Microneedle enhanced delivery of cosmeceutically relevant peptides in human skin
Peptides and proteins play an important role in skin health and well-being. They are also found to contribute to skin aging and melanogenesis. Microneedles have been shown to substantially enhance skin penetration and may offer an effective means of peptide delivery enhancement. The aim of this inve...
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| Veröffentlicht in: | PloS one 2014-07, Vol.9 (7), p.e101956-e101956 |
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| creator | Mohammed, Yousuf H Yamada, Miko Lin, Lynlee L Grice, Jeffrey E Roberts, Michael S Raphael, Anthony P Benson, Heather A E Prow, Tarl W |
| description | Peptides and proteins play an important role in skin health and well-being. They are also found to contribute to skin aging and melanogenesis. Microneedles have been shown to substantially enhance skin penetration and may offer an effective means of peptide delivery enhancement. The aim of this investigation was to assess the influence of microneedles on the skin penetration of peptides using fluorescence imaging to determine skin distribution. In particular the effect of peptide chain length (3, 4, 5 amino acid chain length) on passive and MN facilitated skin penetration was investigated. Confocal laser scanning microscopy was used to image fluorescence intensity and the area of penetration of fluorescently tagged peptides. Penetration studies were conducted on excised full thickness human skin in Franz type diffusion cells for 1 and 24 hours. A 2 to 22 fold signal improvement in microneedle enhanced delivery of melanostatin, rigin and pal-KTTKS was observed. To our knowledge this is the first description of microneedle enhanced skin permeation studies on these peptides. |
| doi_str_mv | 10.1371/journal.pone.0101956 |
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R. B.</contributor><creatorcontrib>Mohammed, Yousuf H ; Yamada, Miko ; Lin, Lynlee L ; Grice, Jeffrey E ; Roberts, Michael S ; Raphael, Anthony P ; Benson, Heather A E ; Prow, Tarl W ; Castanho, Miguel A. R. B.</creatorcontrib><description>Peptides and proteins play an important role in skin health and well-being. They are also found to contribute to skin aging and melanogenesis. Microneedles have been shown to substantially enhance skin penetration and may offer an effective means of peptide delivery enhancement. The aim of this investigation was to assess the influence of microneedles on the skin penetration of peptides using fluorescence imaging to determine skin distribution. In particular the effect of peptide chain length (3, 4, 5 amino acid chain length) on passive and MN facilitated skin penetration was investigated. Confocal laser scanning microscopy was used to image fluorescence intensity and the area of penetration of fluorescently tagged peptides. Penetration studies were conducted on excised full thickness human skin in Franz type diffusion cells for 1 and 24 hours. A 2 to 22 fold signal improvement in microneedle enhanced delivery of melanostatin, rigin and pal-KTTKS was observed. To our knowledge this is the first description of microneedle enhanced skin permeation studies on these peptides.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0101956</identifier><identifier>PMID: 25033398</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abdominoplasty ; Administration, Cutaneous ; Aging ; Amino acids ; Cell adhesion & migration ; Chains ; Confocal microscopy ; Cosmetic Techniques ; Dermatology ; Diffusion cells ; Drug Delivery Systems - methods ; Fluorescence ; Humans ; Inflammation ; Medical research ; Medicine ; Medicine and Health Sciences ; Microinjections - methods ; Microscopy ; Microscopy, Confocal ; MSH Release-Inhibiting Hormone - administration & dosage ; Needles ; Nonsteroidal anti-inflammatory drugs ; Oligopeptides - administration & dosage ; Optical Imaging ; Penetration ; Peptides ; Peptides - administration & dosage ; Peptides - therapeutic use ; Permeability ; Pharmaceutical sciences ; Pharmacy ; Proteins ; Researchers ; Scanning microscopy ; Skin ; Skin Physiological Phenomena ; Studies ; Surgery, Plastic - methods ; Transdermal medication ; Well being</subject><ispartof>PloS one, 2014-07, Vol.9 (7), p.e101956-e101956</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Mohammed et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Mohammed et al 2014 Mohammed et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-161a4da18c949a3e5e402347e3f5a77e48ead50045e7f398ffe46c8fc7f2dc173</citedby><cites>FETCH-LOGICAL-c692t-161a4da18c949a3e5e402347e3f5a77e48ead50045e7f398ffe46c8fc7f2dc173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102460/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4102460/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25033398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Castanho, Miguel A. R. B.</contributor><creatorcontrib>Mohammed, Yousuf H</creatorcontrib><creatorcontrib>Yamada, Miko</creatorcontrib><creatorcontrib>Lin, Lynlee L</creatorcontrib><creatorcontrib>Grice, Jeffrey E</creatorcontrib><creatorcontrib>Roberts, Michael S</creatorcontrib><creatorcontrib>Raphael, Anthony P</creatorcontrib><creatorcontrib>Benson, Heather A E</creatorcontrib><creatorcontrib>Prow, Tarl W</creatorcontrib><title>Microneedle enhanced delivery of cosmeceutically relevant peptides in human skin</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Peptides and proteins play an important role in skin health and well-being. They are also found to contribute to skin aging and melanogenesis. Microneedles have been shown to substantially enhance skin penetration and may offer an effective means of peptide delivery enhancement. The aim of this investigation was to assess the influence of microneedles on the skin penetration of peptides using fluorescence imaging to determine skin distribution. In particular the effect of peptide chain length (3, 4, 5 amino acid chain length) on passive and MN facilitated skin penetration was investigated. Confocal laser scanning microscopy was used to image fluorescence intensity and the area of penetration of fluorescently tagged peptides. Penetration studies were conducted on excised full thickness human skin in Franz type diffusion cells for 1 and 24 hours. A 2 to 22 fold signal improvement in microneedle enhanced delivery of melanostatin, rigin and pal-KTTKS was observed. To our knowledge this is the first description of microneedle enhanced skin permeation studies on these peptides.</description><subject>Abdominoplasty</subject><subject>Administration, Cutaneous</subject><subject>Aging</subject><subject>Amino acids</subject><subject>Cell adhesion & migration</subject><subject>Chains</subject><subject>Confocal microscopy</subject><subject>Cosmetic Techniques</subject><subject>Dermatology</subject><subject>Diffusion cells</subject><subject>Drug Delivery Systems - methods</subject><subject>Fluorescence</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Microinjections - methods</subject><subject>Microscopy</subject><subject>Microscopy, Confocal</subject><subject>MSH Release-Inhibiting Hormone - administration & dosage</subject><subject>Needles</subject><subject>Nonsteroidal anti-inflammatory drugs</subject><subject>Oligopeptides - 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In particular the effect of peptide chain length (3, 4, 5 amino acid chain length) on passive and MN facilitated skin penetration was investigated. Confocal laser scanning microscopy was used to image fluorescence intensity and the area of penetration of fluorescently tagged peptides. Penetration studies were conducted on excised full thickness human skin in Franz type diffusion cells for 1 and 24 hours. A 2 to 22 fold signal improvement in microneedle enhanced delivery of melanostatin, rigin and pal-KTTKS was observed. To our knowledge this is the first description of microneedle enhanced skin permeation studies on these peptides.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25033398</pmid><doi>10.1371/journal.pone.0101956</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abdominoplasty Administration, Cutaneous Aging Amino acids Cell adhesion & migration Chains Confocal microscopy Cosmetic Techniques Dermatology Diffusion cells Drug Delivery Systems - methods Fluorescence Humans Inflammation Medical research Medicine Medicine and Health Sciences Microinjections - methods Microscopy Microscopy, Confocal MSH Release-Inhibiting Hormone - administration & dosage Needles Nonsteroidal anti-inflammatory drugs Oligopeptides - administration & dosage Optical Imaging Penetration Peptides Peptides - administration & dosage Peptides - therapeutic use Permeability Pharmaceutical sciences Pharmacy Proteins Researchers Scanning microscopy Skin Skin Physiological Phenomena Studies Surgery, Plastic - methods Transdermal medication Well being |
| title | Microneedle enhanced delivery of cosmeceutically relevant peptides in human skin |
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