Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye
Mini chromosome maintenance 10 (Mcm10) is an essential protein, which is conserved from S. cerevisiae to Drosophila and human, and is required for the initiation of DNA replication. Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (roug...
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| Veröffentlicht in: | PloS one 2014-03, Vol.9 (3), p.e93450-e93450 |
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| creator | Vo, Nicole Taga, Ayano Inaba, Yasuhiro Yoshida, Hideki Cotterill, Sue Yamaguchi, Masamitsu |
| description | Mini chromosome maintenance 10 (Mcm10) is an essential protein, which is conserved from S. cerevisiae to Drosophila and human, and is required for the initiation of DNA replication. Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of dMcm10 knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by dMcm10 knockdown in eye imaginal discs. Surprisingly, when we used deadpan-lacZ and klingon-lacZ enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of dMcm10 by the GMR-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by dMcm10 knockdown. Together, these results suggest that dMcm10 knockdown has an effect on DNA replication and R7 cell differentiation. |
| doi_str_mv | 10.1371/journal.pone.0093450 |
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Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of dMcm10 knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by dMcm10 knockdown in eye imaginal discs. Surprisingly, when we used deadpan-lacZ and klingon-lacZ enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of dMcm10 by the GMR-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by dMcm10 knockdown. Together, these results suggest that dMcm10 knockdown has an effect on DNA replication and R7 cell differentiation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0093450</identifier><identifier>PMID: 24686397</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Animals, Genetically Modified - genetics ; Animals, Genetically Modified - metabolism ; Apoptosis ; Apoptosis - genetics ; Biology ; Biology and life sciences ; Biomedical research ; Cell cycle ; Cell differentiation ; Cell Differentiation - genetics ; Cell division ; Cell Division - genetics ; Chromosomes ; Compound eye ; Deoxyribonucleic acid ; Differentiation (biology) ; DNA ; DNA biosynthesis ; DNA replication ; DNA Replication - genetics ; Drosophila ; Drosophila - genetics ; Drosophila melanogaster ; Drosophila Proteins - genetics ; Eye ; Eye - metabolism ; Genomes ; Imaginal discs ; Imaginal Discs - metabolism ; Insects ; Minichromosome Maintenance Proteins - genetics ; Neurogenesis - genetics ; Ommatidia ; Phenotype ; Photoreceptor Cells, Invertebrate - metabolism ; Proteins ; Replication ; Replication initiation ; Research and Analysis Methods ; Ribonucleic acid ; RNA ; RNA-mediated interference ; S phase ; S Phase - genetics ; Saccharomyces cerevisiae ; Studies ; Yeast</subject><ispartof>PloS one, 2014-03, Vol.9 (3), p.e93450-e93450</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Vo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of dMcm10 knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by dMcm10 knockdown in eye imaginal discs. Surprisingly, when we used deadpan-lacZ and klingon-lacZ enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of dMcm10 by the GMR-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by dMcm10 knockdown. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vo, Nicole</au><au>Taga, Ayano</au><au>Inaba, Yasuhiro</au><au>Yoshida, Hideki</au><au>Cotterill, Sue</au><au>Yamaguchi, Masamitsu</au><au>Bielinsky, Anja-Katrin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-03-31</date><risdate>2014</risdate><volume>9</volume><issue>3</issue><spage>e93450</spage><epage>e93450</epage><pages>e93450-e93450</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Mini chromosome maintenance 10 (Mcm10) is an essential protein, which is conserved from S. cerevisiae to Drosophila and human, and is required for the initiation of DNA replication. Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of dMcm10 knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by dMcm10 knockdown in eye imaginal discs. Surprisingly, when we used deadpan-lacZ and klingon-lacZ enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of dMcm10 by the GMR-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by dMcm10 knockdown. Together, these results suggest that dMcm10 knockdown has an effect on DNA replication and R7 cell differentiation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24686397</pmid><doi>10.1371/journal.pone.0093450</doi><tpages>e93450</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Genetically Modified - genetics Animals, Genetically Modified - metabolism Apoptosis Apoptosis - genetics Biology Biology and life sciences Biomedical research Cell cycle Cell differentiation Cell Differentiation - genetics Cell division Cell Division - genetics Chromosomes Compound eye Deoxyribonucleic acid Differentiation (biology) DNA DNA biosynthesis DNA replication DNA Replication - genetics Drosophila Drosophila - genetics Drosophila melanogaster Drosophila Proteins - genetics Eye Eye - metabolism Genomes Imaginal discs Imaginal Discs - metabolism Insects Minichromosome Maintenance Proteins - genetics Neurogenesis - genetics Ommatidia Phenotype Photoreceptor Cells, Invertebrate - metabolism Proteins Replication Replication initiation Research and Analysis Methods Ribonucleic acid RNA RNA-mediated interference S phase S Phase - genetics Saccharomyces cerevisiae Studies Yeast |
| title | Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye |
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