An extended ΔCT-method facilitating normalisation with multiple reference genes suited for quantitative RT-PCR analyses of human hepatocyte-like cells

Reference genes (RG) as sample internal controls for gene transcript level analyses by quantitative RT-PCR (RT-qPCR) must be stably expressed within the experimental range. A variety of in vitro cell culture settings with primary human hepatocytes, and Huh-7 and HepG2 cell lines, were used to determ...

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Veröffentlicht in:	PloS one 2014-03, Vol.9 (3), p.e93031-e93031
Hauptverfasser:	Riedel, Gesa, Rüdrich, Urda, Fekete-Drimusz, Nora, Manns, Michael P, Vondran, Florian W R, Bock, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Algorithms Biology and Life Sciences Cell culture Cell Line Cell lines Endocrinology Gastroenterology Gene Expression Gene Expression Profiling Genes Genomics Hepatocytes Hepatocytes - metabolism Hepatology Humans Kinases Liver Mathematical analysis Medical schools Medicine and Health Sciences Metabolism Methods Physical Sciences Polymerase chain reaction Quenching Ratings & rankings Real-Time Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction - standards Reference Standards Reproducibility Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Stability analysis Studies Transcription
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