Evaluation of global differential gene and protein expression in primary Pterygium: S100A8 and S100A9 as possible drivers of a signaling network
Pterygium is a wing shaped fibrovascular growth on the ocular surface, characterized by fibrosis, angiogenesis, extracellular matrix remodeling, and inflammatory infiltrates. Epidemiologic studies have linked pterygium formation to various chronic inflammatory conditions, such as ultraviolet radiati...
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| creator | Hou, Aihua Lan, Wanwen Law, Kai Pong Khoo, Ser Chin Jasmine Tin, Min Qi Lim, Yoon Pin Tong, Louis |
| description | Pterygium is a wing shaped fibrovascular growth on the ocular surface, characterized by fibrosis, angiogenesis, extracellular matrix remodeling, and inflammatory infiltrates. Epidemiologic studies have linked pterygium formation to various chronic inflammatory conditions, such as ultraviolet radiation, sawdust exposure, and dry eye disease. The purpose of this study is to identify proteins that are differentially expressed in primary pterygium by using a combination of gene microarray and proteomic platforms.
Paired pterygium and uninvolved conjunctiva tissues of four patients were evaluated for differences in global gene transcript levels using a genechip microarray. Proteins extracted from another four pairs of tissues were quantified by iTRAQ approach. Western blot and immunofluorescent staining on additional patients were used to validate dysregulated protein expression obtained from microarray and proteomics data. In addition, primary conjunctival fibroblasts were treated with recombinant S100A8, S100A9 or both. Transcript level changes of a panel of potential target genes were evaluated by real time-PCR.
The following were up-regulated at both protein and transcript levels S100 A8 and A9, aldehyde dehydrogenase 3 family, member1 (ALDH3A1) and vimentin (VIM). Conversely, serpin peptidase inhibitor clade A member 1 (SERPINA1) and transferrin (TF) were down-regulated. Upon adding S100A8, S100A9 or both, the inflammatory chemokine CXCL1, matrix proteins vimentin, biglycan, and gelsolin, as well as annexin-A2, thymosin-β4, chymase (CMA1), member of Ras oncogene family RAB10 and SERPINA1 were found to be up-regulated.
We identified 3 up-regulated and 2 down-regulated proteins by using a stringent approach comparing microarray and proteomic data. On stimulating cells with S100A8/9, a repertoire of key genes found to be up-regulated in pterygium tissue, were induced in these cells. S100A8/9 may be an upstream trigger for inflammation and other disease pathways in pterygium. |
| doi_str_mv | 10.1371/journal.pone.0097402 |
| format | Article |
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Paired pterygium and uninvolved conjunctiva tissues of four patients were evaluated for differences in global gene transcript levels using a genechip microarray. Proteins extracted from another four pairs of tissues were quantified by iTRAQ approach. Western blot and immunofluorescent staining on additional patients were used to validate dysregulated protein expression obtained from microarray and proteomics data. In addition, primary conjunctival fibroblasts were treated with recombinant S100A8, S100A9 or both. Transcript level changes of a panel of potential target genes were evaluated by real time-PCR.
The following were up-regulated at both protein and transcript levels S100 A8 and A9, aldehyde dehydrogenase 3 family, member1 (ALDH3A1) and vimentin (VIM). Conversely, serpin peptidase inhibitor clade A member 1 (SERPINA1) and transferrin (TF) were down-regulated. Upon adding S100A8, S100A9 or both, the inflammatory chemokine CXCL1, matrix proteins vimentin, biglycan, and gelsolin, as well as annexin-A2, thymosin-β4, chymase (CMA1), member of Ras oncogene family RAB10 and SERPINA1 were found to be up-regulated.
We identified 3 up-regulated and 2 down-regulated proteins by using a stringent approach comparing microarray and proteomic data. On stimulating cells with S100A8/9, a repertoire of key genes found to be up-regulated in pterygium tissue, were induced in these cells. S100A8/9 may be an upstream trigger for inflammation and other disease pathways in pterygium.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0097402</identifier><identifier>PMID: 24825356</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aldehyde dehydrogenase ; Aldehyde Dehydrogenase - metabolism ; alpha 1-Antitrypsin - metabolism ; Angiogenesis ; Biglycan - metabolism ; Biochemistry ; Biology and Life Sciences ; Blotting, Western ; Calgranulin A - genetics ; Calgranulin A - metabolism ; Calgranulin B - genetics ; Calgranulin B - metabolism ; Chemokine CXCL1 - metabolism ; Chymase ; Conjunctiva ; Cornea ; DNA microarrays ; Epidemiology ; Extracellular matrix ; Fibroblasts ; Fibrosis ; Fluorescent Antibody Technique ; Gelsolin ; Gelsolin - metabolism ; Gene expression ; Gene Expression Regulation - genetics ; Genes ; Genomes ; Genomics ; Humans ; Inflammation ; Medicine and Health Sciences ; Oligonucleotide Array Sequence Analysis ; Pathogenesis ; Patients ; Peptidase ; Protein arrays ; Proteins ; Proteomics ; Proteomics - methods ; Pterygium - genetics ; Pterygium - metabolism ; Real-Time Polymerase Chain Reaction ; RNA polymerase ; Sawdust ; Signal Transduction - genetics ; Signaling ; Tissues ; Transcription ; Transferrin ; Transferrin - metabolism ; U.V. radiation ; Ultraviolet radiation ; Vimentin ; Vimentin - metabolism</subject><ispartof>PloS one, 2014-05, Vol.9 (5), p.e97402-e97402</ispartof><rights>2014 Hou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Hou et al 2014 Hou et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-479445753e779ed8594f907a84a1a8b32e5c90520cf8afdf18386083907b90e13</citedby><cites>FETCH-LOGICAL-c526t-479445753e779ed8594f907a84a1a8b32e5c90520cf8afdf18386083907b90e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019582/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4019582/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24825356$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Coleman, William B.</contributor><creatorcontrib>Hou, Aihua</creatorcontrib><creatorcontrib>Lan, Wanwen</creatorcontrib><creatorcontrib>Law, Kai Pong</creatorcontrib><creatorcontrib>Khoo, Ser Chin Jasmine</creatorcontrib><creatorcontrib>Tin, Min Qi</creatorcontrib><creatorcontrib>Lim, Yoon Pin</creatorcontrib><creatorcontrib>Tong, Louis</creatorcontrib><title>Evaluation of global differential gene and protein expression in primary Pterygium: S100A8 and S100A9 as possible drivers of a signaling network</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Pterygium is a wing shaped fibrovascular growth on the ocular surface, characterized by fibrosis, angiogenesis, extracellular matrix remodeling, and inflammatory infiltrates. Epidemiologic studies have linked pterygium formation to various chronic inflammatory conditions, such as ultraviolet radiation, sawdust exposure, and dry eye disease. The purpose of this study is to identify proteins that are differentially expressed in primary pterygium by using a combination of gene microarray and proteomic platforms.
Paired pterygium and uninvolved conjunctiva tissues of four patients were evaluated for differences in global gene transcript levels using a genechip microarray. Proteins extracted from another four pairs of tissues were quantified by iTRAQ approach. Western blot and immunofluorescent staining on additional patients were used to validate dysregulated protein expression obtained from microarray and proteomics data. In addition, primary conjunctival fibroblasts were treated with recombinant S100A8, S100A9 or both. Transcript level changes of a panel of potential target genes were evaluated by real time-PCR.
The following were up-regulated at both protein and transcript levels S100 A8 and A9, aldehyde dehydrogenase 3 family, member1 (ALDH3A1) and vimentin (VIM). Conversely, serpin peptidase inhibitor clade A member 1 (SERPINA1) and transferrin (TF) were down-regulated. Upon adding S100A8, S100A9 or both, the inflammatory chemokine CXCL1, matrix proteins vimentin, biglycan, and gelsolin, as well as annexin-A2, thymosin-β4, chymase (CMA1), member of Ras oncogene family RAB10 and SERPINA1 were found to be up-regulated.
We identified 3 up-regulated and 2 down-regulated proteins by using a stringent approach comparing microarray and proteomic data. On stimulating cells with S100A8/9, a repertoire of key genes found to be up-regulated in pterygium tissue, were induced in these cells. S100A8/9 may be an upstream trigger for inflammation and other disease pathways in pterygium.</description><subject>Aldehyde dehydrogenase</subject><subject>Aldehyde Dehydrogenase - metabolism</subject><subject>alpha 1-Antitrypsin - metabolism</subject><subject>Angiogenesis</subject><subject>Biglycan - metabolism</subject><subject>Biochemistry</subject><subject>Biology and Life Sciences</subject><subject>Blotting, Western</subject><subject>Calgranulin A - genetics</subject><subject>Calgranulin A - metabolism</subject><subject>Calgranulin B - genetics</subject><subject>Calgranulin B - metabolism</subject><subject>Chemokine CXCL1 - metabolism</subject><subject>Chymase</subject><subject>Conjunctiva</subject><subject>Cornea</subject><subject>DNA microarrays</subject><subject>Epidemiology</subject><subject>Extracellular matrix</subject><subject>Fibroblasts</subject><subject>Fibrosis</subject><subject>Fluorescent Antibody Technique</subject><subject>Gelsolin</subject><subject>Gelsolin - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - genetics</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Medicine and Health Sciences</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Peptidase</subject><subject>Protein arrays</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>Pterygium - genetics</subject><subject>Pterygium - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA polymerase</subject><subject>Sawdust</subject><subject>Signal Transduction - genetics</subject><subject>Signaling</subject><subject>Tissues</subject><subject>Transcription</subject><subject>Transferrin</subject><subject>Transferrin - metabolism</subject><subject>U.V. radiation</subject><subject>Ultraviolet radiation</subject><subject>Vimentin</subject><subject>Vimentin - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1v1DAQjRCIlsI_QGCJC5dd_JnYHJCqqkClSiABZ8tJxsGL1w52Uui_4Cfj3U2rFnHyjP3em3njqarnBK8Ja8ibTZxTMH49xgBrjFXDMX1QHRPF6KqmmD28Ex9VT3LeYCyYrOvH1RHlkgom6uPqz_mV8bOZXAwoWjT42BqPemctJAiTK8kAAZAJPRpTnMAFBL_HBDnvKCUbk9uadI0-T5CuBzdv36IvBONTuefsQ4VMRmMslNYD6pO7gpR35QzKbigmXBhQgOlXTD-eVo-s8RmeLedJ9e39-dezj6vLTx8uzk4vV52g9bTijeJcNIJB0yjopVDcKtwYyQ0xsmUURKewoLiz0tjeElmsY8kKplUYCDupXh50Rx-zXoaZNRGUUyqIEgVxcUD00Wz0YlNH4_T-IqZBmzS5zoNmxCpghLcW97ynXHWq27XEWEtLzb5ovVuqze0W-q6MNhl_T_T-S3Df9RCvNMelFUmLwOtFIMWfM-RJb13uwHsTIM6HviWpBWMF-uof6P_d8QOqS-VjEtjbZgjWuwW7YendgullwQrtxV0jt6SbjWJ_AeAhzjI</recordid><startdate>20140513</startdate><enddate>20140513</enddate><creator>Hou, Aihua</creator><creator>Lan, Wanwen</creator><creator>Law, Kai Pong</creator><creator>Khoo, Ser Chin Jasmine</creator><creator>Tin, Min Qi</creator><creator>Lim, Yoon Pin</creator><creator>Tong, Louis</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140513</creationdate><title>Evaluation of global differential gene and protein expression in primary Pterygium: S100A8 and S100A9 as possible drivers of a signaling network</title><author>Hou, Aihua ; Lan, Wanwen ; Law, Kai Pong ; Khoo, Ser Chin Jasmine ; Tin, Min Qi ; Lim, Yoon Pin ; Tong, Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-479445753e779ed8594f907a84a1a8b32e5c90520cf8afdf18386083907b90e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aldehyde dehydrogenase</topic><topic>Aldehyde Dehydrogenase - metabolism</topic><topic>alpha 1-Antitrypsin - metabolism</topic><topic>Angiogenesis</topic><topic>Biglycan - metabolism</topic><topic>Biochemistry</topic><topic>Biology and Life Sciences</topic><topic>Blotting, Western</topic><topic>Calgranulin A - genetics</topic><topic>Calgranulin A - metabolism</topic><topic>Calgranulin B - genetics</topic><topic>Calgranulin B - metabolism</topic><topic>Chemokine CXCL1 - metabolism</topic><topic>Chymase</topic><topic>Conjunctiva</topic><topic>Cornea</topic><topic>DNA microarrays</topic><topic>Epidemiology</topic><topic>Extracellular matrix</topic><topic>Fibroblasts</topic><topic>Fibrosis</topic><topic>Fluorescent Antibody Technique</topic><topic>Gelsolin</topic><topic>Gelsolin - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - genetics</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Medicine and Health Sciences</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Pathogenesis</topic><topic>Patients</topic><topic>Peptidase</topic><topic>Protein arrays</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><topic>Pterygium - genetics</topic><topic>Pterygium - metabolism</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA polymerase</topic><topic>Sawdust</topic><topic>Signal Transduction - genetics</topic><topic>Signaling</topic><topic>Tissues</topic><topic>Transcription</topic><topic>Transferrin</topic><topic>Transferrin - metabolism</topic><topic>U.V. radiation</topic><topic>Ultraviolet radiation</topic><topic>Vimentin</topic><topic>Vimentin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hou, Aihua</creatorcontrib><creatorcontrib>Lan, Wanwen</creatorcontrib><creatorcontrib>Law, Kai Pong</creatorcontrib><creatorcontrib>Khoo, Ser Chin Jasmine</creatorcontrib><creatorcontrib>Tin, Min Qi</creatorcontrib><creatorcontrib>Lim, Yoon Pin</creatorcontrib><creatorcontrib>Tong, Louis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database (ProQuest)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection (Proquest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hou, Aihua</au><au>Lan, Wanwen</au><au>Law, Kai Pong</au><au>Khoo, Ser Chin Jasmine</au><au>Tin, Min Qi</au><au>Lim, Yoon Pin</au><au>Tong, Louis</au><au>Coleman, William B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of global differential gene and protein expression in primary Pterygium: S100A8 and S100A9 as possible drivers of a signaling network</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-05-13</date><risdate>2014</risdate><volume>9</volume><issue>5</issue><spage>e97402</spage><epage>e97402</epage><pages>e97402-e97402</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Pterygium is a wing shaped fibrovascular growth on the ocular surface, characterized by fibrosis, angiogenesis, extracellular matrix remodeling, and inflammatory infiltrates. Epidemiologic studies have linked pterygium formation to various chronic inflammatory conditions, such as ultraviolet radiation, sawdust exposure, and dry eye disease. The purpose of this study is to identify proteins that are differentially expressed in primary pterygium by using a combination of gene microarray and proteomic platforms.
Paired pterygium and uninvolved conjunctiva tissues of four patients were evaluated for differences in global gene transcript levels using a genechip microarray. Proteins extracted from another four pairs of tissues were quantified by iTRAQ approach. Western blot and immunofluorescent staining on additional patients were used to validate dysregulated protein expression obtained from microarray and proteomics data. In addition, primary conjunctival fibroblasts were treated with recombinant S100A8, S100A9 or both. Transcript level changes of a panel of potential target genes were evaluated by real time-PCR.
The following were up-regulated at both protein and transcript levels S100 A8 and A9, aldehyde dehydrogenase 3 family, member1 (ALDH3A1) and vimentin (VIM). Conversely, serpin peptidase inhibitor clade A member 1 (SERPINA1) and transferrin (TF) were down-regulated. Upon adding S100A8, S100A9 or both, the inflammatory chemokine CXCL1, matrix proteins vimentin, biglycan, and gelsolin, as well as annexin-A2, thymosin-β4, chymase (CMA1), member of Ras oncogene family RAB10 and SERPINA1 were found to be up-regulated.
We identified 3 up-regulated and 2 down-regulated proteins by using a stringent approach comparing microarray and proteomic data. On stimulating cells with S100A8/9, a repertoire of key genes found to be up-regulated in pterygium tissue, were induced in these cells. S100A8/9 may be an upstream trigger for inflammation and other disease pathways in pterygium.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24825356</pmid><doi>10.1371/journal.pone.0097402</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1524225195 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; PubMed Central; EZB Electronic Journals Library |
| subjects | Aldehyde dehydrogenase Aldehyde Dehydrogenase - metabolism alpha 1-Antitrypsin - metabolism Angiogenesis Biglycan - metabolism Biochemistry Biology and Life Sciences Blotting, Western Calgranulin A - genetics Calgranulin A - metabolism Calgranulin B - genetics Calgranulin B - metabolism Chemokine CXCL1 - metabolism Chymase Conjunctiva Cornea DNA microarrays Epidemiology Extracellular matrix Fibroblasts Fibrosis Fluorescent Antibody Technique Gelsolin Gelsolin - metabolism Gene expression Gene Expression Regulation - genetics Genes Genomes Genomics Humans Inflammation Medicine and Health Sciences Oligonucleotide Array Sequence Analysis Pathogenesis Patients Peptidase Protein arrays Proteins Proteomics Proteomics - methods Pterygium - genetics Pterygium - metabolism Real-Time Polymerase Chain Reaction RNA polymerase Sawdust Signal Transduction - genetics Signaling Tissues Transcription Transferrin Transferrin - metabolism U.V. radiation Ultraviolet radiation Vimentin Vimentin - metabolism |
| title | Evaluation of global differential gene and protein expression in primary Pterygium: S100A8 and S100A9 as possible drivers of a signaling network |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T17%3A45%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20global%20differential%20gene%20and%20protein%20expression%20in%20primary%20Pterygium:%20S100A8%20and%20S100A9%20as%20possible%20drivers%20of%20a%20signaling%20network&rft.jtitle=PloS%20one&rft.au=Hou,%20Aihua&rft.date=2014-05-13&rft.volume=9&rft.issue=5&rft.spage=e97402&rft.epage=e97402&rft.pages=e97402-e97402&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0097402&rft_dat=%3Cproquest_plos_%3E3302519471%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1524225195&rft_id=info:pmid/24825356&rft_doaj_id=oai_doaj_org_article_31f9e314bf0d4d249c9c859433b2839d&rfr_iscdi=true |