Communities of endophytic sebacinales associated with roots of herbaceous plants in agricultural and grassland ecosystems are dominated by Serendipita herbamans sp. nov
Endophytic fungi are known to be commonly associated with herbaceous plants, however, there are few studies focusing on their occurrence and distribution in plant roots from ecosystems with different land uses. To explore the phylogenetic diversity and community structure of Sebacinales endophytes f...
Gespeichert in:
Veröffentlicht in: | PloS one 2014-04, Vol.9 (4), p.e94676-e94676 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | e94676 |
---|---|
container_issue | 4 |
container_start_page | e94676 |
container_title | PloS one |
container_volume | 9 |
creator | Riess, Kai Oberwinkler, Franz Bauer, Robert Garnica, Sigisfredo |
description | Endophytic fungi are known to be commonly associated with herbaceous plants, however, there are few studies focusing on their occurrence and distribution in plant roots from ecosystems with different land uses. To explore the phylogenetic diversity and community structure of Sebacinales endophytes from agricultural and grassland habitats under different land uses, we analysed the roots of herbaceous plants using strain isolation, polymerase chain reaction (PCR), transmission electron microscopy (TEM) and co-cultivation experiments. A new sebacinoid strain named Serendipita herbamans belonging to Sebacinales group B was isolated from the roots of Bistorta vivipara, which is characterized by colourless monilioid cells (chlamydospores) that become yellow with age. This species was very common and widely distributed in association with a broad spectrum of herbaceous plant families in diverse habitats, independent of land use type. Ultrastructurally, the presence of S. herbamans was detected in the cortical cells of Plantago media, Potentilla anserina and Triticum aestivum. In addition, 13 few frequent molecular operational taxonomic units (MOTUs) or species were found across agricultural and grassland habitats, which did not exhibit a distinctive phylogenetic structure. Laboratory-based assays indicate that S. herbamans has the ability to colonize fine roots and stimulate plant growth. Although endophytic Sebacinales are widely distributed across agricultural and grassland habitats, TEM and nested PCR analyses reinforce the observation that these microorganisms are present in low quantity in plant roots, with no evidence of host specificity. |
doi_str_mv | 10.1371/journal.pone.0094676 |
format | Article |
fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1517445132</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A375583839</galeid><doaj_id>oai_doaj_org_article_11a448b97a4d4653a1df0cb4cd4c182f</doaj_id><sourcerecordid>A375583839</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-dc95bf8405a53d0ba83aac4f29f598564cb028de19e11784ef03bcbcd27194503</originalsourceid><addsrcrecordid>eNqNk9-K1DAUxoso7rr6BqIBQfRixqRJ2vRGWBb_DCwsuOptSJO0zdAm3SRdnTfyMU1nZpcZ2QvJRcPJ73wn_U5Olr1EcIlwiT6s3eSt6Jejs3oJYUWKsniUnaIK54sih_jxwf4kexbCGkKKWVE8zU5yUhKMGD3N_ly4YZisiUYH4BqgrXJjt4lGgqBrIU0qkU5ECE4aEbUCv0zsgHcubvlO-0RpNwUw9sKmoLFAtN7IqY-TFz0QVoHWJ4F-3mnpwiZEPSRNr4FyQ6owy9YbcK19Km9GE8VOdxA2gDAugXW3z7MnjeiDfrH_nmU_Pn_6fvF1cXn1ZXVxfrmQRZXHhZIVrRtGIBUUK1gLhoWQpMmrhlaMFkTWMGdKo0ojVDKiG4hrWUuVl6giFOKz7PVOd-xd4HuTA0cUlYRQhPNErHaEcmLNR28G4TfcCcO3AedbLnwysNccIUEIq6tSEEUKigVSDZQ1kYpIxPImaX3cV5vqQSupbUyeHYken1jT8dbdclxVqZvzZd7tBby7mXSIfDBB6j6ZPTdle29WMsjKhL75B3347_ZUmxrPjW1cqitnUX6OS0oZZrhK1PIBKi2lByPTi2xMih8lvD9KSEzUv2MrphD46vrb_7NXP4_Ztwdsp0Ufu-D6KRpnwzFIdqD0LgSvm3uTEeTzQN25weeB4vuBSmmvDht0n3Q3QfgvQucfsw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1517445132</pqid></control><display><type>article</type><title>Communities of endophytic sebacinales associated with roots of herbaceous plants in agricultural and grassland ecosystems are dominated by Serendipita herbamans sp. nov</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Riess, Kai ; Oberwinkler, Franz ; Bauer, Robert ; Garnica, Sigisfredo</creator><contributor>Berg, Gabriele</contributor><creatorcontrib>Riess, Kai ; Oberwinkler, Franz ; Bauer, Robert ; Garnica, Sigisfredo ; Berg, Gabriele</creatorcontrib><description>Endophytic fungi are known to be commonly associated with herbaceous plants, however, there are few studies focusing on their occurrence and distribution in plant roots from ecosystems with different land uses. To explore the phylogenetic diversity and community structure of Sebacinales endophytes from agricultural and grassland habitats under different land uses, we analysed the roots of herbaceous plants using strain isolation, polymerase chain reaction (PCR), transmission electron microscopy (TEM) and co-cultivation experiments. A new sebacinoid strain named Serendipita herbamans belonging to Sebacinales group B was isolated from the roots of Bistorta vivipara, which is characterized by colourless monilioid cells (chlamydospores) that become yellow with age. This species was very common and widely distributed in association with a broad spectrum of herbaceous plant families in diverse habitats, independent of land use type. Ultrastructurally, the presence of S. herbamans was detected in the cortical cells of Plantago media, Potentilla anserina and Triticum aestivum. In addition, 13 few frequent molecular operational taxonomic units (MOTUs) or species were found across agricultural and grassland habitats, which did not exhibit a distinctive phylogenetic structure. Laboratory-based assays indicate that S. herbamans has the ability to colonize fine roots and stimulate plant growth. Although endophytic Sebacinales are widely distributed across agricultural and grassland habitats, TEM and nested PCR analyses reinforce the observation that these microorganisms are present in low quantity in plant roots, with no evidence of host specificity.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0094676</identifier><identifier>PMID: 24743185</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Agricultural ecosystems ; Agricultural land ; Agricultural management ; Agriculture ; Arabidopsis - microbiology ; Arabidopsis thaliana ; Basidiomycota - genetics ; Basidiomycota - physiology ; Bioinformatics ; Biology and Life Sciences ; Chlamydospores ; Community structure ; Cortex ; Cultivation ; Culture media ; Drinking water ; Ecology ; Ecology and Environmental Sciences ; Ecosystems ; Electron microscopy ; Endophytes ; Endophytes - genetics ; Endophytes - physiology ; Evolution ; Experiments ; Flowers & plants ; Fungi ; Fungi, Phytopathogenic ; Genetic aspects ; Genetic Variation ; Grassland ; Grasslands ; Habitats ; Haplotypes ; Host plants ; Host specificity ; Land use ; Microorganisms ; New species ; Panicum virgatum ; Phylogenetics ; Phylogeny ; Physiological aspects ; Piriformospora indica ; Plant cells ; Plant growth ; Plant roots ; Plant Roots - microbiology ; Poa - microbiology ; Polymerase chain reaction ; Population ; Roots ; Statistical analysis ; Transmission electron microscopy ; Triticum aestivum ; Vegetation ; Wheat</subject><ispartof>PloS one, 2014-04, Vol.9 (4), p.e94676-e94676</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Riess et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Riess et al 2014 Riess et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-dc95bf8405a53d0ba83aac4f29f598564cb028de19e11784ef03bcbcd27194503</citedby><cites>FETCH-LOGICAL-c692t-dc95bf8405a53d0ba83aac4f29f598564cb028de19e11784ef03bcbcd27194503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990532/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3990532/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24743185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Berg, Gabriele</contributor><creatorcontrib>Riess, Kai</creatorcontrib><creatorcontrib>Oberwinkler, Franz</creatorcontrib><creatorcontrib>Bauer, Robert</creatorcontrib><creatorcontrib>Garnica, Sigisfredo</creatorcontrib><title>Communities of endophytic sebacinales associated with roots of herbaceous plants in agricultural and grassland ecosystems are dominated by Serendipita herbamans sp. nov</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Endophytic fungi are known to be commonly associated with herbaceous plants, however, there are few studies focusing on their occurrence and distribution in plant roots from ecosystems with different land uses. To explore the phylogenetic diversity and community structure of Sebacinales endophytes from agricultural and grassland habitats under different land uses, we analysed the roots of herbaceous plants using strain isolation, polymerase chain reaction (PCR), transmission electron microscopy (TEM) and co-cultivation experiments. A new sebacinoid strain named Serendipita herbamans belonging to Sebacinales group B was isolated from the roots of Bistorta vivipara, which is characterized by colourless monilioid cells (chlamydospores) that become yellow with age. This species was very common and widely distributed in association with a broad spectrum of herbaceous plant families in diverse habitats, independent of land use type. Ultrastructurally, the presence of S. herbamans was detected in the cortical cells of Plantago media, Potentilla anserina and Triticum aestivum. In addition, 13 few frequent molecular operational taxonomic units (MOTUs) or species were found across agricultural and grassland habitats, which did not exhibit a distinctive phylogenetic structure. Laboratory-based assays indicate that S. herbamans has the ability to colonize fine roots and stimulate plant growth. Although endophytic Sebacinales are widely distributed across agricultural and grassland habitats, TEM and nested PCR analyses reinforce the observation that these microorganisms are present in low quantity in plant roots, with no evidence of host specificity.</description><subject>Agricultural ecosystems</subject><subject>Agricultural land</subject><subject>Agricultural management</subject><subject>Agriculture</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis thaliana</subject><subject>Basidiomycota - genetics</subject><subject>Basidiomycota - physiology</subject><subject>Bioinformatics</subject><subject>Biology and Life Sciences</subject><subject>Chlamydospores</subject><subject>Community structure</subject><subject>Cortex</subject><subject>Cultivation</subject><subject>Culture media</subject><subject>Drinking water</subject><subject>Ecology</subject><subject>Ecology and Environmental Sciences</subject><subject>Ecosystems</subject><subject>Electron microscopy</subject><subject>Endophytes</subject><subject>Endophytes - genetics</subject><subject>Endophytes - physiology</subject><subject>Evolution</subject><subject>Experiments</subject><subject>Flowers & plants</subject><subject>Fungi</subject><subject>Fungi, Phytopathogenic</subject><subject>Genetic aspects</subject><subject>Genetic Variation</subject><subject>Grassland</subject><subject>Grasslands</subject><subject>Habitats</subject><subject>Haplotypes</subject><subject>Host plants</subject><subject>Host specificity</subject><subject>Land use</subject><subject>Microorganisms</subject><subject>New species</subject><subject>Panicum virgatum</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Piriformospora indica</subject><subject>Plant cells</subject><subject>Plant growth</subject><subject>Plant roots</subject><subject>Plant Roots - microbiology</subject><subject>Poa - microbiology</subject><subject>Polymerase chain reaction</subject><subject>Population</subject><subject>Roots</subject><subject>Statistical analysis</subject><subject>Transmission electron microscopy</subject><subject>Triticum aestivum</subject><subject>Vegetation</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9-K1DAUxoso7rr6BqIBQfRixqRJ2vRGWBb_DCwsuOptSJO0zdAm3SRdnTfyMU1nZpcZ2QvJRcPJ73wn_U5Olr1EcIlwiT6s3eSt6Jejs3oJYUWKsniUnaIK54sih_jxwf4kexbCGkKKWVE8zU5yUhKMGD3N_ly4YZisiUYH4BqgrXJjt4lGgqBrIU0qkU5ECE4aEbUCv0zsgHcubvlO-0RpNwUw9sKmoLFAtN7IqY-TFz0QVoHWJ4F-3mnpwiZEPSRNr4FyQ6owy9YbcK19Km9GE8VOdxA2gDAugXW3z7MnjeiDfrH_nmU_Pn_6fvF1cXn1ZXVxfrmQRZXHhZIVrRtGIBUUK1gLhoWQpMmrhlaMFkTWMGdKo0ojVDKiG4hrWUuVl6giFOKz7PVOd-xd4HuTA0cUlYRQhPNErHaEcmLNR28G4TfcCcO3AedbLnwysNccIUEIq6tSEEUKigVSDZQ1kYpIxPImaX3cV5vqQSupbUyeHYken1jT8dbdclxVqZvzZd7tBby7mXSIfDBB6j6ZPTdle29WMsjKhL75B3347_ZUmxrPjW1cqitnUX6OS0oZZrhK1PIBKi2lByPTi2xMih8lvD9KSEzUv2MrphD46vrb_7NXP4_Ztwdsp0Ufu-D6KRpnwzFIdqD0LgSvm3uTEeTzQN25weeB4vuBSmmvDht0n3Q3QfgvQucfsw</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Riess, Kai</creator><creator>Oberwinkler, Franz</creator><creator>Bauer, Robert</creator><creator>Garnica, Sigisfredo</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140401</creationdate><title>Communities of endophytic sebacinales associated with roots of herbaceous plants in agricultural and grassland ecosystems are dominated by Serendipita herbamans sp. nov</title><author>Riess, Kai ; Oberwinkler, Franz ; Bauer, Robert ; Garnica, Sigisfredo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-dc95bf8405a53d0ba83aac4f29f598564cb028de19e11784ef03bcbcd27194503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Agricultural ecosystems</topic><topic>Agricultural land</topic><topic>Agricultural management</topic><topic>Agriculture</topic><topic>Arabidopsis - microbiology</topic><topic>Arabidopsis thaliana</topic><topic>Basidiomycota - genetics</topic><topic>Basidiomycota - physiology</topic><topic>Bioinformatics</topic><topic>Biology and Life Sciences</topic><topic>Chlamydospores</topic><topic>Community structure</topic><topic>Cortex</topic><topic>Cultivation</topic><topic>Culture media</topic><topic>Drinking water</topic><topic>Ecology</topic><topic>Ecology and Environmental Sciences</topic><topic>Ecosystems</topic><topic>Electron microscopy</topic><topic>Endophytes</topic><topic>Endophytes - genetics</topic><topic>Endophytes - physiology</topic><topic>Evolution</topic><topic>Experiments</topic><topic>Flowers & plants</topic><topic>Fungi</topic><topic>Fungi, Phytopathogenic</topic><topic>Genetic aspects</topic><topic>Genetic Variation</topic><topic>Grassland</topic><topic>Grasslands</topic><topic>Habitats</topic><topic>Haplotypes</topic><topic>Host plants</topic><topic>Host specificity</topic><topic>Land use</topic><topic>Microorganisms</topic><topic>New species</topic><topic>Panicum virgatum</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Physiological aspects</topic><topic>Piriformospora indica</topic><topic>Plant cells</topic><topic>Plant growth</topic><topic>Plant roots</topic><topic>Plant Roots - microbiology</topic><topic>Poa - microbiology</topic><topic>Polymerase chain reaction</topic><topic>Population</topic><topic>Roots</topic><topic>Statistical analysis</topic><topic>Transmission electron microscopy</topic><topic>Triticum aestivum</topic><topic>Vegetation</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Riess, Kai</creatorcontrib><creatorcontrib>Oberwinkler, Franz</creatorcontrib><creatorcontrib>Bauer, Robert</creatorcontrib><creatorcontrib>Garnica, Sigisfredo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Riess, Kai</au><au>Oberwinkler, Franz</au><au>Bauer, Robert</au><au>Garnica, Sigisfredo</au><au>Berg, Gabriele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Communities of endophytic sebacinales associated with roots of herbaceous plants in agricultural and grassland ecosystems are dominated by Serendipita herbamans sp. nov</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>9</volume><issue>4</issue><spage>e94676</spage><epage>e94676</epage><pages>e94676-e94676</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Endophytic fungi are known to be commonly associated with herbaceous plants, however, there are few studies focusing on their occurrence and distribution in plant roots from ecosystems with different land uses. To explore the phylogenetic diversity and community structure of Sebacinales endophytes from agricultural and grassland habitats under different land uses, we analysed the roots of herbaceous plants using strain isolation, polymerase chain reaction (PCR), transmission electron microscopy (TEM) and co-cultivation experiments. A new sebacinoid strain named Serendipita herbamans belonging to Sebacinales group B was isolated from the roots of Bistorta vivipara, which is characterized by colourless monilioid cells (chlamydospores) that become yellow with age. This species was very common and widely distributed in association with a broad spectrum of herbaceous plant families in diverse habitats, independent of land use type. Ultrastructurally, the presence of S. herbamans was detected in the cortical cells of Plantago media, Potentilla anserina and Triticum aestivum. In addition, 13 few frequent molecular operational taxonomic units (MOTUs) or species were found across agricultural and grassland habitats, which did not exhibit a distinctive phylogenetic structure. Laboratory-based assays indicate that S. herbamans has the ability to colonize fine roots and stimulate plant growth. Although endophytic Sebacinales are widely distributed across agricultural and grassland habitats, TEM and nested PCR analyses reinforce the observation that these microorganisms are present in low quantity in plant roots, with no evidence of host specificity.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24743185</pmid><doi>10.1371/journal.pone.0094676</doi><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2014-04, Vol.9 (4), p.e94676-e94676 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_1517445132 |
source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Agricultural ecosystems Agricultural land Agricultural management Agriculture Arabidopsis - microbiology Arabidopsis thaliana Basidiomycota - genetics Basidiomycota - physiology Bioinformatics Biology and Life Sciences Chlamydospores Community structure Cortex Cultivation Culture media Drinking water Ecology Ecology and Environmental Sciences Ecosystems Electron microscopy Endophytes Endophytes - genetics Endophytes - physiology Evolution Experiments Flowers & plants Fungi Fungi, Phytopathogenic Genetic aspects Genetic Variation Grassland Grasslands Habitats Haplotypes Host plants Host specificity Land use Microorganisms New species Panicum virgatum Phylogenetics Phylogeny Physiological aspects Piriformospora indica Plant cells Plant growth Plant roots Plant Roots - microbiology Poa - microbiology Polymerase chain reaction Population Roots Statistical analysis Transmission electron microscopy Triticum aestivum Vegetation Wheat |
title | Communities of endophytic sebacinales associated with roots of herbaceous plants in agricultural and grassland ecosystems are dominated by Serendipita herbamans sp. nov |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T11%3A14%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Communities%20of%20endophytic%20sebacinales%20associated%20with%20roots%20of%20herbaceous%20plants%20in%20agricultural%20and%20grassland%20ecosystems%20are%20dominated%20by%20Serendipita%20herbamans%20sp.%20nov&rft.jtitle=PloS%20one&rft.au=Riess,%20Kai&rft.date=2014-04-01&rft.volume=9&rft.issue=4&rft.spage=e94676&rft.epage=e94676&rft.pages=e94676-e94676&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0094676&rft_dat=%3Cgale_plos_%3EA375583839%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1517445132&rft_id=info:pmid/24743185&rft_galeid=A375583839&rft_doaj_id=oai_doaj_org_article_11a448b97a4d4653a1df0cb4cd4c182f&rfr_iscdi=true |