Characterization and function of the first antibiotic isolated from a vent organism: the extremophile metazoan Alvinella pompejana
The emblematic hydrothermal worm Alvinella pompejana is one of the most thermo tolerant animal known on Earth. It relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. Here, by studying the Pompeii...
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| Veröffentlicht in: | PloS one 2014-04, Vol.9 (4), p.e95737 |
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| creator | Tasiemski, Aurélie Jung, Sascha Boidin-Wichlacz, Céline Jollivet, Didier Cuvillier-Hot, Virginie Pradillon, Florence Vetriani, Costantino Hecht, Oliver Sönnichsen, Frank D Gelhaus, Christoph Hung, Chien-Wen Tholey, Andreas Leippe, Matthias Grötzinger, Joachim Gaill, Françoise |
| description | The emblematic hydrothermal worm Alvinella pompejana is one of the most thermo tolerant animal known on Earth. It relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. Here, by studying the Pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. After purification and peptide sequencing, both the gene and the peptide tertiary structures were elucidated. As epibionts are not cultivated so far and because of lethal decompression effects upon Alvinella sampling, we developed shipboard biological assays to demonstrate that in addition to act in the first line of defense against microbial invasion, alvinellacin shapes and controls the worm's epibiotic microflora. Our results provide insights into the nature of an abyssal antimicrobial peptide (AMP) and into the manner in which an extremophile eukaryote uses it to interact with the particular microbial community of the hydrothermal vent ecosystem. Unlike earlier studies done on hydrothermal vents that all focused on the microbial side of the symbiosis, our work gives a view of this interaction from the host side. |
| doi_str_mv | 10.1371/journal.pone.0095737 |
| format | Article |
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It relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. Here, by studying the Pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. After purification and peptide sequencing, both the gene and the peptide tertiary structures were elucidated. As epibionts are not cultivated so far and because of lethal decompression effects upon Alvinella sampling, we developed shipboard biological assays to demonstrate that in addition to act in the first line of defense against microbial invasion, alvinellacin shapes and controls the worm's epibiotic microflora. Our results provide insights into the nature of an abyssal antimicrobial peptide (AMP) and into the manner in which an extremophile eukaryote uses it to interact with the particular microbial community of the hydrothermal vent ecosystem. 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tasiemski, Aurélie</au><au>Jung, Sascha</au><au>Boidin-Wichlacz, Céline</au><au>Jollivet, Didier</au><au>Cuvillier-Hot, Virginie</au><au>Pradillon, Florence</au><au>Vetriani, Costantino</au><au>Hecht, Oliver</au><au>Sönnichsen, Frank D</au><au>Gelhaus, Christoph</au><au>Hung, Chien-Wen</au><au>Tholey, Andreas</au><au>Leippe, Matthias</au><au>Grötzinger, Joachim</au><au>Gaill, Françoise</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and function of the first antibiotic isolated from a vent organism: the extremophile metazoan Alvinella pompejana</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>9</volume><issue>4</issue><spage>e95737</spage><pages>e95737-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The emblematic hydrothermal worm Alvinella pompejana is one of the most thermo tolerant animal known on Earth. It relies on a symbiotic association offering a unique opportunity to discover biochemical adaptations that allow animals to thrive in such a hostile habitat. Here, by studying the Pompeii worm, we report on the discovery of the first antibiotic peptide from a deep-sea organism, namely alvinellacin. After purification and peptide sequencing, both the gene and the peptide tertiary structures were elucidated. As epibionts are not cultivated so far and because of lethal decompression effects upon Alvinella sampling, we developed shipboard biological assays to demonstrate that in addition to act in the first line of defense against microbial invasion, alvinellacin shapes and controls the worm's epibiotic microflora. Our results provide insights into the nature of an abyssal antimicrobial peptide (AMP) and into the manner in which an extremophile eukaryote uses it to interact with the particular microbial community of the hydrothermal vent ecosystem. Unlike earlier studies done on hydrothermal vents that all focused on the microbial side of the symbiosis, our work gives a view of this interaction from the host side.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24776651</pmid><doi>10.1371/journal.pone.0095737</doi><orcidid>https://orcid.org/0000-0003-3559-5115</orcidid><orcidid>https://orcid.org/0000-0002-0242-868X</orcidid><orcidid>https://orcid.org/0000-0002-6473-6290</orcidid><orcidid>https://orcid.org/0000-0002-3433-1110</orcidid><orcidid>https://orcid.org/0000-0001-8042-1857</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-04, Vol.9 (4), p.e95737 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1519577633 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry; Public Library of Science (PLoS) |
| subjects | Abyssal zone Adaptation Adaptations Amino Acid Sequence Analysis Animals Antibiotics Antimicrobial Cationic Peptides - chemistry Antimicrobial Cationic Peptides - isolation & purification Antimicrobial Cationic Peptides - pharmacology Bacteria Biochemistry Biochemistry, Molecular Biology Biological effects Biology and Life Sciences Decompression Deep sea Dementia Distribution Ecology and Environmental Sciences Ecosystem Epibionts Evolution, Molecular Gene sequencing Genomics Hydrothermal plumes Hydrothermal systems (Geology) Hydrothermal vent ecology Hydrothermal vents Hydrothermal Vents - microbiology Life Sciences Microbiology and Parasitology Microbiota (Symbiotic organisms) Microflora Microorganisms Models, Molecular Molecular Sequence Data Organisms Peptides Polychaeta - chemistry Protein Structure, Secondary Purification Studies Symbiosis Vents Zoology |
| title | Characterization and function of the first antibiotic isolated from a vent organism: the extremophile metazoan Alvinella pompejana |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T00%3A57%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20and%20function%20of%20the%20first%20antibiotic%20isolated%20from%20a%20vent%20organism:%20the%20extremophile%20metazoan%20Alvinella%20pompejana&rft.jtitle=PloS%20one&rft.au=Tasiemski,%20Aur%C3%A9lie&rft.date=2014-04-01&rft.volume=9&rft.issue=4&rft.spage=e95737&rft.pages=e95737-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0095737&rft_dat=%3Cgale_plos_%3EA375585021%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1519577633&rft_id=info:pmid/24776651&rft_galeid=A375585021&rft_doaj_id=oai_doaj_org_article_3cd4fb95e1f94b43976e474b0aa6497a&rfr_iscdi=true |