A versatile system for USER cloning-based assembly of expression vectors for mammalian cell engineering

A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique--USER cloning--to rapidly construct mammalian expression vectors of multiple DNA fragments an...

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Veröffentlicht in:	PloS one 2014-05, Vol.9 (5), p.e96693-e96693
Hauptverfasser:	Lund, Anne Mathilde, Kildegaard, Helene Faustrup, Petersen, Maja Borup Kjær, Rank, Julie, Hansen, Bjarne Gram, Andersen, Mikael Rørdam, Mortensen, Uffe Hasbro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigenic determinants Assembly Biology Biology and Life Sciences Biomarkers - metabolism Biotechnology Cell Engineering - methods Cell lines Cells (Biology) CHO Cells Cloning Cloning vectors Cloning, Molecular - methods Construction Cricetinae Cricetulus Deoxyribonucleic acid Dihydrofolate reductase DNA DNA - genetics E coli Engineering Enzymes Epitopes Expression vectors Fluorescence Fragments Gene Expression Gene sequencing Genetic engineering Genetic research Genetic vectors Genetic Vectors - genetics Genomes Genomics Green Fluorescent Proteins - secretion HEK293 Cells Humans Internal ribosome entry site Localization Mammalian cells Mammals Plasmids Protein purification Proteins Purification Pyrimidines Tags Uracil Vectors (Biology)
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