Quantitative measurement of brightness from living cells in the presence of photodepletion

The brightness of fluorescently labeled proteins provides an excellent marker for identifying protein interactions in living cells. Quantitative interpretation of brightness, however, hinges on a detailed understanding of the processes that affect the signal fluctuation of the fluorescent label. Her...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2014-05, Vol.9 (5), p.e97440-e97440
Hauptverfasser:	Hur, Kwang-Ho, Macdonald, Patrick J, Berk, Serkan, Angert, C Isaac, Chen, Yan, Mueller, Joachim D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Astronomy Bias Biology and Life Sciences Biomedical engineering Brightness Cell Line Cell Survival Cells (biology) Data processing Experiments Fluorescence Green Fluorescent Proteins - chemistry Humans Light Measurement Photobleaching Physical Sciences Physics Power Protein interaction Protein Multimerization Protein Structure, Quaternary Proteins Saccharomyces cerevisiae - cytology Signal processing Spectrometry, Fluorescence - methods Spectrum analysis Variations Yeast
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!