Embryonic protein undernutrition by albumen removal programs the hepatic amino acid and glucose metabolism during the perinatal period in an avian model
Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in th...
Gespeichert in:
| Veröffentlicht in: | PloS one 2014-04, Vol.9 (4), p.e94902-e94902 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e94902 |
|---|---|
| container_issue | 4 |
| container_start_page | e94902 |
| container_title | PloS one |
| container_volume | 9 |
| creator | Willems, Els Hu, Tjing-Tjing Soler Vasco, Laura Buyse, Johan Decuypere, Eddy Arckens, Lutgarde Everaert, Nadia |
| description | Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in the egg. Therefore, in the chicken, the direct effects of protein deficiency by albumen removal early during incubation can be examined. Prenatal protein undernutrition was established in layer-type eggs by the partial replacement of albumen by saline at embryonic day 1 (albumen-deprived group), compared to a mock-treated sham and a non-treated control group. At hatch, survival of the albumen-deprived group was lower compared to the control and sham group due to increased early mortality by the manipulation. No treatment differences in yolk-free body weight or yolk weight could be detected. The water content of the yolk was reduced, whereas the water content of the carcass was increased in the albumen-deprived group, compared to the control group, indicating less uptake of nutrients from the yolk. At embryonic day 16, 20 and at hatch, plasma triiodothyronine (T3), corticosterone, lactate or glucose concentrations and hepatic glycogen content were not affected by treatment. At embryonic day 20, the plasma thyroxine (T4) concentrations of the albumen-deprived embryos was reduced compared to the control group, indicating a decreased metabolic rate. Screening for differential protein expression in the liver at hatch using two-dimensional difference gel electrophoresis revealed not only changed abundance of proteins important for amino acid metabolism, but also of enzymes related to energy and glucose metabolism. Interestingly, GLUT1, a glucose transporter, and PCK2 and FBP1, two out of three regulatory enzymes of the gluconeogenesis were dysregulated. No parallel differences in gene expressions causing the differences in protein abundance could be detected pointing to post-transcriptional or post-translational regulation of the observed differences. |
| doi_str_mv | 10.1371/journal.pone.0094902 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1516954678</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A375584092</galeid><doaj_id>oai_doaj_org_article_71a46765c4ac4358b56eee13546c1ab9</doaj_id><sourcerecordid>A375584092</sourcerecordid><originalsourceid>FETCH-LOGICAL-c770t-e53b30bb0b15bd14b6d9083bc1a652d000372a09bcc88c53e6b2b56d1a102c453</originalsourceid><addsrcrecordid>eNqNk9tq3DAQhk1padK0b1BaQ6E0F7vVwZLtm0IIaRMIBHq6FTrMehVkaSPZS_MmfdzKu5uQDbkoNvYw_v5_xoOmKN5iNMe0xp-vwxi9dPNV8DBHqK1aRJ4Vh7ilZMYJos8fxAfFq5SuEWK04fxlcUCqukKE88Pi71mv4m3wVperGAawvhy9gejHIdrBBl-q21I6Nfbgywh9WEs3kV2UfSqHJZRLWMkhy2VvfSiltqaU3pSdG3VIUPYwSBWcTX1pxmh9txGtIIdymLxyFEyZ68p8r21-9sGAe128WEiX4M3ufVT8-nr28_R8dnn17eL05HKm6xoNM2BUUaQUUpgpgyvFTYsaqjSWnBGDEKI1kahVWjeNZhS4IopxgyVGRFeMHhXvt74rF5LYDTUJzDBvWcXrJhMXW8IEeS1W0fYy3oogrdgkQuyEjHkCDkSNZZZwpiupK8qaXAkAMM1GuSHVZq8vu2qj6sFo8EOUbs90_4u3S9GFtaBt09JNu3Rr4Cx0kIsrK9ZkI9zEo8vdaKFAEMIbgTmvq6ns8Va1fFTs_ORSTDmEOWJ1xdc4s592LcZwM0IaRG-TBuekhzBuJlM3DWqayfbDI_Tp-e2oTuYRWb8I-c_0ZCpOaM1YU6GWZGr-BJUvA73V-YwvbM7vCY73BJkZ4M_QyTElcfHj-_-zV7_32Y8P2CVINyxTcOO0DGkfrLagjiGlCIv7yWIkphW9m4aYVlTsVjTL3j08Aveiu52k_wD-iDeM</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1516954678</pqid></control><display><type>article</type><title>Embryonic protein undernutrition by albumen removal programs the hepatic amino acid and glucose metabolism during the perinatal period in an avian model</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Willems, Els ; Hu, Tjing-Tjing ; Soler Vasco, Laura ; Buyse, Johan ; Decuypere, Eddy ; Arckens, Lutgarde ; Everaert, Nadia</creator><contributor>te Pas, Marinus F.W.</contributor><creatorcontrib>Willems, Els ; Hu, Tjing-Tjing ; Soler Vasco, Laura ; Buyse, Johan ; Decuypere, Eddy ; Arckens, Lutgarde ; Everaert, Nadia ; te Pas, Marinus F.W.</creatorcontrib><description>Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in the egg. Therefore, in the chicken, the direct effects of protein deficiency by albumen removal early during incubation can be examined. Prenatal protein undernutrition was established in layer-type eggs by the partial replacement of albumen by saline at embryonic day 1 (albumen-deprived group), compared to a mock-treated sham and a non-treated control group. At hatch, survival of the albumen-deprived group was lower compared to the control and sham group due to increased early mortality by the manipulation. No treatment differences in yolk-free body weight or yolk weight could be detected. The water content of the yolk was reduced, whereas the water content of the carcass was increased in the albumen-deprived group, compared to the control group, indicating less uptake of nutrients from the yolk. At embryonic day 16, 20 and at hatch, plasma triiodothyronine (T3), corticosterone, lactate or glucose concentrations and hepatic glycogen content were not affected by treatment. At embryonic day 20, the plasma thyroxine (T4) concentrations of the albumen-deprived embryos was reduced compared to the control group, indicating a decreased metabolic rate. Screening for differential protein expression in the liver at hatch using two-dimensional difference gel electrophoresis revealed not only changed abundance of proteins important for amino acid metabolism, but also of enzymes related to energy and glucose metabolism. Interestingly, GLUT1, a glucose transporter, and PCK2 and FBP1, two out of three regulatory enzymes of the gluconeogenesis were dysregulated. No parallel differences in gene expressions causing the differences in protein abundance could be detected pointing to post-transcriptional or post-translational regulation of the observed differences.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0094902</identifier><identifier>PMID: 24740266</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abundance ; Acids ; Albumen ; Albumins - deficiency ; Albumins - pharmacology ; Amino acids ; Amino Acids - metabolism ; Animal models ; Animal production & animal husbandry ; Animals ; Animals, Newborn ; Avian Proteins - genetics ; Avian Proteins - metabolism ; Biology and Life Sciences ; Body weight ; Chick Embryo ; Chickens ; Corticosterone ; Dehydrogenases ; Diet ; Disease Models, Animal ; Eggs ; Eggs - analysis ; Electrophoresis, Gel, Two-Dimensional ; Embryo ; Embryos ; Endocrinology ; Energy metabolism ; Enzymes ; Female ; Fetuses ; Fructose-Bisphosphatase - genetics ; Fructose-Bisphosphatase - metabolism ; Gel electrophoresis ; Gene expression ; Gene Expression Regulation, Developmental ; Gene regulation ; Gluconeogenesis ; Glucose ; Glucose - metabolism ; Glucose transporter ; Glucose Transporter Type 1 - genetics ; Glucose Transporter Type 1 - metabolism ; Glycogen ; Glycogen - metabolism ; Health aspects ; Hormonal effects ; Humans ; Hypotheses ; Incubation ; Infertility ; Insulin resistance ; Kwashiorkor - embryology ; Kwashiorkor - genetics ; Kwashiorkor - metabolism ; Laboratories ; Lactic acid ; Life Sciences ; Liver ; Liver - drug effects ; Liver - embryology ; Liver - metabolism ; Livestock ; Malnutrition ; Metabolic rate ; Metabolism ; Metabolites ; Moisture content ; Mortality ; Nutrient uptake ; Nutrients ; Nutrition ; Phosphoenolpyruvate Carboxykinase (ATP) - genetics ; Phosphoenolpyruvate Carboxykinase (ATP) - metabolism ; Physiological aspects ; Physiology ; Post-transcription ; Post-translation ; Poultry ; Poultry Diseases - embryology ; Poultry Diseases - genetics ; Poultry Diseases - metabolism ; Productions animales & zootechnie ; Protein deficiency ; Proteins ; Research and Analysis Methods ; Reverse Transcriptase Polymerase Chain Reaction ; Rodents ; Sciences du vivant ; Studies ; Survival Analysis ; Tandem Mass Spectrometry ; Thyroid hormones ; Thyroxine ; Thyroxine - blood ; Undernutrition ; Water content ; Weight reduction ; Yolk</subject><ispartof>PloS one, 2014-04, Vol.9 (4), p.e94902-e94902</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Willems et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>2014 Willems et al 2014 Willems et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c770t-e53b30bb0b15bd14b6d9083bc1a652d000372a09bcc88c53e6b2b56d1a102c453</citedby><cites>FETCH-LOGICAL-c770t-e53b30bb0b15bd14b6d9083bc1a652d000372a09bcc88c53e6b2b56d1a102c453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989345/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3989345/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,2098,2917,23853,27911,27912,53778,53780,79355,79356</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24740266$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01605746$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>te Pas, Marinus F.W.</contributor><creatorcontrib>Willems, Els</creatorcontrib><creatorcontrib>Hu, Tjing-Tjing</creatorcontrib><creatorcontrib>Soler Vasco, Laura</creatorcontrib><creatorcontrib>Buyse, Johan</creatorcontrib><creatorcontrib>Decuypere, Eddy</creatorcontrib><creatorcontrib>Arckens, Lutgarde</creatorcontrib><creatorcontrib>Everaert, Nadia</creatorcontrib><title>Embryonic protein undernutrition by albumen removal programs the hepatic amino acid and glucose metabolism during the perinatal period in an avian model</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in the egg. Therefore, in the chicken, the direct effects of protein deficiency by albumen removal early during incubation can be examined. Prenatal protein undernutrition was established in layer-type eggs by the partial replacement of albumen by saline at embryonic day 1 (albumen-deprived group), compared to a mock-treated sham and a non-treated control group. At hatch, survival of the albumen-deprived group was lower compared to the control and sham group due to increased early mortality by the manipulation. No treatment differences in yolk-free body weight or yolk weight could be detected. The water content of the yolk was reduced, whereas the water content of the carcass was increased in the albumen-deprived group, compared to the control group, indicating less uptake of nutrients from the yolk. At embryonic day 16, 20 and at hatch, plasma triiodothyronine (T3), corticosterone, lactate or glucose concentrations and hepatic glycogen content were not affected by treatment. At embryonic day 20, the plasma thyroxine (T4) concentrations of the albumen-deprived embryos was reduced compared to the control group, indicating a decreased metabolic rate. Screening for differential protein expression in the liver at hatch using two-dimensional difference gel electrophoresis revealed not only changed abundance of proteins important for amino acid metabolism, but also of enzymes related to energy and glucose metabolism. Interestingly, GLUT1, a glucose transporter, and PCK2 and FBP1, two out of three regulatory enzymes of the gluconeogenesis were dysregulated. No parallel differences in gene expressions causing the differences in protein abundance could be detected pointing to post-transcriptional or post-translational regulation of the observed differences.</description><subject>Abundance</subject><subject>Acids</subject><subject>Albumen</subject><subject>Albumins - deficiency</subject><subject>Albumins - pharmacology</subject><subject>Amino acids</subject><subject>Amino Acids - metabolism</subject><subject>Animal models</subject><subject>Animal production & animal husbandry</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Avian Proteins - genetics</subject><subject>Avian Proteins - metabolism</subject><subject>Biology and Life Sciences</subject><subject>Body weight</subject><subject>Chick Embryo</subject><subject>Chickens</subject><subject>Corticosterone</subject><subject>Dehydrogenases</subject><subject>Diet</subject><subject>Disease Models, Animal</subject><subject>Eggs</subject><subject>Eggs - analysis</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Embryo</subject><subject>Embryos</subject><subject>Endocrinology</subject><subject>Energy metabolism</subject><subject>Enzymes</subject><subject>Female</subject><subject>Fetuses</subject><subject>Fructose-Bisphosphatase - genetics</subject><subject>Fructose-Bisphosphatase - metabolism</subject><subject>Gel electrophoresis</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene regulation</subject><subject>Gluconeogenesis</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Glucose transporter</subject><subject>Glucose Transporter Type 1 - genetics</subject><subject>Glucose Transporter Type 1 - metabolism</subject><subject>Glycogen</subject><subject>Glycogen - metabolism</subject><subject>Health aspects</subject><subject>Hormonal effects</subject><subject>Humans</subject><subject>Hypotheses</subject><subject>Incubation</subject><subject>Infertility</subject><subject>Insulin resistance</subject><subject>Kwashiorkor - embryology</subject><subject>Kwashiorkor - genetics</subject><subject>Kwashiorkor - metabolism</subject><subject>Laboratories</subject><subject>Lactic acid</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - embryology</subject><subject>Liver - metabolism</subject><subject>Livestock</subject><subject>Malnutrition</subject><subject>Metabolic rate</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Moisture content</subject><subject>Mortality</subject><subject>Nutrient uptake</subject><subject>Nutrients</subject><subject>Nutrition</subject><subject>Phosphoenolpyruvate Carboxykinase (ATP) - genetics</subject><subject>Phosphoenolpyruvate Carboxykinase (ATP) - metabolism</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Post-transcription</subject><subject>Post-translation</subject><subject>Poultry</subject><subject>Poultry Diseases - embryology</subject><subject>Poultry Diseases - genetics</subject><subject>Poultry Diseases - metabolism</subject><subject>Productions animales & zootechnie</subject><subject>Protein deficiency</subject><subject>Proteins</subject><subject>Research and Analysis Methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rodents</subject><subject>Sciences du vivant</subject><subject>Studies</subject><subject>Survival Analysis</subject><subject>Tandem Mass Spectrometry</subject><subject>Thyroid hormones</subject><subject>Thyroxine</subject><subject>Thyroxine - blood</subject><subject>Undernutrition</subject><subject>Water content</subject><subject>Weight reduction</subject><subject>Yolk</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAQhk1padK0b1BaQ6E0F7vVwZLtm0IIaRMIBHq6FTrMehVkaSPZS_MmfdzKu5uQDbkoNvYw_v5_xoOmKN5iNMe0xp-vwxi9dPNV8DBHqK1aRJ4Vh7ilZMYJos8fxAfFq5SuEWK04fxlcUCqukKE88Pi71mv4m3wVperGAawvhy9gejHIdrBBl-q21I6Nfbgywh9WEs3kV2UfSqHJZRLWMkhy2VvfSiltqaU3pSdG3VIUPYwSBWcTX1pxmh9txGtIIdymLxyFEyZ68p8r21-9sGAe128WEiX4M3ufVT8-nr28_R8dnn17eL05HKm6xoNM2BUUaQUUpgpgyvFTYsaqjSWnBGDEKI1kahVWjeNZhS4IopxgyVGRFeMHhXvt74rF5LYDTUJzDBvWcXrJhMXW8IEeS1W0fYy3oogrdgkQuyEjHkCDkSNZZZwpiupK8qaXAkAMM1GuSHVZq8vu2qj6sFo8EOUbs90_4u3S9GFtaBt09JNu3Rr4Cx0kIsrK9ZkI9zEo8vdaKFAEMIbgTmvq6ns8Va1fFTs_ORSTDmEOWJ1xdc4s592LcZwM0IaRG-TBuekhzBuJlM3DWqayfbDI_Tp-e2oTuYRWb8I-c_0ZCpOaM1YU6GWZGr-BJUvA73V-YwvbM7vCY73BJkZ4M_QyTElcfHj-_-zV7_32Y8P2CVINyxTcOO0DGkfrLagjiGlCIv7yWIkphW9m4aYVlTsVjTL3j08Aveiu52k_wD-iDeM</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Willems, Els</creator><creator>Hu, Tjing-Tjing</creator><creator>Soler Vasco, Laura</creator><creator>Buyse, Johan</creator><creator>Decuypere, Eddy</creator><creator>Arckens, Lutgarde</creator><creator>Everaert, Nadia</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>Q33</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140401</creationdate><title>Embryonic protein undernutrition by albumen removal programs the hepatic amino acid and glucose metabolism during the perinatal period in an avian model</title><author>Willems, Els ; Hu, Tjing-Tjing ; Soler Vasco, Laura ; Buyse, Johan ; Decuypere, Eddy ; Arckens, Lutgarde ; Everaert, Nadia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c770t-e53b30bb0b15bd14b6d9083bc1a652d000372a09bcc88c53e6b2b56d1a102c453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Abundance</topic><topic>Acids</topic><topic>Albumen</topic><topic>Albumins - deficiency</topic><topic>Albumins - pharmacology</topic><topic>Amino acids</topic><topic>Amino Acids - metabolism</topic><topic>Animal models</topic><topic>Animal production & animal husbandry</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Avian Proteins - genetics</topic><topic>Avian Proteins - metabolism</topic><topic>Biology and Life Sciences</topic><topic>Body weight</topic><topic>Chick Embryo</topic><topic>Chickens</topic><topic>Corticosterone</topic><topic>Dehydrogenases</topic><topic>Diet</topic><topic>Disease Models, Animal</topic><topic>Eggs</topic><topic>Eggs - analysis</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Embryo</topic><topic>Embryos</topic><topic>Endocrinology</topic><topic>Energy metabolism</topic><topic>Enzymes</topic><topic>Female</topic><topic>Fetuses</topic><topic>Fructose-Bisphosphatase - genetics</topic><topic>Fructose-Bisphosphatase - metabolism</topic><topic>Gel electrophoresis</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene regulation</topic><topic>Gluconeogenesis</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Glucose transporter</topic><topic>Glucose Transporter Type 1 - genetics</topic><topic>Glucose Transporter Type 1 - metabolism</topic><topic>Glycogen</topic><topic>Glycogen - metabolism</topic><topic>Health aspects</topic><topic>Hormonal effects</topic><topic>Humans</topic><topic>Hypotheses</topic><topic>Incubation</topic><topic>Infertility</topic><topic>Insulin resistance</topic><topic>Kwashiorkor - embryology</topic><topic>Kwashiorkor - genetics</topic><topic>Kwashiorkor - metabolism</topic><topic>Laboratories</topic><topic>Lactic acid</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Liver - drug effects</topic><topic>Liver - embryology</topic><topic>Liver - metabolism</topic><topic>Livestock</topic><topic>Malnutrition</topic><topic>Metabolic rate</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Moisture content</topic><topic>Mortality</topic><topic>Nutrient uptake</topic><topic>Nutrients</topic><topic>Nutrition</topic><topic>Phosphoenolpyruvate Carboxykinase (ATP) - genetics</topic><topic>Phosphoenolpyruvate Carboxykinase (ATP) - metabolism</topic><topic>Physiological aspects</topic><topic>Physiology</topic><topic>Post-transcription</topic><topic>Post-translation</topic><topic>Poultry</topic><topic>Poultry Diseases - embryology</topic><topic>Poultry Diseases - genetics</topic><topic>Poultry Diseases - metabolism</topic><topic>Productions animales & zootechnie</topic><topic>Protein deficiency</topic><topic>Proteins</topic><topic>Research and Analysis Methods</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rodents</topic><topic>Sciences du vivant</topic><topic>Studies</topic><topic>Survival Analysis</topic><topic>Tandem Mass Spectrometry</topic><topic>Thyroid hormones</topic><topic>Thyroxine</topic><topic>Thyroxine - blood</topic><topic>Undernutrition</topic><topic>Water content</topic><topic>Weight reduction</topic><topic>Yolk</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Willems, Els</creatorcontrib><creatorcontrib>Hu, Tjing-Tjing</creatorcontrib><creatorcontrib>Soler Vasco, Laura</creatorcontrib><creatorcontrib>Buyse, Johan</creatorcontrib><creatorcontrib>Decuypere, Eddy</creatorcontrib><creatorcontrib>Arckens, Lutgarde</creatorcontrib><creatorcontrib>Everaert, Nadia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>Université de Liège - Open Repository and Bibliography (ORBI)</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Willems, Els</au><au>Hu, Tjing-Tjing</au><au>Soler Vasco, Laura</au><au>Buyse, Johan</au><au>Decuypere, Eddy</au><au>Arckens, Lutgarde</au><au>Everaert, Nadia</au><au>te Pas, Marinus F.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Embryonic protein undernutrition by albumen removal programs the hepatic amino acid and glucose metabolism during the perinatal period in an avian model</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>9</volume><issue>4</issue><spage>e94902</spage><epage>e94902</epage><pages>e94902-e94902</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Different animal models have been used to study the effects of prenatal protein undernutrition and the mechanisms by which these occur. In mammals, the maternal diet is manipulated, exerting both direct nutritional and indirect hormonal effects. Chicken embryos develop independent from the hen in the egg. Therefore, in the chicken, the direct effects of protein deficiency by albumen removal early during incubation can be examined. Prenatal protein undernutrition was established in layer-type eggs by the partial replacement of albumen by saline at embryonic day 1 (albumen-deprived group), compared to a mock-treated sham and a non-treated control group. At hatch, survival of the albumen-deprived group was lower compared to the control and sham group due to increased early mortality by the manipulation. No treatment differences in yolk-free body weight or yolk weight could be detected. The water content of the yolk was reduced, whereas the water content of the carcass was increased in the albumen-deprived group, compared to the control group, indicating less uptake of nutrients from the yolk. At embryonic day 16, 20 and at hatch, plasma triiodothyronine (T3), corticosterone, lactate or glucose concentrations and hepatic glycogen content were not affected by treatment. At embryonic day 20, the plasma thyroxine (T4) concentrations of the albumen-deprived embryos was reduced compared to the control group, indicating a decreased metabolic rate. Screening for differential protein expression in the liver at hatch using two-dimensional difference gel electrophoresis revealed not only changed abundance of proteins important for amino acid metabolism, but also of enzymes related to energy and glucose metabolism. Interestingly, GLUT1, a glucose transporter, and PCK2 and FBP1, two out of three regulatory enzymes of the gluconeogenesis were dysregulated. No parallel differences in gene expressions causing the differences in protein abundance could be detected pointing to post-transcriptional or post-translational regulation of the observed differences.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24740266</pmid><doi>10.1371/journal.pone.0094902</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-04, Vol.9 (4), p.e94902-e94902 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1516954678 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Abundance Acids Albumen Albumins - deficiency Albumins - pharmacology Amino acids Amino Acids - metabolism Animal models Animal production & animal husbandry Animals Animals, Newborn Avian Proteins - genetics Avian Proteins - metabolism Biology and Life Sciences Body weight Chick Embryo Chickens Corticosterone Dehydrogenases Diet Disease Models, Animal Eggs Eggs - analysis Electrophoresis, Gel, Two-Dimensional Embryo Embryos Endocrinology Energy metabolism Enzymes Female Fetuses Fructose-Bisphosphatase - genetics Fructose-Bisphosphatase - metabolism Gel electrophoresis Gene expression Gene Expression Regulation, Developmental Gene regulation Gluconeogenesis Glucose Glucose - metabolism Glucose transporter Glucose Transporter Type 1 - genetics Glucose Transporter Type 1 - metabolism Glycogen Glycogen - metabolism Health aspects Hormonal effects Humans Hypotheses Incubation Infertility Insulin resistance Kwashiorkor - embryology Kwashiorkor - genetics Kwashiorkor - metabolism Laboratories Lactic acid Life Sciences Liver Liver - drug effects Liver - embryology Liver - metabolism Livestock Malnutrition Metabolic rate Metabolism Metabolites Moisture content Mortality Nutrient uptake Nutrients Nutrition Phosphoenolpyruvate Carboxykinase (ATP) - genetics Phosphoenolpyruvate Carboxykinase (ATP) - metabolism Physiological aspects Physiology Post-transcription Post-translation Poultry Poultry Diseases - embryology Poultry Diseases - genetics Poultry Diseases - metabolism Productions animales & zootechnie Protein deficiency Proteins Research and Analysis Methods Reverse Transcriptase Polymerase Chain Reaction Rodents Sciences du vivant Studies Survival Analysis Tandem Mass Spectrometry Thyroid hormones Thyroxine Thyroxine - blood Undernutrition Water content Weight reduction Yolk |
| title | Embryonic protein undernutrition by albumen removal programs the hepatic amino acid and glucose metabolism during the perinatal period in an avian model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T19%3A58%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Embryonic%20protein%20undernutrition%20by%20albumen%20removal%20programs%20the%20hepatic%20amino%20acid%20and%20glucose%20metabolism%20during%20the%20perinatal%20period%20in%20an%20avian%20model&rft.jtitle=PloS%20one&rft.au=Willems,%20Els&rft.date=2014-04-01&rft.volume=9&rft.issue=4&rft.spage=e94902&rft.epage=e94902&rft.pages=e94902-e94902&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0094902&rft_dat=%3Cgale_plos_%3EA375584092%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1516954678&rft_id=info:pmid/24740266&rft_galeid=A375584092&rft_doaj_id=oai_doaj_org_article_71a46765c4ac4358b56eee13546c1ab9&rfr_iscdi=true |