Simultaneous measurement of thirteen steroid hormones in women with polycystic ovary syndrome and control women using liquid chromatography-tandem mass spectrometry
The measurement of adrenal and ovarian androgens in women with PCOS has been difficult based on poor specificity and sensitivity of assays in the female range. Women with PCOS (NIH criteria; n = 52) and control subjects with 25-35 day menstrual cycles, no evidence of hyperandrogenism and matched for...
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| creator | Keefe, Candace C Goldman, Mildred M Zhang, Ke Clarke, Nigel Reitz, Richard E Welt, Corrine K |
| description | The measurement of adrenal and ovarian androgens in women with PCOS has been difficult based on poor specificity and sensitivity of assays in the female range.
Women with PCOS (NIH criteria; n = 52) and control subjects with 25-35 day menstrual cycles, no evidence of hyperandrogenism and matched for BMI (n = 42) underwent morning blood sampling. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to simultaneously measure 13 steroids from a single blood sample to measure adrenal and ovarian steroids. Androgen and progesterone results were compared in the same samples using RIA.
Testosterone, androstenedione, progesterone and 17OH progesterone levels were higher when measured using RIA compared to LC-MS/MS, although the testosterone RIA demonstrated the best agreement with the LC-MS/MS using a Bland-Altman analysis. Results using LC-MS/MS demonstrated that the concentration of androgens and their precursors were higher in women with PCOS than controls [median (2.5, 97.5th %ile); 1607 (638, 3085) vs. 1143 (511, 4784) ng/dL; p = 0.03]. Women with PCOS had higher testosterone [49 (16, 125) vs. 24 (10, 59) ng/dL], androstenedione [203 (98, 476) vs. 106 (69, 223) ng/dL] and 17OH progesterone levels [80 (17, 176) vs. 44 (17, 142) ng/dL] compared to controls (all P |
| doi_str_mv | 10.1371/journal.pone.0093805 |
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Women with PCOS (NIH criteria; n = 52) and control subjects with 25-35 day menstrual cycles, no evidence of hyperandrogenism and matched for BMI (n = 42) underwent morning blood sampling. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to simultaneously measure 13 steroids from a single blood sample to measure adrenal and ovarian steroids. Androgen and progesterone results were compared in the same samples using RIA.
Testosterone, androstenedione, progesterone and 17OH progesterone levels were higher when measured using RIA compared to LC-MS/MS, although the testosterone RIA demonstrated the best agreement with the LC-MS/MS using a Bland-Altman analysis. Results using LC-MS/MS demonstrated that the concentration of androgens and their precursors were higher in women with PCOS than controls [median (2.5, 97.5th %ile); 1607 (638, 3085) vs. 1143 (511, 4784) ng/dL; p = 0.03]. Women with PCOS had higher testosterone [49 (16, 125) vs. 24 (10, 59) ng/dL], androstenedione [203 (98, 476) vs. 106 (69, 223) ng/dL] and 17OH progesterone levels [80 (17, 176) vs. 44 (17, 142) ng/dL] compared to controls (all P<0.02), but no differences in serum concentrations of the adrenal steroids DHEAS, cortisol, corticosterone and their 11 deoxy precursors. Women with PCOS also had an increase in the product:precursor ratio for 3β-hydroxysteroid dehydrogenase [22% (6, 92) vs. 20% (4, 43); p = 0.009].
LC-MS/MS was superior to RIA in measuring androstenedione, progesterone and 17OH progesterone levels, while testosterone measurements were better matched in the two assays. Androgen levels were higher in women with PCOS in the absence of a difference in adrenal-predominant steroids. These data support previous findings that the ovary is an important source for the androgen excess in women with PCOS.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0093805</identifier><identifier>PMID: 24713888</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>17-alpha-Hydroxyprogesterone - blood ; Accuracy ; Acne ; Adolescent ; Adult ; Androgens ; Androstenedione ; Androstenedione - blood ; Biology and Life Sciences ; Blood ; Body mass ; Body mass index ; Chromatography ; Chromatography, Liquid ; Corticosterone ; Cortisol ; Female ; Hormones ; Hormones - blood ; Humans ; Hydrocortisone ; Hyperandrogenism - blood ; Immunoassay ; Intubation ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Medicine and Health Sciences ; Menstruation ; Polycystic ovary syndrome ; Polycystic Ovary Syndrome - blood ; Progesterone ; Research and Analysis Methods ; Scientific imaging ; Spectroscopy ; Steroid hormones ; Steroids ; Steroids - blood ; Tandem Mass Spectrometry ; Testosterone ; Testosterone - blood ; Young Adult</subject><ispartof>PloS one, 2014-04, Vol.9 (4), p.e93805-e93805</ispartof><rights>2014 Keefe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Keefe et al 2014 Keefe et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-e676ec2ac1ce36ac988c0ad2418073da9664de48fa8dbdc3734d313a2792469b3</citedby><cites>FETCH-LOGICAL-c526t-e676ec2ac1ce36ac988c0ad2418073da9664de48fa8dbdc3734d313a2792469b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979722/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979722/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24713888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Wilson, Elizabeth</contributor><creatorcontrib>Keefe, Candace C</creatorcontrib><creatorcontrib>Goldman, Mildred M</creatorcontrib><creatorcontrib>Zhang, Ke</creatorcontrib><creatorcontrib>Clarke, Nigel</creatorcontrib><creatorcontrib>Reitz, Richard E</creatorcontrib><creatorcontrib>Welt, Corrine K</creatorcontrib><title>Simultaneous measurement of thirteen steroid hormones in women with polycystic ovary syndrome and control women using liquid chromatography-tandem mass spectrometry</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The measurement of adrenal and ovarian androgens in women with PCOS has been difficult based on poor specificity and sensitivity of assays in the female range.
Women with PCOS (NIH criteria; n = 52) and control subjects with 25-35 day menstrual cycles, no evidence of hyperandrogenism and matched for BMI (n = 42) underwent morning blood sampling. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to simultaneously measure 13 steroids from a single blood sample to measure adrenal and ovarian steroids. Androgen and progesterone results were compared in the same samples using RIA.
Testosterone, androstenedione, progesterone and 17OH progesterone levels were higher when measured using RIA compared to LC-MS/MS, although the testosterone RIA demonstrated the best agreement with the LC-MS/MS using a Bland-Altman analysis. Results using LC-MS/MS demonstrated that the concentration of androgens and their precursors were higher in women with PCOS than controls [median (2.5, 97.5th %ile); 1607 (638, 3085) vs. 1143 (511, 4784) ng/dL; p = 0.03]. Women with PCOS had higher testosterone [49 (16, 125) vs. 24 (10, 59) ng/dL], androstenedione [203 (98, 476) vs. 106 (69, 223) ng/dL] and 17OH progesterone levels [80 (17, 176) vs. 44 (17, 142) ng/dL] compared to controls (all P<0.02), but no differences in serum concentrations of the adrenal steroids DHEAS, cortisol, corticosterone and their 11 deoxy precursors. Women with PCOS also had an increase in the product:precursor ratio for 3β-hydroxysteroid dehydrogenase [22% (6, 92) vs. 20% (4, 43); p = 0.009].
LC-MS/MS was superior to RIA in measuring androstenedione, progesterone and 17OH progesterone levels, while testosterone measurements were better matched in the two assays. Androgen levels were higher in women with PCOS in the absence of a difference in adrenal-predominant steroids. These data support previous findings that the ovary is an important source for the androgen excess in women with PCOS.</description><subject>17-alpha-Hydroxyprogesterone - blood</subject><subject>Accuracy</subject><subject>Acne</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Androgens</subject><subject>Androstenedione</subject><subject>Androstenedione - blood</subject><subject>Biology and Life Sciences</subject><subject>Blood</subject><subject>Body mass</subject><subject>Body mass index</subject><subject>Chromatography</subject><subject>Chromatography, Liquid</subject><subject>Corticosterone</subject><subject>Cortisol</subject><subject>Female</subject><subject>Hormones</subject><subject>Hormones - blood</subject><subject>Humans</subject><subject>Hydrocortisone</subject><subject>Hyperandrogenism - blood</subject><subject>Immunoassay</subject><subject>Intubation</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicine and Health Sciences</subject><subject>Menstruation</subject><subject>Polycystic ovary syndrome</subject><subject>Polycystic Ovary Syndrome - blood</subject><subject>Progesterone</subject><subject>Research and Analysis Methods</subject><subject>Scientific imaging</subject><subject>Spectroscopy</subject><subject>Steroid hormones</subject><subject>Steroids</subject><subject>Steroids - blood</subject><subject>Tandem Mass Spectrometry</subject><subject>Testosterone</subject><subject>Testosterone - blood</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNptUl1rFDEUHUSxtfoPRAO--LJrvjaTeRFK8aNQ8EF9Dtnkzk6WzGSaZFrm__hDzXanpRUhkJCce-65J6eq3hK8Jqwmn_ZhioP26zEMsMa4YRJvnlWnpGF0JShmzx-dT6pXKe0x3jApxMvqhPKaMCnlafXnp-snn_UAYUqoB52mCD0MGYUW5c7FDDCglCEGZ1EXYl_aJeQGdBsKDN263KEx-NnMKTuDwo2OM0rzYGN5R3qwyIQhx-CXgim5YYe8u54Kn-kKSuewi3rs5lWRYaFHvU4JpRFMPnDkOL-uXrTaJ3iz7GfV769ffl18X139-HZ5cX61Mhsq8gpELcBQbYgBJrRppDRYW8qJxDWzuhGCW-Cy1dJurWE145YRpmndUC6aLTur3h95Rx-SWgxOimyKWQeWuiAujwgb9F6N0fVlXBW0U3cXIe6UjsUHD0pg3gpiiaYcODet1NS2ZRUxGsNdt89Lt2nbgzXF9Kj9E9KnL4Pr1C7cKNbUTU1pIfi4EMRwPUHKqnfJgPfH3zzo5pwxKjYF-uEf6P-n40eUiSGlCO2DGILVIXT3VeoQOrWErpS9ezzIQ9F9ythffwHcLA</recordid><startdate>20140408</startdate><enddate>20140408</enddate><creator>Keefe, Candace C</creator><creator>Goldman, Mildred M</creator><creator>Zhang, Ke</creator><creator>Clarke, Nigel</creator><creator>Reitz, Richard E</creator><creator>Welt, Corrine K</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20140408</creationdate><title>Simultaneous measurement of thirteen steroid hormones in women with polycystic ovary syndrome and control women using liquid chromatography-tandem mass spectrometry</title><author>Keefe, Candace C ; Goldman, Mildred M ; Zhang, Ke ; Clarke, Nigel ; Reitz, Richard E ; Welt, Corrine K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-e676ec2ac1ce36ac988c0ad2418073da9664de48fa8dbdc3734d313a2792469b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>17-alpha-Hydroxyprogesterone - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keefe, Candace C</au><au>Goldman, Mildred M</au><au>Zhang, Ke</au><au>Clarke, Nigel</au><au>Reitz, Richard E</au><au>Welt, Corrine K</au><au>Wilson, Elizabeth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous measurement of thirteen steroid hormones in women with polycystic ovary syndrome and control women using liquid chromatography-tandem mass spectrometry</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-04-08</date><risdate>2014</risdate><volume>9</volume><issue>4</issue><spage>e93805</spage><epage>e93805</epage><pages>e93805-e93805</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The measurement of adrenal and ovarian androgens in women with PCOS has been difficult based on poor specificity and sensitivity of assays in the female range.
Women with PCOS (NIH criteria; n = 52) and control subjects with 25-35 day menstrual cycles, no evidence of hyperandrogenism and matched for BMI (n = 42) underwent morning blood sampling. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to simultaneously measure 13 steroids from a single blood sample to measure adrenal and ovarian steroids. Androgen and progesterone results were compared in the same samples using RIA.
Testosterone, androstenedione, progesterone and 17OH progesterone levels were higher when measured using RIA compared to LC-MS/MS, although the testosterone RIA demonstrated the best agreement with the LC-MS/MS using a Bland-Altman analysis. Results using LC-MS/MS demonstrated that the concentration of androgens and their precursors were higher in women with PCOS than controls [median (2.5, 97.5th %ile); 1607 (638, 3085) vs. 1143 (511, 4784) ng/dL; p = 0.03]. Women with PCOS had higher testosterone [49 (16, 125) vs. 24 (10, 59) ng/dL], androstenedione [203 (98, 476) vs. 106 (69, 223) ng/dL] and 17OH progesterone levels [80 (17, 176) vs. 44 (17, 142) ng/dL] compared to controls (all P<0.02), but no differences in serum concentrations of the adrenal steroids DHEAS, cortisol, corticosterone and their 11 deoxy precursors. Women with PCOS also had an increase in the product:precursor ratio for 3β-hydroxysteroid dehydrogenase [22% (6, 92) vs. 20% (4, 43); p = 0.009].
LC-MS/MS was superior to RIA in measuring androstenedione, progesterone and 17OH progesterone levels, while testosterone measurements were better matched in the two assays. Androgen levels were higher in women with PCOS in the absence of a difference in adrenal-predominant steroids. These data support previous findings that the ovary is an important source for the androgen excess in women with PCOS.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24713888</pmid><doi>10.1371/journal.pone.0093805</doi><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Free; MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | 17-alpha-Hydroxyprogesterone - blood Accuracy Acne Adolescent Adult Androgens Androstenedione Androstenedione - blood Biology and Life Sciences Blood Body mass Body mass index Chromatography Chromatography, Liquid Corticosterone Cortisol Female Hormones Hormones - blood Humans Hydrocortisone Hyperandrogenism - blood Immunoassay Intubation Liquid chromatography Mass spectrometry Mass spectroscopy Medicine and Health Sciences Menstruation Polycystic ovary syndrome Polycystic Ovary Syndrome - blood Progesterone Research and Analysis Methods Scientific imaging Spectroscopy Steroid hormones Steroids Steroids - blood Tandem Mass Spectrometry Testosterone Testosterone - blood Young Adult |
| title | Simultaneous measurement of thirteen steroid hormones in women with polycystic ovary syndrome and control women using liquid chromatography-tandem mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T07%3A48%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20measurement%20of%20thirteen%20steroid%20hormones%20in%20women%20with%20polycystic%20ovary%20syndrome%20and%20control%20women%20using%20liquid%20chromatography-tandem%20mass%20spectrometry&rft.jtitle=PloS%20one&rft.au=Keefe,%20Candace%20C&rft.date=2014-04-08&rft.volume=9&rft.issue=4&rft.spage=e93805&rft.epage=e93805&rft.pages=e93805-e93805&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0093805&rft_dat=%3Cproquest_plos_%3E1514433265%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1513824187&rft_id=info:pmid/24713888&rft_doaj_id=oai_doaj_org_article_604f61d1a24e44cf8a2df2df180a0e9b&rfr_iscdi=true |