Correlation between serum tryptase, mast cells positive to tryptase and microvascular density in colo-rectal cancer patients: possible biological-clinical significance
Tryptase is a serin protease stored and released from mast cells (MCs) that plays a role in tumour angiogenesis. In this study we aimed to evaluate serum tryptase levels in colo-rectal cancer (CRC) patients before (STLBS) and after (STLAS) radical surgical resection. We also evaluated mast cell dens...
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| Veröffentlicht in: | PloS one 2014-06, Vol.9 (6), p.e99512-e99512 |
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| creator | Ammendola, Michele Sacco, Rosario Sammarco, Giuseppe Donato, Giuseppe Montemurro, Severino Ruggieri, Eustachio Patruno, Rosa Marech, Ilaria Cariello, Marica Vacca, Angelo Gadaleta, Cosmo Damiano Ranieri, Girolamo |
| description | Tryptase is a serin protease stored and released from mast cells (MCs) that plays a role in tumour angiogenesis. In this study we aimed to evaluate serum tryptase levels in colo-rectal cancer (CRC) patients before (STLBS) and after (STLAS) radical surgical resection. We also evaluated mast cell density positive to tryptase (MCDPT) and microvascular density (MVD) in primary tumour tissue.
A series of 61 patients with stage B and C CRC (according to the Astler and Coller staging system) were selected. Serum blood samples were collected from patients one day before and one day after surgery. Tryptase levels were measured using the UniCAP Tryptase Fluoroenzymeimmunoassay (Pharmacia, Uppsala, Sweden). Tumour sections were immunostained with a primary anti-tryptase antibody (clone AA1; Dako, Glostrup, Denmark) and an anti CD-34 antibody (QB-END 10; Bio-Optica Milan, Italy) by means of immunohistochemistry and then evaluated by image analysis methods.
The mean ± s.d. STLBS and STLAS was 5.63±2.61 µg/L, and 3.39±1.47 µg/L respectively and a significant difference between mean levels was found: p = 0.000 by t-test. The mean ± s.d. of MCDPT and MVD was 8.13±3.28 and 29.16±7.39 respectively. A strong correlation between STLBS and MVD (r = 0.83, p = 0.000); STLBS and MCDPT (r = 0.60, p = 0.003); and MCDPT and MVD (r = 0.73; p = 0.001) was found.
Results demonstrated higher STLBS in CRC patients, indicating an involvement of MC tryptase in CRC angiogenesis. Data also indicated lower STLAS, suggesting the release of tryptase from tumour-infiltrating MCs. Serum tryptase levels may therefore play a role as a novel bio-marker predictive of response to radical surgery. In this context tryptase inhibitors such as Gabexate and Nafamostat Mesilate might be evaluated in adjuvant clinical trials as a new anti-angiogenic approach. |
| doi_str_mv | 10.1371/journal.pone.0099512 |
| format | Article |
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A series of 61 patients with stage B and C CRC (according to the Astler and Coller staging system) were selected. Serum blood samples were collected from patients one day before and one day after surgery. Tryptase levels were measured using the UniCAP Tryptase Fluoroenzymeimmunoassay (Pharmacia, Uppsala, Sweden). Tumour sections were immunostained with a primary anti-tryptase antibody (clone AA1; Dako, Glostrup, Denmark) and an anti CD-34 antibody (QB-END 10; Bio-Optica Milan, Italy) by means of immunohistochemistry and then evaluated by image analysis methods.
The mean ± s.d. STLBS and STLAS was 5.63±2.61 µg/L, and 3.39±1.47 µg/L respectively and a significant difference between mean levels was found: p = 0.000 by t-test. The mean ± s.d. of MCDPT and MVD was 8.13±3.28 and 29.16±7.39 respectively. A strong correlation between STLBS and MVD (r = 0.83, p = 0.000); STLBS and MCDPT (r = 0.60, p = 0.003); and MCDPT and MVD (r = 0.73; p = 0.001) was found.
Results demonstrated higher STLBS in CRC patients, indicating an involvement of MC tryptase in CRC angiogenesis. Data also indicated lower STLAS, suggesting the release of tryptase from tumour-infiltrating MCs. Serum tryptase levels may therefore play a role as a novel bio-marker predictive of response to radical surgery. In this context tryptase inhibitors such as Gabexate and Nafamostat Mesilate might be evaluated in adjuvant clinical trials as a new anti-angiogenic approach.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0099512</identifier><identifier>PMID: 24915568</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Angiogenesis ; Biology and Life Sciences ; Biomarkers ; Cancer ; Care and treatment ; Cell density ; Clinical significance ; Clinical trials ; Colorectal cancer ; Colorectal Neoplasms - blood ; Colorectal Neoplasms - blood supply ; Colorectal Neoplasms - enzymology ; Colorectal Neoplasms - surgery ; Correlation ; Density ; Female ; Hostages ; Humans ; Image analysis ; Image processing ; Immunohistochemistry ; Intestinal Mucosa - pathology ; Male ; Mast cells ; Mast Cells - enzymology ; Mast Cells - pathology ; Medical research ; Medical schools ; Medicine and Health Sciences ; Metastasis ; Microvasculature ; Microvessels - pathology ; Multiple myeloma ; Oncology ; Patients ; Proteases ; Rectum ; Science ; Surgery ; Tryptase ; Tryptases - blood ; Tumors ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - metabolism</subject><ispartof>PloS one, 2014-06, Vol.9 (6), p.e99512-e99512</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Ammendola et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Ammendola et al 2014 Ammendola et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-b556dbf094a158d70a0e0d8c2df5a59cf8e13c4ec6a18570c1f85d09badf8f143</citedby><cites>FETCH-LOGICAL-c692t-b556dbf094a158d70a0e0d8c2df5a59cf8e13c4ec6a18570c1f85d09badf8f143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051753/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4051753/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24915568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ammendola, Michele</creatorcontrib><creatorcontrib>Sacco, Rosario</creatorcontrib><creatorcontrib>Sammarco, Giuseppe</creatorcontrib><creatorcontrib>Donato, Giuseppe</creatorcontrib><creatorcontrib>Montemurro, Severino</creatorcontrib><creatorcontrib>Ruggieri, Eustachio</creatorcontrib><creatorcontrib>Patruno, Rosa</creatorcontrib><creatorcontrib>Marech, Ilaria</creatorcontrib><creatorcontrib>Cariello, Marica</creatorcontrib><creatorcontrib>Vacca, Angelo</creatorcontrib><creatorcontrib>Gadaleta, Cosmo Damiano</creatorcontrib><creatorcontrib>Ranieri, Girolamo</creatorcontrib><title>Correlation between serum tryptase, mast cells positive to tryptase and microvascular density in colo-rectal cancer patients: possible biological-clinical significance</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Tryptase is a serin protease stored and released from mast cells (MCs) that plays a role in tumour angiogenesis. In this study we aimed to evaluate serum tryptase levels in colo-rectal cancer (CRC) patients before (STLBS) and after (STLAS) radical surgical resection. We also evaluated mast cell density positive to tryptase (MCDPT) and microvascular density (MVD) in primary tumour tissue.
A series of 61 patients with stage B and C CRC (according to the Astler and Coller staging system) were selected. Serum blood samples were collected from patients one day before and one day after surgery. Tryptase levels were measured using the UniCAP Tryptase Fluoroenzymeimmunoassay (Pharmacia, Uppsala, Sweden). Tumour sections were immunostained with a primary anti-tryptase antibody (clone AA1; Dako, Glostrup, Denmark) and an anti CD-34 antibody (QB-END 10; Bio-Optica Milan, Italy) by means of immunohistochemistry and then evaluated by image analysis methods.
The mean ± s.d. STLBS and STLAS was 5.63±2.61 µg/L, and 3.39±1.47 µg/L respectively and a significant difference between mean levels was found: p = 0.000 by t-test. The mean ± s.d. of MCDPT and MVD was 8.13±3.28 and 29.16±7.39 respectively. A strong correlation between STLBS and MVD (r = 0.83, p = 0.000); STLBS and MCDPT (r = 0.60, p = 0.003); and MCDPT and MVD (r = 0.73; p = 0.001) was found.
Results demonstrated higher STLBS in CRC patients, indicating an involvement of MC tryptase in CRC angiogenesis. Data also indicated lower STLAS, suggesting the release of tryptase from tumour-infiltrating MCs. Serum tryptase levels may therefore play a role as a novel bio-marker predictive of response to radical surgery. In this context tryptase inhibitors such as Gabexate and Nafamostat Mesilate might be evaluated in adjuvant clinical trials as a new anti-angiogenic approach.</description><subject>Angiogenesis</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Cell density</subject><subject>Clinical significance</subject><subject>Clinical trials</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - blood</subject><subject>Colorectal Neoplasms - blood supply</subject><subject>Colorectal Neoplasms - enzymology</subject><subject>Colorectal Neoplasms - surgery</subject><subject>Correlation</subject><subject>Density</subject><subject>Female</subject><subject>Hostages</subject><subject>Humans</subject><subject>Image analysis</subject><subject>Image processing</subject><subject>Immunohistochemistry</subject><subject>Intestinal Mucosa - pathology</subject><subject>Male</subject><subject>Mast cells</subject><subject>Mast Cells - enzymology</subject><subject>Mast Cells - pathology</subject><subject>Medical research</subject><subject>Medical schools</subject><subject>Medicine and Health Sciences</subject><subject>Metastasis</subject><subject>Microvasculature</subject><subject>Microvessels - pathology</subject><subject>Multiple myeloma</subject><subject>Oncology</subject><subject>Patients</subject><subject>Proteases</subject><subject>Rectum</subject><subject>Science</subject><subject>Surgery</subject><subject>Tryptase</subject><subject>Tryptases - blood</subject><subject>Tumors</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11rFDEUhgdRbK3-A9GAIArumsxMMhkvhFL8KBQKft2GM5kz25RssiaZ6v4i_6aZ3W3pSi8kFwmZ533PnJNziuIpo3NWNeztpR-DAztfeYdzStuWs_JeccjaqpyJklb3b50PikcxXlLKKynEw-KgrFvGuZCHxZ8THwJaSMY70mH6hehIxDAuSQrrVYKIb8gSYiIarY1k5aNJ5gpJ8jcAAdeTpdHBX0HUo4VAenSZWxPjiPbWzwLqBJZocBoDWeVw6FJ8N9lF01kkncnYwmiwM22Nmw4kmoUzg9mIHhcPBrARn-z2o-L7xw_fTj7Pzs4_nZ4cn820aMs063JWfTfQtgbGZd9QoEh7qct-4MBbPUhkla5RC2CSN1SzQfKeth30gxxYXR0Vz7e-K-uj2tU4KsarmpdU0ok43RK9h0u1CmYJYa08GLW58GGhICSjLaq6pVDTRlDB-pqyoZVYStGXQrJGi67NXu930cZuib3ORQlg90z3vzhzoRb-StWUs4ZX2eDVziD4nyPGpJYmTi8FDv24-W8uStE2U6wX_6B3Z7ejFpATMG7wOa6eTNVxzaSouJQsU_M7qLx6zH2QG3Iw-X5P8HpPkJmEv9MCxhjV6dcv_8-e_9hnX95iLxBsuojejlM7x32w3oK5S2MMONwUmVE1zdN1NdQ0T2o3T1n27PYD3YiuB6j6Cxi9Hw0</recordid><startdate>20140610</startdate><enddate>20140610</enddate><creator>Ammendola, Michele</creator><creator>Sacco, Rosario</creator><creator>Sammarco, Giuseppe</creator><creator>Donato, Giuseppe</creator><creator>Montemurro, Severino</creator><creator>Ruggieri, Eustachio</creator><creator>Patruno, Rosa</creator><creator>Marech, Ilaria</creator><creator>Cariello, Marica</creator><creator>Vacca, Angelo</creator><creator>Gadaleta, Cosmo Damiano</creator><creator>Ranieri, Girolamo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140610</creationdate><title>Correlation between serum tryptase, mast cells positive to tryptase and microvascular density in colo-rectal cancer patients: possible biological-clinical significance</title><author>Ammendola, Michele ; Sacco, Rosario ; Sammarco, Giuseppe ; Donato, Giuseppe ; Montemurro, Severino ; Ruggieri, Eustachio ; Patruno, Rosa ; Marech, Ilaria ; Cariello, Marica ; Vacca, Angelo ; Gadaleta, Cosmo Damiano ; Ranieri, Girolamo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-b556dbf094a158d70a0e0d8c2df5a59cf8e13c4ec6a18570c1f85d09badf8f143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Angiogenesis</topic><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>Cell density</topic><topic>Clinical significance</topic><topic>Clinical trials</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ammendola, Michele</au><au>Sacco, Rosario</au><au>Sammarco, Giuseppe</au><au>Donato, Giuseppe</au><au>Montemurro, Severino</au><au>Ruggieri, Eustachio</au><au>Patruno, Rosa</au><au>Marech, Ilaria</au><au>Cariello, Marica</au><au>Vacca, Angelo</au><au>Gadaleta, Cosmo Damiano</au><au>Ranieri, Girolamo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation between serum tryptase, mast cells positive to tryptase and microvascular density in colo-rectal cancer patients: possible biological-clinical significance</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-06-10</date><risdate>2014</risdate><volume>9</volume><issue>6</issue><spage>e99512</spage><epage>e99512</epage><pages>e99512-e99512</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Tryptase is a serin protease stored and released from mast cells (MCs) that plays a role in tumour angiogenesis. In this study we aimed to evaluate serum tryptase levels in colo-rectal cancer (CRC) patients before (STLBS) and after (STLAS) radical surgical resection. We also evaluated mast cell density positive to tryptase (MCDPT) and microvascular density (MVD) in primary tumour tissue.
A series of 61 patients with stage B and C CRC (according to the Astler and Coller staging system) were selected. Serum blood samples were collected from patients one day before and one day after surgery. Tryptase levels were measured using the UniCAP Tryptase Fluoroenzymeimmunoassay (Pharmacia, Uppsala, Sweden). Tumour sections were immunostained with a primary anti-tryptase antibody (clone AA1; Dako, Glostrup, Denmark) and an anti CD-34 antibody (QB-END 10; Bio-Optica Milan, Italy) by means of immunohistochemistry and then evaluated by image analysis methods.
The mean ± s.d. STLBS and STLAS was 5.63±2.61 µg/L, and 3.39±1.47 µg/L respectively and a significant difference between mean levels was found: p = 0.000 by t-test. The mean ± s.d. of MCDPT and MVD was 8.13±3.28 and 29.16±7.39 respectively. A strong correlation between STLBS and MVD (r = 0.83, p = 0.000); STLBS and MCDPT (r = 0.60, p = 0.003); and MCDPT and MVD (r = 0.73; p = 0.001) was found.
Results demonstrated higher STLBS in CRC patients, indicating an involvement of MC tryptase in CRC angiogenesis. Data also indicated lower STLAS, suggesting the release of tryptase from tumour-infiltrating MCs. Serum tryptase levels may therefore play a role as a novel bio-marker predictive of response to radical surgery. In this context tryptase inhibitors such as Gabexate and Nafamostat Mesilate might be evaluated in adjuvant clinical trials as a new anti-angiogenic approach.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24915568</pmid><doi>10.1371/journal.pone.0099512</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-06, Vol.9 (6), p.e99512-e99512 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1534520804 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Angiogenesis Biology and Life Sciences Biomarkers Cancer Care and treatment Cell density Clinical significance Clinical trials Colorectal cancer Colorectal Neoplasms - blood Colorectal Neoplasms - blood supply Colorectal Neoplasms - enzymology Colorectal Neoplasms - surgery Correlation Density Female Hostages Humans Image analysis Image processing Immunohistochemistry Intestinal Mucosa - pathology Male Mast cells Mast Cells - enzymology Mast Cells - pathology Medical research Medical schools Medicine and Health Sciences Metastasis Microvasculature Microvessels - pathology Multiple myeloma Oncology Patients Proteases Rectum Science Surgery Tryptase Tryptases - blood Tumors Vascular endothelial growth factor Vascular Endothelial Growth Factor A - metabolism |
| title | Correlation between serum tryptase, mast cells positive to tryptase and microvascular density in colo-rectal cancer patients: possible biological-clinical significance |
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