Transcriptional response of virus-infected cassava and identification of putative sources of resistance for cassava brown streak disease
Cassava (Manihot esculenta) is a major food staple in sub-Saharan Africa, which is severely affected by cassava brown streak disease (CBSD). The aim of this study was to identify resistance for CBSD as well as to understand the mechanism of putative resistance for providing effective control for the...
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| description | Cassava (Manihot esculenta) is a major food staple in sub-Saharan Africa, which is severely affected by cassava brown streak disease (CBSD). The aim of this study was to identify resistance for CBSD as well as to understand the mechanism of putative resistance for providing effective control for the disease. Three cassava varieties; Kaleso, Kiroba and Albert were inoculated with cassava brown streak viruses by grafting and also using the natural insect vector the whitefly, Bemisia tabaci. Kaleso expressed mild or no disease symptoms and supported low concentrations of viruses, which is a characteristic of resistant plants. In comparison, Kiroba expressed severe leaf but milder root symptoms, while Albert was susceptible with severe symptoms both on leaves and roots. Real-time PCR was used to estimate virus concentrations in cassava varieties. Virus quantities were higher in Kiroba and Albert compared to Kaleso. The Illumina RNA-sequencing was used to further understand the genetic basis of resistance. More than 700 genes were uniquely overexpressed in Kaleso in response to virus infection compared to Albert. Surprisingly, none of them were similar to known resistant gene orthologs. Some of the overexpressed genes, however, belonged to the hormone signalling pathways and secondary metabolites, both of which are linked to plant resistance. These genes should be further characterised before confirming their role in resistance to CBSD. |
| doi_str_mv | 10.1371/journal.pone.0096642 |
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The aim of this study was to identify resistance for CBSD as well as to understand the mechanism of putative resistance for providing effective control for the disease. Three cassava varieties; Kaleso, Kiroba and Albert were inoculated with cassava brown streak viruses by grafting and also using the natural insect vector the whitefly, Bemisia tabaci. Kaleso expressed mild or no disease symptoms and supported low concentrations of viruses, which is a characteristic of resistant plants. In comparison, Kiroba expressed severe leaf but milder root symptoms, while Albert was susceptible with severe symptoms both on leaves and roots. Real-time PCR was used to estimate virus concentrations in cassava varieties. Virus quantities were higher in Kiroba and Albert compared to Kaleso. The Illumina RNA-sequencing was used to further understand the genetic basis of resistance. More than 700 genes were uniquely overexpressed in Kaleso in response to virus infection compared to Albert. Surprisingly, none of them were similar to known resistant gene orthologs. Some of the overexpressed genes, however, belonged to the hormone signalling pathways and secondary metabolites, both of which are linked to plant resistance. These genes should be further characterised before confirming their role in resistance to CBSD.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0096642</identifier><identifier>PMID: 24846209</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Bemisia tabaci ; Bioinformatics ; Biology and Life Sciences ; Cassava ; Disease ; Disease control ; Disease resistance ; Disease Resistance - genetics ; Food ; Gangrene ; Gene expression ; Gene sequencing ; Genes ; Genetic aspects ; Genomes ; Health aspects ; Infections ; Insects ; Leaves ; Low concentrations ; Manihot - genetics ; Manihot - virology ; Manihot esculenta ; Metabolites ; Natural resources ; Plant Diseases - genetics ; Plant Diseases - virology ; Plant pathology ; Plant resistance ; Plant sciences ; Plant viruses ; Potyviridae - genetics ; Potyviridae - metabolism ; Proteins ; Ribonucleic acid ; RNA ; Secondary metabolites ; Signal transduction ; Signaling ; Transcription ; Transcription (Genetics) ; Trends ; Virus diseases ; Viruses ; Whiteflies</subject><ispartof>PloS one, 2014-05, Vol.9 (5), p.e96642-e96642</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Maruthi et al. 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The aim of this study was to identify resistance for CBSD as well as to understand the mechanism of putative resistance for providing effective control for the disease. Three cassava varieties; Kaleso, Kiroba and Albert were inoculated with cassava brown streak viruses by grafting and also using the natural insect vector the whitefly, Bemisia tabaci. Kaleso expressed mild or no disease symptoms and supported low concentrations of viruses, which is a characteristic of resistant plants. In comparison, Kiroba expressed severe leaf but milder root symptoms, while Albert was susceptible with severe symptoms both on leaves and roots. Real-time PCR was used to estimate virus concentrations in cassava varieties. Virus quantities were higher in Kiroba and Albert compared to Kaleso. The Illumina RNA-sequencing was used to further understand the genetic basis of resistance. More than 700 genes were uniquely overexpressed in Kaleso in response to virus infection compared to Albert. 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These genes should be further characterised before confirming their role in resistance to CBSD.</description><subject>Analysis</subject><subject>Bemisia tabaci</subject><subject>Bioinformatics</subject><subject>Biology and Life Sciences</subject><subject>Cassava</subject><subject>Disease</subject><subject>Disease control</subject><subject>Disease resistance</subject><subject>Disease Resistance - genetics</subject><subject>Food</subject><subject>Gangrene</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>Infections</subject><subject>Insects</subject><subject>Leaves</subject><subject>Low concentrations</subject><subject>Manihot - genetics</subject><subject>Manihot - virology</subject><subject>Manihot esculenta</subject><subject>Metabolites</subject><subject>Natural resources</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maruthi, M N</au><au>Bouvaine, Sophie</au><au>Tufan, Hale A</au><au>Mohammed, Ibrahim U</au><au>Hillocks, Rory J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional response of virus-infected cassava and identification of putative sources of resistance for cassava brown streak disease</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-05-20</date><risdate>2014</risdate><volume>9</volume><issue>5</issue><spage>e96642</spage><epage>e96642</epage><pages>e96642-e96642</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Cassava (Manihot esculenta) is a major food staple in sub-Saharan Africa, which is severely affected by cassava brown streak disease (CBSD). The aim of this study was to identify resistance for CBSD as well as to understand the mechanism of putative resistance for providing effective control for the disease. Three cassava varieties; Kaleso, Kiroba and Albert were inoculated with cassava brown streak viruses by grafting and also using the natural insect vector the whitefly, Bemisia tabaci. Kaleso expressed mild or no disease symptoms and supported low concentrations of viruses, which is a characteristic of resistant plants. In comparison, Kiroba expressed severe leaf but milder root symptoms, while Albert was susceptible with severe symptoms both on leaves and roots. Real-time PCR was used to estimate virus concentrations in cassava varieties. Virus quantities were higher in Kiroba and Albert compared to Kaleso. The Illumina RNA-sequencing was used to further understand the genetic basis of resistance. More than 700 genes were uniquely overexpressed in Kaleso in response to virus infection compared to Albert. Surprisingly, none of them were similar to known resistant gene orthologs. Some of the overexpressed genes, however, belonged to the hormone signalling pathways and secondary metabolites, both of which are linked to plant resistance. These genes should be further characterised before confirming their role in resistance to CBSD.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24846209</pmid><doi>10.1371/journal.pone.0096642</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Bemisia tabaci Bioinformatics Biology and Life Sciences Cassava Disease Disease control Disease resistance Disease Resistance - genetics Food Gangrene Gene expression Gene sequencing Genes Genetic aspects Genomes Health aspects Infections Insects Leaves Low concentrations Manihot - genetics Manihot - virology Manihot esculenta Metabolites Natural resources Plant Diseases - genetics Plant Diseases - virology Plant pathology Plant resistance Plant sciences Plant viruses Potyviridae - genetics Potyviridae - metabolism Proteins Ribonucleic acid RNA Secondary metabolites Signal transduction Signaling Transcription Transcription (Genetics) Trends Virus diseases Viruses Whiteflies |
| title | Transcriptional response of virus-infected cassava and identification of putative sources of resistance for cassava brown streak disease |
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