Purification and characterization of an extracellular, thermo-alkali-stable, metal tolerant laccase from Bacillus tequilensis SN4

A novel extracellular thermo-alkali-stable laccase from Bacillus tequilensis SN4 (SN4LAC) was purified to homogeneity. The laccase was a monomeric protein of molecular weight 32 KDa. UV-visible spectrum and peptide mass fingerprinting results showed that SN4LAC is a multicopper oxidase. Laccase was...

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Veröffentlicht in:	PloS one 2014-05, Vol.9 (5), p.e96951
Hauptverfasser:	Sondhi, Sonica, Sharma, Prince, Saini, Shilpa, Puri, Neena, Gupta, Naveen
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkali metals Analysis of Variance Bacillus Bacillus - enzymology Bacillus subtilis Bacillus tequilensis Biology and Life Sciences Carbon dioxide Catalysis Cellulose Chemical properties Cobalt Copper Cysteine Cysteine - pharmacology Detergents Dithiothreitol Dithiothreitol - pharmacology Dyes Electrophoresis, Polyacrylamide Gel Enzyme Stability - physiology Enzymes Extreme high temperatures Fingerprinting Guaiacol Halides Heavy metals High temperature Homogeneity Hydrogen ions Hydrogen-Ion Concentration Industrial applications Kinetics Laccase Laccase - antagonists & inhibitors Laccase - isolation & purification Mercaptoethanol - pharmacology Metal ions Molecular weight Multicopper oxidase Oxidases Oxidation Oxidoreductases - antagonists & inhibitors Oxidoreductases - isolation & purification Peptide mapping pH effects Phenolic compounds Phenols Pleurotus ostreatus Pollutants Polyethylene glycol Proteins Pulp & paper mills Pyrogallol - analogs & derivatives Pyrogallol - metabolism Sodium Sodium azide Sodium Azide - pharmacology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Substrate Specificity Substrates Surface active agents Surfactants Syringaldazine Temperature Visible spectrum Water treatment
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