Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy
The T-cell immunoglobulin and mucin domain (TIM) family is a relatively newly described group of molecules with a conserved structure and important immunological functions. Identification of Galectin-9 as a ligand for TIM-3 has established the Galectin-9/TIM-3 pathway as an important negative regula...
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| Veröffentlicht in: | PloS one 2014-03, Vol.9 (3), p.e92371-e92371 |
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| creator | Meggyes, Matyas Miko, Eva Polgar, Beata Bogar, Barbara Farkas, Balint Illes, Zsolt Szereday, Laszlo |
| description | The T-cell immunoglobulin and mucin domain (TIM) family is a relatively newly described group of molecules with a conserved structure and important immunological functions. Identification of Galectin-9 as a ligand for TIM-3 has established the Galectin-9/TIM-3 pathway as an important negative regulator of Th1 immunity and tolerance induction. Data about the TIM-3/Gal-9 pathway in the pathogenesis of human diseases is emerging, but their possible role during human pregnancy is not precisely known. The aim of our study was to investigate the number, phenotype and functional activity of TIM-3+ peripheral blood mononuclear cells during healthy human pregnancy.
57 healthy pregnant women [first trimester (n = 16); second trimester (n = 19); third trimester (n = 22)] and 30 non-pregnant controls were enrolled in the study. We measured the surface expression of TIM-3 by cytotoxic T cells, NK cells and NK cell subsets as well as Galectin-9 expression by regulatory T cells by flow cytometry. We analyzed the cytokine production and cytotoxicity of TIM3+ and TIM3- CD8 T and NK cells obtained from non-pregnant and healthy pregnant women at different stages of pregnancy by flow cytometry. Serum Galectin-9 levels were measured by ELISA.
Our results show that the numbers of peripheral NK and cytotoxic T cells and their TIM-3 expression do not change between the first, second and third trimesters of pregnancy. Compared to non-pregnant individuals, regulatory T cells show higher level of Galectin-9 expression as pregnancy proceeds, which is in line with the level of Galectin-9 in the patients sera. Cytotoxic T cells, NK cells and NK cell subsets expressing TIM-3 molecule show altered cytokine production and cytotoxicity during pregnancy compared to non-pregnant individuals.
Our results indicate that Galectin-9 expressing regulatory T cells, TIM-3+ cytotoxic T cells and NK cells could play an important role in the maintenance of healthy pregnancy. |
| doi_str_mv | 10.1371/journal.pone.0092371 |
| format | Article |
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57 healthy pregnant women [first trimester (n = 16); second trimester (n = 19); third trimester (n = 22)] and 30 non-pregnant controls were enrolled in the study. We measured the surface expression of TIM-3 by cytotoxic T cells, NK cells and NK cell subsets as well as Galectin-9 expression by regulatory T cells by flow cytometry. We analyzed the cytokine production and cytotoxicity of TIM3+ and TIM3- CD8 T and NK cells obtained from non-pregnant and healthy pregnant women at different stages of pregnancy by flow cytometry. Serum Galectin-9 levels were measured by ELISA.
Our results show that the numbers of peripheral NK and cytotoxic T cells and their TIM-3 expression do not change between the first, second and third trimesters of pregnancy. Compared to non-pregnant individuals, regulatory T cells show higher level of Galectin-9 expression as pregnancy proceeds, which is in line with the level of Galectin-9 in the patients sera. Cytotoxic T cells, NK cells and NK cell subsets expressing TIM-3 molecule show altered cytokine production and cytotoxicity during pregnancy compared to non-pregnant individuals.
Our results indicate that Galectin-9 expressing regulatory T cells, TIM-3+ cytotoxic T cells and NK cells could play an important role in the maintenance of healthy pregnancy.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0092371</identifier><identifier>PMID: 24651720</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Analysis ; Biology and life sciences ; Blood ; CD56 Antigen - metabolism ; CD8 antigen ; CD8-Positive T-Lymphocytes - metabolism ; Cytokines ; Cytokines - biosynthesis ; Cytometry ; Cytotoxicity ; Cytotoxicity, Immunologic - immunology ; Down-Regulation ; Endocrinology ; Enzyme-linked immunosorbent assay ; Female ; Fetuses ; Flow cytometry ; Galectin-9 ; Galectins - blood ; Galectins - metabolism ; Hepatitis A Virus Cellular Receptor 2 ; Humans ; Hypotheses ; Immune system ; Immunity ; Immunoglobulins ; Immunological tolerance ; Immunology ; Immunoregulation ; Inflammation Mediators - metabolism ; Killer cells ; Killer Cells, Natural - metabolism ; Leukocytes (mononuclear) ; Ligands ; Lymphocytes ; Lymphocytes T ; Lysosomal-Associated Membrane Protein 1 - metabolism ; Medicine and Health Sciences ; Membrane Proteins - blood ; Metabolism ; Middle Aged ; Molecular structure ; Mucin ; Natural killer cells ; Pathogenesis ; Peripheral blood mononuclear cells ; Phenotype ; Pregnancy ; Pregnant women ; Protein Binding ; Research and Analysis Methods ; T cell receptors ; T cells ; Toxicity ; Women's health ; Womens health</subject><ispartof>PloS one, 2014-03, Vol.9 (3), p.e92371-e92371</ispartof><rights>COPYRIGHT 2014 Public Library of Science</rights><rights>2014 Meggyes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2014 Meggyes et al 2014 Meggyes et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-759f31a63c4a303d7e2fd99ca5393062b0cc3135c2bc05b654017258edd778f13</citedby><cites>FETCH-LOGICAL-c692t-759f31a63c4a303d7e2fd99ca5393062b0cc3135c2bc05b654017258edd778f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961322/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961322/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24651720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Bobé, Pierre</contributor><creatorcontrib>Meggyes, Matyas</creatorcontrib><creatorcontrib>Miko, Eva</creatorcontrib><creatorcontrib>Polgar, Beata</creatorcontrib><creatorcontrib>Bogar, Barbara</creatorcontrib><creatorcontrib>Farkas, Balint</creatorcontrib><creatorcontrib>Illes, Zsolt</creatorcontrib><creatorcontrib>Szereday, Laszlo</creatorcontrib><title>Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The T-cell immunoglobulin and mucin domain (TIM) family is a relatively newly described group of molecules with a conserved structure and important immunological functions. Identification of Galectin-9 as a ligand for TIM-3 has established the Galectin-9/TIM-3 pathway as an important negative regulator of Th1 immunity and tolerance induction. Data about the TIM-3/Gal-9 pathway in the pathogenesis of human diseases is emerging, but their possible role during human pregnancy is not precisely known. The aim of our study was to investigate the number, phenotype and functional activity of TIM-3+ peripheral blood mononuclear cells during healthy human pregnancy.
57 healthy pregnant women [first trimester (n = 16); second trimester (n = 19); third trimester (n = 22)] and 30 non-pregnant controls were enrolled in the study. We measured the surface expression of TIM-3 by cytotoxic T cells, NK cells and NK cell subsets as well as Galectin-9 expression by regulatory T cells by flow cytometry. We analyzed the cytokine production and cytotoxicity of TIM3+ and TIM3- CD8 T and NK cells obtained from non-pregnant and healthy pregnant women at different stages of pregnancy by flow cytometry. Serum Galectin-9 levels were measured by ELISA.
Our results show that the numbers of peripheral NK and cytotoxic T cells and their TIM-3 expression do not change between the first, second and third trimesters of pregnancy. Compared to non-pregnant individuals, regulatory T cells show higher level of Galectin-9 expression as pregnancy proceeds, which is in line with the level of Galectin-9 in the patients sera. Cytotoxic T cells, NK cells and NK cell subsets expressing TIM-3 molecule show altered cytokine production and cytotoxicity during pregnancy compared to non-pregnant individuals.
Our results indicate that Galectin-9 expressing regulatory T cells, TIM-3+ cytotoxic T cells and NK cells could play an important role in the maintenance of healthy pregnancy.</description><subject>Adult</subject><subject>Analysis</subject><subject>Biology and life sciences</subject><subject>Blood</subject><subject>CD56 Antigen - metabolism</subject><subject>CD8 antigen</subject><subject>CD8-Positive T-Lymphocytes - metabolism</subject><subject>Cytokines</subject><subject>Cytokines - biosynthesis</subject><subject>Cytometry</subject><subject>Cytotoxicity</subject><subject>Cytotoxicity, Immunologic - immunology</subject><subject>Down-Regulation</subject><subject>Endocrinology</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Fetuses</subject><subject>Flow cytometry</subject><subject>Galectin-9</subject><subject>Galectins - blood</subject><subject>Galectins - metabolism</subject><subject>Hepatitis A Virus Cellular Receptor 2</subject><subject>Humans</subject><subject>Hypotheses</subject><subject>Immune system</subject><subject>Immunity</subject><subject>Immunoglobulins</subject><subject>Immunological tolerance</subject><subject>Immunology</subject><subject>Immunoregulation</subject><subject>Inflammation Mediators - 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metabolism</topic><topic>CD8 antigen</topic><topic>CD8-Positive T-Lymphocytes - metabolism</topic><topic>Cytokines</topic><topic>Cytokines - biosynthesis</topic><topic>Cytometry</topic><topic>Cytotoxicity</topic><topic>Cytotoxicity, Immunologic - immunology</topic><topic>Down-Regulation</topic><topic>Endocrinology</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Fetuses</topic><topic>Flow cytometry</topic><topic>Galectin-9</topic><topic>Galectins - blood</topic><topic>Galectins - metabolism</topic><topic>Hepatitis A Virus Cellular Receptor 2</topic><topic>Humans</topic><topic>Hypotheses</topic><topic>Immune system</topic><topic>Immunity</topic><topic>Immunoglobulins</topic><topic>Immunological tolerance</topic><topic>Immunology</topic><topic>Immunoregulation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Killer cells</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Leukocytes (mononuclear)</topic><topic>Ligands</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Lysosomal-Associated Membrane Protein 1 - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meggyes, Matyas</au><au>Miko, Eva</au><au>Polgar, Beata</au><au>Bogar, Barbara</au><au>Farkas, Balint</au><au>Illes, Zsolt</au><au>Szereday, Laszlo</au><au>Bobé, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2014-03-20</date><risdate>2014</risdate><volume>9</volume><issue>3</issue><spage>e92371</spage><epage>e92371</epage><pages>e92371-e92371</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The T-cell immunoglobulin and mucin domain (TIM) family is a relatively newly described group of molecules with a conserved structure and important immunological functions. Identification of Galectin-9 as a ligand for TIM-3 has established the Galectin-9/TIM-3 pathway as an important negative regulator of Th1 immunity and tolerance induction. Data about the TIM-3/Gal-9 pathway in the pathogenesis of human diseases is emerging, but their possible role during human pregnancy is not precisely known. The aim of our study was to investigate the number, phenotype and functional activity of TIM-3+ peripheral blood mononuclear cells during healthy human pregnancy.
57 healthy pregnant women [first trimester (n = 16); second trimester (n = 19); third trimester (n = 22)] and 30 non-pregnant controls were enrolled in the study. We measured the surface expression of TIM-3 by cytotoxic T cells, NK cells and NK cell subsets as well as Galectin-9 expression by regulatory T cells by flow cytometry. We analyzed the cytokine production and cytotoxicity of TIM3+ and TIM3- CD8 T and NK cells obtained from non-pregnant and healthy pregnant women at different stages of pregnancy by flow cytometry. Serum Galectin-9 levels were measured by ELISA.
Our results show that the numbers of peripheral NK and cytotoxic T cells and their TIM-3 expression do not change between the first, second and third trimesters of pregnancy. Compared to non-pregnant individuals, regulatory T cells show higher level of Galectin-9 expression as pregnancy proceeds, which is in line with the level of Galectin-9 in the patients sera. Cytotoxic T cells, NK cells and NK cell subsets expressing TIM-3 molecule show altered cytokine production and cytotoxicity during pregnancy compared to non-pregnant individuals.
Our results indicate that Galectin-9 expressing regulatory T cells, TIM-3+ cytotoxic T cells and NK cells could play an important role in the maintenance of healthy pregnancy.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>24651720</pmid><doi>10.1371/journal.pone.0092371</doi><tpages>e92371</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2014-03, Vol.9 (3), p.e92371-e92371 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1508983553 |
| source | MEDLINE; Public Library of Science; Full-Text Journals in Chemistry (Open access); PubMed Central; Directory of Open Access Journals; EZB Electronic Journals Library |
| subjects | Adult Analysis Biology and life sciences Blood CD56 Antigen - metabolism CD8 antigen CD8-Positive T-Lymphocytes - metabolism Cytokines Cytokines - biosynthesis Cytometry Cytotoxicity Cytotoxicity, Immunologic - immunology Down-Regulation Endocrinology Enzyme-linked immunosorbent assay Female Fetuses Flow cytometry Galectin-9 Galectins - blood Galectins - metabolism Hepatitis A Virus Cellular Receptor 2 Humans Hypotheses Immune system Immunity Immunoglobulins Immunological tolerance Immunology Immunoregulation Inflammation Mediators - metabolism Killer cells Killer Cells, Natural - metabolism Leukocytes (mononuclear) Ligands Lymphocytes Lymphocytes T Lysosomal-Associated Membrane Protein 1 - metabolism Medicine and Health Sciences Membrane Proteins - blood Metabolism Middle Aged Molecular structure Mucin Natural killer cells Pathogenesis Peripheral blood mononuclear cells Phenotype Pregnancy Pregnant women Protein Binding Research and Analysis Methods T cell receptors T cells Toxicity Women's health Womens health |
| title | Peripheral blood TIM-3 positive NK and CD8+ T cells throughout pregnancy: TIM-3/galectin-9 interaction and its possible role during pregnancy |
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