A PCR based protocol for detecting indel mutations induced by TALENs and CRISPR/Cas9 in zebrafish

Genome editing techniques such as the zinc-finger nucleases (ZFNs), transcription activator-like effecter nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system Cas9 can induce efficient DNA double strand breaks (DSBs) at the target g...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2014-06, Vol.9 (6), p.e98282
Hauptverfasser:	Yu, Chuan, Zhang, Yaguang, Yao, Shaohua, Wei, Yuquan
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animal care Animals Assaying Base Sequence Biology and life sciences Cancer CRISPR CRISPR-Cas Systems Danio rerio Deoxyribonucleic acid Design DNA DNA damage DNA Mutational Analysis DNA repair DNA sequencing Editing Endonuclease Enzymes Female Gene loci Gene mutation Gene sequencing Genetic aspects Genetic engineering Genetic Loci Genetic polymorphisms Genetic research Genome editing Genomes Genomics Homology INDEL Mutation Laboratories Life sciences Male Melting curve Molecular Sequence Data Mutagenesis Mutation Non-homologous end joining Nuclease Nucleotide sequence Polymerase Chain Reaction Polymorphism Primers Sequence Alignment Transcription Transcription activator-like effector nucleases Zebrafish Zebrafish - genetics Zinc Zinc finger proteins
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!