Stac3 inhibits myoblast differentiation into myotubes
The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. In this study we determined the potential role of Stac3 i...
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| Veröffentlicht in: | PloS one 2014-04, Vol.9 (4), p.e95926-e95926 |
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| creator | Ge, Xiaomei Zhang, Yafei Park, Sungwon Cong, Xiaofei Gerrard, David E Jiang, Honglin |
| description | The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. In this study we determined the potential role of Stac3 in myoblast proliferation and differentiation, two important steps of muscle development. Neither siRNA-mediated Stac3 knockdown nor plasmid-mediated Stac3 overexpression affected the proliferation of C2C12 myoblasts. Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. In contrast, Stac3 overexpression inhibited the differentiation of C2C12 myoblasts into myotubes as evidenced by decreased fusion index, decreased number of nuclei per myotube, and decreased mRNA and protein expression of myogenic markers. Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. Collectively, these data suggest an inhibitory role of endogenous Stac3 in myoblast differentiation. Myogenesis is a tightly controlled program; myofibers formed from prematurely differentiated myoblasts are dysfunctional. Thus, Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development. |
| doi_str_mv | 10.1371/journal.pone.0095926 |
| format | Article |
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In this study we determined the potential role of Stac3 in myoblast proliferation and differentiation, two important steps of muscle development. Neither siRNA-mediated Stac3 knockdown nor plasmid-mediated Stac3 overexpression affected the proliferation of C2C12 myoblasts. Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. In contrast, Stac3 overexpression inhibited the differentiation of C2C12 myoblasts into myotubes as evidenced by decreased fusion index, decreased number of nuclei per myotube, and decreased mRNA and protein expression of myogenic markers. Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. Collectively, these data suggest an inhibitory role of endogenous Stac3 in myoblast differentiation. Myogenesis is a tightly controlled program; myofibers formed from prematurely differentiated myoblasts are dysfunctional. Thus, Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0095926</identifier><identifier>PMID: 24788338</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Analysis ; Animals ; Base Sequence ; Biology and Life Sciences ; Cell Differentiation - physiology ; Cell Line ; Contraction ; Differentiation ; DNA Primers ; Embryos ; Gene expression ; Health aspects ; Insulin-like growth factors ; Markers ; Mice ; Muscle contraction ; Muscle Fibers, Skeletal - cytology ; Muscle proteins ; Muscles ; Musculoskeletal system ; Myoblasts ; Myoblasts - cytology ; Myogenesis ; Myogenin ; Myosin ; Myotubes ; Nerve Tissue Proteins - physiology ; Nuclei ; Physiological aspects ; Physiology ; Polymerase Chain Reaction ; Poultry ; Proteins ; RNA ; Rodents ; Signal transduction ; siRNA ; Skeletal muscle ; Zebrafish</subject><ispartof>PloS one, 2014-04, Vol.9 (4), p.e95926-e95926</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Ge et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Ge et al 2014 Ge et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c653t-3150371b5dea89baed1830a3eaf4d7902a28b449e542eb3746c73bccaccb514b3</citedby><cites>FETCH-LOGICAL-c653t-3150371b5dea89baed1830a3eaf4d7902a28b449e542eb3746c73bccaccb514b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005754/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005754/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24788338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ge, Xiaomei</creatorcontrib><creatorcontrib>Zhang, Yafei</creatorcontrib><creatorcontrib>Park, Sungwon</creatorcontrib><creatorcontrib>Cong, Xiaofei</creatorcontrib><creatorcontrib>Gerrard, David E</creatorcontrib><creatorcontrib>Jiang, Honglin</creatorcontrib><title>Stac3 inhibits myoblast differentiation into myotubes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. In this study we determined the potential role of Stac3 in myoblast proliferation and differentiation, two important steps of muscle development. Neither siRNA-mediated Stac3 knockdown nor plasmid-mediated Stac3 overexpression affected the proliferation of C2C12 myoblasts. Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. In contrast, Stac3 overexpression inhibited the differentiation of C2C12 myoblasts into myotubes as evidenced by decreased fusion index, decreased number of nuclei per myotube, and decreased mRNA and protein expression of myogenic markers. Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. Collectively, these data suggest an inhibitory role of endogenous Stac3 in myoblast differentiation. Myogenesis is a tightly controlled program; myofibers formed from prematurely differentiated myoblasts are dysfunctional. Thus, Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development.</description><subject>Analysis</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biology and Life Sciences</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line</subject><subject>Contraction</subject><subject>Differentiation</subject><subject>DNA Primers</subject><subject>Embryos</subject><subject>Gene expression</subject><subject>Health aspects</subject><subject>Insulin-like growth factors</subject><subject>Markers</subject><subject>Mice</subject><subject>Muscle contraction</subject><subject>Muscle Fibers, Skeletal - cytology</subject><subject>Muscle proteins</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Myoblasts</subject><subject>Myoblasts - cytology</subject><subject>Myogenesis</subject><subject>Myogenin</subject><subject>Myosin</subject><subject>Myotubes</subject><subject>Nerve Tissue Proteins - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Xiaomei</au><au>Zhang, Yafei</au><au>Park, Sungwon</au><au>Cong, Xiaofei</au><au>Gerrard, David E</au><au>Jiang, Honglin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stac3 inhibits myoblast differentiation into myotubes</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>9</volume><issue>4</issue><spage>e95926</spage><epage>e95926</epage><pages>e95926-e95926</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The functionally undefined Stac3 gene, predicted to encode a SH3 domain- and C1 domain-containing protein, was recently found to be specifically expressed in skeletal muscle and essential to normal skeletal muscle development and contraction. In this study we determined the potential role of Stac3 in myoblast proliferation and differentiation, two important steps of muscle development. Neither siRNA-mediated Stac3 knockdown nor plasmid-mediated Stac3 overexpression affected the proliferation of C2C12 myoblasts. Stac3 knockdown promoted the differentiation of C2C12 myoblasts into myotubes as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA and protein expression of myogenic markers including myogenin and myosin heavy chain. In contrast, Stac3 overexpression inhibited the differentiation of C2C12 myoblasts into myotubes as evidenced by decreased fusion index, decreased number of nuclei per myotube, and decreased mRNA and protein expression of myogenic markers. Compared to wild-type myoblasts, myoblasts from Stac3 knockout mouse embryos showed accelerated differentiation into myotubes in culture as evidenced by increased fusion index, increased number of nuclei per myotube, and increased mRNA expression of myogenic markers. Collectively, these data suggest an inhibitory role of endogenous Stac3 in myoblast differentiation. Myogenesis is a tightly controlled program; myofibers formed from prematurely differentiated myoblasts are dysfunctional. Thus, Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24788338</pmid><doi>10.1371/journal.pone.0095926</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Animals Base Sequence Biology and Life Sciences Cell Differentiation - physiology Cell Line Contraction Differentiation DNA Primers Embryos Gene expression Health aspects Insulin-like growth factors Markers Mice Muscle contraction Muscle Fibers, Skeletal - cytology Muscle proteins Muscles Musculoskeletal system Myoblasts Myoblasts - cytology Myogenesis Myogenin Myosin Myotubes Nerve Tissue Proteins - physiology Nuclei Physiological aspects Physiology Polymerase Chain Reaction Poultry Proteins RNA Rodents Signal transduction siRNA Skeletal muscle Zebrafish |
| title | Stac3 inhibits myoblast differentiation into myotubes |
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