The role of 1.5 tesla MRI and anesthetic regimen concerning cardiac analysis in mice with cardiomyopathy
Accurate assessment of left ventricular function in rodent models is essential for the evaluation of new therapeutic approaches for cardiac diseases. In our study, we provide new insights regarding the role of a 1.5 Tesla (T) magnetic resonance imaging (MRI) device and different anesthetic regimens...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-04, Vol.9 (4), p.e94615 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 4 |
| container_start_page | e94615 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Grabmaier, Ulrich Theiss, Hans D Keithahn, Alexandra Kreiner, Julia Brenner, Christoph Huber, Bruno von der Helm, Christine Gross, Lisa Klingel, Karin Franz, Wolfgang-M Brunner, Stefan |
| description | Accurate assessment of left ventricular function in rodent models is essential for the evaluation of new therapeutic approaches for cardiac diseases. In our study, we provide new insights regarding the role of a 1.5 Tesla (T) magnetic resonance imaging (MRI) device and different anesthetic regimens on data validity. As dedicated small animal MRI and echocardiographic devices are not broadly available, we evaluated whether monitoring cardiac function in small rodents with a clinical 1.5 T MRI device is feasible. On a clinical electrocardiogram (ECG) synchronized 1.5 T MRI scanner we therefore studied cardiac function parameters of mice with chronic virus-induced cardiomyopathy. Thus, reduced left ventricular ejection fraction (LVEF) could be verified compared to healthy controls. However, our results showed a high variability. First, anesthesia with medetomidine, midazolam and fentanyl (MMF) led to depressed cardiac function parameters and more variability than isoflurane gas inhalation anesthesia, especially at high concentrations. Furthermore, calculation of an average ejection fraction value from sequenced scans significantly reduced the variance of the results. To sum up, we introduce the clinical 1.5 T MRI device as a new tool for effective analysis of left ventricular function in mice with cardiomyopathy. Besides, we suggest isoflurane gas inhalation anesthesia at high concentrations for variance reduction and recommend calculation of an average ejection fraction value from multiple sequenced MRI scans to provide valid data and a solid basis for further clinical testing. |
| doi_str_mv | 10.1371/journal.pone.0094615 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1517611365</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A375583787</galeid><doaj_id>oai_doaj_org_article_f2220c26dfaa4b3b9525ddd8fc9b29cb</doaj_id><sourcerecordid>A375583787</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-9efae642f994ce199800c8e5af040135321e122d7e9619eea45f5fa8a69f535d3</originalsourceid><addsrcrecordid>eNqNk12L1DAUhoso7rr6D0QLgujFjE3SpM2NsCx-DKwsrKu3IZOetFnSZDZp1fn3Zna6y1T2QkpoSJ7znpw3OVn2EhVLRCr04dqPwUm73HgHy6LgJUP0UXaMOMELhgvy-GB-lD2L8booKKkZe5od4bIqqxqx46y76iAP3kLudY6WNB8gWpl_u1zl0jVpQBw6GIzKA7SmB5cr7xQEZ1ybKxkaI1WipN1GE3Pj8t4oyH-bodvv-n7rN3Lots-zJ1raCC-m_0n24_Onq7Ovi_OLL6uz0_OFYhwPCw5aAiux5rxUgDivi0LVQKUuygIRSjAChHFTAWeIA8iSaqplLRnXlNCGnGSv97ob66OYTIoCUVQxhAijiVjticbLa7EJppdhK7w04nbBh1bIkCq2IDTGuFCYNVrKck3WnGLaNE2tFV9jrtZJ6-OUbVz30ChwQ5B2JjrfcaYTrf8lCOeI4SoJvJsEgr8Zk9miN1GBtcl5P96eu8Ylqunu3G_-QR-ubqJamQowTvuUV-1ExSmpKK1JVe_SLh-g0tdAusD0orRJ67OA97OAxAzwZ2jlGKNYfb_8f_bi55x9e8B2IO3QRW_HwXgX52C5B1XwMQbQ9yajQuwa4s4NsWsIMTVECnt1eEH3QXcdQP4CeicFzg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1517611365</pqid></control><display><type>article</type><title>The role of 1.5 tesla MRI and anesthetic regimen concerning cardiac analysis in mice with cardiomyopathy</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Grabmaier, Ulrich ; Theiss, Hans D ; Keithahn, Alexandra ; Kreiner, Julia ; Brenner, Christoph ; Huber, Bruno ; von der Helm, Christine ; Gross, Lisa ; Klingel, Karin ; Franz, Wolfgang-M ; Brunner, Stefan</creator><creatorcontrib>Grabmaier, Ulrich ; Theiss, Hans D ; Keithahn, Alexandra ; Kreiner, Julia ; Brenner, Christoph ; Huber, Bruno ; von der Helm, Christine ; Gross, Lisa ; Klingel, Karin ; Franz, Wolfgang-M ; Brunner, Stefan</creatorcontrib><description>Accurate assessment of left ventricular function in rodent models is essential for the evaluation of new therapeutic approaches for cardiac diseases. In our study, we provide new insights regarding the role of a 1.5 Tesla (T) magnetic resonance imaging (MRI) device and different anesthetic regimens on data validity. As dedicated small animal MRI and echocardiographic devices are not broadly available, we evaluated whether monitoring cardiac function in small rodents with a clinical 1.5 T MRI device is feasible. On a clinical electrocardiogram (ECG) synchronized 1.5 T MRI scanner we therefore studied cardiac function parameters of mice with chronic virus-induced cardiomyopathy. Thus, reduced left ventricular ejection fraction (LVEF) could be verified compared to healthy controls. However, our results showed a high variability. First, anesthesia with medetomidine, midazolam and fentanyl (MMF) led to depressed cardiac function parameters and more variability than isoflurane gas inhalation anesthesia, especially at high concentrations. Furthermore, calculation of an average ejection fraction value from sequenced scans significantly reduced the variance of the results. To sum up, we introduce the clinical 1.5 T MRI device as a new tool for effective analysis of left ventricular function in mice with cardiomyopathy. Besides, we suggest isoflurane gas inhalation anesthesia at high concentrations for variance reduction and recommend calculation of an average ejection fraction value from multiple sequenced MRI scans to provide valid data and a solid basis for further clinical testing.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0094615</identifier><identifier>PMID: 24747816</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Anesthesia ; Anesthetics ; Anesthetics - pharmacology ; Animal models ; Animals ; Biology and Life Sciences ; Cardiomyopathies - physiopathology ; Cardiomyopathy ; Coronary artery disease ; Diagnosis ; Dose-Response Relationship, Drug ; Echocardiography ; EKG ; Fentanyl ; Health aspects ; Heart - drug effects ; Heart - physiopathology ; Heart attacks ; Heart diseases ; Heart failure ; Heart Function Tests - instrumentation ; Heart Function Tests - methods ; Inflammation ; Inhalation ; Internal medicine ; Ischemia ; Isoflurane ; Laboratory animals ; Magnetic resonance ; Magnetic Resonance Imaging ; Male ; Medicine ; Medicine and Health Sciences ; Mice ; Microscopy ; Midazolam ; NMR ; Nuclear magnetic resonance ; Research and Analysis Methods ; Respiration ; Rodents ; Scanners ; Stroke Volume - drug effects ; Variability ; Ventricle ; Viral infections ; Viruses</subject><ispartof>PloS one, 2014-04, Vol.9 (4), p.e94615</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Grabmaier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Grabmaier et al 2014 Grabmaier et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-9efae642f994ce199800c8e5af040135321e122d7e9619eea45f5fa8a69f535d3</citedby><cites>FETCH-LOGICAL-c692t-9efae642f994ce199800c8e5af040135321e122d7e9619eea45f5fa8a69f535d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991627/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991627/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24747816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grabmaier, Ulrich</creatorcontrib><creatorcontrib>Theiss, Hans D</creatorcontrib><creatorcontrib>Keithahn, Alexandra</creatorcontrib><creatorcontrib>Kreiner, Julia</creatorcontrib><creatorcontrib>Brenner, Christoph</creatorcontrib><creatorcontrib>Huber, Bruno</creatorcontrib><creatorcontrib>von der Helm, Christine</creatorcontrib><creatorcontrib>Gross, Lisa</creatorcontrib><creatorcontrib>Klingel, Karin</creatorcontrib><creatorcontrib>Franz, Wolfgang-M</creatorcontrib><creatorcontrib>Brunner, Stefan</creatorcontrib><title>The role of 1.5 tesla MRI and anesthetic regimen concerning cardiac analysis in mice with cardiomyopathy</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Accurate assessment of left ventricular function in rodent models is essential for the evaluation of new therapeutic approaches for cardiac diseases. In our study, we provide new insights regarding the role of a 1.5 Tesla (T) magnetic resonance imaging (MRI) device and different anesthetic regimens on data validity. As dedicated small animal MRI and echocardiographic devices are not broadly available, we evaluated whether monitoring cardiac function in small rodents with a clinical 1.5 T MRI device is feasible. On a clinical electrocardiogram (ECG) synchronized 1.5 T MRI scanner we therefore studied cardiac function parameters of mice with chronic virus-induced cardiomyopathy. Thus, reduced left ventricular ejection fraction (LVEF) could be verified compared to healthy controls. However, our results showed a high variability. First, anesthesia with medetomidine, midazolam and fentanyl (MMF) led to depressed cardiac function parameters and more variability than isoflurane gas inhalation anesthesia, especially at high concentrations. Furthermore, calculation of an average ejection fraction value from sequenced scans significantly reduced the variance of the results. To sum up, we introduce the clinical 1.5 T MRI device as a new tool for effective analysis of left ventricular function in mice with cardiomyopathy. Besides, we suggest isoflurane gas inhalation anesthesia at high concentrations for variance reduction and recommend calculation of an average ejection fraction value from multiple sequenced MRI scans to provide valid data and a solid basis for further clinical testing.</description><subject>Anesthesia</subject><subject>Anesthetics</subject><subject>Anesthetics - pharmacology</subject><subject>Animal models</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Cardiomyopathies - physiopathology</subject><subject>Cardiomyopathy</subject><subject>Coronary artery disease</subject><subject>Diagnosis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Echocardiography</subject><subject>EKG</subject><subject>Fentanyl</subject><subject>Health aspects</subject><subject>Heart - drug effects</subject><subject>Heart - physiopathology</subject><subject>Heart attacks</subject><subject>Heart diseases</subject><subject>Heart failure</subject><subject>Heart Function Tests - instrumentation</subject><subject>Heart Function Tests - methods</subject><subject>Inflammation</subject><subject>Inhalation</subject><subject>Internal medicine</subject><subject>Ischemia</subject><subject>Isoflurane</subject><subject>Laboratory animals</subject><subject>Magnetic resonance</subject><subject>Magnetic Resonance Imaging</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Midazolam</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Research and Analysis Methods</subject><subject>Respiration</subject><subject>Rodents</subject><subject>Scanners</subject><subject>Stroke Volume - drug effects</subject><subject>Variability</subject><subject>Ventricle</subject><subject>Viral infections</subject><subject>Viruses</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjE3SpM2NsCx-DKwsrKu3IZOetFnSZDZp1fn3Zna6y1T2QkpoSJ7znpw3OVn2EhVLRCr04dqPwUm73HgHy6LgJUP0UXaMOMELhgvy-GB-lD2L8booKKkZe5od4bIqqxqx46y76iAP3kLudY6WNB8gWpl_u1zl0jVpQBw6GIzKA7SmB5cr7xQEZ1ybKxkaI1WipN1GE3Pj8t4oyH-bodvv-n7rN3Lots-zJ1raCC-m_0n24_Onq7Ovi_OLL6uz0_OFYhwPCw5aAiux5rxUgDivi0LVQKUuygIRSjAChHFTAWeIA8iSaqplLRnXlNCGnGSv97ob66OYTIoCUVQxhAijiVjticbLa7EJppdhK7w04nbBh1bIkCq2IDTGuFCYNVrKck3WnGLaNE2tFV9jrtZJ6-OUbVz30ChwQ5B2JjrfcaYTrf8lCOeI4SoJvJsEgr8Zk9miN1GBtcl5P96eu8Ylqunu3G_-QR-ubqJamQowTvuUV-1ExSmpKK1JVe_SLh-g0tdAusD0orRJ67OA97OAxAzwZ2jlGKNYfb_8f_bi55x9e8B2IO3QRW_HwXgX52C5B1XwMQbQ9yajQuwa4s4NsWsIMTVECnt1eEH3QXcdQP4CeicFzg</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Grabmaier, Ulrich</creator><creator>Theiss, Hans D</creator><creator>Keithahn, Alexandra</creator><creator>Kreiner, Julia</creator><creator>Brenner, Christoph</creator><creator>Huber, Bruno</creator><creator>von der Helm, Christine</creator><creator>Gross, Lisa</creator><creator>Klingel, Karin</creator><creator>Franz, Wolfgang-M</creator><creator>Brunner, Stefan</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140401</creationdate><title>The role of 1.5 tesla MRI and anesthetic regimen concerning cardiac analysis in mice with cardiomyopathy</title><author>Grabmaier, Ulrich ; Theiss, Hans D ; Keithahn, Alexandra ; Kreiner, Julia ; Brenner, Christoph ; Huber, Bruno ; von der Helm, Christine ; Gross, Lisa ; Klingel, Karin ; Franz, Wolfgang-M ; Brunner, Stefan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-9efae642f994ce199800c8e5af040135321e122d7e9619eea45f5fa8a69f535d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anesthesia</topic><topic>Anesthetics</topic><topic>Anesthetics - pharmacology</topic><topic>Animal models</topic><topic>Animals</topic><topic>Biology and Life Sciences</topic><topic>Cardiomyopathies - physiopathology</topic><topic>Cardiomyopathy</topic><topic>Coronary artery disease</topic><topic>Diagnosis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Echocardiography</topic><topic>EKG</topic><topic>Fentanyl</topic><topic>Health aspects</topic><topic>Heart - drug effects</topic><topic>Heart - physiopathology</topic><topic>Heart attacks</topic><topic>Heart diseases</topic><topic>Heart failure</topic><topic>Heart Function Tests - instrumentation</topic><topic>Heart Function Tests - methods</topic><topic>Inflammation</topic><topic>Inhalation</topic><topic>Internal medicine</topic><topic>Ischemia</topic><topic>Isoflurane</topic><topic>Laboratory animals</topic><topic>Magnetic resonance</topic><topic>Magnetic Resonance Imaging</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine and Health Sciences</topic><topic>Mice</topic><topic>Microscopy</topic><topic>Midazolam</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Research and Analysis Methods</topic><topic>Respiration</topic><topic>Rodents</topic><topic>Scanners</topic><topic>Stroke Volume - drug effects</topic><topic>Variability</topic><topic>Ventricle</topic><topic>Viral infections</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grabmaier, Ulrich</creatorcontrib><creatorcontrib>Theiss, Hans D</creatorcontrib><creatorcontrib>Keithahn, Alexandra</creatorcontrib><creatorcontrib>Kreiner, Julia</creatorcontrib><creatorcontrib>Brenner, Christoph</creatorcontrib><creatorcontrib>Huber, Bruno</creatorcontrib><creatorcontrib>von der Helm, Christine</creatorcontrib><creatorcontrib>Gross, Lisa</creatorcontrib><creatorcontrib>Klingel, Karin</creatorcontrib><creatorcontrib>Franz, Wolfgang-M</creatorcontrib><creatorcontrib>Brunner, Stefan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grabmaier, Ulrich</au><au>Theiss, Hans D</au><au>Keithahn, Alexandra</au><au>Kreiner, Julia</au><au>Brenner, Christoph</au><au>Huber, Bruno</au><au>von der Helm, Christine</au><au>Gross, Lisa</au><au>Klingel, Karin</au><au>Franz, Wolfgang-M</au><au>Brunner, Stefan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of 1.5 tesla MRI and anesthetic regimen concerning cardiac analysis in mice with cardiomyopathy</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>9</volume><issue>4</issue><spage>e94615</spage><pages>e94615-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Accurate assessment of left ventricular function in rodent models is essential for the evaluation of new therapeutic approaches for cardiac diseases. In our study, we provide new insights regarding the role of a 1.5 Tesla (T) magnetic resonance imaging (MRI) device and different anesthetic regimens on data validity. As dedicated small animal MRI and echocardiographic devices are not broadly available, we evaluated whether monitoring cardiac function in small rodents with a clinical 1.5 T MRI device is feasible. On a clinical electrocardiogram (ECG) synchronized 1.5 T MRI scanner we therefore studied cardiac function parameters of mice with chronic virus-induced cardiomyopathy. Thus, reduced left ventricular ejection fraction (LVEF) could be verified compared to healthy controls. However, our results showed a high variability. First, anesthesia with medetomidine, midazolam and fentanyl (MMF) led to depressed cardiac function parameters and more variability than isoflurane gas inhalation anesthesia, especially at high concentrations. Furthermore, calculation of an average ejection fraction value from sequenced scans significantly reduced the variance of the results. To sum up, we introduce the clinical 1.5 T MRI device as a new tool for effective analysis of left ventricular function in mice with cardiomyopathy. Besides, we suggest isoflurane gas inhalation anesthesia at high concentrations for variance reduction and recommend calculation of an average ejection fraction value from multiple sequenced MRI scans to provide valid data and a solid basis for further clinical testing.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24747816</pmid><doi>10.1371/journal.pone.0094615</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-04, Vol.9 (4), p.e94615 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1517611365 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Anesthesia Anesthetics Anesthetics - pharmacology Animal models Animals Biology and Life Sciences Cardiomyopathies - physiopathology Cardiomyopathy Coronary artery disease Diagnosis Dose-Response Relationship, Drug Echocardiography EKG Fentanyl Health aspects Heart - drug effects Heart - physiopathology Heart attacks Heart diseases Heart failure Heart Function Tests - instrumentation Heart Function Tests - methods Inflammation Inhalation Internal medicine Ischemia Isoflurane Laboratory animals Magnetic resonance Magnetic Resonance Imaging Male Medicine Medicine and Health Sciences Mice Microscopy Midazolam NMR Nuclear magnetic resonance Research and Analysis Methods Respiration Rodents Scanners Stroke Volume - drug effects Variability Ventricle Viral infections Viruses |
| title | The role of 1.5 tesla MRI and anesthetic regimen concerning cardiac analysis in mice with cardiomyopathy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T03%3A25%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20role%20of%201.5%20tesla%20MRI%20and%20anesthetic%20regimen%20concerning%20cardiac%20analysis%20in%20mice%20with%20cardiomyopathy&rft.jtitle=PloS%20one&rft.au=Grabmaier,%20Ulrich&rft.date=2014-04-01&rft.volume=9&rft.issue=4&rft.spage=e94615&rft.pages=e94615-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0094615&rft_dat=%3Cgale_plos_%3EA375583787%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1517611365&rft_id=info:pmid/24747816&rft_galeid=A375583787&rft_doaj_id=oai_doaj_org_article_f2220c26dfaa4b3b9525ddd8fc9b29cb&rfr_iscdi=true |