Photoactivated localization microscopy with bimolecular fluorescence complementation (BiFC-PALM) for nanoscale imaging of protein-protein interactions in cells

Bimolecular fluorescence complementation (BiFC) has been widely used to visualize protein-protein interactions (PPIs) in cells. Until now, however, the resolution of BiFC has been limited by the diffraction of light to ∼250 nm, much larger than the nanometer scale at which PPIs occur or are regulate...

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Veröffentlicht in:	PloS one 2014-06, Vol.9 (6), p.e100589-e100589
Hauptverfasser:	Nickerson, Andrew, Huang, Tao, Lin, Li-Jung, Nan, Xiaolin
Format:	Artikel
Sprache:	eng
Schlagworte:	Biology and Life Sciences Biomedical engineering Cancer Cell Line, Tumor Cloning Clustering Engineering Fluorescence Fluorescence resonance energy transfer Guanosine triphosphatases Guanosinetriphosphatase Humans Imaging Imaging techniques K-Ras protein Light Light diffraction Localization Luminescent Proteins - chemistry Luminescent Proteins - metabolism Microscopy Microscopy, Fluorescence - methods Models, Molecular Nanotechnology - methods Palm Plasmids Protein interaction Protein Structure, Secondary Protein-protein interactions Proteins Research and Analysis Methods Science Spatial discrimination Spatial resolution
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