Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold
Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D pr...
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| Veröffentlicht in: | PloS one 2014-06, Vol.9 (6), p.e99410-e99410 |
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| creator | Ye, Ken Felimban, Raed Traianedes, Kathy Moulton, Simon E Wallace, Gordon G Chung, Johnson Quigley, Anita Choong, Peter F M Myers, Damian E |
| description | Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, a pearlescent, cartilage-like matrix had formed that penetrated the top layers of the chitosan scaffold forming a 'cap' on the scaffold. Chondrocytic morphology showed typical cells encased in extracellular matrix which stained positively with toluidine blue. Immunohistochemistry demonstrated positive staining for collagen type II and cartilage proteoglycans, as well as collagen type I. Real time PCR analysis showed up-regulation of collagen type II, aggrecan and SOX9 genes when IPFP-ASCs were stimulated by TGFβ3 and BMP6. Thus, IPFP-ASCs can successfully undergo chondrogenesis using TGFβ3 and BMP6 and the cartilage-like tissue that forms on the surface of 3D-printed chitosan scaffold may prove useful as an osteochondral graft. |
| doi_str_mv | 10.1371/journal.pone.0099410 |
| format | Article |
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When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, a pearlescent, cartilage-like matrix had formed that penetrated the top layers of the chitosan scaffold forming a 'cap' on the scaffold. Chondrocytic morphology showed typical cells encased in extracellular matrix which stained positively with toluidine blue. Immunohistochemistry demonstrated positive staining for collagen type II and cartilage proteoglycans, as well as collagen type I. Real time PCR analysis showed up-regulation of collagen type II, aggrecan and SOX9 genes when IPFP-ASCs were stimulated by TGFβ3 and BMP6. Thus, IPFP-ASCs can successfully undergo chondrogenesis using TGFβ3 and BMP6 and the cartilage-like tissue that forms on the surface of 3D-printed chitosan scaffold may prove useful as an osteochondral graft.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0099410</identifier><identifier>PMID: 24918443</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipose Tissue - cytology ; Aggrecan ; Analysis ; Arthritis ; Biocompatibility ; Biology and Life Sciences ; Biomedical materials ; Bone marrow ; Bone morphogenetic protein 6 ; Bone morphogenetic proteins ; Cartilage ; Casing (process) ; Chitosan ; Chondrogenesis ; Collagen ; Collagen (type I) ; Collagen (type II) ; Defects ; Engineering and Technology ; Ethics ; Extracellular matrix ; Gene expression ; Growth factors ; Humans ; Immunohistochemistry ; Joint surgery ; Knee ; Medicine and Health Sciences ; Neurosciences ; Patella - cytology ; Physical Sciences ; Polymers ; Proteoglycans ; Real-Time Polymerase Chain Reaction ; Scaffolds ; Sox9 protein ; Stem cells ; Stem Cells - cytology ; Surgery ; Three dimensional printing ; Tissue engineering ; Tissue Scaffolds ; Toluidine ; Toluidine blue ; Transforming growth factors ; Transplants & implants</subject><ispartof>PloS one, 2014-06, Vol.9 (6), p.e99410-e99410</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Ye et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, a pearlescent, cartilage-like matrix had formed that penetrated the top layers of the chitosan scaffold forming a 'cap' on the scaffold. Chondrocytic morphology showed typical cells encased in extracellular matrix which stained positively with toluidine blue. Immunohistochemistry demonstrated positive staining for collagen type II and cartilage proteoglycans, as well as collagen type I. Real time PCR analysis showed up-regulation of collagen type II, aggrecan and SOX9 genes when IPFP-ASCs were stimulated by TGFβ3 and BMP6. Thus, IPFP-ASCs can successfully undergo chondrogenesis using TGFβ3 and BMP6 and the cartilage-like tissue that forms on the surface of 3D-printed chitosan scaffold may prove useful as an osteochondral graft.</description><subject>Adipose Tissue - cytology</subject><subject>Aggrecan</subject><subject>Analysis</subject><subject>Arthritis</subject><subject>Biocompatibility</subject><subject>Biology and Life Sciences</subject><subject>Biomedical materials</subject><subject>Bone marrow</subject><subject>Bone morphogenetic protein 6</subject><subject>Bone morphogenetic proteins</subject><subject>Cartilage</subject><subject>Casing (process)</subject><subject>Chitosan</subject><subject>Chondrogenesis</subject><subject>Collagen</subject><subject>Collagen (type I)</subject><subject>Collagen (type II)</subject><subject>Defects</subject><subject>Engineering and Technology</subject><subject>Ethics</subject><subject>Extracellular matrix</subject><subject>Gene expression</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Joint surgery</subject><subject>Knee</subject><subject>Medicine and Health Sciences</subject><subject>Neurosciences</subject><subject>Patella - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ye, Ken</au><au>Felimban, Raed</au><au>Traianedes, Kathy</au><au>Moulton, Simon E</au><au>Wallace, Gordon G</au><au>Chung, Johnson</au><au>Quigley, Anita</au><au>Choong, Peter F M</au><au>Myers, Damian E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-06-11</date><risdate>2014</risdate><volume>9</volume><issue>6</issue><spage>e99410</spage><epage>e99410</epage><pages>e99410-e99410</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, a pearlescent, cartilage-like matrix had formed that penetrated the top layers of the chitosan scaffold forming a 'cap' on the scaffold. Chondrocytic morphology showed typical cells encased in extracellular matrix which stained positively with toluidine blue. Immunohistochemistry demonstrated positive staining for collagen type II and cartilage proteoglycans, as well as collagen type I. Real time PCR analysis showed up-regulation of collagen type II, aggrecan and SOX9 genes when IPFP-ASCs were stimulated by TGFβ3 and BMP6. Thus, IPFP-ASCs can successfully undergo chondrogenesis using TGFβ3 and BMP6 and the cartilage-like tissue that forms on the surface of 3D-printed chitosan scaffold may prove useful as an osteochondral graft.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24918443</pmid><doi>10.1371/journal.pone.0099410</doi><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adipose Tissue - cytology Aggrecan Analysis Arthritis Biocompatibility Biology and Life Sciences Biomedical materials Bone marrow Bone morphogenetic protein 6 Bone morphogenetic proteins Cartilage Casing (process) Chitosan Chondrogenesis Collagen Collagen (type I) Collagen (type II) Defects Engineering and Technology Ethics Extracellular matrix Gene expression Growth factors Humans Immunohistochemistry Joint surgery Knee Medicine and Health Sciences Neurosciences Patella - cytology Physical Sciences Polymers Proteoglycans Real-Time Polymerase Chain Reaction Scaffolds Sox9 protein Stem cells Stem Cells - cytology Surgery Three dimensional printing Tissue engineering Tissue Scaffolds Toluidine Toluidine blue Transforming growth factors Transplants & implants |
| title | Chondrogenesis of infrapatellar fat pad derived adipose stem cells in 3D printed chitosan scaffold |
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