Galactose oxidase from Fusarium oxysporum--expression in E. coli and P. pastoris and biochemical characterization

A gene coding for galactose 6-oxidase from Fusarium oxysporum G12 was cloned together with its native preprosequence and a C-terminal His-tag, and successfully expressed both in Escherichia coli and Pichia pastoris. The enzyme was subsequently purified and characterized. Among all tested substrates,...

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Veröffentlicht in:	PloS one 2014-06, Vol.9 (6), p.e100116-e100116
Hauptverfasser:	Paukner, Regina, Staudigl, Petra, Choosri, Withu, Sygmund, Christoph, Halada, Petr, Haltrich, Dietmar, Leitner, Christian
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Biology and Life Sciences Catalysis Catalytic activity Cloning Cloning, Molecular Coordination compounds Copper Crosslinking D-Galactose Deoxyribonucleic acid DNA E coli Enzymatic activity Enzyme activity Enzymes Escherichia coli Escherichia coli - genetics Ethers - chemistry Food science Fusarium Fusarium - enzymology Fusarium - genetics Fusarium graminearum Fusarium oxysporum Galactose Galactose oxidase Galactose Oxidase - chemistry Galactose Oxidase - genetics Galactose Oxidase - isolation & purification Galactose Oxidase - metabolism Gene Expression Hydrogen-Ion Concentration Life sciences Mass spectrometry Mass spectroscopy Models, Molecular Molecular Sequence Data Natural resources Oxidase Oxidases pH effects Physical Sciences Pichia - genetics Pichia pastoris Protein Conformation Proteins Substrates Sulfur Tyrosine Yeast
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