Trypanosoma cruzi infection down-modulates the immunoproteasome biosynthesis and the MHC class I cell surface expression in HeLa cells
Generally, Trypanosoma cruzi infection in human is persistent and tends to chronicity, suggesting that the parasite evade the immune surveillance by down regulating the intracellular antigen processing routes. Within the MHC class I pathway, the majority of antigenic peptides are generated by the pr...
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| description | Generally, Trypanosoma cruzi infection in human is persistent and tends to chronicity, suggesting that the parasite evade the immune surveillance by down regulating the intracellular antigen processing routes. Within the MHC class I pathway, the majority of antigenic peptides are generated by the proteasome. However, upon IFN-γ stimulation, the catalytic constitutive subunits of the proteasome are replaced by the subunits β1i/LMP2, β2i/MECL-1 and β5i/LMP7 to form the immunoproteasome. In this scenario, we analyzed whether the expression and activity of the constitutive and the immunoproteasome as well as the expression of other components of the MHC class I pathway are altered during the infection of HeLa cells with T. cruzi. By RT-PCR and two-dimensional gel electrophoresis analysis, we showed that the expression and composition of the constitutive proteasome is not affected by the parasite. In contrast, the biosynthesis of the β1i, β2i, β5i immunosubunits, PA28β, TAP1 and the MHC class I molecule as well as the proteasomal proteolytic activities were down-regulated in infected-IFN-γ-treated cell cultures. Taken together, our results provide evidence that the protozoan T. cruzi specifically modulates its infection through an unknown posttranscriptional mechanism that inhibits the expression of the MHC class I pathway components. |
| doi_str_mv | 10.1371/journal.pone.0095977 |
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Within the MHC class I pathway, the majority of antigenic peptides are generated by the proteasome. However, upon IFN-γ stimulation, the catalytic constitutive subunits of the proteasome are replaced by the subunits β1i/LMP2, β2i/MECL-1 and β5i/LMP7 to form the immunoproteasome. In this scenario, we analyzed whether the expression and activity of the constitutive and the immunoproteasome as well as the expression of other components of the MHC class I pathway are altered during the infection of HeLa cells with T. cruzi. By RT-PCR and two-dimensional gel electrophoresis analysis, we showed that the expression and composition of the constitutive proteasome is not affected by the parasite. In contrast, the biosynthesis of the β1i, β2i, β5i immunosubunits, PA28β, TAP1 and the MHC class I molecule as well as the proteasomal proteolytic activities were down-regulated in infected-IFN-γ-treated cell cultures. Taken together, our results provide evidence that the protozoan T. cruzi specifically modulates its infection through an unknown posttranscriptional mechanism that inhibits the expression of the MHC class I pathway components.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0095977</identifier><identifier>PMID: 24752321</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adenoviruses ; Antigen presentation ; Antigen processing ; Antigens ; Biology and Life Sciences ; Biosynthesis ; Catalysis ; Cell surface ; Chagas Disease - metabolism ; Chagas Disease - physiopathology ; Chagas' disease ; Drug therapy ; Gel electrophoresis ; Genes, MHC Class I - genetics ; Health aspects ; HeLa Cells ; Humans ; Immunoprecipitation ; Immunosurveillance ; Infections ; Interferon ; Interferon-gamma - pharmacology ; Lymphocytes ; Major histocompatibility complex ; Medicine and Health Sciences ; Parasites ; Peptides ; Physiological aspects ; Polymerase chain reaction ; Post-transcription ; Proteasome Endopeptidase Complex - metabolism ; Proteasomes ; Proteins ; Proteolysis ; Protozoa ; Reverse Transcriptase Polymerase Chain Reaction ; Trypanosoma cruzi ; Trypanosoma cruzi - pathogenicity ; Tumor necrosis factor-TNF ; Two dimensional analysis ; γ-Interferon</subject><ispartof>PloS one, 2014-04, Vol.9 (4), p.e95977-e95977</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Camargo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Taken together, our results provide evidence that the protozoan T. cruzi specifically modulates its infection through an unknown posttranscriptional mechanism that inhibits the expression of the MHC class I pathway components.</description><subject>Adenoviruses</subject><subject>Antigen presentation</subject><subject>Antigen processing</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Biosynthesis</subject><subject>Catalysis</subject><subject>Cell surface</subject><subject>Chagas Disease - metabolism</subject><subject>Chagas Disease - physiopathology</subject><subject>Chagas' disease</subject><subject>Drug therapy</subject><subject>Gel electrophoresis</subject><subject>Genes, MHC Class I - genetics</subject><subject>Health aspects</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Immunosurveillance</subject><subject>Infections</subject><subject>Interferon</subject><subject>Interferon-gamma - 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Within the MHC class I pathway, the majority of antigenic peptides are generated by the proteasome. However, upon IFN-γ stimulation, the catalytic constitutive subunits of the proteasome are replaced by the subunits β1i/LMP2, β2i/MECL-1 and β5i/LMP7 to form the immunoproteasome. In this scenario, we analyzed whether the expression and activity of the constitutive and the immunoproteasome as well as the expression of other components of the MHC class I pathway are altered during the infection of HeLa cells with T. cruzi. By RT-PCR and two-dimensional gel electrophoresis analysis, we showed that the expression and composition of the constitutive proteasome is not affected by the parasite. In contrast, the biosynthesis of the β1i, β2i, β5i immunosubunits, PA28β, TAP1 and the MHC class I molecule as well as the proteasomal proteolytic activities were down-regulated in infected-IFN-γ-treated cell cultures. Taken together, our results provide evidence that the protozoan T. cruzi specifically modulates its infection through an unknown posttranscriptional mechanism that inhibits the expression of the MHC class I pathway components.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24752321</pmid><doi>10.1371/journal.pone.0095977</doi><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adenoviruses Antigen presentation Antigen processing Antigens Biology and Life Sciences Biosynthesis Catalysis Cell surface Chagas Disease - metabolism Chagas Disease - physiopathology Chagas' disease Drug therapy Gel electrophoresis Genes, MHC Class I - genetics Health aspects HeLa Cells Humans Immunoprecipitation Immunosurveillance Infections Interferon Interferon-gamma - pharmacology Lymphocytes Major histocompatibility complex Medicine and Health Sciences Parasites Peptides Physiological aspects Polymerase chain reaction Post-transcription Proteasome Endopeptidase Complex - metabolism Proteasomes Proteins Proteolysis Protozoa Reverse Transcriptase Polymerase Chain Reaction Trypanosoma cruzi Trypanosoma cruzi - pathogenicity Tumor necrosis factor-TNF Two dimensional analysis γ-Interferon |
| title | Trypanosoma cruzi infection down-modulates the immunoproteasome biosynthesis and the MHC class I cell surface expression in HeLa cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T03%3A44%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Trypanosoma%20cruzi%20infection%20down-modulates%20the%20immunoproteasome%20biosynthesis%20and%20the%20MHC%20class%20I%20cell%20surface%20expression%20in%20HeLa%20cells&rft.jtitle=PloS%20one&rft.au=Camargo,%20Ricardo&rft.date=2014-04-01&rft.volume=9&rft.issue=4&rft.spage=e95977&rft.epage=e95977&rft.pages=e95977-e95977&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0095977&rft_dat=%3Cgale_plos_%3EA375585197%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1518097445&rft_id=info:pmid/24752321&rft_galeid=A375585197&rft_doaj_id=oai_doaj_org_article_501a6b8aba65450ea39d9a454900fdd9&rfr_iscdi=true |