><topic>Cell proliferation</topic><topic>Central Nervous System - drug effects</topic><topic>Central Nervous System - embryology</topic><topic>Children</topic><topic>Cluster analysis</topic><topic>Cord blood</topic><topic>DNA methylation</topic><topic>DNA microarrays</topic><topic>Endocrine disruptors</topic><topic>Endocrine Disruptors - toxicity</topic><topic>Endothelial growth factors</topic><topic>Engineering</topic><topic>Environmental Exposure</topic><topic>Environmental science</topic><topic>Epigenetics</topic><topic>Exposure</topic><topic>Eye Proteins - genetics</topic><topic>Eye Proteins - metabolism</topic><topic>Female</topic><topic>Fetuses</topic><topic>Gene expression</topic><topic>Gene Ontology</topic><topic>Gene Regulatory Networks</topic><topic>Genes</topic><topic>Health aspects</topic><topic>Hedgehog protein</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Hypothesis testing</topic><topic>Insulin</topic><topic>Insulin-like growth factors</topic><topic>Kinases</topic><topic>Maternal-Fetal Exchange</topic><topic>Medicine and Health Sciences</topic><topic>Nervous system diseases</topic><topic>Neurological diseases</topic><topic>Neurons</topic><topic>Notch protein</topic><topic>Paired Box Transcription Factors - genetics</topic><topic>Paired Box Transcription Factors - metabolism</topic><topic>Pax6 protein</topic><topic>PAX6 Transcription Factor</topic><topic>Phenols</topic><topic>Phenols (Class of compounds)</topic><topic>Phenols - toxicity</topic><topic>Placenta</topic><topic>Pregnancy</topic><topic>Pregnant women</topic><topic>Prenatal experience</topic><topic>Prenatal exposure</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Rodents</topic><topic>Signaling</topic><topic>SOXB1 Transcription Factors - genetics</topic><topic>SOXB1 Transcription Factors - metabolism</topic><topic>Spinal cord</topic><topic>Stem cells</topic><topic>Transcriptome - drug effects</topic><topic>Umbilical cord</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Chung-Wei</creatorcontrib><creatorcontrib>Chou, Wei-Chun</creatorcontrib><creatorcontrib>Chen, Kuan-Hsueh</creatorcontrib><creatorcontrib>Cheng, An-Lin</creatorcontrib><creatorcontrib>Mao, I-Fang</creatorcontrib><creatorcontrib>Chao, How-Ran</creatorcontrib><creatorcontrib>Chuang, Chun-Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Chung-Wei</au><au>Chou, Wei-Chun</au><au>Chen, Kuan-Hsueh</au><au>Cheng, An-Lin</au><au>Mao, I-Fang</au><au>Chao, How-Ran</au><au>Chuang, Chun-Yu</au><au>Chadwick, Brian P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visualized gene network reveals the novel target transcripts Sox2 and Pax6 of neuronal development in trans-placental exposure to bisphenol A</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-07-22</date><risdate>2014</risdate><volume>9</volume><issue>7</issue><spage>e100576</spage><pages>e100576-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Bisphenol A (BPA) is a ubiquitous endocrine disrupting chemical in our daily life, and its health effect in response to prenatal exposure is still controversial. Early-life BPA exposure may impact brain development and contribute to childhood neurological disorders. The aim of the present study was to investigate molecular target genes of neuronal development in trans-placental exposure to BPA.
A meta-analysis of three public microarray datasets was performed to screen for differentially expressed genes (DEGs) in exposure to BPA. The candidate genes of neuronal development were identified from gene ontology analysis in a reconstructed neuronal sub-network, and their gene expressions were determined using real-time PCR in 20 umbilical cord blood samples dichotomized into high and low BPA level groups upon the median 16.8 nM.
Among 36 neuronal transcripts sorted from DAVID ontology clusters of 457 DEGs using the analysis of Bioconductor limma package, we found two neuronal genes, sex determining region Y-box 2 (Sox2) and paired box 6 (Pax6), had preferentially down-regulated expression (Bonferroni correction p-value <10(-4) and log2-transformed fold change ≤-1.2) in response to BPA exposure. Fetal cord blood samples had the obviously attenuated gene expression of Sox2 and Pax6 in high BPA group referred to low BPA group. Visualized gene network of Cytoscape analysis showed that Sox2 and Pax6 which were contributed to neural precursor cell proliferation and neuronal differentiation might be down-regulated through sonic hedgehog (Shh), vascular endothelial growth factor A (VEGFA) and Notch signaling.
These results indicated that trans-placental BPA exposure down-regulated gene expression of Sox2 and Pax6 potentially underlying the adverse effect on childhood neuronal development.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25051057</pmid><doi>10.1371/journal.pone.0100576</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-07, Vol.9 (7), p.e100576 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1547695967 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Alcohol Analysis Benzhydryl Compounds - toxicity Biology and Life Sciences Biomedical engineering Bisphenol A Blood Brain Cell proliferation Central Nervous System - drug effects Central Nervous System - embryology Children Cluster analysis Cord blood DNA methylation DNA microarrays Endocrine disruptors Endocrine Disruptors - toxicity Endothelial growth factors Engineering Environmental Exposure Environmental science Epigenetics Exposure Eye Proteins - genetics Eye Proteins - metabolism Female Fetuses Gene expression Gene Ontology Gene Regulatory Networks Genes Health aspects Hedgehog protein Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Hypothesis testing Insulin Insulin-like growth factors Kinases Maternal-Fetal Exchange Medicine and Health Sciences Nervous system diseases Neurological diseases Neurons Notch protein Paired Box Transcription Factors - genetics Paired Box Transcription Factors - metabolism Pax6 protein PAX6 Transcription Factor Phenols Phenols (Class of compounds) Phenols - toxicity Placenta Pregnancy Pregnant women Prenatal experience Prenatal exposure Repressor Proteins - genetics Repressor Proteins - metabolism Rodents Signaling SOXB1 Transcription Factors - genetics SOXB1 Transcription Factors - metabolism Spinal cord Stem cells Transcriptome - drug effects Umbilical cord Vascular endothelial growth factor |
| title | Visualized gene network reveals the novel target transcripts Sox2 and Pax6 of neuronal development in trans-placental exposure to bisphenol A |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T23%3A33%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Visualized%20gene%20network%20reveals%20the%20novel%20target%20transcripts%20Sox2%20and%20Pax6%20of%20neuronal%20development%20in%20trans-placental%20exposure%20to%20bisphenol%20A&rft.jtitle=PloS%20one&rft.au=Yang,%20Chung-Wei&rft.date=2014-07-22&rft.volume=9&rft.issue=7&rft.spage=e100576&rft.pages=e100576-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0100576&rft_dat=%3Cgale_plos_%3EA418126780%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1547695967&rft_id=info:pmid/25051057&rft_galeid=A418126780&rft_doaj_id=oai_doaj_org_article_311ac940b28b41f1988fbe6d32f20635&rfr_iscdi=true |