Heteroconium chaetospira induces resistance to clubroot via upregulation of host genes involved in jasmonic acid, ethylene, and auxin biosynthesis
An endophytic fungus, Heteroconium chaetospira isolate BC2HB1 (Hc), suppressed clubroot (Plasmodiophora brassicae -Pb) on canola in growth-cabinet trials. Confocal microscopy demonstrated that Hc penetrated canola roots and colonized cortical tissues. Based on qPCR analysis, the amount of Hc DNA fou...
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| Veröffentlicht in: | PloS one 2014-04, Vol.9 (4), p.e94144-e94144 |
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| creator | Lahlali, Rachid McGregor, Linda Song, Tao Gossen, Bruce D Narisawa, Kazuhiko Peng, Gary |
| description | An endophytic fungus, Heteroconium chaetospira isolate BC2HB1 (Hc), suppressed clubroot (Plasmodiophora brassicae -Pb) on canola in growth-cabinet trials. Confocal microscopy demonstrated that Hc penetrated canola roots and colonized cortical tissues. Based on qPCR analysis, the amount of Hc DNA found in canola roots at 14 days after treatment was negatively correlated (r = 0.92, P |
| doi_str_mv | 10.1371/journal.pone.0094144 |
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Confocal microscopy demonstrated that Hc penetrated canola roots and colonized cortical tissues. Based on qPCR analysis, the amount of Hc DNA found in canola roots at 14 days after treatment was negatively correlated (r = 0.92, P<0.001) with the severity of clubroot at 5 weeks after treatment at a low (2×10(5) spores pot(-1)) but not high (2×10(5) spores pot(-1)) dose of pathogen inoculum. Transcript levels of nine B. napus (Bn) genes in roots treated with Hc plus Pb, Pb alone and a nontreated control were analyzed using qPCR supplemented with biochemical analysis for the activity of phenylalanine ammonia lyases (PAL). These genes encode enzymes involved in several biosynthetic pathways related potentially to plant defence. Hc plus Pb increased the activity of PAL but not that of the other two genes (BnCCR and BnOPCL) involved also in phenylpropanoid biosynthesis, relative to Pb inoculation alone. In contrast, expression of several genes involved in the jasmonic acid (BnOPR2), ethylene (BnACO), auxin (BnAAO1), and PR-2 protein (BnPR-2) biosynthesis were upregulated by 63, 48, 3, and 3 fold, respectively, by Hc plus Pb over Pb alone. This indicates that these genes may be involved in inducing resistance in canola by Hc against clubroot. The upregulation of BnAAO1 appears to be related to both pathogenesis of clubroot and induced defence mechanisms in canola roots. This is the first report on regulation of specific host genes involved in induced plant resistance by a non-mycorrhizal endophyte.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0094144</identifier><identifier>PMID: 24714177</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acids ; Ammonia ; Analysis ; Barley ; Biochemical analysis ; Biology and Life Sciences ; Biosynthesis ; Brassica rapa ; Brassica rapa - genetics ; Brassica rapa - parasitology ; Canola ; Chemical properties ; Clubroot ; Confocal ; Confocal microscopy ; Cortex ; Cyclopentanes - metabolism ; Deoxyribonucleic acid ; Disease ; DNA ; Endophytes ; Ethylene ; Ethylenes - biosynthesis ; Flowers & plants ; Food ; Gene expression ; Gene regulation ; Genes ; Genetic aspects ; Heteroconium chaetospira ; Host plants ; Indoleacetic Acids - metabolism ; Inoculation ; Inoculum ; Jasmonic acid ; Lead ; Manufacturers ; Microscopy ; Morphology ; Mycorrhizae - genetics ; Myxomycetes ; Oxylipins - metabolism ; Pathogenesis ; Pesticides ; Phenylalanine ; Physiological aspects ; Plant Diseases - genetics ; Plant Diseases - parasitology ; Plant resistance ; Plasmodiophora brassicae ; Plasmodiophorida ; Protein biosynthesis ; Protein folding ; Proteins ; Protozoan Infections - genetics ; Roots ; Spores ; Tissues ; Transcription ; Transcriptional Activation ; Trends ; Up-Regulation</subject><ispartof>PloS one, 2014-04, Vol.9 (4), p.e94144-e94144</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Lahlali et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Lahlali et al 2014 Lahlali et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-49bd47b4093d46102ea4e6e1e1c763af203f118aa730093ceb3c44877a09696b3</citedby><cites>FETCH-LOGICAL-c758t-49bd47b4093d46102ea4e6e1e1c763af203f118aa730093ceb3c44877a09696b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979836/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979836/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24714177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wilson, Richard A.</contributor><creatorcontrib>Lahlali, Rachid</creatorcontrib><creatorcontrib>McGregor, Linda</creatorcontrib><creatorcontrib>Song, Tao</creatorcontrib><creatorcontrib>Gossen, Bruce D</creatorcontrib><creatorcontrib>Narisawa, Kazuhiko</creatorcontrib><creatorcontrib>Peng, Gary</creatorcontrib><title>Heteroconium chaetospira induces resistance to clubroot via upregulation of host genes involved in jasmonic acid, ethylene, and auxin biosynthesis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>An endophytic fungus, Heteroconium chaetospira isolate BC2HB1 (Hc), suppressed clubroot (Plasmodiophora brassicae -Pb) on canola in growth-cabinet trials. Confocal microscopy demonstrated that Hc penetrated canola roots and colonized cortical tissues. Based on qPCR analysis, the amount of Hc DNA found in canola roots at 14 days after treatment was negatively correlated (r = 0.92, P<0.001) with the severity of clubroot at 5 weeks after treatment at a low (2×10(5) spores pot(-1)) but not high (2×10(5) spores pot(-1)) dose of pathogen inoculum. Transcript levels of nine B. napus (Bn) genes in roots treated with Hc plus Pb, Pb alone and a nontreated control were analyzed using qPCR supplemented with biochemical analysis for the activity of phenylalanine ammonia lyases (PAL). These genes encode enzymes involved in several biosynthetic pathways related potentially to plant defence. Hc plus Pb increased the activity of PAL but not that of the other two genes (BnCCR and BnOPCL) involved also in phenylpropanoid biosynthesis, relative to Pb inoculation alone. In contrast, expression of several genes involved in the jasmonic acid (BnOPR2), ethylene (BnACO), auxin (BnAAO1), and PR-2 protein (BnPR-2) biosynthesis were upregulated by 63, 48, 3, and 3 fold, respectively, by Hc plus Pb over Pb alone. This indicates that these genes may be involved in inducing resistance in canola by Hc against clubroot. The upregulation of BnAAO1 appears to be related to both pathogenesis of clubroot and induced defence mechanisms in canola roots. 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metabolism</subject><subject>Inoculation</subject><subject>Inoculum</subject><subject>Jasmonic acid</subject><subject>Lead</subject><subject>Manufacturers</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Mycorrhizae - genetics</subject><subject>Myxomycetes</subject><subject>Oxylipins - metabolism</subject><subject>Pathogenesis</subject><subject>Pesticides</subject><subject>Phenylalanine</subject><subject>Physiological aspects</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - parasitology</subject><subject>Plant resistance</subject><subject>Plasmodiophora brassicae</subject><subject>Plasmodiophorida</subject><subject>Protein biosynthesis</subject><subject>Protein folding</subject><subject>Proteins</subject><subject>Protozoan Infections - genetics</subject><subject>Roots</subject><subject>Spores</subject><subject>Tissues</subject><subject>Transcription</subject><subject>Transcriptional Activation</subject><subject>Trends</subject><subject>Up-Regulation</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk99q2zAUxs3YWLtubzA2wWBs0GSWJVv2zaCUbS0UCvt3K47l41hBkVJJDs1r7ImnNGlJRi-GLiSk3_cdnSOdLHtN8yllgn6au9FbMNOlszjN84ZTzp9kx7RhxaQqcvZ0b32UvQhhnuclq6vqeXZUcEE5FeI4-3OBEb1TzupxQdQAGF1Yag9E225UGIjHoEMEq5BER5QZW-9cJCsNZFx6nI0GonaWuJ4MLkQyQ5tU2q6cWWGXFmQOYZH8FQGlu1OCcVibBJ0SsB2B8TYhrXZhbeOwifUye9aDCfhqN59kv75--Xl-Mbm6_nZ5fnY1UaKs44Q3bcdFy_OGdbyieYHAsUKKVImKQZ_S7imtAQRL1WEKW6Y4r4WAvKmaqmUn2dut79K4IHflDJKWlNUFL0qRiMst0TmYy6XXC_Br6UDLuw3nZxJ81MqgpAUt8rotmwZZ0hbAqwKbFLgriq7KWfL6vIs2tgvsFNrowRyYHp5YPciZW0nWiKZmVTL4sDPw7mbEEOVCB4XGgEU33t2bc8Z4euST7N0_6OPZ7agZpAS07V2Kqzam8oyJsqwZqzbU9BEqjQ4XOn0b7HXaPxB8PBAkJuJtnMEYgrz88f3_2evfh-z7PXZAMHEIzoybzxcOQb4FlXcheOwfikxzuWmd-2rITevIXesk2Zv9B3oQ3fcK-wuR7xUc</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Lahlali, Rachid</creator><creator>McGregor, Linda</creator><creator>Song, Tao</creator><creator>Gossen, Bruce D</creator><creator>Narisawa, Kazuhiko</creator><creator>Peng, Gary</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140401</creationdate><title>Heteroconium chaetospira induces resistance to clubroot via upregulation of host genes involved in jasmonic acid, ethylene, and auxin biosynthesis</title><author>Lahlali, Rachid ; McGregor, Linda ; Song, Tao ; Gossen, Bruce D ; Narisawa, Kazuhiko ; Peng, Gary</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-49bd47b4093d46102ea4e6e1e1c763af203f118aa730093ceb3c44877a09696b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acids</topic><topic>Ammonia</topic><topic>Analysis</topic><topic>Barley</topic><topic>Biochemical analysis</topic><topic>Biology and Life Sciences</topic><topic>Biosynthesis</topic><topic>Brassica rapa</topic><topic>Brassica rapa - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lahlali, Rachid</au><au>McGregor, Linda</au><au>Song, Tao</au><au>Gossen, Bruce D</au><au>Narisawa, Kazuhiko</au><au>Peng, Gary</au><au>Wilson, Richard A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heteroconium chaetospira induces resistance to clubroot via upregulation of host genes involved in jasmonic acid, ethylene, and auxin biosynthesis</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>9</volume><issue>4</issue><spage>e94144</spage><epage>e94144</epage><pages>e94144-e94144</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>An endophytic fungus, Heteroconium chaetospira isolate BC2HB1 (Hc), suppressed clubroot (Plasmodiophora brassicae -Pb) on canola in growth-cabinet trials. Confocal microscopy demonstrated that Hc penetrated canola roots and colonized cortical tissues. Based on qPCR analysis, the amount of Hc DNA found in canola roots at 14 days after treatment was negatively correlated (r = 0.92, P<0.001) with the severity of clubroot at 5 weeks after treatment at a low (2×10(5) spores pot(-1)) but not high (2×10(5) spores pot(-1)) dose of pathogen inoculum. Transcript levels of nine B. napus (Bn) genes in roots treated with Hc plus Pb, Pb alone and a nontreated control were analyzed using qPCR supplemented with biochemical analysis for the activity of phenylalanine ammonia lyases (PAL). These genes encode enzymes involved in several biosynthetic pathways related potentially to plant defence. Hc plus Pb increased the activity of PAL but not that of the other two genes (BnCCR and BnOPCL) involved also in phenylpropanoid biosynthesis, relative to Pb inoculation alone. In contrast, expression of several genes involved in the jasmonic acid (BnOPR2), ethylene (BnACO), auxin (BnAAO1), and PR-2 protein (BnPR-2) biosynthesis were upregulated by 63, 48, 3, and 3 fold, respectively, by Hc plus Pb over Pb alone. This indicates that these genes may be involved in inducing resistance in canola by Hc against clubroot. The upregulation of BnAAO1 appears to be related to both pathogenesis of clubroot and induced defence mechanisms in canola roots. This is the first report on regulation of specific host genes involved in induced plant resistance by a non-mycorrhizal endophyte.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24714177</pmid><doi>10.1371/journal.pone.0094144</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Ammonia Analysis Barley Biochemical analysis Biology and Life Sciences Biosynthesis Brassica rapa Brassica rapa - genetics Brassica rapa - parasitology Canola Chemical properties Clubroot Confocal Confocal microscopy Cortex Cyclopentanes - metabolism Deoxyribonucleic acid Disease DNA Endophytes Ethylene Ethylenes - biosynthesis Flowers & plants Food Gene expression Gene regulation Genes Genetic aspects Heteroconium chaetospira Host plants Indoleacetic Acids - metabolism Inoculation Inoculum Jasmonic acid Lead Manufacturers Microscopy Morphology Mycorrhizae - genetics Myxomycetes Oxylipins - metabolism Pathogenesis Pesticides Phenylalanine Physiological aspects Plant Diseases - genetics Plant Diseases - parasitology Plant resistance Plasmodiophora brassicae Plasmodiophorida Protein biosynthesis Protein folding Proteins Protozoan Infections - genetics Roots Spores Tissues Transcription Transcriptional Activation Trends Up-Regulation |
| title | Heteroconium chaetospira induces resistance to clubroot via upregulation of host genes involved in jasmonic acid, ethylene, and auxin biosynthesis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T14%3A32%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Heteroconium%20chaetospira%20induces%20resistance%20to%20clubroot%20via%20upregulation%20of%20host%20genes%20involved%20in%20jasmonic%20acid,%20ethylene,%20and%20auxin%20biosynthesis&rft.jtitle=PloS%20one&rft.au=Lahlali,%20Rachid&rft.date=2014-04-01&rft.volume=9&rft.issue=4&rft.spage=e94144&rft.epage=e94144&rft.pages=e94144-e94144&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0094144&rft_dat=%3Cgale_plos_%3EA375583367%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1513824257&rft_id=info:pmid/24714177&rft_galeid=A375583367&rft_doaj_id=oai_doaj_org_article_121208b599e34252a462e9009d22d603&rfr_iscdi=true |