Immobilised histidine tagged β2-adrenoceptor oriented by a diazonium salt reaction and its application in exploring drug-protein interaction using ephedrine and pseudoephedrine as probes

A new oriented method using a diazonium salt reaction was developed for linking β2-adrenoceptor (β2-AR) on the surface of macroporous silica gel. Stationary phase containing the immobilised receptor was used to investigate the interaction between β2-AR and ephedrine plus pseudoephedrine by zonal elu...

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Veröffentlicht in:	PloS one 2014-04, Vol.9 (4), p.e94955
Hauptverfasser:	Li, Qian, Bian, Liujiao, Zhao, Xinfeng, Gao, Xiaokang, Zheng, Jianbin, Li, Zijian, Zhang, Youyi, Jiang, Ru, Zheng, Xiaohui
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenergic receptors Animals Binding sites Binding, Competitive Chromatography Diazonium Compounds - chemistry Drugs Education Electrostatic properties Elution Ephedrine Ephedrine - metabolism Histidine Immobilized Proteins - chemistry Immobilized Proteins - metabolism Laboratories Methods Molecular Docking Simulation Protein Binding Protein Conformation Protein Stability Proteins Pseudoephedrine - metabolism Receptors, Adrenergic, beta-2 - chemistry Receptors, Adrenergic, beta-2 - metabolism Respiratory system Salts Salts - chemistry Silica Silica gel Silicon dioxide Silicon Dioxide - chemistry Stationary phase Studies Substrates Thermodynamics
Online-Zugang:	Volltext
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