Percutaneous bone marrow transplantation using fractional ablative Erbium:YAG laser
Topical application of therapeutic agents has been a mainstay in Dermatology for the treatment of skin disorders but is not commonly used for systemic delivery. For a topically applied agent to reach distant body sites it must first overcome the barrier function of the skin and then penetrate into d...
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| Veröffentlicht in: | PloS one 2014-03, Vol.9 (3), p.e93004-e93004 |
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| creator | Rodriguez-Menocal, Luis Salgado, Marcela Davis, Stephen Waibel, Jill Shabbir, Arsalan Cox, Audrey Badiavas, Evangelos V |
| description | Topical application of therapeutic agents has been a mainstay in Dermatology for the treatment of skin disorders but is not commonly used for systemic delivery. For a topically applied agent to reach distant body sites it must first overcome the barrier function of the skin and then penetrate into deeper structures before reaching the systemic circulation. This has limited the use of topically applied agents to those having specific charge, solubility and size restrictions. Pretreatment of the skin with ablative fractional laser appears to enhance the uptake of some topically applied drugs but the ability to effectively deliver agents to distant sites is largely unproven. In this report we used a fractional ablative Erb:YAG (Erbium/Yttrium Aluminum Garnet) laser to facilitate the transfer of bone marrow stem cells through the skin in a murine bone marrow transplant model. Chimerism could be detected in the peripheral blood of recipient C57BL/6 mice that were pretreated with ablative fractional laser and had topically applied enhanced green fluorescent protein (GFP) labeled bone marrow cells from syngeneic donor transgenic mice. This study indicates that fractional laser can be used to deliver stem cells through the skin and remain functionally intact. |
| doi_str_mv | 10.1371/journal.pone.0093004 |
| format | Article |
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For a topically applied agent to reach distant body sites it must first overcome the barrier function of the skin and then penetrate into deeper structures before reaching the systemic circulation. This has limited the use of topically applied agents to those having specific charge, solubility and size restrictions. Pretreatment of the skin with ablative fractional laser appears to enhance the uptake of some topically applied drugs but the ability to effectively deliver agents to distant sites is largely unproven. In this report we used a fractional ablative Erb:YAG (Erbium/Yttrium Aluminum Garnet) laser to facilitate the transfer of bone marrow stem cells through the skin in a murine bone marrow transplant model. Chimerism could be detected in the peripheral blood of recipient C57BL/6 mice that were pretreated with ablative fractional laser and had topically applied enhanced green fluorescent protein (GFP) labeled bone marrow cells from syngeneic donor transgenic mice. This study indicates that fractional laser can be used to deliver stem cells through the skin and remain functionally intact.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0093004</identifier><identifier>PMID: 24667438</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Ablation ; Ablation Techniques - methods ; Aluminum ; Animals ; Biology and Life Sciences ; Bone marrow ; Bone marrow transplantation ; Bone Marrow Transplantation - methods ; Cell cycle ; Chemical compounds ; Chimerism ; Dermatologic agents ; Dermatology ; Drug delivery systems ; Drug dosages ; Drugs ; Erbium ; Female ; Fluorescence ; Gamma rays ; Genetic engineering ; Green fluorescent protein ; Green Fluorescent Proteins - genetics ; Health aspects ; House mouse ; Immunosuppressive agents ; Lasers ; Lasers, Solid-State ; Male ; Medical lasers ; Medicine ; Medicine and Health Sciences ; Mice ; Mice, Inbred C57BL ; Peripheral blood ; Pharmacology ; Rare earth metal compounds ; Research and Analysis Methods ; Skin ; Skin diseases ; Solubility ; Stem cell transplantation ; Stem cells ; Surgery ; Syngeneic grafts ; Topical application ; Transgenic mice ; Transplantation ; Wound healing ; YAG lasers ; Yttrium</subject><ispartof>PloS one, 2014-03, Vol.9 (3), p.e93004-e93004</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Rodriguez-Menocal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Rodriguez-Menocal et al 2014 Rodriguez-Menocal et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-dedd980f8a8de719d0172847ad6d4396a14aaae4aa66b625c59dc13602dc895e3</citedby><cites>FETCH-LOGICAL-c692t-dedd980f8a8de719d0172847ad6d4396a14aaae4aa66b625c59dc13602dc895e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965514/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965514/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24667438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Almeida-Porada, Graca</contributor><creatorcontrib>Rodriguez-Menocal, Luis</creatorcontrib><creatorcontrib>Salgado, Marcela</creatorcontrib><creatorcontrib>Davis, Stephen</creatorcontrib><creatorcontrib>Waibel, Jill</creatorcontrib><creatorcontrib>Shabbir, Arsalan</creatorcontrib><creatorcontrib>Cox, Audrey</creatorcontrib><creatorcontrib>Badiavas, Evangelos V</creatorcontrib><title>Percutaneous bone marrow transplantation using fractional ablative Erbium:YAG laser</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Topical application of therapeutic agents has been a mainstay in Dermatology for the treatment of skin disorders but is not commonly used for systemic delivery. For a topically applied agent to reach distant body sites it must first overcome the barrier function of the skin and then penetrate into deeper structures before reaching the systemic circulation. This has limited the use of topically applied agents to those having specific charge, solubility and size restrictions. Pretreatment of the skin with ablative fractional laser appears to enhance the uptake of some topically applied drugs but the ability to effectively deliver agents to distant sites is largely unproven. In this report we used a fractional ablative Erb:YAG (Erbium/Yttrium Aluminum Garnet) laser to facilitate the transfer of bone marrow stem cells through the skin in a murine bone marrow transplant model. Chimerism could be detected in the peripheral blood of recipient C57BL/6 mice that were pretreated with ablative fractional laser and had topically applied enhanced green fluorescent protein (GFP) labeled bone marrow cells from syngeneic donor transgenic mice. This study indicates that fractional laser can be used to deliver stem cells through the skin and remain functionally intact.</description><subject>Ablation</subject><subject>Ablation Techniques - methods</subject><subject>Aluminum</subject><subject>Animals</subject><subject>Biology and Life Sciences</subject><subject>Bone marrow</subject><subject>Bone marrow transplantation</subject><subject>Bone Marrow Transplantation - methods</subject><subject>Cell cycle</subject><subject>Chemical compounds</subject><subject>Chimerism</subject><subject>Dermatologic agents</subject><subject>Dermatology</subject><subject>Drug delivery systems</subject><subject>Drug dosages</subject><subject>Drugs</subject><subject>Erbium</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Gamma rays</subject><subject>Genetic engineering</subject><subject>Green fluorescent protein</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Health aspects</subject><subject>House 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bone marrow transplantation using fractional ablative Erbium:YAG laser</title><author>Rodriguez-Menocal, Luis ; Salgado, Marcela ; Davis, Stephen ; Waibel, Jill ; Shabbir, Arsalan ; Cox, Audrey ; Badiavas, Evangelos V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-dedd980f8a8de719d0172847ad6d4396a14aaae4aa66b625c59dc13602dc895e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Ablation</topic><topic>Ablation Techniques - methods</topic><topic>Aluminum</topic><topic>Animals</topic><topic>Biology and Life Sciences</topic><topic>Bone marrow</topic><topic>Bone marrow transplantation</topic><topic>Bone Marrow Transplantation - methods</topic><topic>Cell cycle</topic><topic>Chemical compounds</topic><topic>Chimerism</topic><topic>Dermatologic agents</topic><topic>Dermatology</topic><topic>Drug delivery systems</topic><topic>Drug 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For a topically applied agent to reach distant body sites it must first overcome the barrier function of the skin and then penetrate into deeper structures before reaching the systemic circulation. This has limited the use of topically applied agents to those having specific charge, solubility and size restrictions. Pretreatment of the skin with ablative fractional laser appears to enhance the uptake of some topically applied drugs but the ability to effectively deliver agents to distant sites is largely unproven. In this report we used a fractional ablative Erb:YAG (Erbium/Yttrium Aluminum Garnet) laser to facilitate the transfer of bone marrow stem cells through the skin in a murine bone marrow transplant model. Chimerism could be detected in the peripheral blood of recipient C57BL/6 mice that were pretreated with ablative fractional laser and had topically applied enhanced green fluorescent protein (GFP) labeled bone marrow cells from syngeneic donor transgenic mice. This study indicates that fractional laser can be used to deliver stem cells through the skin and remain functionally intact.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24667438</pmid><doi>10.1371/journal.pone.0093004</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| subjects | Ablation Ablation Techniques - methods Aluminum Animals Biology and Life Sciences Bone marrow Bone marrow transplantation Bone Marrow Transplantation - methods Cell cycle Chemical compounds Chimerism Dermatologic agents Dermatology Drug delivery systems Drug dosages Drugs Erbium Female Fluorescence Gamma rays Genetic engineering Green fluorescent protein Green Fluorescent Proteins - genetics Health aspects House mouse Immunosuppressive agents Lasers Lasers, Solid-State Male Medical lasers Medicine Medicine and Health Sciences Mice Mice, Inbred C57BL Peripheral blood Pharmacology Rare earth metal compounds Research and Analysis Methods Skin Skin diseases Solubility Stem cell transplantation Stem cells Surgery Syngeneic grafts Topical application Transgenic mice Transplantation Wound healing YAG lasers Yttrium |
| title | Percutaneous bone marrow transplantation using fractional ablative Erbium:YAG laser |
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