Allyl isothiocyanate ameliorates angiogenesis and inflammation in dextran sulfate sodium-induced acute colitis
Allyl isothiocyanate (AITC) is a phytochemical found in cruciferous vegetables that has known chemopreventive and chemotherapeutic activities. Thus far, the antiangiogenic activity of AITC has not been reported in in vivo studies. Herein, we investigated the effect of AITC on angiogenesis and inflam...
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| description | Allyl isothiocyanate (AITC) is a phytochemical found in cruciferous vegetables that has known chemopreventive and chemotherapeutic activities. Thus far, the antiangiogenic activity of AITC has not been reported in in vivo studies. Herein, we investigated the effect of AITC on angiogenesis and inflammation in a mouse model of colitis. Experimental colitis was induced in mice by administering 3% dextran sulfate sodium via drinking water. To monitor the activity of AITC in this model, we measured body weight, disease activity indices, histopathological scores, microvascular density, myeloperoxidase activity, F4/80 staining, inducible nitric oxide synthase (iNOS) expression, cyclooxygenase-2 (COX-2) expression, and vascular endothelial growth factor (VEGF)-A/VEGF receptor 2 (VEGFR2) expression in the mice. We found that AITC-treated mice showed less weight loss, fewer clinical signs of colitis, and longer colons than vehicle-treated mice. AITC treatment also significantly lessened the disruption of colonic architecture that is normally associated with colitis and repressed the microvascularization response. Further, AITC treatment reduced both leukocyte recruitment and macrophage infiltration into the inflamed colon, and the mechanism these activities involved repressing iNOS and COX-2 expression. Finally, AITC attenuated the expression of VEGF-A and VEGFR2. Thus, AITC may have potential application in treating conditions marked by inflammatory-driven angiogenesis and mucosal inflammation. |
| doi_str_mv | 10.1371/journal.pone.0102975 |
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Thus far, the antiangiogenic activity of AITC has not been reported in in vivo studies. Herein, we investigated the effect of AITC on angiogenesis and inflammation in a mouse model of colitis. Experimental colitis was induced in mice by administering 3% dextran sulfate sodium via drinking water. To monitor the activity of AITC in this model, we measured body weight, disease activity indices, histopathological scores, microvascular density, myeloperoxidase activity, F4/80 staining, inducible nitric oxide synthase (iNOS) expression, cyclooxygenase-2 (COX-2) expression, and vascular endothelial growth factor (VEGF)-A/VEGF receptor 2 (VEGFR2) expression in the mice. We found that AITC-treated mice showed less weight loss, fewer clinical signs of colitis, and longer colons than vehicle-treated mice. AITC treatment also significantly lessened the disruption of colonic architecture that is normally associated with colitis and repressed the microvascularization response. Further, AITC treatment reduced both leukocyte recruitment and macrophage infiltration into the inflamed colon, and the mechanism these activities involved repressing iNOS and COX-2 expression. Finally, AITC attenuated the expression of VEGF-A and VEGFR2. Thus, AITC may have potential application in treating conditions marked by inflammatory-driven angiogenesis and mucosal inflammation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0102975</identifier><identifier>PMID: 25051185</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Allyl isothiocyanate ; Analysis ; Angiogenesis ; Animals ; Antiangiogenics ; Antigens, Differentiation - metabolism ; Biology and Life Sciences ; Blotting, Western ; Body weight ; Body weight loss ; Colitis ; Colitis - chemically induced ; Colitis - metabolism ; Colitis - prevention & control ; Colon ; Colon - drug effects ; Colon - metabolism ; Colon - pathology ; COX-2 inhibitors ; Cyclooxygenase 2 - metabolism ; Cyclooxygenase-2 ; Dextran ; Dextran Sulfate ; Dextrans ; Drinking water ; Food Preservatives - pharmacology ; Fuel consumption ; In vivo methods and tests ; Infiltration ; Inflammation ; Inflammation - metabolism ; Inflammation - prevention & control ; Inflammatory bowel disease ; Isothiocyanate ; Isothiocyanates - pharmacology ; Leukocytes ; Macrophages ; Male ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Microscopy, Confocal ; Microvasculature ; Mucosa ; Neovascularization, Pathologic - metabolism ; Neovascularization, Pathologic - prevention & control ; Nitric oxide ; Nitric Oxide Synthase Type II - metabolism ; Nitric-oxide synthase ; Peroxidase ; Research and Analysis Methods ; Rodents ; Sodium ; Sulfates ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - metabolism ; Vascular Endothelial Growth Factor Receptor-2 - metabolism ; Vegetables</subject><ispartof>PloS one, 2014-07, Vol.9 (7), p.e102975</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Davaatseren et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Davaatseren et al 2014 Davaatseren et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-8c023bb8639eef5998cd7979f3ec0a5459a4e02590d5b3cba0b1a84b25e71f153</citedby><cites>FETCH-LOGICAL-c692t-8c023bb8639eef5998cd7979f3ec0a5459a4e02590d5b3cba0b1a84b25e71f153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106840/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4106840/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25051185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mizoguchi, Emiko</contributor><creatorcontrib>Davaatseren, Munkhtugs</creatorcontrib><creatorcontrib>Hwang, Jin-Taek</creatorcontrib><creatorcontrib>Park, Jae Ho</creatorcontrib><creatorcontrib>Kim, Myung-Sunny</creatorcontrib><creatorcontrib>Wang, Shuaiyu</creatorcontrib><creatorcontrib>Sung, Mi Jeong</creatorcontrib><title>Allyl isothiocyanate ameliorates angiogenesis and inflammation in dextran sulfate sodium-induced acute colitis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Allyl isothiocyanate (AITC) is a phytochemical found in cruciferous vegetables that has known chemopreventive and chemotherapeutic activities. Thus far, the antiangiogenic activity of AITC has not been reported in in vivo studies. Herein, we investigated the effect of AITC on angiogenesis and inflammation in a mouse model of colitis. Experimental colitis was induced in mice by administering 3% dextran sulfate sodium via drinking water. To monitor the activity of AITC in this model, we measured body weight, disease activity indices, histopathological scores, microvascular density, myeloperoxidase activity, F4/80 staining, inducible nitric oxide synthase (iNOS) expression, cyclooxygenase-2 (COX-2) expression, and vascular endothelial growth factor (VEGF)-A/VEGF receptor 2 (VEGFR2) expression in the mice. We found that AITC-treated mice showed less weight loss, fewer clinical signs of colitis, and longer colons than vehicle-treated mice. AITC treatment also significantly lessened the disruption of colonic architecture that is normally associated with colitis and repressed the microvascularization response. Further, AITC treatment reduced both leukocyte recruitment and macrophage infiltration into the inflamed colon, and the mechanism these activities involved repressing iNOS and COX-2 expression. Finally, AITC attenuated the expression of VEGF-A and VEGFR2. Thus, AITC may have potential application in treating conditions marked by inflammatory-driven angiogenesis and mucosal inflammation.</description><subject>Allyl isothiocyanate</subject><subject>Analysis</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Antiangiogenics</subject><subject>Antigens, Differentiation - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Blotting, Western</subject><subject>Body weight</subject><subject>Body weight loss</subject><subject>Colitis</subject><subject>Colitis - chemically induced</subject><subject>Colitis - metabolism</subject><subject>Colitis - prevention & control</subject><subject>Colon</subject><subject>Colon - drug effects</subject><subject>Colon - metabolism</subject><subject>Colon - pathology</subject><subject>COX-2 inhibitors</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Cyclooxygenase-2</subject><subject>Dextran</subject><subject>Dextran Sulfate</subject><subject>Dextrans</subject><subject>Drinking water</subject><subject>Food Preservatives - pharmacology</subject><subject>Fuel consumption</subject><subject>In vivo methods and tests</subject><subject>Infiltration</subject><subject>Inflammation</subject><subject>Inflammation - metabolism</subject><subject>Inflammation - prevention & control</subject><subject>Inflammatory bowel disease</subject><subject>Isothiocyanate</subject><subject>Isothiocyanates - pharmacology</subject><subject>Leukocytes</subject><subject>Macrophages</subject><subject>Male</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microscopy, Confocal</subject><subject>Microvasculature</subject><subject>Mucosa</subject><subject>Neovascularization, Pathologic - metabolism</subject><subject>Neovascularization, Pathologic - prevention & control</subject><subject>Nitric oxide</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>Nitric-oxide synthase</subject><subject>Peroxidase</subject><subject>Research and Analysis Methods</subject><subject>Rodents</subject><subject>Sodium</subject><subject>Sulfates</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>Vascular Endothelial Growth Factor Receptor-2 - metabolism</subject><subject>Vegetables</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QLguDFjEmatMmNMCx-DCws-HUbTpO0kyFNxqYV59-bOt1lCgqSiyQnz_smnLxZ9hyjNS4q_HYfxt6DWx-CN2uEEREVe5BdYlGQVUlQ8fBsfZE9iXGPECt4WT7OLghDDGPOLjO_ce7ochvDsLNBHcHDYHLojLOhT8uYg29taI030U4bnVvfOOg6GGzwaZNr82vowedxdM0kjkHbsVtZr0dldA5qTEUVnB1sfJo9asBF82yer7JvH95_vf60urn9uL3e3KxUKciw4gqRoq55WQhjGiYEV7oSlWgKoxAwygRQgwgTSLO6UDWgGgOnNWGmwg1mxVX28uR7cCHKuVVRYkarUjDBJmJ7InSAvTz0toP-KANY-acQ-lZCP1jljKx4QTQtteEcKFEaalqmixGpamVorZPXu_m2se6MVsanhriF6fLE251sw09JMSo5Rcng1WzQhx-jicM_njxTLaRXpW8IyUx1Niq5oZhjUpacJ2r9FyoNbTqrUlgam-oLwZuFIDFD-tIWxhjl9svn_2dvvy_Z12fszoAbdjG4ccpNXIL0BKo-xNib5r5zGMkp63fdkFPW5Zz1JHtx3vV70V24i99Iq_w8</recordid><startdate>20140722</startdate><enddate>20140722</enddate><creator>Davaatseren, Munkhtugs</creator><creator>Hwang, Jin-Taek</creator><creator>Park, Jae Ho</creator><creator>Kim, Myung-Sunny</creator><creator>Wang, Shuaiyu</creator><creator>Sung, Mi Jeong</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140722</creationdate><title>Allyl isothiocyanate ameliorates angiogenesis and inflammation in dextran sulfate sodium-induced acute colitis</title><author>Davaatseren, Munkhtugs ; Hwang, Jin-Taek ; Park, Jae Ho ; Kim, Myung-Sunny ; Wang, Shuaiyu ; Sung, Mi Jeong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-8c023bb8639eef5998cd7979f3ec0a5459a4e02590d5b3cba0b1a84b25e71f153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Allyl isothiocyanate</topic><topic>Analysis</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Antiangiogenics</topic><topic>Antigens, Differentiation - metabolism</topic><topic>Biology and Life Sciences</topic><topic>Blotting, Western</topic><topic>Body weight</topic><topic>Body weight loss</topic><topic>Colitis</topic><topic>Colitis - chemically induced</topic><topic>Colitis - metabolism</topic><topic>Colitis - prevention & control</topic><topic>Colon</topic><topic>Colon - drug effects</topic><topic>Colon - metabolism</topic><topic>Colon - pathology</topic><topic>COX-2 inhibitors</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Cyclooxygenase-2</topic><topic>Dextran</topic><topic>Dextran Sulfate</topic><topic>Dextrans</topic><topic>Drinking water</topic><topic>Food Preservatives - pharmacology</topic><topic>Fuel consumption</topic><topic>In vivo methods and tests</topic><topic>Infiltration</topic><topic>Inflammation</topic><topic>Inflammation - metabolism</topic><topic>Inflammation - prevention & control</topic><topic>Inflammatory bowel disease</topic><topic>Isothiocyanate</topic><topic>Isothiocyanates - pharmacology</topic><topic>Leukocytes</topic><topic>Macrophages</topic><topic>Male</topic><topic>Medicine and Health Sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microscopy, Confocal</topic><topic>Microvasculature</topic><topic>Mucosa</topic><topic>Neovascularization, Pathologic - 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Thus far, the antiangiogenic activity of AITC has not been reported in in vivo studies. Herein, we investigated the effect of AITC on angiogenesis and inflammation in a mouse model of colitis. Experimental colitis was induced in mice by administering 3% dextran sulfate sodium via drinking water. To monitor the activity of AITC in this model, we measured body weight, disease activity indices, histopathological scores, microvascular density, myeloperoxidase activity, F4/80 staining, inducible nitric oxide synthase (iNOS) expression, cyclooxygenase-2 (COX-2) expression, and vascular endothelial growth factor (VEGF)-A/VEGF receptor 2 (VEGFR2) expression in the mice. We found that AITC-treated mice showed less weight loss, fewer clinical signs of colitis, and longer colons than vehicle-treated mice. AITC treatment also significantly lessened the disruption of colonic architecture that is normally associated with colitis and repressed the microvascularization response. Further, AITC treatment reduced both leukocyte recruitment and macrophage infiltration into the inflamed colon, and the mechanism these activities involved repressing iNOS and COX-2 expression. Finally, AITC attenuated the expression of VEGF-A and VEGFR2. Thus, AITC may have potential application in treating conditions marked by inflammatory-driven angiogenesis and mucosal inflammation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25051185</pmid><doi>10.1371/journal.pone.0102975</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-07, Vol.9 (7), p.e102975 |
| issn | 1932-6203 1932-6203 |
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| recordid | cdi_plos_journals_1547695955 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Allyl isothiocyanate Analysis Angiogenesis Animals Antiangiogenics Antigens, Differentiation - metabolism Biology and Life Sciences Blotting, Western Body weight Body weight loss Colitis Colitis - chemically induced Colitis - metabolism Colitis - prevention & control Colon Colon - drug effects Colon - metabolism Colon - pathology COX-2 inhibitors Cyclooxygenase 2 - metabolism Cyclooxygenase-2 Dextran Dextran Sulfate Dextrans Drinking water Food Preservatives - pharmacology Fuel consumption In vivo methods and tests Infiltration Inflammation Inflammation - metabolism Inflammation - prevention & control Inflammatory bowel disease Isothiocyanate Isothiocyanates - pharmacology Leukocytes Macrophages Male Medicine and Health Sciences Mice Mice, Inbred C57BL Microscopy, Confocal Microvasculature Mucosa Neovascularization, Pathologic - metabolism Neovascularization, Pathologic - prevention & control Nitric oxide Nitric Oxide Synthase Type II - metabolism Nitric-oxide synthase Peroxidase Research and Analysis Methods Rodents Sodium Sulfates Vascular endothelial growth factor Vascular Endothelial Growth Factor A - metabolism Vascular Endothelial Growth Factor Receptor-2 - metabolism Vegetables |
| title | Allyl isothiocyanate ameliorates angiogenesis and inflammation in dextran sulfate sodium-induced acute colitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T12%3A08%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Allyl%20isothiocyanate%20ameliorates%20angiogenesis%20and%20inflammation%20in%20dextran%20sulfate%20sodium-induced%20acute%20colitis&rft.jtitle=PloS%20one&rft.au=Davaatseren,%20Munkhtugs&rft.date=2014-07-22&rft.volume=9&rft.issue=7&rft.spage=e102975&rft.pages=e102975-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0102975&rft_dat=%3Cgale_plos_%3EA418126688%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1547695955&rft_id=info:pmid/25051185&rft_galeid=A418126688&rft_doaj_id=oai_doaj_org_article_7832d46de88a42cdab460d5027bce4bd&rfr_iscdi=true |