MicroRNA-152 regulates DNA methyltransferase 1 and is involved in the development and lactation of mammary glands in dairy cows
MicroRNAs (miRNAs) are a class of small non-coding, endogenous regulatory RNAs that function by controlling gene expression at the post-transcriptional level. Using small RNA sequencing and qRT-PCR techniques, we found that the expression of miR-152 was significantly increased during lactation in th...
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| creator | Wang, Jie Bian, Yanjie Wang, Zhuoran Li, Dan Wang, Chunmei Li, Qingzhang Gao, Xuejun |
| description | MicroRNAs (miRNAs) are a class of small non-coding, endogenous regulatory RNAs that function by controlling gene expression at the post-transcriptional level. Using small RNA sequencing and qRT-PCR techniques, we found that the expression of miR-152 was significantly increased during lactation in the mammary glands of dairy cows producing high quality milk compared with that in cows producing low quality milk. Furthermore, DNA methyltransferase 1 (DNMT1), which is a target of miR-152, was inversely correlated with the expression levels of miR-152 in the mammary glands of dairy cows. Dairy cow mammary epithelial cells (DCMECs) were used as in vitro cell models to study the function of miR-152. The forced expression of miR-152 in DCMECs resulted in a marked reduction of DNMT1 at both mRNA and protein levels. This in turn led to a decrease in global DNA methylation and increased the expression of two lactation-related genes, serine/threonine protein kinase Akt (Akt) and peroxisome proliferator-activated receptor gamma (Pparγ). In contrast, inhibition of miR-152 showed the opposite results. By using an electronic Coulter counter (CASY-TT) and flow cytometer, we discovered that miR-152 enhanced the viability and multiplication capacity of DCMECs. In conclusion, miR-152 plays an important role in the development and lactation processes in the mammary glands of dairy cows. Our data provide insights into dairy cow mammary gland development and lactation. |
| doi_str_mv | 10.1371/journal.pone.0101358 |
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Using small RNA sequencing and qRT-PCR techniques, we found that the expression of miR-152 was significantly increased during lactation in the mammary glands of dairy cows producing high quality milk compared with that in cows producing low quality milk. Furthermore, DNA methyltransferase 1 (DNMT1), which is a target of miR-152, was inversely correlated with the expression levels of miR-152 in the mammary glands of dairy cows. Dairy cow mammary epithelial cells (DCMECs) were used as in vitro cell models to study the function of miR-152. The forced expression of miR-152 in DCMECs resulted in a marked reduction of DNMT1 at both mRNA and protein levels. This in turn led to a decrease in global DNA methylation and increased the expression of two lactation-related genes, serine/threonine protein kinase Akt (Akt) and peroxisome proliferator-activated receptor gamma (Pparγ). In contrast, inhibition of miR-152 showed the opposite results. By using an electronic Coulter counter (CASY-TT) and flow cytometer, we discovered that miR-152 enhanced the viability and multiplication capacity of DCMECs. In conclusion, miR-152 plays an important role in the development and lactation processes in the mammary glands of dairy cows. Our data provide insights into dairy cow mammary gland development and lactation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0101358</identifier><identifier>PMID: 24987964</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>AKT protein ; Animals ; Biology and life sciences ; Breasts ; Caseins - metabolism ; Cattle ; Cell culture ; Cell Line ; Comparative analysis ; Dairy cattle ; Deoxyribonucleic acid ; DNA ; DNA (Cytosine-5-)-Methyltransferase 1 ; DNA (Cytosine-5-)-Methyltransferases - genetics ; DNA (Cytosine-5-)-Methyltransferases - metabolism ; DNA Methylation ; DNA methyltransferase ; DNMT1 protein ; Enzyme Activation ; Epithelial cells ; Epithelial Cells - metabolism ; Female ; Gene expression ; Gene Expression Regulation ; Gene Expression Regulation, Enzymologic ; Gene sequencing ; Genomics ; Glands ; Goats ; HEK293 Cells ; Humans ; Lactation ; Lactose - metabolism ; Mammary gland ; Mammary glands ; Mammary Glands, Animal - cytology ; Mammary Glands, Animal - physiology ; Methylation ; MicroRNA ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Post-transcription ; PPAR gamma - genetics ; Protein kinase ; Protein-serine/threonine kinase ; Proto-Oncogene Proteins c-akt - genetics ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; Threonine ; Transferases ; Triglycerides - metabolism ; Viability</subject><ispartof>PloS one, 2014-07, Vol.9 (7), p.e101358-e101358</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Wang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Wang et al 2014 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6078-29e893adc1be99b0e7b69ad9849d009d9eb09bd3598e65ef2d233e55763a0b693</citedby><cites>FETCH-LOGICAL-c6078-29e893adc1be99b0e7b69ad9849d009d9eb09bd3598e65ef2d233e55763a0b693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079547/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4079547/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24987964$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Chadwick, Brian P.</contributor><creatorcontrib>Wang, Jie</creatorcontrib><creatorcontrib>Bian, Yanjie</creatorcontrib><creatorcontrib>Wang, Zhuoran</creatorcontrib><creatorcontrib>Li, Dan</creatorcontrib><creatorcontrib>Wang, Chunmei</creatorcontrib><creatorcontrib>Li, Qingzhang</creatorcontrib><creatorcontrib>Gao, Xuejun</creatorcontrib><title>MicroRNA-152 regulates DNA methyltransferase 1 and is involved in the development and lactation of mammary glands in dairy cows</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>MicroRNAs (miRNAs) are a class of small non-coding, endogenous regulatory RNAs that function by controlling gene expression at the post-transcriptional level. Using small RNA sequencing and qRT-PCR techniques, we found that the expression of miR-152 was significantly increased during lactation in the mammary glands of dairy cows producing high quality milk compared with that in cows producing low quality milk. Furthermore, DNA methyltransferase 1 (DNMT1), which is a target of miR-152, was inversely correlated with the expression levels of miR-152 in the mammary glands of dairy cows. Dairy cow mammary epithelial cells (DCMECs) were used as in vitro cell models to study the function of miR-152. The forced expression of miR-152 in DCMECs resulted in a marked reduction of DNMT1 at both mRNA and protein levels. This in turn led to a decrease in global DNA methylation and increased the expression of two lactation-related genes, serine/threonine protein kinase Akt (Akt) and peroxisome proliferator-activated receptor gamma (Pparγ). In contrast, inhibition of miR-152 showed the opposite results. By using an electronic Coulter counter (CASY-TT) and flow cytometer, we discovered that miR-152 enhanced the viability and multiplication capacity of DCMECs. In conclusion, miR-152 plays an important role in the development and lactation processes in the mammary glands of dairy cows. Our data provide insights into dairy cow mammary gland development and lactation.</description><subject>AKT protein</subject><subject>Animals</subject><subject>Biology and life sciences</subject><subject>Breasts</subject><subject>Caseins - metabolism</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>Comparative analysis</subject><subject>Dairy cattle</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA (Cytosine-5-)-Methyltransferase 1</subject><subject>DNA (Cytosine-5-)-Methyltransferases - genetics</subject><subject>DNA (Cytosine-5-)-Methyltransferases - metabolism</subject><subject>DNA Methylation</subject><subject>DNA methyltransferase</subject><subject>DNMT1 protein</subject><subject>Enzyme Activation</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Gene sequencing</subject><subject>Genomics</subject><subject>Glands</subject><subject>Goats</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Lactation</subject><subject>Lactose - metabolism</subject><subject>Mammary gland</subject><subject>Mammary glands</subject><subject>Mammary Glands, Animal - cytology</subject><subject>Mammary Glands, Animal - physiology</subject><subject>Methylation</subject><subject>MicroRNA</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Post-transcription</subject><subject>PPAR gamma - genetics</subject><subject>Protein kinase</subject><subject>Protein-serine/threonine kinase</subject><subject>Proto-Oncogene Proteins c-akt - genetics</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>Threonine</subject><subject>Transferases</subject><subject>Triglycerides - metabolism</subject><subject>Viability</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk01v1DAQhiMEoqXwDxBYQkJw2MWO82FfkFbla6XSSuXjajn2ZNcrJ97ayUJP_HWcblptUA8ohzj2877jmcwkyXOC54SW5N3G9b6Vdr51LcwxwYTm7EFyTDhNZ0WK6cOD9VHyJIQNxjllRfE4OUozzkpeZMfJn69GeXd5vpiRPEUeVr2VHQT04XyBGujW17bzsg01eBkAESRbjUxApt05u4O4blG3BqRhB9ZtG2i7G8RK1cnOuBa5GjWyaaS_RisbjwYt0tLEb-V-hafJo1raAM_G90ny49PH76dfZmcXn5eni7OZKnDJZikHxqnUilTAeYWhrAouNWcZ1xhzzaHCvNI05wyKHOpUp5RCnpcFlTii9CR5uffdWhfEWLsgSJ7RjPE0ZZFY7gnt5EZsvRnuLJw04mbD-ZWQvjPKgmBVyRSp07qMaoWlxLpSRcELXaYVzrLo9X6M1lcNaBXL4qWdmE5PWrMWK7cTGS55npXR4M1o4N1VD6ETjQkKbKwguH5_75RhwofMXv2D3p_dSK1kTMC0tYtx1WAqFhkpSkYZGcLO76Hio6ExKjZabeL-RPB2IohMB7-7lexDEMtvl__PXvycsq8P2DVI262Ds_3QUmEKZnswdnEIHuq7IhMshjm5rYYY5kSMcxJlLw5_0J3odjDoX-68DT0</recordid><startdate>20140702</startdate><enddate>20140702</enddate><creator>Wang, Jie</creator><creator>Bian, Yanjie</creator><creator>Wang, Zhuoran</creator><creator>Li, Dan</creator><creator>Wang, Chunmei</creator><creator>Li, Qingzhang</creator><creator>Gao, Xuejun</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140702</creationdate><title>MicroRNA-152 regulates DNA methyltransferase 1 and is involved in the development and lactation of mammary glands in dairy cows</title><author>Wang, Jie ; Bian, Yanjie ; Wang, Zhuoran ; Li, Dan ; Wang, Chunmei ; Li, Qingzhang ; Gao, Xuejun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6078-29e893adc1be99b0e7b69ad9849d009d9eb09bd3598e65ef2d233e55763a0b693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>AKT protein</topic><topic>Animals</topic><topic>Biology and life sciences</topic><topic>Breasts</topic><topic>Caseins - metabolism</topic><topic>Cattle</topic><topic>Cell culture</topic><topic>Cell Line</topic><topic>Comparative analysis</topic><topic>Dairy cattle</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA (Cytosine-5-)-Methyltransferase 1</topic><topic>DNA (Cytosine-5-)-Methyltransferases - genetics</topic><topic>DNA (Cytosine-5-)-Methyltransferases - metabolism</topic><topic>DNA Methylation</topic><topic>DNA methyltransferase</topic><topic>DNMT1 protein</topic><topic>Enzyme Activation</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gene sequencing</topic><topic>Genomics</topic><topic>Glands</topic><topic>Goats</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Lactation</topic><topic>Lactose - metabolism</topic><topic>Mammary gland</topic><topic>Mammary glands</topic><topic>Mammary Glands, Animal - 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Using small RNA sequencing and qRT-PCR techniques, we found that the expression of miR-152 was significantly increased during lactation in the mammary glands of dairy cows producing high quality milk compared with that in cows producing low quality milk. Furthermore, DNA methyltransferase 1 (DNMT1), which is a target of miR-152, was inversely correlated with the expression levels of miR-152 in the mammary glands of dairy cows. Dairy cow mammary epithelial cells (DCMECs) were used as in vitro cell models to study the function of miR-152. The forced expression of miR-152 in DCMECs resulted in a marked reduction of DNMT1 at both mRNA and protein levels. This in turn led to a decrease in global DNA methylation and increased the expression of two lactation-related genes, serine/threonine protein kinase Akt (Akt) and peroxisome proliferator-activated receptor gamma (Pparγ). In contrast, inhibition of miR-152 showed the opposite results. By using an electronic Coulter counter (CASY-TT) and flow cytometer, we discovered that miR-152 enhanced the viability and multiplication capacity of DCMECs. In conclusion, miR-152 plays an important role in the development and lactation processes in the mammary glands of dairy cows. Our data provide insights into dairy cow mammary gland development and lactation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24987964</pmid><doi>10.1371/journal.pone.0101358</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | AKT protein Animals Biology and life sciences Breasts Caseins - metabolism Cattle Cell culture Cell Line Comparative analysis Dairy cattle Deoxyribonucleic acid DNA DNA (Cytosine-5-)-Methyltransferase 1 DNA (Cytosine-5-)-Methyltransferases - genetics DNA (Cytosine-5-)-Methyltransferases - metabolism DNA Methylation DNA methyltransferase DNMT1 protein Enzyme Activation Epithelial cells Epithelial Cells - metabolism Female Gene expression Gene Expression Regulation Gene Expression Regulation, Enzymologic Gene sequencing Genomics Glands Goats HEK293 Cells Humans Lactation Lactose - metabolism Mammary gland Mammary glands Mammary Glands, Animal - cytology Mammary Glands, Animal - physiology Methylation MicroRNA MicroRNAs - genetics MicroRNAs - metabolism miRNA Post-transcription PPAR gamma - genetics Protein kinase Protein-serine/threonine kinase Proto-Oncogene Proteins c-akt - genetics Ribonucleic acid RNA RNA, Messenger - genetics Threonine Transferases Triglycerides - metabolism Viability |
| title | MicroRNA-152 regulates DNA methyltransferase 1 and is involved in the development and lactation of mammary glands in dairy cows |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T19%3A33%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=MicroRNA-152%20regulates%20DNA%20methyltransferase%201%20and%20is%20involved%20in%20the%20development%20and%20lactation%20of%20mammary%20glands%20in%20dairy%20cows&rft.jtitle=PloS%20one&rft.au=Wang,%20Jie&rft.date=2014-07-02&rft.volume=9&rft.issue=7&rft.spage=e101358&rft.epage=e101358&rft.pages=e101358-e101358&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0101358&rft_dat=%3Cgale_plos_%3EA416783817%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1543489228&rft_id=info:pmid/24987964&rft_galeid=A416783817&rft_doaj_id=oai_doaj_org_article_8b78c1f2f7434c0aa0dbc6696d72b044&rfr_iscdi=true |